BRENT HOSPITAL & COLLEGES INC.

College of Medical Laboratory Science

Hematology 2 Lab.

Name: Date Performed: 02-28-2023_______

Crs/Yr: BSMT III-A Date Submitted: 01--2023_______

Activity # 02
Exam. of Platelets in Stained Smear

I – Objective(s):

II – Materials:
70% Alcohol Glass slide Microscope
Cotton balls Spreader Tissue paper/gauze
Lancet Wright’s stain Forceps
Tally counter Cedar wood oil

III – Procedure:
1. Obtain blood by skin puncture.
2. Make a smear & allow to dry.
3. Stain the smear with Wright’s stain & allow to dry.
4. Examine the stained smear under the microscope. Focus under the LPO & then
shift to HPO.
5. Observe the appearance of platelets as seen under the HPO. Record your
observations.
6. Count platelets in 10 fields under HPO & compute as follows:

Platelet/ct.mm3 = Plt ctd x 2000

IV – Observation & Drawing

Platelet Count 1st field .
2nd .
3 rd
.
4th
5th
TOTAL=
Computation: Platelet/ct.mm3 = Plt ctd x 2000

Name of Patient:
 Result:
Normal Value:
Interpretation:

V – Conclusion: