STRUCTURE OF SucROSE: ofucrase u+A

ChoH 3 Hgdolusis dil-aiods 91Ucs

CtboH ZYuimolaH mintuie Of D Gtlurose and D Fuetose
OH
These indicats Ra ACYDse ks made
OH Hoy OH
Ho OH Y'Cl2oH O
Unlt O each of Hese monasaa hasides
OH OH HOHC
OH A) Sucorse does not Aeduce tollens Heagan
B-Dfctofyronos
puouyronase OH
Chot hling Heargunt and does not
OSa)e
enht

Sucorase olongd boiling h e gucosoone

Joned due fo

hycbuciysis ofSucrase dozs not fom metfy! 9yeosid

and
moleculay wcight deturinci does not Undgo Muteotafon
Elemertal doalysis
Shouus Re molecwa 2o
omula af Sucmar IS-12 H, AU there observafons indicak +Aad he aylic Jorns

Joinel
fogtho Rrough
fuetes ae
) SucYasereacts
acetic dnhudiide 9 re Of gucase and
Re Canbonyt rouhs
a hoints hoe
of Rodium acete 9ivs
ucrase Ocataacetate gycosidie iokog
i CI in Giluos
otReruise become availalale ruetcsc
wld
Ch0H

5) SucTose eat utA dimetRyl Solthakinipianet

Ao ine
HO O
CH2Ac o Xlkoline Boluion fo on O ctamethyl8uorase
dhich

+CH3-C-o -c-C3 on hycboysis gives

46 tthrametfhyl D-6ucohuranost
CAC
HO O D- fructafuranase
and I 3,46 tchrametfiy
K Hlch Ll--oNa AccH NaoH Ga Hiy Og (oc),
H
C2HO (oHJg + (CHa)3s°
OAC

3ucose CH2OCH3
Sucrose Ocfaareta H

hydbronyl
tRis 9ueoctiorm

grouhs l
indicats

uorDse molecule.
Kem OCH3
'oH

Siate Rucrose is Stable Comkound indicatea Re Glucose io Suorase has a

hurranose
- 3 foH) groop This
i musd be csnd On each Catbon am om and ructose Uot +AeUranoseorm
 maltase an engme
6 Sueras is hydroluseod bby to2OC
Efeet of Heat when Rucrose is heattd
1)
+Ras hydrolyses glycasids it fornsa brown mass
Coutame l Ohih

Also hydolysed byiovertas -an nbym chasactri sttes lowouw

because Color and

B-bu ot d- frutoguTanasid
hycdolySes n is Used a Colouxing and
bavowing ognt in

These observations indicat +Rat guccOYDS

foods and Condics
and P-fructoside
is bot an gucaside chans 7o almos1

A+ high temhratuwe ucorase

Coslbon and 91ves Vahow of co, Co, CHa

+RP above widence cde a ChoH huwre

thefollOwing St fonic aid, aceic aid

3ure Cylene acctylene, acetonc

be nottd +Ras the HO DH etional and acrolcin

+snouis
in Sucrose has +Re
ructose Unif
itA Conc. HNO3
but when SuorDs Onidaton omidatom of Sucros
ranas fm HD lin aud,
a miatr Omalie aud, tartauic
is the hyranose
yi d d s
of
huddgsed D-GuucaHic acid

fructase hich
obtaincd CH20
Jom CoOH
cOOH
CooH
a9 + o
(CHoH)4 CoOH
HOH)
Prohotie CooH
Onalic
CoOH

D-6uurasic add tattic

dud
aud
Cololss, Odcuw les s ystalln
Physical SuorDK is
2olid mp 185c tis
20uble in w atur and Reduchion:Reduction f Sucras uItR 8odium&ophydic

An oqe0us 8outon or Sodium amalgam waler unde Controlled Coodiions

inalcohol
alig r 2ouble
D- nmannitol

and does not hibit mutarnk D Srbital and

Sucose s dentorotuto

chenicol Sucmse i9 non Aeduinq Zuga Ctl20H

CHOH

Tollens, rhling nesg No-Hg +HO-C-H

not yadigo Aeact
(CHOH)
ydonykamin (or) hhenyehydrarynt CH20H Ho-
Hydxoge yanide --OH
DSorbitol
How H vs the followinq ncacions

ChoH

D- man itol
 igvertaSe) ) Hegdbrolug is
A)Hycbolys (In vert Suga (ov) Thise
aud 9ive
Cornhounds Aeoclily dissolve Lohen CO2
dilut
witA hot
of Sucrose hassed to
Ogeou Rushensgion
Strootium Coonhound is Used for icolatin9 huwr
Ci2 taa O + H0
rucrose rom non- nystalligable
Sucrase Glucose ctose molasss

+66.5
l , +53
Puers
C2Haa S(oH), C0
Invert Suu Surose lo -Ho
Strontiun Suorasaht STCO3
: (ts3+C4i): ~39 molacs

Sucras a dtmrorotextory o) f66.S 6 Reactton itA Acetic Anhudide Acety lation

is also dentroutartory t 5 3 +53 bu D-fuctus Acetic Anhudsuicle in r e n o

has Sucros eacs uitA
Roxg negaive otatin b Since D uatote of SodiUm acctak to giv ucrase octa aceteate
ha a
greater shecifte tofatton +Ran D Otucos
The Aesultig miMtr i3 levorotatovy

Becau of tRt he hycdolySiS 0f Sucrage +CHC-0 -i-CHa

is Roown a loversion oSucrase+Re cqulmolan CH CoNa

mimtr of D-Gtluros and DCctose

Lnvurt Suga () DvertaSe

Ferntatton
The iqvorsion (te hy oluolysis) of Sucmos Co
also be elene brough bouw Aa oeous 8oluion of uoros Headily
a
by t neym
Calleo iqvortase gound Fermentrd by yeast to giv etRanol and CO
yeast
iovurtase teaent in +Ae east
The enymn
5 ReacHo metelle hydoides Fomation o1 Suurosa
diYst Convevts SucoYS roto ucas and ructos

SucraS i9
aeous Zolution Heat u i
hyduo aides of
Thae Sugas Ren decomhosed by He enyc
Bay ium, Strontium anol Coleium
Zymaax qlso esunt n yeast to giv etu aduoho
oLoduce nsoluble Comhounds
Called Sucrosafts
(CHaCuo)