TITLE: COMPLEXOMETRY TITRATIONS

COURSE: ANALYTICAL AND ENVIRONMENTAL CHEMISTRY

NAME: THANUSSHA KUMARAPPAN
METRIC NO.: S67691
GROUP MEMBERS: LOW LILI
NURUL SYAMIMI BINTI AHMAD SIDI
DATE: 9/1/23
DEMONSTRATOR: DR SITI SOFO BT ISMAIL
 LAB REPORT AEC3012
Title of Experiment: Complexometry Titrations
Date: 9/1/2023 Marks
Name: THANUSSHA A/P KUMARAPPAN Matric No: S67691
Demonstrator DR SITI SOFO BINTI ISMAIL
:
Group 1. LOW LI LI
Members 2. NURUL SYAMIMI BINTI AHMAD SIDI
3.

Introduction
Reaction with respect to the formation of complexes can be used in volumetric
analysis of various types of metal cations. Titration where the main reaction occurs
to form a complex is called a complex titration. An example is the titration of zinc
and EDTA. EDTA is ethylene diamine tetra acetic acid.

This salt is a white solid and when it is dissolved in water, Na+ ions, anions and
water molecules will separate. If the ammonia solution is added, the COOH groups
in the anion will eliminate their H+ ions and ions (EDTA)4- will form complexes, for
example: it will react with zinc ions to form complexes [Zn (EDTA)]2- as below: Zn
2+ + (EDTA)4 - [Zn (EDTA) ]2 - .

Methodology 1. PREPARATION OF EDTA SOLUTION

Weigh accurately
approximately 0.95 g
disodium EDTA dihydrate salt.

Transfer to a 250 ml
volumetric flask.

Dissolve with distilled water

and dilute to graduation level.
Shake until homogeneous.
2. DILUTION AND STANDARDIZATION OF EDTA SOLUTION

Pipette 25 ml solution of calcium

carbonate (0.01M) into a 250 ml
Erlenmeyer flask.

Dilute to approximately 100 ml. Add 2

ml solution of magnesium complex, 5
ml of buffer solution and 1-2 drops of
indicator into this solution.

Titrate with EDTA until the dark red

color turns to blue.

Repeat the titration twice.

Repeat titration without adding 2mL

magnesium complex solution (Record
your observation)

3. DETERMINATION OF THE CONCENTRATION OF ZN+ SOLUTION

 Pipette 25 ml of prepared Zn2+
solution into a 250 ml Erlenmeyer
flask

Dilute to approximately 100 ml.

Add 10 ml buffer solution and 1
drop indicator solution into this
solution.

Titrate with EDTA standard solution

until the color changes from dark
red to blue.

Titrate slowly and carefully when

approaching the end point.

Repeat the titration twice

Data 1. Fill in your data in the given table below:

Tabulation
i. The mass of disodium EDTA dihydrate salt = 0.95g
ii. Dilution and standardization EDTA

With 2 mL Mg complex Without 2 mL Mg complex

Trial 1 Trial 2 Trial 3 Trial 1 Trial 2 Trial 3
Initial 23.4 37.3 0.4 13.6 16.2 16.9
reading
Final reading 37.3 52.0 13.6 14.5 16.9 17.7
EDTA volume 13.9 14.7 13.2 0.9 0.7 0.8
(mL)
Observations Red turns to blue slower Red turns to blue faster

iii. Determination of the concentration of Zn2+ solution.

Trial 1 Trial 2 Trial 3

Initial reading 0.0 18.2 33.0
Final reading 14.0 33.0 47.0
 EDTA volume (mL) 14.0 14.8 14.0

2. Calculate the concentration of prepared EDTA and the concentration of

EDTA standardized with calcium carbonate in molar unit.

0.95
mole =
336.2
= 2.825×10-3 mol

mass
concentration of =
volume
prepared EDTA
2.825× 10
=
0.25
= 0.0113 M

concentration of EDTA standardized

13.9+14.7+13.2
average of volume EDTA with Mg complex =
3
= 13.933 mL

0.9+0.7+ 0.8
average of volume EDTA without Mg complex =
3
= 0.8 mL

volume of EDTA = volume titrated with Mg complex - volume titrated

without Mg complex
=13.933 – 0.8
=13.133 mL

MEDTAVEDTA = MCaCO3VCaCO3
MEDTA(0.01313) = (0.01)(0.025)
MEDTA = 0.019 M

3. Calculate the concentration of zinc solution in the gdm -3 unit.

14.0+14.8+14.0
average of volume EDTA =
3
= 14.267 mL
MZnVZn = MEDTAVEDTA
MZn(0.025) = (0.019)(0.0143)
MEDTA = 0.0109 M

concentration of Zn = 0.0109×65.38
= 0.7126 gdm -3

Data
Interpretation 1. What is the function of the buffer solution for titration with EDTA?
and Brief Resists the change in pH
Discussion
 2. Why the concentration of obtained EDTA should be standardized with a
solution of calcium carbonate?
The CaCO3 solution will help to determine the end point

3. Give three examples of other indicators used in titration with EDTA.

-Eriochrome Black T
-Fast Sulphon Black
-Patton Reeder

4. Why magnesium complex solution should be added into a calcium

solution before titrated with EDTA? Explain your answer.
In order to see the change in colour from pink to blue, when all the
magnesium has disappeared from water. In addition, magnesium complex
is less stable than calcium complex. So all calcium ions are transformed into
stable complex with EDTA, liberating magnesium ion that associate to
indicator to show pink colour.

5. Propose 4 precautions to be taken when doing this experiment.

-Do not touch or taste any unknown substance.
-Use an only a small quantity of chemicals to carry out experiment.
-Do not work alone in the laboratory.
-Do not throw hot concentrated acids into the sink directly.

6. Describe three factors that cause results not meet the practical theory.
- slow rate
-decomposition of reactant
-evaporation

Conclusion

We able to do the dilution and standardization of EDTA solution. The concentration

of EDTA standard solution and the concentration of zinc has been calculated.

References Why is buffer solution used in EDTA titration? (2020, February 3).
ElegantQuestion.com. Retrieved January 10, 2023, from https://elegant-
question.com/why-is-buffer-solution-used-in-edta-titration/

Which indicator is used in EDTA titration? (2019, August 16). Sage-Advices.

Retrieved January 11, 2023, from https://sage-advices.com/which-indicator-is-
used-in-edta-titration/

Why should magnesium complex solution be added into a calcium solution before
titrated with EDTA? (n.d.). Quora. https://www.quora.com/Why-should-
magnesium-complex-solution-be-added-into-a-calcium-solution-before-titrated-
with-EDTA

Charco, A. (2008, September 25). Which factors determine the percentage yield in a
laboratory preparation? – Write Charlie’s iblog.
http://www.ibchem.com/faq/2008/09/25/whic-factors-determine-the-percentage-
yield-in-a-laboratory-preparation/