Name: GWENDOLYN SHELBY A.

CALATRAVA, BSED-BIO3 Section: W56

Schedule: Wed 1:30pm-4:30pm Date: April 20, 2022

Laboratory Task 1

A. Microbiology Laboratory Equipment/Tools

SIGN/SYMBOL DEFINITION

Disinfect work areas before and after use.

Use a disinfectant, such as a 10% bleach or 70%

ethanol solution, to wipe down benches and work
areas both before and after working with cultures.

Wear gloves when working with cultures.

Wear gloves when working with cultures, and when

your work is completed, dispose of the gloves in the
biohazard garbage. Lab coats, safety glasses or
goggles are also required. These will be stored in the
laboratory each week in a ziplock bag.

Properly sterilize.

Cultures of live microorganisms and any material

coming in contact with live cultures must be properly
sterilized after use in the laboratory. Your instructor will
inform you of specific procedures.

Do not eat or drink in the lab, nor store food

in areas where microorganisms are stored.

Never eat or drink in the laboratory while working with

microorganisms. Keep your fingers out of your mouth,
and wash your hands before and after the laboratory
activity. Cover any cuts on your hands with a
bandage. Gloves may be worn as extra protection..

Know where specific safety equipment is

located.

Know where specific safety equipment is located in

the laboratory, such as the fire extinguisher, safety
shower, and the eyewash station.

Wash your hands.

Use a disinfectant soap to wash your hands before
and after working with microorganisms.
Nondisinfectant soap will remove surface bacteria and
can be used if disinfectant soap is not available.
Gloves may be worn as extra protection.
 Clean up spills with care.
Cover any spills or broken culture tubes with a 70%
ethanol or 10% bleach solution; then cover with paper
towels. After allowing the spill to sit with the
disinfectant for a short time, carefully clean up and
place the materials in a biohazard autoclave bag to be
autoclaved. Wash the area again with disinfectant.
Never pick up glass fragments with your fingers or
stick your fingers into the culture itself; instead, use a
brush and dustpan. If working with animal or plant
pathogens, keep the area clear and notify your
instructor.

Label everything clearly.

All cultures, chemicals, disinfectant, and media should

be clearly and securely labeled with their names and
dates. If they are hazardous, label them with proper
warning and hazardous information.

Never pipette by mouth.

Use pipette bulbs or pipetting devices for the

aspiration and dispensing of liquid cultures.

Notify your instructor about the spill.

If the chemical is marked "danger" or "caustic" you

should notify the instructor who will handle this type of
spill.

Make sure that lab benches are

completely cleared.

Make sure that lab benches are completely cleared

(everything either thrown away or returned to storage
area) before you leave the lab.

Wash Laboratory Clothes.

Clothing worn in the microbiology laboratory should

be washed before being subsequently worn in a
facility such as a hospital, clinic or nursing home, or in
an area of public food preparation.
 Never place contaminated material on
the bench top.

Sterilize loops before and after each use. Place

contaminated pipette tips in the orange biohazard
buckets on your bench. Place all other contaminated
materials in their designated waste containers. Do not
place or put anything containing live microorganisms
in the sink.

No pets are allowed in the laboratory.

Leave all laboratory facilities and

equipment in good order at the end of
each class.
Before leaving the laboratory, check to make sure the
bacticinerator heat sterilizer is turned off.

Long hair should be pulled back.

Long hair should be pulled back to keep it away from

bacterial cultures, bacticinerator or open flames.

Recognize the international symbol for

biohazards.

Recognize the international symbol for biohazards,

and know where and how to dispose of all waste
materials, particularly biohazard waste. Note that all
biohazard waste must be sterilized by autoclave
before it can be included in the waste stream

Wear close-toed shoes.

You must wear close-toed shoes while in the

laboratory and long pants.

Follow precautionary statements given

in each exercise.
Always keep cultures capped and in
proper storage racks when not being
used during an exercise.