PRE-LAB

QUESTIONS1.Rank the
following solutions from least
polar to most polar.Rank on a
scale of 1-4: 1 being the least
polar and 4 being the most
polar.____1___ 50%
ISOPROPANOL / H2O____2___
25% ISOPROPANOL /
H2O____4__ PURE
WATER____3__ ISOPROPANOL
/ H2O2.Identify the analyte,
eluting solvents (mobile
phases), and stationary phase
used inExperiment
1.Analyte:M and M’sEluting
solvent(mobile
phase):0.5%NaCl, 0.2%NaCl
and isopropyl
alcoholStationary
phase:chromatography
paper3.Why would it be
important to know which
food dyes are in food?There
are people in the population
that have sensitivities to
certain food dyes.Itimportant
to know which dyes are in the
food so that they can avoid
those if they have asensitivity
to it.Separation
by
Chromatography
EXPERIMENT 1:
PAPER
CHROMATOGRAPH
YData SheetTable 2: Paper
Chromatography Data and
ObservationsSolventDistance
Traveled byMobilePhase
(mm)Distance Traveledby
Each Analyte(mm)Rf
ValueAdditionalObservations
DistilledWater60mmGreen:1
5mmBlue:12mmRed:12mmG
reen: .25Blue:.20Red:.200.5%
NaClSolution61mmGreen:22
mmBlue:15mmRed:13mmGr
een:.36Blue:.25Red:.210.2%
NaClSolution71mmGreen:14
mmBlue:11mmRed:10mmGr
een:.20Blue:.15Red:.1470%Is
opropylAlcohol25mmGreen:1
5mmBlue:13mmRed:15mmG
reen:.6Blue:.52Red:.6Post-
Lab Questions1.Which
solvent provided the best
separation?The 0.5% NaCl
solution4.Explain which
characteristics of the solvent
were used to effectively
separate theanalytes.The
solvent shouldn’t be too polar
and should not have a high
solubility for the
analyte5.Some children have
reactions to Yellow 5 or
Yellow 6 dye. Yellow 5 is a
pale-yellow colorand Yellow 6
is more orange.Use the colors
seen on the chromatograms
to determinewhich M&Ms®
candies you tested contain
Yellow 5.The green M and M’s
contain Yellow
5.6.Chromatography has
many applications. Research
one application of
chromatographyand explain
how it is used and what
characteristic is utilized for
the separation of
theanalyte(s).Separation
by Chromatography
I am looking at a career in
nutrition.I looked up specific
uses for chromatography in
thenutrition field.Many
companies will use liquid
chromatography to separate
mixtures totest for
contaminants that would lead
to bacterial spoilage. Paper
chromatography hasbeen
used for analyzing food colors
in various drinks, snacks,
jams, and ice creams.Itis used
to make sure that only
permitted food colors are
added to those
foods.Separation
by
Chromatography