Antibiotic "#13 ar Di l usion Techniqu Susceptibility testing to differ 1 timicrobial therapy, specifically in nied timicrobiat agents is very important for instituting rational sere are many methods in the laborar, thee 26 not responding to antibiotic therapy, although towards the antibiotic, the Kirby Bauer ae aioe determine the susceptibility of the isolated bacteria eficacy and simplicity. In this technigu 30? technique remains the method of choice due to its le, fuse n the Mueller-Hinton agar (MHA) thorn 2" amount of antibiotic in the paper disc is allowed to ‘ that hasbeen swabbed with the standerd amount of inseam cells/ml). The susceptibi Me ete abisHS acre os bacteria 's determined by measuring the zone of inhibition incubati Aon te ainda Cree eee rethe sal yond phone eg ees st. These are the sur i Eat suital inoeulums, thickness of the agar, diffusion of the srunen in the disc, time and incubation temperature. Learning Outcomes: At the end of the exercise, the student is expected to: 1. Classify and describe the different types of antibiotics. 2. Perform with utmost accuracy the kirby-Bauer disc diffusion technique. 3. Interpret correctly the results of Kirby-Bauer disc diffusion technique. 4 Evaluate the antibiotics that were utilized in this exercise according to its efficacy against certain microorganisms. Petri plate containing Mueller Hinton Agar 0.5 McFarland Standard (0.5 ml 0.048M BaCl + 99.5 ml 0.18M H,SO,) Sterile cotton swabs Antibiotic discs Forceps Alcohol Vernier Caliper Alcohol lamp Tryptic Soy Broth or NSS Procedure: iq 1. Prepare inoculum from isolated colonies. 5 ion Method ernpirect en E aanared by making a direct broth or saline suspension of isolated i. Inoculum is r plate. lonies from 18 — 24 hour 263! a nets e suspension using a Sveti Standard. Met! b. Growth Method or eee colonies of the same morphological characteristics from a i Select 3-5 we plated agar. 1 2 a. 4 5B. 6. 7 & 9