Chapters 4,5

Cellular
Energy
Todays Topics
Producers Consumers
Plants Animals
Alga Most Bacteria
Some Bacteria fungi
Producers and Consumers in the Carbon
Cycle
 Producers, Consumers and Decomposers
(Bioenergetics)

How do animals and plants

process energy?

Most life forms get their energy from the sun.

Plants use photosynthesis to capture sunlight,
and herbivores eat the plants to obtain energy.
Carnivores eat the herbivores, and eventual
decomposition of plant and animal material
contributes to the nutrient pool
 Metabolic Pathways
Consider the metabolism of sugar. This is a classic example of one of the many cellular
processes that use and produce energy.

Living things consume sugars as a major energy source, because sugar molecules have a
great deal of energy stored within their bonds. For the most part, photosynthesizing
organisms like plants produce these sugars.

During photosynthesis, plants use energy (originally from sunlight) to convert carbon
dioxide gas (CO2) into sugar molecules (like glucose: C6H12O6). They consume carbon
dioxide and produce oxygen as a waste product. This reaction is summarized as:
 Metabolic Pathways
Because this process involves synthesizing an energy-storing molecule, it requires energy
input to proceed. During the light reactions of photosynthesis, energy is provided by a
molecule called adenosine triphosphate (ATP), which is the primary energy currency of all
cells.

The reaction that harvests the energy of a sugar molecule in cells requiring oxygen to survive
can be summarized by the reverse reaction to photosynthesis. In this reaction, oxygen is
consumed and carbon dioxide is released as a waste product.
 Metabolic Pathways
Metabolic pathway: A series of chemical reactions that
takes a starting molecule and modifies it, step-by-step,
through a series of metabolic intermediates, eventually
yielding a final product.
Linear vs Cyclic Metabolic Pathways
Metabolic Pathways
Adenosine Tri Phosphate (ATP)
Types of Energy
Energy
Chemical Reactions
Getting things going…
Activation Energy
 Enzymes
Some chemical reactions are too slow or have activation energy requirements that are
too high to make the functionally practical
These chemical reactions are made possible by catalysts

Catalyst: Substance that speeds up he rate of chemical reactions

Does so by lowing the reactions activation energy
Enzymes: Biological catalysts that speed up reactions, very specific
Substrate: Reactants of enzyme catalyzed reactions
Active Site: Domain of the enzyme where substrate binds
Enzyme name ends with “-ase” and starts with it’s substrate
Activation with an Enzyme
Enzyme Regulation = Metabolic Pathway
Regulation
Since the rates of biochemical reactions are controlled by activation energy, and
enzymes lower and determine activation energies for chemical reactions, the relative
amounts and functioning of the variety of enzymes within a cell ultimately determine
which reactions will proceed and at what rates.
This determination is tightly controlled in cells. In certain cellular environments,
enzyme activity is partly controlled by environmental factors like pH, temperature,
salt concentration, and, in some cases, cofactors or coenzymes.
 Competitive Inhibition
Enzymes can also be regulated in ways that either promote or reduce enzyme activity.
There are many kinds of molecules that inhibit or promote enzyme function, and
various mechanisms by which they do so. In some cases of enzyme inhibition, an
inhibitor molecule is similar enough to a substrate that it can bind to the active site
and simply block the substrate from binding. When this happens, the enzyme is
inhibited through competitive inhibition, because an inhibitor molecule competes
with the substrate for binding to the active site.
 Non Competitive Inhibition
Noncompetitive inhibition, an inhibitor molecule binds to the enzyme in a location
other than the active site, called an allosteric site, but still manages to block substrate
binding to the active site. Some inhibitor molecules bind to enzymes in a location
where their binding induces a conformational change that reduces the affinity of the
enzyme for its substrate.
 Non Competitive Inhibition
Allosteric Regulation: secondary molecule interacts
with the enzyme to either promote or inhibit substrate
binding
Feedback Inhibition = Metabolic Pathway
Regulation
Starting molecules and products of the pathway can act as regulatory molecules of
enzymatic cellular metabolism.
Feedback Inhibition: The cell responds to an abundance of the products by slowing
down
Disorders Associated With Defects in
Metabolic Processes
Disorders Associated With Defects in
Metabolic Processes
Disorders Associated With Defects in
Metabolic Processes
Glycolosis
ATP (How much ATP is in the human body?)
Glycolysis Requires ATP
Extra Video Available
Cellular Respiration
 Glycolysis
• Glycolysis is the first step in the breakdown of glucose
to extract energy for cell metabolism. Many living
organisms carry out glycolysis as part of their
metabolism.
• Glycolysis takes place in the cytoplasm of most
prokaryotic and all eukaryotic cells.
• Begins with the six-carbon, ring-shaped structure of a
single glucose molecule and ends with two molecules
of a three-carbon sugar called pyruvate.
Glycolysis consists of two distinct phases
1. Energy is used to make adjustments so that
the six-carbon sugar molecule can be split
evenly into two three-carbon pyruvate
molecules.
2. In the second part of glycolysis, ATP and
nicotinamide-adenine dinucleotide (NADH)
are produced
• If the cell cannot catabolize the pyruvate molecules
further, it will harvest only two ATP molecules from one
molecule of glucose. For example, mature mammalian
red blood cells are only capable of glycolysis, which is
their sole source of ATP. If glycolysis is interrupted,
these cells would eventually die.
 Citric Acid/Krebs Cycle
The citric acid cycle is a series of enzyme-catalyzed
chemical reactions
Citric Acid Cycle
 Oxidative Phosphorylaion
Process that begins with passing electrons through a series of chemical reactions to a
final electron acceptor, oxygen.
These reactions take place in specialized protein complexes located in the inner
membrane of the mitochondria of eukaryotic organisms and on the inner part of the
cell membrane of prokaryotic organisms.
The energy of the electrons is harvested and used to generate a electrochemical
gradient across the inner mitochondrial membrane. The potential energy of this
gradient is used to generate ATP. The entirety of this process is called oxidative
phosphorylation.
Electron Transport Chain
ATP Synthase
ATP Synthase

The accumulation of protons in the

intermembrane space creates an
electrochemical gradient that causes protons
to flow down the gradient and back into the
matrix through ATP synthase. This movement
of protons provides the energy for the
production of ATP.

The electron transport chain is the third step of

aerobic cellular respiration. Glycolysis and the
Krebs cycle are the first two steps of cellular
respiration.
Cellular Respiration
Anaerobic Respiration
Fermentation
Anaerobic Respiration in Animal Cells
Lactic Acid Fermentation
Lactic Acid Fermentation in Muscles
 Alcohol Fermentation in Yeast

The first reaction, a carboxyl group is removed from pyruvic acid, releasing carbon dioxide as
a gas. The loss of carbon dioxide reduces the molecule by one carbon atom, making
acetaldehyde.

The second reaction removes an electron from NADH, forming NAD+ and producing ethanol
from the acetaldehyde, which accepts the electron.

The fermentation of pyruvic acid by yeast produces the ethanol found in alcoholic beverages
Photosynthesis
Comparing Metabolic Processes
Photosynthesis
• The location and structure of chloroplasts
Chloroplast
LEAF CROSS SECTION MESOPHYLL CELL
LEAF

Mesophyll

CHLOROPLAST Intermembrane space

Outer
membrane

Granum Inner
membrane
Grana Stroma Thylakoid
Stroma Thylakoid compartment
Chloroplast cont.
 Light

Light is a form of energy with different wavelengths

The shorter the wavelength, the greater the energy of each photon of light
Certain wavelengths of light are detectable by human eyes and seen as colours

Visible light drives photosynthesis

Why are plants green?
Plant Cells
have Green
Chloroplasts

The thylakoid
membrane of the
chloroplast is
impregnated with
photosynthetic
pigments (i.e.,
chlorophylls,
carotenoids).
 Why are plants green?
• Chloroplasts absorb
light energy and
convert it to
chemical energy
Reflected
Light light

Absorbed
light

Transmitted Chloroplast
light
 Chloroplast Pigments
• Chloroplasts contain several pigments
– Chlorophyll a
– Chlorophyll b
– Carotenoids
– Xanthophyll

Figure 7.7
 Two stages of photosynthesis

• A summary of the
chemical processes Chloroplast

of photosynthesis Light

Photosystem II
Electron
transport CALVIN
chains CYCLE Stroma
Photosystem I

Cellular
respiration
Cellulose
Starch
Other
LIGHT REACTIONS CALVIN CYCLE organic
compounds
 Photosynthesis (visual overview)
• The light reactions
Light
convert solar energy Chloroplast
to chemical energy
• Produce ATP & NADPH NADP+
ADP
+P
Calvin
• The Calvin cycle makes Light
reactions
cycle

sugar from carbon

dioxide
– ATP generated by the light
reactions provides the energy
for sugar synthesis
– The NADPH produced by the
light reactions provides the
electrons for the reduction of
carbon dioxide to glucose
Three parts to light reaction
Photosystems
Photosystems
Photosystems
Movement Through Photosystems
Calvin Cycle – dark reaction
Calvin Cycle
Calvin Cycle
Photosynthesis Reactions
Producers and Consumers in the Carbon
Cycle
Photosynthesis and Cellular Respiration