SMP IBC IMMANUEL

JL.SUTOYO 1
LAB PRACTICE
PONTIANAK
SCHOOL YEAR 2013-2014

Food Test Exercise[Amilum,Glucose and protein]

A.Objective
-Students can use different solutions in order to test the Existence of certain nutrition.
-Students can learn to decide the types of food which contains the vitamins and
minerals which body needs

B.Tools and Materials

-Reaction tube and reaction tube rack
-reaction tube tool
-Dropping plate
-spiritus burner
-dropping pipette
-lugol solutions
-benedict solutions
-biuret reactor
- fehling A and fehling B solutions
-Food-stuff(rice,banana,peanut,tomato,egg)

C.Experiment steps
Experiment 1(Amilum Test)
1.every food materials have to be crushed into powder,and placed into a container and
give labels for each container
2.give each food materials with lugol solutions for about 2-3 drops
3.note the colour exchange in the table.
Experiment 2(protein tests)
1.put each of the food materials in the basin of the dropping plate.label it with A to E
According to the food samples
2.drop it with the biuret solutions(NaOH and CuSO4).
3.note the colour changing in the table.
Experiment 3(sugar reducing test:glucose)
1.put each of the materials into the reaction tube,add some water.and label with A
Until E,according to each food container
2.drop each food samples with fehling A and fehling B about 4 drops
3.using the reaction tube tool each tube must be heat above the spirit burner.
4.note the colour changes in the table
Experiment 4( fat content test)
Apply the food samples to the swabs oil paper.note the changes happen.
 2

D.observation result

Table:food test result

NO Food Reaction/colour changes Food test results
samples Fehling AB Lugol biuret Oil paper glucose amil proteins fats
um
1 rice Blue green Blue black purple White + - + -
transparent
2 banana Yellow brown Yellow brown purple No changes - - + -
3 Tempe brown Yellow brown purple No changes - + + +
4 tomato Yellow red orange blue No changes - + - -
5 peanuts brown brown purple No changes + + + +

E.conclusion:
1.what are the names of experiment uses to test:
a.powder solutions:iodine test
b.glucose:benedict test
C.proteins:biuret test
2.according to the test result,what kind of food contains:
a.Amilum:rice
b.proteins:banana,peanut,rice,tempe
c.glucose:tomato,rice,banana
3.Amilum(flour substance) and sugar are carbohydrates.what are their differences?
4.what are each of the functions of carbohydrates and proteins for our body?
Carbohydrates provides energy for our daily activity,protein help our body growth and repair
our body cells
5.what are the simplest form of:
a.Amilum:glucose
b.proteins:Amino acids

6.how to test fats existence in the food?

By doingexperiment 4,applying the facial oil paper to the food samples
7.show the disease shown by the lack if these nutritions:
a.protein:kwasiorkhor diseases
b.glucose:will cause the body become thinner and weaker