NAME: Adil, Bernadette T.

Date: 10-20-22 SCORE:__________

Lab. teacher: Aurea Tolentino-Coros Course & Section BSN - 1 C Grp. No.: 2

ACTIVITY NO. 6
COLOR REACTION OF PROTEINS
Results:

Test Albumin Gelatine

Purple color Purple color

Biuret

Purple/blue color Purple/blue color

Ninhydrin

Xanthoproteic Yellow color with white Faint yellowish to colorless color

a.With HNO3 precipitate (+) (+)
Yellowish color with white Yellowish color (+)
b. with NH4OH precipitate (+)
Red precipitate is formed Creates a pinkish red solution
Millon’s

Forms a purple ring No ring was formed

Hopkin’s-Cole

No precipitate formed (-)

Lead Acetate Black precipitate formed (+)
Cherry red color (+) Cherry red (+)
Pauly

Colorless/white color Orange color

Bromine Water

Red color
Sakaguchi Red color
 NAME: Adil, Bernadette T. Date: 10-20-22 SCORE:__________
Lab. teacher: Aurea S. Tolentino-Coros Course & Section: BSN – 1C Grp. No.2

ACTIVITY NO. 6
COLOR REACTION OF PROTEINS

Question Guide:
1. Write the positive result, the chemical group responsible for the positive and the
importance of each test.

Test Positive Result Chemical Group Importance/Significance

deep blue/purple Amino group it determines the presence of the

Biuret color peptide bonds present in a
substance

red/blue/purple Amine functional it detects the presence of amino

Ninhydrin color group acid that is present in an unknown
sample

Yellow or Aromatic group -This is a biochemical test for the

Orange color detection of proteins and amino
Xanthoprotei acids.
c
-The test allows the differentiation
of aromatic amino acids from non-
aromatic amino acids.

Red/pink Phenolic hydroxy It is used for the detection of

Millon’s precipitate group tyrosine-containing proteins in a
given sample

Purple colored Indole group It is one of the color reactions used

ring for the detection of particular
Hopkins-Cole amino acids or proteins on the
basis of the formation of a specific
color

Black Sulfhydryl/ thiol To determine whether a sample

Lead Acetate group contains sulfur-containing amino
acids.
 In order to detect cysteine-
containing proteins and cystine in
a sample.
To tell the difference between
sulfur-containing and sulfur-free
amino acids.

Red color Imidazole and -To detect the presence of tyrosine

hydroxyl group and histidine-containing proteins.
Pauly
-To differentiate between histidine
and tyrosine from other amino
acids.

Bromine colorless Alkene group used to determine any

Water solution alkene/alkane groups for
unsaturated carbon bonds

Sakaguchi Guanidine group to detect the presence of arginine

red color proteins
solution

2. What color will be produced by proline with Ninhydrin test? Why?

The color that will be developed by proline due to ninhydrin test is a yellow color, since it
contains a secondary amine group which is called an Imine. Proline is the only proteinogenic
secondary amino acid thus, the amino acids are oxidized by ninhydrin to produce the brightly
colored anion with the exception of both of the proline and hydroxyproline.

3. Which test can be used to show the extent of hydrolysis of proteins? Why?
The test that can be used is the Biuret test, since Biuret test can show the extent of hydrolysis of proteins
due to the coordination complex with the peptide bond of the protein from the CuSO4

4. Classify proteins according to nutritional value. To which classification does albumin and
gelatin belong?
Proteins are classified into three types based on their nutritional value: Complete proteins, partially
complete proteins, and Incomplete proteins. Complete proteins contain all essential amino acids
in sufficient quantity to meet the body’s requirements. This is derived from animals such as red
meat, milk, fish, and eggs, as a result albumin is classified as complete protein because it is
abundant in those food. Incomplete protein, on the other hand, lack one or more essential amino
acids and thus cannot support life on their own. Therefore gelatin is classified as incomplete
protein, that is, incapable of replacing or building new tissue and hence cannot support life. Plants
that contain these proteins are vegetables, grains, seeds, and nuts. Moreover, partially complete
proteins do not contain all nine essential amino acids, but rather only a small amount of one or
more of them.