1: Lipopolysaccharide Inhibts or Accelerates Biomedical Titanium Corrosion Depending

on Environmental Acidity
● Biofilm in peri-implant environment: Acute acidification usually pH 4-6
● Surface morphology unchanged by LPS
● Proposed Mechanisms
○ LPS binding to surface blocks ion release to environment (pH<4)
○ Ti complex formed with LPS leads to a thinner passive layer (pH 4-7)
● Clinical Implications
○ Skin and Mucosa penetrating implants have implicit biofilm
○ LPS may increase the passive current density = unwanted corrosion

2: Bioglass/carbonate apatite/collagen composite scaffold dissolution products promote

human osteoblast differentiation
● Hypothesis: bioactive ions released from ossimend bioactive would favor faster primary
osteoblast differentiation and ECM mineralization
● Combination of Si from bioglass and the bioactive ions P and Ca allow for composites to
stimulate osteoblas differentiation and ECM mineralization, leading to faster bone in-
growth
3: Cell migration regulated by RGD nanospacing and enhanced undermoderate cell
adhesion on biomaterials
● 70nm critical adhesion
● Less than 70nm RGD spacing = cant form leading edge
● More than 70nm RGD spacing = can’t form trailing edge
4: Effects of substrate stiffness on the tenoinduction of human mesenchymal stem cells
● 10MPa aligned matrix best for tenoinduction
● Mimicking the directionality of the matrix and the stress on the ECM makes this
differentiate the way we want better
5: Porous PEEK improves bone-implant interface compared to plasma-sprayed titanium
coating on PEEK
● Conventional PEEK implants typically possess a smooth surface due to injection
molding or machining which limits osseointegration compared to roughened and porous
surfaces
● Smooth PEEK < Plasma-sprayed titanium coatings on PEEK < porous PEEK
● Macro-porous features exhibiting improved functional osseointegration
6: Simple Surface Engineering of PDMS with Polydopamine for Stabilized Mesenchymal
Stem Cell Adhesion and Multipotency
● PDMS is easy, transparent, and has precision but is hydrophobic
● Polydopamine coating - decreases in vivo toxicity and reduces surface hydrophobicity
● Increse long term stem cell adhesion and differentiation = better models
● Surface wettability is unlikely to be THE critical factor in BMSC culture proliferation
● PD/collagen coating will enhance stem cell differentiation in microfluidic systems
7: Sequential Application of Topographical Patterns Enhances Derivation of Funtional
Mesencephalic Dopaminergic Neurons from Human Induced Pluripotent Stem Cells
 ● Use sequential topographies to differentiate DA Neurons from iPSCs to make more
efficient models
● Methods
○ PDMS mold silanized and human iPSC
○ Flow cytometry had midbrain DA markers on patterned surfaces and marker
○ TUJ1-positive cells
● Grating sizes
8: Directing cell migration and organization via nanocrater-patterned cell repellent
interfaces
● Multiphoton ablation lithography to create spacing-gradient nanocrater-patterned
surfaces
● Investigation of cell adhesion and migration on quartz surfaces with different spacings &
patterns of nanocraters
○ Changing Nanocrater pitch: multiphoton ablation lithography
○ Striped
○ Gradient pattern
● Patterning dependence on cell migration:
○ Still need myosin and actin to bind
9: Multiparametric atomic force microscopy imaging of single bacteriophages extruding
from living bacteria
● Previous: slow and low resolution; Advance: peakforce tapping by functionalizing tip with
Ni-NTA groups to detect His-tagged particles = location sites of phage assembly and
release.
● Methods
○ Phage +Plasmid = multiple phages from one bacterial cell
○ Fluorescence imaging
○ Cantilever was Cr then coated with Au then bound to Ni2+
● Single Bacteriophage: adhesion events sounds
○ Max adhesion is where the His-Ni-NTA bond ruptures
○ Bacteriophages appear clustered and make soft nanodomains
10: Bone Surface Mimicked PDMS Membrane stimulate osteoblasts and calcification of
bone matrix
● Native-like bone mimicked surface by transfer bone surface topographical properties
onto PDMS by soft lithography more closely than electrospinning or CAD
● mRNA levels had higher OSX oSC, COL1A1, and ALP than unmodified
● Functionalized with fibronectin = decrease hydrophobicity
11: A confined-etching strategy for intrinsic anisotropic surface wetting patterning
● mask-free substrate etching using ink-jet printing and control of confined decomposition
of material surfaces
● Chemical wet etching: direction is controlled by crystallin properties of the substrate
(material removal process)
● Water Contact Angle (WCA) quantifies the surface energy and contact between a liquid-
solid interface (Anisotropic surface wettability = directionally dependent) and can be
manipulated by altering the surface (Coating, Etching, Substrate)
 ● Cellulose triacetate film (HC). etched away in less than 10 minutes after ink layer is
patterned (info storage and encryption)
● Flexible electronics: repeated bending without altering electrical properties
○ NaOH penetration into porous CTA surface accelerated by alcohol prewetting
12: Plasma polymerized nanoparticles effectively deliver dual siRNA and drug therapy in
vivo
● Plasma polymerized nanoparticles to codeliver therapeutic cargo to overcome cell-drug
resistances
● PPNs were just seen as a byproduct of plasma coating but can make sphere-like
nanoclusters of CN:H with a still reactive surface which could be functionalized
● Come from a sterilization source so less processing is needed.
○ Delivered combo siVEGF and Paclitaxel at lesser doses and reduced tumor
growth
13: Bypassing Adverse Injection Reactions to Nanoparticles Through Shape Modification
and Attachment to Erythrocytes
● Compare shapes of nanoparticles or their attachment to erythrocyte surface
● Higher pulmonary macrophages = more sensitive to nanoparticles… maybe be able to
bypass the macrophage interaction = lessen change of Pulmonary Arterial Pressure
which could change the drug
● Hitchhiking: lower amount of PAP activation and unbound particles had increased
thromboxane levels
14: Localized Immune Tolerance from FasL-Functionalized PLG
● Immunomodulation via PLG-biotin microporous scaffolds bound with SA-FasL to protect
allogenic islet transplantation from adverse effects from the immune system without
continued immunosuppression to prevent host rejection
● Rapamycin treament.
● Grafts able to normalize for 200 days after reatment stopped.
● Other molecules on an allogenic bone graft could reduce the potential of T cell activation
15: Dual-color magnetic-quantum dot nanobeads as versatile fluorescent probes in test
strip for simultaneous point-of-care detection of free and complex prostate-specific
antigen
● Fluorescent probes using magnetic-quantum dot nanobeads (MQBs) for detecting
prostate specific antigen
● Method
○ Solvothermal reaction to make MQBs then deposit on ultrathin positively-charged
PEI layer
○ Absorb green and red QDs via carboxyl bonds
● Able to have a low detection
● Variation of a Lateral Flow Immunoassay (LFIA) to detect two different bound antigens
and compare their levels
16: A nanoparticle-based strategy for the imaging of a broad range of tumours by
nonlinear amplification of microenvironment signals
● Molecular imaging with pH sensitive nanoprobes which detect pH and angiogenesis
● Three components:
 ○ Ultra pH sensitive core
○ Series of fluorophores with a large emission spectrum range
○ Targeting unit like RGD
● More particles can be delivered. Can target multiple tumors at once