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    Goat0 5

    Uploaded by

    Choo Wei sheng
    Goat0.0Goat0.0Goat0.0Goat0.0Goat0.0Goat0.0

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    © All Rights Reserved
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    (19) PEA REM EERAR ALA “fp (21) IFS 201310368498, 2 (22) HBA 2013. 08. 22 19) SALA RAMA PEDERI eo ‘eh 250100 2 40 ARC BE ng CD RBA A TRE 2B (51) Int. C1 0120 1/68(2006. 01) C12N 15/11(2006. 01) (56) FT HIE CN 102758025 A, 2012. 10.31, HAA T3 50-51 B. CN 101875976 A, 2010, 11.03, $CAIBER 1. KR 10-2008-0074298 A, 2008. 08.13, 425 Us 2010/0025244 A1, 2010. 02.04, 43. CN 102605090 A, 2012. 07.25, 43 CN 101712996 A, 2010. 05.26, 43% CN 101962675 A, 2011. 02.02, 43 CN 102337328 A, 2012, 02.01, (12) BAAS A eu (10) HALAS CN 103397101 B (45) AVA A 2016.01.13 ERE. ARAYA 16SrRNA Al Cyth SEPA PUMUET RB. GEEFAED . 2008, 98 24 (GEA), 5 259 TEE I 1. 218 261 TL 3 THSEAE . ARAYA LOSHRNA A Cyth EDS A UT AUR © GREER). 2008, 24 98 (FAI), 8 259 THE 8 1. 2 261 TE 3 HAR FIRM SCART GOETH MIPAaT G4) BABAR PP a a LS I BIE Semi HES AAP SM HT 8) HE Ace ARE T — A ALIN fa LE > AA PSA OL Ph SIRE AEG bas ic AE) SE THES Ae AE AYA TE HAE i OU A a ER BE 16S RNA Al Cyt b ARPS HARE RFA 2 SEI FCA 1 Mot 4 ARNE 16S TRNA AL Cyt b RARE AE PCR ATI. POR SHH MNES 373001 4 EL REDS AP PTAC RA, HEHE LOSrRNA BE PL IB HY BES 2 Ee APEUAT MME. ARORA AS AS T bik 4 HARTA. CN 10339710 soe 2 en a a 1103897101 B RW RK + mes 1. BN ADIDE 8 LF ABE SNE 4A PER SO TIE SS HITT, SORMIECET» VALLE SBE APSR BE LOS rRNA Fil cyt b SEEN BASSE, A — at De TC ALIN S| WAT TIA 4 HNN meDNA 16S rRNA EAT PCR AM, FE AD aH a WAH cyt b RPA Tei Slate AN Bly PCR Sr es ABL 3730x1 AEA) aE DPT RAR 16S rRNA AEDT IR Hy BLAC 18 & AS MSDE AT BR AE HE JEP, APN AAMAS SYN + meb-F 257“ TGAGGACAAATATCATTCTG-3" meb-R 25” IAGTTTGTTAGGGATTGATOG- Fi PCR PARMA RAG ¢ 2. Smmol/L dNTP3WL,5XPCR Buffer 2.5L, 10 nmol/L AVAG RLS L6S rRNA IANS] Py # Leh, 10 umol/L AY cyt BIAS Lv L, Tag MFO. 4 WL, DNA BAER 3 HL FFAS PCR S94 1 BZ WE SPF 294°C Smin, 30 4S 45K (H 94°C 308,55'C 30s,72°C 45s, 72°C 20min. 2. ALP PADI i 9 LL 9 AE SATA 4 ASE aR ORME LET SR a Ld RE ELLE RE AAPA meDNA LOS rRNA HEATH HEY FAM S26 RIL, PCR SIM. SM Cyt b REPAY FAM Seba BS] ANAL Feb NS OI FARA + JP, ANOS SIMHF 25” -FAM-AAGAOGAGAAGACCCTTGGACTTTA-3” , SIMER 25” ~GATTGCGCTGTTATCCCTAGGGTA-3" 5 FAM ASS YN + meb-F 25” -FAM—TACK sACAAATA mcb-R 25” ~CCTCCTAGTTTGTTAGGGATTGATOG-3" « 3. AREAL EER 2 HRA FA ak, JURREZET PCR DSM Sh I2S ABISTIOKI 42 ABE DA SHAT SCSI, RAB LOS rRNA SEDRHY SHH BLK HE AS HEME AT BEE CN 108397101 B i mB 1TH FSS ELLE RAE FAS A EBS ICR AS arene AT Coon] xz YE Be Hoy kia Be ACU A — DAL Ee TL AE TALE PAE erie EAS AT HHL BRR [0002] PULA PEI, PEASE AT Ai» PASE AE a AE RE YA Ta. SAY SEE a EAT ARR eS A VBL HP HELD Ye SARA, AT sO EE PLA aT {BL ELSE ATI 0 HEAP AS HET ET ARSE BBL AS TA. 33 FEA BHAA I 5 SE FTO TR WD BESRE 2o ZE— EAA EE SD FESR AN SN ag A CB SEES PDE 2 ATA “RESIN "ATR AB CSE PAN ARSE AS LARS» AS AE DR ATA FAA, HE AN fa BRE A PRR HEE NG AS BH EPS EA HE Tiih, Wa Pets AREER SE. Coos] PAA i) Sal Ay Ts Re HE Sal) BE OPS) DNA OP = NERA. $8 BE WR a HS A HS Sd FT — ZED LF PE HL THY AN A EER BEA SIN HBL SE LL AR TAL SE YRS EE. I 9 a BES BY Fa TS tN Pgh ak ABLIRE AY SE LA BR AN EE ME 9 Coo] DNA feb A 89 FHF Ae BE (sD, HRI YF A LH A tPA (EL FE 0 DNA SPF bia AL BLA DNA % os Ya A a fe a, 5 te A EA OF OE ho BET DNA ESPEN PCR AEE PS FATS A (AE i A: SF SEB PEARSE T PAV A POG HS 9 NY & AH PCR YA, GOV ELE ARE SE 4 MOI NE. HELA AE SEBRLAA HERE PCR REIMF BLAG iy EAL PCR BAY ALBERS LIK EY Ab SI, MB A PrN DOTY SEF, PEPER A, Sas BR ee WG 2 OR A Sa HE AB PAB DO AE TILA ne AE Be EE 5 [0005] AURA ALLA (mt DNA) ZERL AMAA iC SE TE Eg Sees F(A i Bs a RID, HEALS NAL 12S rRNA ELA, 16S rRNA EEL b SETA] (cytochrome b, cyt bb). CEZR IEA DNA "1, L6SxRNA SPA 2 ORY ASHE PLL OE FER & (SE BA, WBA Jae OO PITS Fi THREAD, BEATS FPL, AT RY BL BALE, AE RY DH a A 51, PE FAY] APOE B34 Tid 8 2 iD is RRR, TA AE Co T° BE HAG & PE ARR KAMER, ELA, 16S rRNA IEEE I RTE AEA TY ORE SS Ha Ra ET PE SPRAY mtDNA cyt b REPEAT APT BT HARE AURA. REGIE 1B RL PRAGA S EA, HM SEA aS ARRAN SPADA A De IL) MEL BAT TD Wi2E ABE FAS PIE PA ST II, AS LBB 16S rRNA All cyt b HEA BASE FA, HEAL Sl Poet 4 Aha Yay meDNAIGS rRNA HEFT POR $48, PCR ASHP LE 3730x1 4 FIBER SHAT ACER, DL cyt b SEPA IY AB, HRYE 16S rRNA SEER SA HT BLE & ASPET FRB Heth T EIR 4 ARIE 3 CN 108397101 B i mB 2TH RAAR Coos) Ax Az BHAA) El AMD Je A CP — Fa Fd a LL FS A SEPA HAL HE TARAS AT HITE. (0007) Ax ALAN BEAVER RL TLL SAN BARE FE AY PAM EGC ALBEE DNA LES. rRNA EPL AZAI NG CHK b SEPA CINTA Ro SE FULLER Lid 4 BP ARERR DNA SEPERATE ATT 16S rRNA SEPA BLP BALE ZEAE St, B/D ALE bp, VRC BLP ALB BBL TL RSM S| PATA cyt b SEBEL AE MAS, MES AT AKA. Me SHES ABIST30x1 BEAT EAM Ik, HUE WEA Ae LaE AT A RS 5 «LAL LA RR HR A, BI, SE TRAST PRT MW UDINE SALAM. (ooos] AACA, ALT SMR BEA 16S rRNA SEPRAVIEFEPERE SS, Bib 1 SR S| yl 4, FA FAM S236 ic, Heh UA AY BOY 232bp SEE HY BB BOY 230bp HY El A HT BL 235bp, PSA BAY BUF LN 244bp AVAL cyt b SEPARA EDITS, S14 PAM BEE, BRT ALTE ARAL CIES aH cE AR, HLL ES It FESRSEIA 1 PASSER IE Sl» Cocos] A Ae HARD NAAT nk «AS BQN Ae El HO ALF De eS A SIL SRE AAU SPV BP ALAS OX 9 DINE AR» SRK SEE Se 5] HL He RP, OM RGIS BE. SONG ITER A RA RA I ARIK SEALE AL 51 Pee 7S Be SER RS 4 AAR HDL PR ES, AH TG Be HK, ACR UIE FAM SIGH IGA 16S rRNA SEERA cyt b AEDS AP MOTTA Ew FUT SPAR ALURTE MYER, BATTER PCR SEH, ee AE. AZ, GSA A A TIE BS SEEN, Ga Bh TA AL SEE SS 2 a SAU AR A SPARTA A, SAMRAT ERR T BIE HE (oo1o] ARH ATT ELLE + fort] 1) HEHE 4 4 be ALAA 16S rRNA FER SAS HEML A. Bit HPAL AALS SEP LE A SAPS DH BRAY HHI 232bp . 230bp .235bp. All 244bp » C0012] 2) HUE cyt b MARS WAS. fois] 3) septa xt S| Pie TE S14 5” SHRI O-FAM See beIA, FYE PCR AT HAR. (oora] 4) ASF IHS ABIS73ONL 42 1 BYE PA SY BTA EAM AE MD, AB eee 09 0 BOK Migs AE. (001s) 5) RATE TAL S| MONE EES RH RS (oo1e] 6) ARIAS REET Real. (oor7] 1. ERAS BAL Coors) ar QE UL FAA Fe AUIS AN FP SR TE AEG ACS Se RE LA RE LS, a 2 2 SE ANSEL DNA SER GAR Qiagen Zr] Ex Taq DNA 38 ABR HPL, ANTP. MgC12, DNA 5¢-F ft Marker DL1000, Hi ik LPR PCR AE A I OE SE AE POLAR (KE) FRA) MLDS ATE AT AE ER A OLS) 1 WA DNA RU WARE AA Be BME so cuoowsro Bet (oors] 2. (ka BLA (0020) 42 A BNSF HT{X 3730x1 FFM ABI 2] ;PCR AX Verite96-well H AX Takara ] APR HAIKAX Power PAC200 3 [H Bio-Rad Av] ELMAR AGE Universal HoodI] [4 Bio-Rad 2m] HY BS OBL 5415R 81H Eppendorf AF). Coozt] 3. Ajit H.Ek DNA $21 (ooz2] (HEFT OMEGA 2573] yy 2 DNA HR ASR SR FP HALA AE OE AY UL a Se ATA FEL DNA. 100mg PE i A. DNA SIVA AE-P 100 u LTE S219, Sse T> 260nm 11 280nm Ahly WB GREAEL HSH DNA HEE ATAU IE (oo2s] 4. S1PrRib ANGIE, [0024] RHE GenBank 41 #22 fA LE (REARS MB5541. 1) 482% (HEARS NC_OOI9AL. 1). PS CERACS X60825. 1) ANH CESK 'S AYO20914. 1) BERS 16s rRNA BEIT, Bit Ri AL 519), CER A ESE Pee LP 1. HE ERY cyt b ADL, Bit ATA A eae ERY 51 ot SL Ynity 5” Ha 6-PAM SOIC, BD 16S rRNA HY W-AAGACGAGAAGACCCTTGGAC TPTA~3", 16S rRNA (51613109 SIM-R + ‘ATCC CTAGGGTA-3" scyt b HE Bf IE Fl 51999 meb-F 5” -FAM-TACCATGAG G3", cyt b (YR51Y meb- TA GTTTGTTA TGATCG-3" (0025) 5, Sebi PCR Sa I A [0026] te 25 wL HY POR SS Arar SH AP HEAT LL SE ALS OAD a, AE RAG IRMA (5X) 2.5 UL, dNTP (2, 5mmol / L)3 41, Tag BE 0. 4 u L, Ze BEE 16S TRNA GEL S1497 10 nmol / L) # Le Lscyt Byings i/)51%9 (10 mol / 1) #1 HL, DNA BE BE 3.H Le POR AR AY 294°C Simin, 30 HAI 94°C 308,55°C 308,72°C 458, 72°C 20mine PCR SS a HF 30x] 4 BNET HTT EAM IK, Collection KEK PEWS ELK (SS. 28 a ALEK GeneMapper’3. 2 SH HT BU Ko C0027] 6. Sybase IOiE (ooze) ZN AS ALA SAA AR, SP SARA) 4 PE BRASS DNA Je SEOUL HI a 51D HOURS EEE AT OES be (ooze) 7. SIM Aa ia [0030] #$ 100mg / uw L fi 4 BE PRIZE DNALO fePBB BE RRS, SPST RE UL BE eA TEA ARG BTC STH RA RABI Coost] 8. fori ae Rae Coosa] FUL SAYA AS 5 HALAS AISA ES FP ELI 126 296 5% » REAR Be A FRAP BLAZE RUE [0033] 9. POR Pa Coosa] {MEL 16S rRNA SEAL 510055 HLA HRN AE APSE 4 FH EL BRI DNA, PCR 7* POM Dk Jes BUN AL, SHR A} FERED SB A MEAT TRAE BE 1 SEB Hi BONS HPC UR AE Bed ee OA. SPA RAG GenBank fy Ue LEAT beat. (003s) ARH ALAR Coase] 1) 1 Ane FETE «AC Ac HY ORAL 1 SAS] Yo PU A 4 PAA, SR Ge, TB 5 CN 108397101 B i mB ATH 3p 3 RL PRT ASHER PUY RE TB EST RAS AR TE EL PTFE oA RATES PUNT HE ATA SEI [oos7] 2) Ei PRPS REM TRAE «ACA YT EL da HD 3 PRET Lpe/ RAL. BH POR Gi 2 CRE Be EAA AT A He PIS BEE PCR ERG 1-2 ML FEB. ATT VE TT AT BWP DNA BURP DT A OL [loose] — 3) BME PSS LT ae AE eg AUP) AS AF] A BABEL BI 1 EY PLS, HO a ABP, SE AGLI ABR EL BA AS A RAD» IT ACB HR IE TB PAE RE ASD. Coos] 4) Sp RRE IR ACARI AEG EX 5} BEM Hr BLU) 2 RSA i AP Coos] 5) Bey AS ABR UI (, ey. 6 AIT RE SEL 4 BRP SRTEL PEN Rea. PRE CBB Coogi] FE 1 VURHAZRER RIE 16S rRNA EAP HSL ei PTA. SIMAP TETAS S14 + SINER SCHL 1). REPRE RNS 9 51 28 A AL. (0042) FA 2 EAMG AA IMA. SY. UF. [0043] 3 SEAR GHC OA IMA. MY. AES [0044] 4 ASOLO AT AR. 2. Hie [004s] 5 PYAAR ATA. Y. M [oogs] FH 6 24 BHARTI AT HEAR. SY. USE MY. BASE 92. HY. [0047] FAT TPE R LEA RAS binic SAP eR. SY. UAE GY. AS. ABTHAK (004s) SULIT ANE ARIS FHRYE GenBank HAR AN UIAE CBR M5541. 1) RAE CEAR'S NC_001941. 1HM236185. 1) 5 (EARS X60825. 1) AU (ERS AVIZONLA. 1) 2E BEAK 16s rRNA EDA AP IAL, Beit AL S14), SLE BOE A aE Ae PHS OO ML 1H AE ERMAN cyt b FFL, Beit RAL SIO SRL ARE A S| yh TE Tad 31 A(t 5” SHARIR O-FAM FGF, 16S rRNA FATE TAL 5/ 497 SIM-P 25” -FAM-AAGACGAGAAGACCCTTGGAC TITAS", 16S rRNA BY 5131 Y5 ST GAT ATCCCTAGGGTA-3" seyt b SEDAN IEF 41 9) meb-P "AM-TACCATGAGGACAAATATCATTC 16-3", cyt b (AIL S14%9 mcb—R + 57 -CCTCCTAGTTIGTTAGGGAT TGATCG-3" . [0049] AULA 16s rRNA Al cyt b SER) PAM 52 bai FS] Bore A Aa SM BA By WR Le (ooso] 2 1. FAM S26 bri AL S| FF AL hh AB CN 103397101 B 5TH sy SWE PraKA RAB (3) (bp) SIM-F_FAM-AAGACGAGAAGACCCTTGGACTITA | 1132: 232bp [0051] 44%: 230bp SIM-R_ GATTGCGCTGTTATCCCTAGGGTA, Sis 235 bp BY: 244bp meb-F_FAM-TACCATGAGGACAAATATCATTCTG — 472bp meb-R__ CCTCCTAGTITGTTAGGGATTGATCG [0052] PCR RAZA AA sPCR RBI Ry 25 uw 1, ROME RLG ¢ [0053] ie ETT BXPCR Butter we [2.501 laNTPiamol 7 1 aaT DNN BE aH TOvwol 7 LI6SeRVAP fiw [0054] TOvnol 7 LISSA Jie TOwwol 7 1 wcb-F Tel 10umol / L meb-R lal Taq AM Cer RNase Free A120 lip vas wi [o0ss] PING IHY [0056] CN 108397101 B i mB 67K 95°C Sabb ery 308 SSC 308 307 RC 45h me 2058 [oos7] 48) WHEE PCR IMT HHL ABISTIOx1 Ae By EE SP TREAT EME HK, FA Col Lect ion BCSRAK (BCH HEIR ASG.. PRT AEA FTAA TE GeneMapper3. 2 Sp Fa HBL WSK HE 4 BRA IP pn oo tee oh ED Coos] 5) 4S FRM RGIE

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