EXERCISE - I

BIOMOLECULES -2022

A. Single correct question (M.C.Q) (1 to 50)

1.Match column I with Column II given below: (Important one)

Column I Column II

P. Spider Web i)Sericin

Q. Silk Fiber ii)Elastin

R. Cotton iii)Collagen

S. Hair iv)Cellulose

T. Ligament v)Keratin

U. Tendon vi)Fibroin

Choose the correct Combination-

A) P-vi Q-i,vi R-iv S-v T-ii U-iii

B) P-i,vi Q-i R-iv S-v T-ii U-iii

C) P-i Q-vi R-iii S-v T-iv U-ii

D)P-i Q-iv R-iii S-v T-ii U-ii,iii

2. Which one of the following is not present in Colostrum?

A) Protein
B) Antibody
C) Carbohydrate
D) Hemoglobin

3. The number of Correct statements amongst the given statements is :

A) Amino acids are substitute of methane

B) . The R group in serine is hydroxyl-methyl.
C) Arachidonic acid has 20 carbon atoms including the carboxyl carbon.
D) Glycerol is trihydroxypropane.
E) Lecithin is a phospholipid.

Choose the correct option-

1. 2
2. 3
3. 4
4. 5
4.Ethanol is used to treat methanol toxicity because ethanol - (Important one)
1. Is a competitive inhibitor of alcohol dehydrogenase
2. Is a non-competitive inhibitor of alcohol dehydrogenase
3. Activates enzymes involved in methanol metabolism
4. Inhibits methanol uptake by cells

5. Which one of the following amino acids is least likely to be in the core of a protein?

1.Arginine

2.Phenylalanine

3. Isoleucine

4. Valine

6. What kind of secondary metabolites are Morphine and Concanavalin A respectively?

1.Lectins,Alkaloids

2.Tarpenoids, Drug

3.Alkaloids,Lectins

4. Toxin,Alkaloids

7. Which of the following amino acids contains Hydroxyl Group (-OH) ?

1. Cysteine
2. Tryptophan
 3. Serine
4. Glutamate

8.Which ONE of the pairs of amino acids contain two chiral centres?

1. Lysine and Valine

2. Isoleucine and Threonine
3. Valine and Isoleucine
4. Lysine and Threonine

9.Which of the following species of any amino acid will be dominant at pH = 7.0 ?

1.

2.
3.

4. All of the above

10.The following graphs with the solid and dotted lines correspond to the reactions without and
with enzymes, respectively. Which of the following graph(s) correctly represent the concept of
activation energy?
Choose the correct option-

1. ii
2. iii,iv
3. i,ii
4. ii,iii

11. The immediate precursor of thyroxine is which amino acid-

1. Tyrosine
2. Tryptophan
3. Phenylalanine
4. Histidine
12.Choose the CORRECT combination of statements given below related to a cysteine residue in
proteins.

i. Cysteine can be linked to tyrosine by S-O bond.

ii. Cysteine can be linked to another cysteine by S-S bond.

iii. Cysteine can complex with Fe2+

iv. Cysteine can be linked to methionine by S-S bond

1. i and ii
2. ii and iii
3. iii and iv
4. i and iv

13.What is the length of human DNA containing 7.2 X 109 bp? (Where bp=Base Pair)

1. 2.44 m
2. 24 nm
3. 2.44 nm
4. 24 m

14.Which is the cofactor of Carboxypeptidase?

1.Iron

2.Cobalt

3.Zinc

4.Manganese

15.
Match the enzymes in Group I with the reactions in Group II. Select the correct combination.

Group 1 Group 2

P Hydrolase (i) Inter-conversion of optical isomers

Q Lyase (ii) Oxidation and reduction of two substrates

R Isomerase (iii) Joining of two compounds

S Ligase (iv) Removal of a chemical group from a substrate

(v) Transfer of a chemical group from one substrate to another

1. a) P-(iv), Q-(ii), R-(iii), S-(i)

2. b) P-(v), Q-(iv), R-(i), S-(iii)
3. c) P-(iv), Q-(i), R-(iii), S-(v)
4. d) P-(i), Q-(iv), R-(v), S-(ii)

16.Given below are the four schematics that describe the dependence of the rate of an enzymatic
reaction on temperature. Which of the following combinations is true for thermophilic and
psychrophilic organisms?

Choose the correct option-

 1. P,Q
2. R,S
3. R,R
4. S,S

17.Maltose is a polymer of -

1. Glucose + Fructose
2. Glucose + Glucose
3. Glucose +Galactose
4. Glucose + Ribose

18. The sequences of four DNA molecules are given below-

Which one of these DNA molecules will have the highest melting temperature (Tm) ?

I. TATATATATATATATA

ATATATATATATATAT

II. AAAGCGCTTTATGC

TTTCGCGAAATACG
III. TAGCTAGCCGAGC

ATCGATCGGCTCG

IV. GCCGGGGCCCCG

CGGCCCCGGGGC

Choose the correct option-

1. I
2. II
3. III
4. IV

19. Molecular Scissor is the enzyme that cleaves DNA molecules into smaller fragments.Which
type of bond do they act on?

1. Hydrogen Bond
2. Disulphide Bond
3. N-Glycosidic Bond
4. Phosphodiester Bond

20. Glycogen is a branched chain polymer of

α-D glucose units in which chain is formed by C1-C4 glycosidic linkage whereas branching
occurs by the formation of C1-C6 glycosidic linkage. Structure of glycogen is similar to ..............
.

1. amylose
2. amylopectin

3. cellulose

4. glucose

21. A protein called A with isoelectric point 6.9 was isolated.It was prepared in three different
buffers M,N,P with three different pH 3.3,6.9,8.6 respectively.The net charge on Protein A ,the
correct option is-

1. Net charge in both M,N is positive and negative in P

2. Net charge in M is positive and in P is negative while both positive and negative charges
are absent in N
3. Net charge in M is positive and P is negative ,while both positive and negative present on
N.
4. Net charge in M is positive and negative in Both N,P.

22.A researcher determined the initial velocity (Vo) of an enzyme in three different reactions
(corresponding to lines P, Q and R. in the graph) with increasing substrate concentrations ([S]).
The results were analyzed using a reciprocal plot where 1/V0 is plotted against 1/[S](Graph).
Reaction 1 was performed without any inhibitory compound. Reaction 2 was performed with
compound-X that inhibits enzyme activity by binding to the substrate binding site. Reaction 3
was performed with compound-Y that inhibits enzyme activity by binding to a site other than the
substrate binding site. If Km of the enzyme in reaction 1 is 0.5 mM, then the correct statement(s)
from the following options is(are) -
 A. Reaction 1 is P, Reaction 2 is Q and Reaction 3 is R
B. Reaction 1 is R ,Reaction 2 is P and Reaction 3 is Q
C. Reaction 1 is R ,Reaction 2 is Q and Reaction 3 is P
D. Km of Reaction Q is less than Km of reaction R

Choose the correct option-

1. A,B
2. B,D
3. B,C
4. A,D

23. Which type of inhibition is shown the following graph-

 1. Non-competitive Inhibition
2. Uncompetitive Inhibition
3. Competitive Inhibition
4. None of the above

24. Which of the following are cofactors?

1. Co-enzyme
2. Metal Ions
3. Prosthetic Group
4. All of these

25. The organic substance bound to an enzyme and essential for its activity is called :

1. Coenzyme
2. Holoenzyme
3. Apoenzyme
4. Isoenzyme
26. In competitive Inhibition :

1. Inhibitor resembles the substrate in molecular structure

2. Competitive between substrate and inhibitors to occupy active sites
3. Binding the inhibitors to activate sites declines the enzyme action
4. All of these are correct.

27. Given below is the graph showing the effect of substrate concentration on enzyme activity.In
the presence of competitive inhibitor,when the concentration of the substrate is progressively
increased :

1. The Km value is increased but the reaction will not achieve Vmax
2. The Km value is increased but the reaction can ultimately achieve Vmax
3. The Km value is decreased but the reaction will not achieve Vmax
 4. The Km value is decreased but the reaction can ultimately achieve Vmax

28. The graph given below shows the Michaelis-Menten kinetics for two reactions (R1 and R2;
represented as solid and dotted lines, respectively). Enzymes El and E2 have Vmax values,
Vmax 1 and Vmax 2 respectively. The respective KM values for El and E2 are KM1 and Km2.
(KM1 = KM2).

A. E1 ,E2 can never be same enzyme

B. E1, E2 may be the same enzyme ,acting on two different substrates with different
affinities.
C. E1 and E2 may represent two different concentrations of the same enzyme ,acting on the
 same substrate.
D. E1 and E2 may be two different enzymes,with the same affinity for the substrate.

Choose the correct option-

1. A,C
2. B,C
3. C,D
4. B,C

29.Which bond forms between 1 glucose and 1 galactose molecule to form Lactose and between
which carbons?

a. Peptide bond ; C1-C4

b. Phosphodiester bond ; C1-C2
c. Glycosidic bond ; C1-C4
d. Glycosidic ; C1-C2

30. Match The Following :

a) Aquaporin i) Amide
b) Asparagine ii) Polysaccharide
c) Abscisic acid iii) Polypeptide
d) Chitin iv) Carotenoids

Select the correct option :

1) a-iii ,b-i , c-iv ,d-ii

2) a-ii,b-iii,c-iv ,d-i

3. A-ii ,b-i,c-iv,d-iii

4.a-iii,b-i,c-ii,d-iv
31. Match the following :

a) Inhibitor of catalytic activity i) Ricin

b) Possess peptide bonds ii)Malonate
c) Cell wall material of fungi iii)Chitin
d) Secondary Metabolite iv) Collagen

Choose the correct option from the following :

1. a-ii,b-iv,c-iii,d-i
2. a-i,b-iv,c-iii,d-ii
3. a-iv,b-ii,c-iii,d-i
4. A-ii,b-i,c-iii,d-iv

32.Regarding factors affecting enzyme activity :

I. Low temperature preserves the enzyme in a temporarily inactive state whereas high
temperature destroys enzymatic activity.
II. With the increase in substrate concentration,the velocity of the enzymatic reaction rises at
first.

Choose the correct option-

1. Only I is correct
2. Only II is correct
3. Both I and II is correct
4. Both I and II is incorrect

33.Which one of the following structural formulae of two organic compounds are correctly
identified along with its related function?

1. A-Triglyceride-Sources of energy e-major

2. B-Uracil-a component of DNA
3. A-Lecithin-a component of cell membrane
4. B- Adenine-a nucleotide that makes up nucleic acids

34.The given structure represents-

 1. Glycolipid
2. Steroid
3. Phospholipid
4. Lipoprotein

35.Following are the statements with reference to 'lipids'.

(a) Lipids having only single bonds are called unsaturated fatty acids.

(b) Lecithin is a phospholipid.

(c) Trihydroxy propane is glycerol.

(d) Palmitic acid has 20 carbon atoms including carboxyl carbon.

(e) Arachidonic acid has 16 carbon atoms.

Choose the correct answer from the options given below.

1. (b) and (c) only

2. (b) and (e) only

3. (a) and (b) only

4. (c) and (d) only

36.Carbohydrates are commonly found as starch in plant storage organs. Which of the following
five properties of starch(A-E) make it useful as a storage material?

(A) Easily translocated

(B) Chemically non-reactive

(C) Easily digested by animals

(D) Osmotically inactive

(E) Synthesized during photosynthesis

The useful properties are

1. (B) and (C)

2. (B) and (D)

3. (A), (C) and (E)

4. (A) and (E)

37.Inulin is a polymer of _________________ and Chitin is a polymer of

___________________ respectively.
Choose the correct option:

1.Fructose,Glucose

2. Glucose,Glucose

3. Fructose,Glucosamine

4.Glucose,Glucosamine

38. The compound shown below-

1. Is most commonly found in the ori region of DNA

2. Can have enzymatic activity
3. Is a component of ATP molecule
4. May be a component of cell membrane.

39. Which of the following organic molecules is not correctly matched with diagrammatic
representation of its structure?
Choose the correct option-

A. 1
B. 2
C. 3
D. 4

40.Given below is the diagrammatic representation of one of the categories of small molecular
weight organic compounds in the living tissues. Identify the category shown and the one blank
component X in it -
Category Component

1. Cholesterol Guanin

2. Amino acid NH2

3. Nucleotide Adenine

4. Nucleoside Uracil

41. An example of protein with quaternary structure?

1. Myoglobin
2. Hemoglobin
3. Keratin
4. All of these.

42.A typical fat molecule is made up of

1. 1 glycerol and 3 fatty acid molecule

2. 3 glycerol and 1 fatty acid molecule
3. 3 glycerol and 3 fatty acid molecule
4. 1 glycerol and 1 fatty acid molecule
43.

I. Segment representing the energy activation

II. Segment representing the amount of free energy released by the Rection
III. Transition State
IV. Segments would be the same regardless of whether the reactions were non-catalysed or
catalyzed.

Which one is correct?

I II III IV

a 1 3 2 4
 b 1 2 3 2

c 1 3 2 4

d 1 2 4 3

A. a
B. b
C. c
D. d

44. Which of the following is the correct enzyme catalyzed reaction?

1. Substrate is converted into enzyme

2. Substrate is consumed in the reaction completely
3. Substrate is converted to product
4. Enzyme is converted to product

45. Cholesterol is a precursor for each of the following except-

1. Bile salt
2. Vitamin D
3. Insulin
4. Steroids

46. How many water molecules will be released if four amino acids will be joined linearly
neglecting proline in the chain?

1. 3
2. 4
 3. 2
4. 1

47. The most common secondary structure of protein is -

1. Beta-pleated sheet
2. Alpha Helix
3. Beta pleated sheet parallel
4. Beta pleated sheet Non-Parallel

48. A high content of triglycerides are found in -

1. LDL
2. HDL
3. VLDL
4. Chylomicrons

49. The fastest enzyme from the followings is-

1. DNA Gyrase
2. Pepsin
3. DNA Polymerase
4. Carbonic anhydrase

50. A protein having both structural and enzymatic traits is

(a) Collagen

(b) Trypsin
(c) Myosin

(d) Actin

B. Multiple Select Question : [M.S.Q] : (51 to 60)

51. Suppose you are a biologist who is studying the mechanism of protein formation of proteins
in biological systems. Then what will be your observations?

Choose the correct options

a. It is a substitution type of reaction.

b. Elimination of water molecules takes place while formation of the protein from the amino
acid.

c. Peptide bonds are formed during this event.

d. Phosphodiester bond is formed while this event.

52. Fatty acids are the building blocks of the fat in our bodies and in the food we eat. Some of
the facts about fatty acid is given below.

Choose the correct options from below

a. Fatty acid may be unsaturated (with double bonds) or saturated without double bonds.

b. Upon complete hydrolysis of one molecule of triglyceride it will produce three molecules of
fatty acids.

c. Neural tissues contain fatty acid in its structures.

d. Fat and Oils are triglycerides.

53. Enzyme is a substance that acts as a catalyst in living organisms, regulating the rate at which
chemical reactions proceed. Choose the correct statements about the catalytic cycle of enzyme
action.

a. Enzyme alters its shape to get fit into the substrate

b. Transient state structure of the substrate formed during the enzymatic reaction is transient and

unstable

c. Enzyme changes its structure while the reaction

d. Substrate is converted to product.

54.Chose the incorrect statement

a. Dietary proteins are the source of non-essential amino acid

b. Cellulose, Inulin, Starch and Glycogen are homopolymer of glucose.

c. Lectin is considered a secondary metabolite.

d. Phosphodiester bonds are found in DNA.

55.Lipids is a biomacromolecule. Chose the correct options for lipids.

a. Lipids have molecular weight more than 10,000 Daltons.

b. It forms vesicles during disruption

c. Lipids are not strictly macromolecule.

d. It is hydrophobic in nature.
56. We use different oxidizing agents to convert the aldehydic group of glucose to carboxylic
group and form saccharic acid and gluconic acid. What are the reagents used?

a. HCN

b. Bromine, Water

c. Nitric Acid

d. H2O

57. A nonreducing sugar is a carbohydrate that is not oxidized by a weak oxidizing agent.
Identify non-reducing sugars from the given options.

a) Sucrose

b) Glucose

c) Raffinose

d)Fructose

58.The three-dimensional structure of protein can be determined from the following methods.
Chose

the correct options

a) Spectroscopy

b) X-ray Crystallography

c) NMR

d) Microscopy

59. Which of the following statements is not true for protein synthesis
(a) amino acids are directly recognised by m-RNA.

(b) the third base of codon is less specific.

(c) only one codon codes for an amino acid.

(d) every t-RNA has more than one amino acid attachment.

60.Which among the following is correct about polysaccharides?

a) Cellulose can’t be digested by mammals unless they contain microbes that digest cellulose in
their

gut

b) Polysaccharides mainly act as the food storage or structural materials

c) Starch comprises of two forms, namely, amylose (water insoluble) and amylopectin (water

soluble)

d) Amylopectin has α-1, 4-linkage and 1, 6-glycosidic linkage

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Thank You,

Prepared By- Sehasree Mohanta : )