Downloaded by: Manager_001 Downloaded on: Apr 14 2023 13:40:10

Trial Trial-Apr142023-1338 Recipe Typ aut Device 251678748292415296114125114204110425

ID 59 name e o ID 216
D

Recipe configuration

Trial start time Trial end time Total Trial Duration Feed to Run as Endpoint
empty safe calculation

Units/ -- -- -- -- -- --
descripti
on

Apr 14, 2023, Apr 14, 2023, 0 days: 00 hour: 00 No no Permeate scale
13:39:12 13:39:55 min:42 sec

Trial configuration

Feed hold up Feed hold up weight Feed start weight Q permeate frequency

Units/ -- g g sec
description

Empty 0 10

-- = Units are not applicable. NA = Feed start weight not entered by user

Endpoint(s), target(s)

Mode Endpoint 1 Endpoint 2 Endpoint 3 Endpoint 4

Endpoint type Diafiltration

Volume

Units / X
description

-- = Units are not applicable.

Main pump

Trial ID:Trial-Apr142023-133859, Page 1 of 10

Main pump

Main pump No of Direction Flow Tubing Delta P Pump Flow Pump Ramp
model Head(s) Control rate ch Rate speed up
ange time,
(%) (1-15
s)

Units/ -- -- -- -- -- mL/mi RPM sec

descripti n
on

KR2i 1 clockwise feed #16 No 200 3

-- = Units are not

applicable.

Trial ID:Trial-Apr142023-133859, Page 2 of 10

Hollow fiber filter and permeate tubing

Hollow Non-Repli Part # Fibercount Fiber ID Surface Extra Model Manufacturer

fiber filter gen Filter area Capillary number name
(Yes/ No) Space (Non-Rep (Non-Replige
(ECS) ligen n Filter)
Filter)

Units/ -- -- -- mm sq cm ml -- --
descriptio
n No C02-E001-0 6 0.5 20.0 0.67
5-N

-- = Units are not NA - User selected

applicable. non-repligen filter

Permea Permeate Holdup Permeate Auto calculate Permeate Permeate Total permeate hold
te Volume used? tube part permeate tubing tube hold up up (ECS + Perm
tubing (Y/N) # holdup? (Y/N) length (tubing) holdup)

Units/ -- -- -- cm ml ml
descrip
tion

no #16 Yes 0

NA = User selected to NA = User selected 'No permeate * If value is zero, this means user
manually enter permeate tubing holdup in system' has selected not to use permeate
tubing holdup holdup in calculations

-- = Units are not

applicable.

Cassette filter and permeate tubing

Cassett Non-Repligen Par Wi Hei Number of Number of Model number Manufacturer

e Filter(Yes/No) t# dth ght cassettes channels (Non-Repligen name
Filter)
(Non-Repligen
Filter)

Units/ -- -- cm cm -- -- -- --
descrip
tion

NA - User
selected
non-repli
gen
cassette

Surface area Total surface area Extra Capillary Space Total ECS (ECS * no of
(ECS, per cassette) cassettes)

Units/ sq m sq m ml ml
description

Trial ID:Trial-Apr142023-133859, Page 3 of 10

 Surface area Total surface area Extra Capillary Space Total ECS (ECS * no of
(ECS, per cassette) cassettes)

-- = Units are not applicable.

Filter plate insert Filter plate insert model number Filter plate insert hold up

Units/ -- ml
description

-- = Units are not applicable. NA - User has not selected filter plate insert model number

Permeate Permeate Permeate tube Auto calculate Permeate tube Permeate hold Total permeate
tubing Holdup part # permeate length up (tubing) hold up (Total
Volume used? tubing ECS + Filter
(Y/N) holdup? (Y/N) plate insert
hold up +
Perm hold up)

-- -- -- cm ml ml

NA = User selected to manually NA = User selected 'No * If value is zero, this means
enter permeate tubing holdup permeate tubing holdup in user has selected not to use
system' permeate holdup in
calculations

-- = Units are not applicable.

Trial ID:Trial-Apr142023-133859, Page 4 of 10

Aux pump 1

Aux pump 1 model Tubing Pump function Flow rate Permeate stop first

Units/ -- -- mL/min --
description

KR1 #16 Diafiltration 1 No

-- = Units are not applicable. Info : Permeate stop first is only for
permeate pump

Aux pump 2

Aux pump 2 model Tubing Pump function Flow rate Permeate stop first

Units/ -- -- --
description

-- = Units are not applicable. NA - Flow rate value is calculated in

real time for this function

ABV 1

Operating pressure control ABV Mod % closed(at Operating ABV

mode Type e start) pressure response

Units/ -- -- -- psi --
descriptio
n

Retentate KR2i auto 50 4 Low

-- = Units are not applicable. NA - No operating pressure

required for manual ABV control

ABV 2

Operating pressure control ABV Mod % closed(at Operating ABV

mode Type e start) pressure response

Units/ -- -- -- --
descriptio
n

-- = Units are not applicable. NA - No operating pressure

required for manual ABV control

Trial ID:Trial-Apr142023-133859, Page 5 of 10

 Retentate tubing Retentate tubing #

Units/ --
description

#16

-- = Units are not applicable.

Recirculation pressure control Recirculation pressure % value

control (Yes/ No)

Units/ -- per minute

description

Yes 25

-- = Units are not applicable. NA - If recirc control is selected as no, % value will be NA

KF KONDUiT

Channel Details value

UV/ AUX Ch 1 no

UV/ AUX Ch 2 no

K Factor Channel 1 1

K Factor Channel 2 1

NA - Set value not Aux = pH/ Turbidity/ Flow rate

defined

-- = Units are not

applicable.

Modified trial -- = Units are not

endpoint(s)/setpoint(s) applicable.

Mode Endpoint 1 Endpoint 2 Endpoint 3 Endpoint 4 Author Timestamp

Endpoint -- --
type

Units/ -- --
description

Trial ID:Trial-Apr142023-133859, Page 6 of 10

Notes
User Name Role Note Text Post By Timestamp

N/A N/A Trial stopped. System (Auto log) Apr 14, 2023, 13:39:55

N/A N/A D mode 1 ended, Diafiltration Volume 2.00 X System (Auto log) Apr 14, 2023, 13:39:55

Manager_001 Manager Trial stop button pressed Sushant jha Apr 14, 2023, 13:39:55

N/A N/A Ramp up done. System (Auto log) Apr 14, 2023, 13:39:40

N/A N/A Trial resumed. System (Auto log) Apr 14, 2023, 13:39:37

Manager_001 Manager Trial resume button pressed Sushant jha Apr 14, 2023, 13:39:37

N/A N/A Trial paused (main pump in recirculation) System (Auto log) Apr 14, 2023, 13:39:31

Manager_001 Manager Trial pause button pressed Sushant jha Apr 14, 2023, 13:39:31

N/A N/A Ramp up done. System (Auto log) Apr 14, 2023, 13:39:14

Manager_001 Manager Trial Start Sushant jha Apr 14, 2023, 13:39:12

N/A N/A D mode 1 started, Diafiltration Volume 0.00 X System (Auto log) Apr 14, 2023, 13:39:11

N/A N/A Permeate scale taring complete. System (Auto log) Apr 14, 2023, 13:39:11

N/A N/A Permeate scale taring started. System (Auto log) Apr 14, 2023, 13:39:10

Manager_001 Manager Sets Q permeate frequency 10 second Sushant jha Apr 14, 2023, 13:39:10

Manager_001 Manager Save Trial Sushant jha Apr 14, 2023, 13:39:10

Trial ID:Trial-Apr142023-133859, Page 7 of 10

Alarms
Alarm description Alarm value Nature Timestamp

Trial ID:Trial-Apr142023-133859, Page 8 of 10

Trial tables
TPW = Total permeate weight
K Ch 1 = Conductivity Ch 1
PT (holdup) = Permeate total with holdup

Timestamp Duratio P feed P retentate P TMP Q feed Q M feed TPW

n permeate permeate

-- hh:mm: psi psi psi psi mL/min mL/min g g

ss

Apr 14, 00:00:0 -12.510 0.390 0.390 -6.450 66.66 0 2007.1 0.0
2023, 1
13:39:13

Trial ID:Trial-Apr142023-133859, Page 9 of 10

Trial tables
Timesta Duration ABV 1 ABV 2 Aux Aux K Ch 1 K Ch 2 Temp Temp Flow Turbi
mp pump 1 pump 2 Ch 1 Ch 2 mete dity
r Ch Ch 1
1

hh:mm:s % % mL/min -- mS/cm mS/cm C C mL/ NTU

s min

Apr 14, 00:00:01 0.00 N/A 0 0 N/A N/A N/A N/A N/A N/A
2023,
13:39:13

Software Version: KF Comm v3.2.4

Trial ID:Trial-Apr142023-133859, Page 10 of 10