Polimorphic Marker Untuk Identifikasi Keragaman Genetik Kelapa Sawi1 Menggunakan Rapd

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POLIMORPHIC MARKER UNTUK IDENTIFIKASI KERAGAMAN GENETIK

KELAPA SAWIT (Elaies guineensis Jacq.) DENGAN MENGGUNAKAN


RANDOM AMPLIFIED POLIMORPHIC DNA (RAPD)

Arnen Pasaribu1), dan Lollie Agustina P.Putri2)


1)
Mahasiswa Program Studi Agroekoteknologi, Fakultas Pertanian Universitas Sumatera Utara,
Jalan Prof. A. Sofyan No.3, Medan 20155
2)
Dosen Program Studi Agroekoteknologi Fakultas Pertanian Universitas Sumatera Utara, Jalan
Prof. A. Sofyan No.3, Medan 20155
Corresponding author : pasaribu30@gmail.com

ABSTRACT

Identification of variation genetic is the first step to find how much genetic variation contained
in a genotype. Now, the genetic material of palm oil has an inbreeding depression so the
genetic identification in moleculer level is important. The use of RAPD can be used as an
alternative to identifiying the moleculer level diversity. The main problem at the moleculer level
identification are primers was used are not compatibel with the composition of the target base
nucleotide. The objective of this experiment was to find genetic variation in palm oil
(E. guineensi Jacq.) by using three RAPD primers. Results of this experiment only two
informative RAPD primers to show genetic variation.

Keywords : Palm oil, Genetic Variation, RAPD

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