Selective for Neisseria

gonorrhoeae and Neisseria

meningitidis
Modified Thayer martin(TM)
Selective enrichment medium for
recovery of Neisseria gonorrhoeae
and Neisseria meningitidis

Incubate in a increased CO2

Modified Thayer martin(TM)

Contains (VCNT)
V=ancomycin: inhibits the gram-positive bacteria
C=olistin: Inhibits the gram-negative bacteria
N= ystatin: inihibits the growth of fungi
T=rimethoprim: inihibits swarming of proteus
species
Thayer martin

Contains (VCNT)
V=ancomycin: inhibits the gram-positive bacteria
C=olistin: Inhibits the gram-negative bacteria
N= ystatin: inihibits the growth of fungi
Martin-lewis

Selective enrichment medium for recovery of N.

gonorrhoeae and N. meningitidis from specimen
with normal flora.

Inhibits yeast better than TM

Martin-lewis
Contains (VCAT)
V= ancomycin: inhibits gram-positive bacteria
C=olistin: Inhibits gram-negative bacteria
A=nisomysin: inhibits yeasts
T=rimethoprim: hinders the growth of proteus
New York City agar
• Selective enrichment medium for isolation of N.
gonorrhoeae and N. meningitidis from specimen with
normal flora
• Genital mycoplasma will also grow
New York City agar
• Contains (VCAbT)
• V=ancomycin
• C=olistin
• Ab=amphotericin B- inhibits most of the fungi
• T=rimethoprim
GC-LECT agar
• Is a selective plated medium that provides enhanced
growth of N. gonorrhoeae.
• Provides additional inhibition required to prevent the
overgrowth of the pathogenic variety of Neisseria in
specimen containing capnocytophaga
JEMBEC PLATES
• For transportation and growth of N. gonorrhoeae
• Contains Neisseria selective medium and come with
resealable polyethylene bag and CO2 generating
tablet.
• No need to transfer to culture plate
PLATING MEDIA FOR ANAEROBE BACTERIA
Bacteriodes bile-esculin agar

• Selective differential medium for

isolation and identification of
bacteroides fragilis
• Bile salts and gentamicin act as
inhibitors
• Hydrolysis of esculin indicated by
blackening of agar
CDC Anaerobic Blood Agar
• Is an enrichment medium for isolation of fastidious
anaerobes
• Contains yeast extract, L-cysteine, hemin, and
vitamin K.
Cycloserine cefoxitin fructose egg yolk
agar(CCFA)
• Selective medium for Clostridium difficile
Cooked meat medium

• For isolation of anaerobes, especially pathogenic

clostridium
Egg-yolk agar(EYA)

• For determination of
lecithinase and lipase
production by some
clostridia
Laked kanamycin-vancomycin blood agar
• Selective medium for bacteroides and prevotella
Phenylethyl alcohol agar(PEA)
• Inhibits enteric gram-negative rods and swarming by
some clostridia
Thioglycollate broth(THIO)
• All purpose medium that supports the growth of most
aerobes and anaerobes. Can be used as a backup
broth to detect organisms present in small numbers or
anaerobes.
Thioglycollate broth(THIO)

• Thiogylcolate acts as a reducing agent. Aerobes grow

at top, strict anaerobes at bottom, facultative
anaerobes throughout
• Store at room temperature and boil and cool prior to
use
SPECIAL BACTERIOLOGIC MEDIA
Cystine-tellurite blood agar
• Differential medium for isolation of
Corynebacterium diphtheria
• C. diphtheria produces a black
colonies
LOEFFLER MEDIUM
• Enrichment medium for
recovery and identification
of Corynebacterium
diphtheria
• Promotes development of
characteristic granules
Tindale agar
• Selective differential medium for
Corynebacterium diphtheria
• Corynebacterium spp. produce gray
to black colonies due to reduction of
tellurite.
• C. diphtheria colonies are surrounded
by a brown halo.
Cefsulodin-irgasan-novobiocin agar(CIN)
• Selective medium for Yersinia
enterocolitica, Aeormonas and
Plesiomonas shigelloides
• Crystal violet inhibits most gram-
negatives.
• Novobiocin inhibits gram positive
cocci
Cefsulodin-irgasan-novobiocin agar(CIN)
• Cefsulodin inhibits most gram-positive and gram-
negative
• Yersinia enterocolitica ferments mannitol and
appears as red bull’s-eye colonies surrounded by
colorless halo.
 Bismuth sulfite agar
• Selective medium for isolation of
Salmonella
• Bismuth sulfite and brilliant green inhibits
most others
• Salmonella typhi produces a black colonies
surrounded by a metallic sheen
• Others are light green.
Alkaline peptone water
• Enrichment medium for recovery of vibrio and
Aeromonas from stool
• Alkaline pH suppresses commensals.
• Subcultured to TCBS
Thiosulfate citrate bile salts sucrose
• Best medium for isolating vibrio
• High pH inhibits most bacteria
• V. cholerae ferments sucrose and produces yellow
colonies
• V. parahaemolyticus and V. vulnificus don’t ferment
sucrose and usually produce a blue-green colonies
Rabbit Blood agar
• Used to speciate haemophilus
• Haemophillus haemolyticus and Haemophillus
parahemolyticus are hemolytic
 Bordet-gengou
• Selective enrichment medium for isolation of
Bordetella pertussis
• Potato-glycerol based medium enriched with blood
• Methicilin: inhibits contaminants
• Cough plate
• Bordetella colonies resemble mercury droplets
Bordet-gengou
Regan lowe
• Selectivfe for Bordetella pertussis
• Chocolate agar supplemented with horse blood,
cephalexin and amphotericin B
Buffered charcoal yeast extract agar(BYCE)
• Enrichment medium for isolation of
leigionella
• Yeast extract and L-cysteine enhance
growth of legionella.
• Charcoal absorbs toxic compounds
 Vaginalis agar plate(V agar)
• Nonselective enrichment medium for isolation of
garnerllavaginalis
• Columbia agar with human blood. Incubated in
increased CO2 for 24-72 hours
• Gardnerella vaginalis produces fishy odors and
itchiness in the person infected
• G,vaginalis produces diffuses beta hemolysis
Human blood Tween agar(HBT)
• Semi-selective medium for G. vaginalis
• Incubate in an increased CO2 for 48 hours. Colonies show diffuses
beta hemolysis
Lowenstein jensen
• Selective enrichment medium used to culture mycobacteria
• Contains potato flour, egg and glycerol
• Malachite green inhibits other bacteria
• Mycobacterium produces a rough and buff or cauliflower like colonies
Middlebrook 7H10 and 7H11
• Selective enrichment medium used to culture mycobacteria
• Agar based.
• Isoniazid-resistant strains grow better than an egg-based media.
• Contains malachite green to inhibits other bacteria
Fletcher medium
• Enrichment medium for isolation of leptospira from blood, CSF and
urine
• Contains rabbit serum