1

MALT AGARS

Malt Extract Agar ME For the premixed malt agar: Use 25 g

H2O 500 mL
Agar 10 g
Malt 10 g

Malt-yeast Extract MYA

H2O 500 mL
Agar 10 mL
Malt 10 mL
Yeast 1 mL

Malt Extract with Activated MEAC

Carbon
H2O 500 mL
Agar 10 g
Malt 10 g
Activated Carbon 1g

Malt Extract with Peptone MEP

H2O 500 mL
Agar 10 g
Malt 10 g
Peptone 1g

Malt Extract with Yeast and MYAP

Peptone
H2O 500 mL
Agar 10 g
Malt 10 g
Yeast 1g
Peptone 1g

Malt Extract with Yeast, MEPYAC

Peptone, and Activated
Carbon
H2O 500 mL
Agar 10 g
Malt 10 g
Peptone 1g
Yeast 1g
Activated Carbon 1g
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Wheat Malt-yeast Extract WMYA

H2O 500 mL
Agar 10 mL
Wheat Malt 10 mL
Yeast 1 mL

Wheat Malt Extract with WMEP

Peptone
H2O 500 mL
Agar 10 g
Wheat Malt 10 g
Peptone 1g

Wheat Malt Extract with WMYAP

Yeast and Peptone
H2O 500 mL
Agar 10 g
Wheat Malt 10 g
Yeast 1g
Peptone 1g

Malt Extract with Milo MEM

H2O 500 mL
Agar 10 g
Malt 10 g
Ground Milo 10 g

Malt Extract with Corn MEC

H2O 500 mL
Agar 10 g
Malt 10 g
Ground Corn 10 g

Malt Extract with Honey MEH

H2O 500 mL
Agar 10 g
Malt 10 g
Honey 16 g
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ATTC Medium 597 597

H2O 500 mL
Agar 10 g
Malt 3.75 g
Yeast 0.3 g
Peptone 0.5 g

MYAP with Multi-vitamin MYMP Add Multi-vitamin after sterilization.

H2O 500 mL
Agar 10 g
Fresh ground crickets 8g
Multi-Vitamin 0.01 g
Peptone 1g
Yeast 1g
Malt 10 g

Can use just 1 or 2 elements, so label

MYAP with elements MYPMCZ
MYPZ or MYPCZ, etc.
H2O 500 mL
Agar 10 g
Malt 10 g
Yeast 1g
Peptone 1g
Calcium 0.01 g
Magnesium 0.01 g
Zinc 0.01 g

Yeast Malt Extract YME

H2O 500 mL
Pre-mixed Malt Agar 25 g
Yeast 1g

Peptone Malt Extract PME

H2O 500 mL
Pre-mixed Malt Agar 25 g
Peptone 1g

MYA with CS4 CS4MY

H2O 500 mL
Agar 10 g
Malt 3.75 g
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Yeast 0.3 g
Ground Cordyceps Sinensis 4 10 g

MYA with CSG2 G2MY

H2O 500 mL
Agar 10 g
Malt 3.75 g
Yeast 0.3 g
Ground Cordyceps Sinensis 10 g
G2

Chitin Method :ch Add 0.3 g chitin to any malt

agar

For 24 test tubes (1 rack) use:

432 mL H2O
8.6 g Agar
8.6 g nutrient
0.86 g yeast/peptone

ANIMAL RELATED MEDIA

Blood Worm Agar BW

H2O 500 mL
Agar 10 g
Blood Worms 5g

Krill Agar KA
H2O 500 mL
Agar 10 g
Krill 10 g

Fish Flake Dextrose Agar FDA

H2O 500 mL
Agar 10 g
Fish Flakes 3g
Dextrose 10 g
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Meal Worm Agar MW

H2O 500 mL
Agar 10 g
Meal Worms (ground) 10 g
Dextrose 3g
Yeast 1g

Fishy Wax Agar FWA

H2O 500 mL
Agar 10 g
Krill 3.3 g
Tubiflex Worms 3.3 g
Fish Flakes 3.3 g
Bee's Wax 1g

Cat Food Agar CFA

H2O 500 mL
Agar 10 g
Cat food 10 g

Dog Food Agar DFA

H2O 500 mL
Agar 10 g
Dog food 10 g

Dr. Pollock’s Modified Agar POLL

H2O 500 mL
Agar 10 g
Dog food 10 g
Dextrose or malt extract 2g

Difco Heart Infusion Agar DHA

H2O 500 mL
Agar 10 g
Beef Heart Infusion from 500g 10
Tryptose 5g
NaCl 2.5 g

Difco Charcoal Agar DCA

H2O 500 mL
Agar 10 g
Beef Heart Infusion from 500g 6g
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Peptone 5g
NaCl 2.5 g
Soluble startch (potato starch) 5g
Yeast 1.8 g
Norit SG 2g

Rabbit Food Agar RFA Boil the rabbit food. Steep for 30
H2O 500 mL minutes. Filter and add agar to
Agar 10 g
filtrate.
Rabbit food 12.5 g

Brown Rice Rabbit Food BRRF

H2O 500 mL
Agar 10 g
Brown Rice flour 10 g
Peptone 1g
Crushed Rabbit food 5g
Dextrose 1g

Bird Seed Malt Agar BSM

H2O 500 mL
Agar 10 g
Brown rice Flour 2.5 g
Ground Bird seed 5g
Malt 2.5 g
Peptone 0.5 g

Gold Fish Food Agar GFF

Agar 10 g
H2O 500 mL
Gold Fish Food 10 g

GRAIN/STARTCH MEDIA

Milo Agar MA
H2O 500 mL
Agar 10 g
Ground milo 10 g
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Milo Agar #2 MILO

H2O 500 mL
Agar 10 g
Ground milo 20 g
Yeast 1g

Modified Sabouraud's MSM

Medium
H2O 500 mL
Agar 10 g
Barley flour 25 g
Yeast 1g
Dextrose 5g
Neo-peptone (optional) 2g

Oatmeal Neo-peptone Agar ONA

H2O 500 mL
Agar 10 g
Oatmeal/oat flour 40 g
Neo-peptone (optional) 2g

Cornmeal Dextrose Agar CDA

H2O 500 mL
Agar 10 g
Cornmeal 25 g
Dextrose 3g

Barley Malt Extract Agar BMA

H2O 500 mL
Agar 10 g
Barley flour 40 g
Malt 2g
Yeast (optional) 1g

Teff Flour Malt Extract TFMA

Agar
H2O 500 mL
Agar 10 g
Teff flour 40 g
Malt 2g
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Yeast (optional) 1g

Garbanzo Bean Malt GBMA

Extract Agar
H2O 500 mL
Agar 10 g
Garbanzo bean flour 40 g
Malt 2g
Yeast (optional) 1g

Rice Bran Malt Extract RBMA

Agar
H2O 500 mL
Agar 10 g
Rice Bran 40 g
Malt 2g
Yeast (optional) 1g

Dark Rice Flour-Malt DRMA

Extract Agar
H2O 500 mL
Agar 10 g
Dark Rice flour 40 g
Malt 2g
Yeast (optional) 1g

Brown Rice flour-Malt BRMA

Extract Agar
H2O 500 mL
Agar 10 g
Brown rice flour 40 g
Malt 2g
Yeast (optional) 1g

Wheat Flour Malt Extract WFMA

Agar
H2O 500 mL
Agar 10 g
wheat flour 40 g
Malt 2g
Yeast (optional) 1g
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Oat Flour Malt Extract OFMA

Agar
H2O 500 mL
Agar 10 g
Oat flour 40 g
Malt 2g
Yeast (optional) 1g

Green Bean Powder Agar GBPA

H2O 500 mL
Agar 10 g
Green Bean Powder 10 g

ATCC Medium 336 336

H2O 500 mL
Agar 10 g
Diced Potatoes or 150 g or
Potato Starch 10 g
Dextrose 10 g

Avocado Peel dextrose Agar APDA

H2O 500 mL
Agar 10 g
Avocado Peels 10 g
Dextrose 7g

Potato, Ammonium citrate, PAT Cordyceps militaris known to

Thiamin Agar
fruit from this and brown rice in
H2O 400 mL
jars.
Agar 10 g
Potato Broth 100 mL
KH2PO4 1g
MgSO4 0.5 g
Peptone 1.5 g
Ammonium Citrate 0.5 g
Dextrose 15 g
Thiamine (B1 vitamin) 0.025 g
In the 500 mL of water boil the 150 g
of potatoes, and use this broth (not the
potatoes). Bring solution back to 500
mL with water then add remaining
ingredients. Potato flakes can be used
as a substitute (5 g in the 500 mL of
water).
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Potato Dextrose Agar PDA

H2O 500 mL
Agar 10 g
Dextrose or 7 g or
Honey/Corn syrup 10 mL
Diced potatoes 150 g

In the 500 mL of water boil the 150 g

Potato Dextrose Yeast Agar PDYA of potatoes, and use this broth (not the
H2O 500 mL potatoes). Bring solution back to 500
Agar 10 g mL with water then add remaining
Dextrose or 7 g or ingredients. Potato flakes can be used
Honey/Corn syrup 10 mL as a substitute (5 g in the 500 mL of
Diced potatoes 150 g water).
Yeast 1g

Potato Dextrose Agar PDA

H2O 500 mL
Agar 10 g
Diced and boiled Potatoes or 150 g or
Potato starch 10 g
Dextrose 7g

Potato Dextrose Agar PDY

H2O 500 mL
Agar 10 g
Diced and boiled Potatoes or 15 g or
Potato starch 10 g
Dextrose 7g
Yeast 1g

Potato Skin Dextrose Agar PSDA

H2O 500 mL
Agar 10 g
Dried Potato skins 10 g
Dextrose 7g

For potato agars made from real potatoes label PP so PPPDA or PPPDY for example
For long term storage/ wood decomposers Add 10 g sawdust and label with :SD
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Cornmeal Malt Agar CMMA

H2O 500 mL
Agar 10 g
Cornmeal 10 g
Dextrose 7g

Spore Germination Agar #1 SGA1 10 g cornmeal and 10 g agar can

H2O 500 mL
be substituted for the Difco
Agar 10 g
Cornmeal agar.
Difco Cornmeal Agar 8.5 g
Glucose (Dextrose) 1g
Yeast 0.5 g

10 g cornmeal and 10 g agar can

Spore Germination Agar #2 SGA2
be substituted for the Difco
H2O 500 mL
Difco Cornmeal Agar 8.5 g Cornmeal agar.
Glucose (Dextrose) 3.5 g
Sucrose 5g
Yeast 0.5 g
KH2PO4 0.5 g

Spore Germination Agar #3 SGA3

H2O 500 mL
Agar 10 g
Cornmeal 10 g
Malt 0.8 g

10 g cornmeal and 10 g agar can

Spore Germination Agar #4 SGA4
be substituted for the Difco
H2O 500 mL
Difco Cornmeal Agar 8.5 g Cornmeal agar.
Glucose 1g
Sucrose 1.5 g
Yeast 0.5 g

CHEMICAL MEDIA

Malt with Activated Carbon R7

and KOH
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H2O 500 mL
Agar 10 g
Epsom Salts 0.25 g
Activated Carbon 1.3 g
Malt 12.5 g
Humus 2.5 g
KOH 1% 20 mL

Organic Medium Agar OMA

H2O 500 mL
Agar 10 g
Glucose 5g
Peptone 0.5 g
Yeast 0.05 g
KH2PO4 1g
MgSO4x7H2O or 0.15 g or
MgSO4 0.075 g

Czapek's Medium Czapek's

H2O 500 mL
Agar 10 g
NaNO3 1.5 g
K2HPO4 0.5 g
MgSO4 0.25 g
KCl 0.25 g
FeSO4 or 0.0187 g or
Fe SO4x7H2O 0.0094 g
Dextrose 15 g

JUICE/BROTH MEDIA

V8 juice Agar V8A

H2O 500 mL
Agar 10 g
CaCO3 1.5 g
V8 Juice 100 mL

Beef Broth Agar BBA

H2O 500 mL
Agar 10 g
Beef Broth 20 mL
Malt 5g
Yeast 1g
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Chicken Broth Agar CBA

H2O 500 mL
Agar 10 g
Chicken Broth 20 mL
Malt 5g
Yeast 1g

Butternut Squash Soup Agar BNSA

H2O 500 mL
Agar 10 g
Butternut squash soup 20 mL
Malt 5g
Yeast 1g

Beef Yeast Agar BYA

H2O 300 mL
Agar 10 g
Yeast 2g
Beef Broth 200 mL

MISCELLANEOUS MEDIA

Brine Agar BA
H2O 500 mL
Agar 10 g
Brine 10 g

Sea Water Agar SWA Dissolve beef extract and peptone by

H2O 125 mL heating H2O. Adjust pH to 7.8, and
Agar 10 g
boil for 10 minutes. Readjust pH to
Sea Water 375 mL
7.3 and add agar.
Beef Extract 5g
Peptone 5g
To Make Artificial Sea
Water
NaCl 14.065 g
KCl 0.385 g
CaCl2 0.60 g
MgCl2 1.275 g
NaHCO3 0.065 g
MgSO4x7H2O 1.75 g
H2O 500 mL
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Nutrient Agar NA
H2O 500 mL
Pre-mixed NA 12 g

Honey Agar HON

H2O 500 mL
Agar 10 g
Honey 16 g

Nutrient Agar with Honey NAH

H2O 500 mL
Pre-mixed NA 12 g
Honey 16 g

Lysogeny Broth LB Also use as broth (no agar).

Agar 10 g
H2O 500 mL
Yeast 2.5 g
Peptone 5g
Dextrose 10 g
Or replace yeast, peptone and 12.5 g
dextrose with LB pre-mix

Yeast Peptone Dextrose YPD

Agar 10 g
H2O 500 mL
Yeast 5g
Peptone 10 g
Dextrose 10 g

Yeast Peptone Agar YEP

Agar 10 g
H2O 500 mL
Yeast 10 g
Peptone 20 g
Tryptophan 0.32 g

V8 and Honey V8HEM

H2O 500 mL
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Agar 10 g
Honey 16 g
V8 Juice 80 mL
Egg Shell 0.4 g
Milo 1g

Beet Root Agar BRA

H2O 500 mL
Agar 10 g
Beet Root Supplement 3g
Malt 10 g

Peaches Malt Agar PMA

H2O 500 mL
Agar 10 g
Peaches (canned) 10 g
Dextrose 7g

Spirulina Agar SPA

H2O 500 mL
Agar 10 g
Spirulina supplement 3g
Malt 10 g

Nori Agar NorA

H2O 500 mL
Agar 10 g
Nori 3g
Malt 10 g

Bean Soup Agar BSA

H2O 400 mL
Agar 10 g
Bean Soup 100 mL

1) Dissolve agar into water

Mannitol Egg Yolk MYP
Polymyxin Agar 2) Sterilize
H2O 500 mL 3) cool to 45-50◦C
Agar 10 g 4) Add egg yolk solution and
Pre-mixed MYP 23 g antibiotic
Clycloheximide :C 2g
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Egg Yolk Solution` 25 mL

Antibiotic vial P 4.1 mL

Water Agar H2O

H2O 500 mL
Agar 10 g

Fish Disease Renibacterium FDR Adjust pH to 6.8 before autoclaving.

Salmoninarum Charcoal
Agar
H2O 500 mL
Agar 10 g
Peptone 5g
Yeast 0.3 g
L-cysteine hydrochloride 0.5 g
Activated Carbon 0.5 g

Variant #1 V1
H2O 500 mL
Agar 10 g
Dry sprouted millet 4g
Yeast 1g
Malt 10 g

Variant #2 V2
H2O 500 mL
Agar 10 g
Dry sprouted oats 4g
Yeast 1g
Malt 10 g

Variant #3 V3
H2O 500 mL
Agar 10 g
Live sprouted millet 8g
Yeast 1g
Malt 10 g
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Variant #4 V4
H2O 500 mL
Agar 10 g
Live sprouted oats 8g
Yeast 1g
Malt 10 g

Variant #5 V5
H2O 500 mL
Agar 10 g
Fresh Ground Corn 12 g
Yeast 1g
Malt 10 g

For all dry Sprouted grains: place on damp cloth (Scott towel) for about 2 days. Dry for 1 day
(completely dry). Grind.
For Live sprouted grains: place on damp cloth for about two days. Grind.

For any fresh insect media: Freeze before grinding

Variant #6 V6
H2O 500 mL
Agar 10 g
Fresh ground crickets 8g
Yeast 1g
Malt 10 g

Variant #7 V7
H2O 500 mL
Agar 10 g
Fresh ground crickets 12 g
Yeast 1g
Malt 10 g

Crickets and Corn CRC

H2O 500 mL
Agar 10 g
Fresh ground crickets 8g
Fresh Ground Corn 8g
Yeast 1g
Malt 10 g
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Variant #9 V9
H2O 500 mL
Agar 10 g
Fresh ground crickets 8g
Fresh Ground Corn 8g
Oyster Shell 0.8 g
Yeast 1g
Malt 10 g

Variant #10 V10

H2O 500 mL
Agar 10 g
Fresh ground crickets 8g
Fresh Ground Corn 8g
Oats 8g
Yeast 1g
Malt 10 g

For any fresh insect media: Freeze before grinding

Variant #11 V11

H2O 500 mL
Agar 10 g
Fresh Ground Corn 8g
Oyster shell 0.8 g
Yeast 1g
Malt 10 g

Variant #12 (MYA with 2x (MYx2A)

Yeast)
H2O 500 mL
Agar 10 g
Yeast 2g
Malt 10 g

Photo-bacterium medium PBM Adjust pH to 7.2-7.5 before

H2O Bring solution up to 500 mL
sterilizing.
Agar 10 g
Tris 4g
NH4 0.4 g
Glycerol 0.5 g
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Tryptone 5g
Yeast 2.5 g
Sea Water aquarium salt 16.5 g
CaCo3 0.5 g

The antibiotic of choice needs to be added to

Antibiotic Agar AB agar after sterilization. in 500mg/500 mL.
H2O 500 mL 1) Grind antibiotic into powder under the
Agar 10 g hood.
Levaquin :L 25 g 2)Add about 10-15 mL pre-sterilized coded
Doxycycline :D 25 g H2O.
Amoxicillin :A 25 g 3) Mix well.
Sefdinir :CEF 25 g 4) When agar has cooled to about 50-60◦C
add antibiotic.
Ciprofloxacin :CIP 25 g
Azithromyacin :AZI 25 g
Cephalexin :CLX 25 g

Cycloheximide (Dr. Dan’s Favorite antibiotic)

Put 1 mL of 1% cycloheximide solution in through filtered syringe and into any 1L of media (malt and
potato based recommended). To make 1% cycloheximide solution weight out 0.1 g of cycloheximide
and bring solution up to 10 mL with water.

Vermiculite Cakes Verm Cakes Makes 5 cakes.

Sorghum flour (ground milo) 1 cup
H2O 1 cup
Vermiculite 2 cup

Soak plugs in water for at least 24 hours.

Plug Recipe Plug Recipe Drain. Mix with gypsum and Wheat bran
50, 000 plugs 1 cup till each plug is coated. Sterilize in
H2O Enough to soak plugs autoclave for 1-2 hours. Inoculate and
Garbage bag 1 seal. When mycelium covers plugs drill
Gypsum 4- 8 cups
hole in log place in plug and seal with
Wheat Bran 6-8 quarts
wax. Logs will fruit in 6 months to two
years.

Media for Liquid Cultures LC

H2O 500 mL
Malt 10 g
Peptone 1g
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Yeast 0.3 g
Vegetable oil 5 drops
Ground Grain (sorghum, oats, 1g
etc)

EXPERIMENTAL AGAR

Boil 1 bag of baby carrots in 500 mL

Carrot Agar CAR
water. Mash carrots. Freeze leftovers.
Agar 10 g
H2O 500 mL
Carrot mash 20 g

Carrot Malt Agar CMA Boil 1 bag of baby carrots in 500 mL

Agar 10 g water. Mash carrots. Freeze leftovers.
H2O 500 mL
Carrot mash 20 g
Malt 5g

Complete Media CM
Agar 10 g
H2O 500 mL
Sucrose 15 g
Ammonium tartrate (NH4-) 2.5 g
Ammonium Nitrate (NH4NO3) 0.5 g
Magnesium sulfate 0.25 g
heptahydrate (MgSO4x7H2O)
Sodium Chloride (NaCl) 0.5 g
Calcium chloride (CaCl2) 0.065 g
Yeast 0.5 g
Monopotassium phosphate 0.5 g
(KH2PO4)

Sunflower Seed Agar SFS

H2O 500 mL
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Agar 10 g
Sunflower seeds 10 g

Almond Agar AA
H2O 500 mL
Agar 10 g
Almonds 10 g

MEDIA NOT TO USE!

Reason: failure for mycelium to thrive

Cricket Food Agar CA
Agar 10 g
H2O 500 mL
Cricket food 20 g

Ovaltine OVAL Reason: failure for mycelium to

H2O 500 mL thrive
Agar 10 g
Ovaltine 10 g

Coffee Raw with Malt C(R)MA Reason: failure for mycelium to

Extract thrive
H2O 500 mL
Agar 10 g
Malt 10 g
Raw Coffee 10 g

Dark Coffee with Malt C(D)MA Reason: failure for mycelium to

Extract thrive
H2O 500 mL
Agar 10 g
Dark Coffee Bean 10 g
Malt 10 g

Nutrient Agar with Soy NAS Reason: Allergies

H2O 500 mL
Agar 10 g
Pre-mixed NA 12 g
Soy beans 2g
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Amaranth Soy Agar ASA Reason: Allergies

H2O 500 mL
Agar 10 g
Amaranth flour 20g
Soy flour 20 g

EntheoGenesis #442 EG442 Reason: Allergies

H2O 500 mL
Agar 10 g
Malted Barley 2g
Soy flour 10 g
Potato Flour 10 g
Brown Rice Flour 10 g
Amaranth Flour 10 g

Malt Extract with Onion MEO Reason: Antifungal

H2O 500 mL
Agar 10 g
Blended onion 10 g
Malt 10 g

Malt Extract with Garlic MEG Reason: Antifungal

H2O 500 mL
Agar 10 g
Blended Galric 10 g
Malt 10 g

Reason: Allergies
Tryptic Soy Agar TSA
H2O 500 mL
Pre-mixed TSA 20 g

Reason: Allergies
Nutrient Agar with Soy NAS
H2O 500 mL
Pre-mixed NA 12 g
Soybeans 5g

Reason: Allergies
Peanut Butter Agar PB
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H2O 500 mL
Agar 10 g
Peanut Butter 5g

Coconut Agar CN Reason: Antifungal

H2O 500 mL
Agar 1g
Coconut milk/juice 10 g

INGREDIENTS TO NEVER USE WITH PRODOCUTION STRAINS

Ingredient Reason
Peanut Butter Allergies
Soy Allergies
Coconut Antifungal
Dairy/Prebiotics Allergies
Eggs Allergies
Tree nut (walnuts, pecans, almonds) Allergies
Garlic Antifungal
Cider Vinegar Failure to thrive
Ovaltine Antifungal
Pomegranate Antifungal
Cinnamon Antifungal
Cloves Antifungal
Curry Antifungal
Cilantro Antifungal
Coffee Failure to thrive