Amino Acids

_= .

Essential
Non -
amino

÷:*
un&_

1.* ④
.
in
body
Histidine
Arginine
,

lysine ,
,

Valine isoleucine ,
methionine ,

leucine , Phenylalanine ,
Tryptophan ,

Threonine
 Isoelectric Point
.

gueutral
I
:

pin, - on -
Coon
, PI =

-
⇐
-
nt
-

gin Y w
-
-

w
you
-

←
- Y
-

%i
-

in own
PKa ,
- -

n
'
pKa , µ
n

Zwiltnion
Connon cotton )
anion
mom

solution in which
concentration
a
the
ion of
The hydrogen
-

acid doesit migrate under the

particular amino
LPI )
influence of an electric field - isoelectric point .
 Amino Acids
② Acidic
:

nzÑ° Yn
Cloy
-

cuz
-
Leroy

¥2 %%
-

pika ,
µ①
Woh at an
azn
-
on into
*
-
in
-
-
-
in -

the
1 I

car unto
cuz
-

-
urn cuz - own

diunion
Zvi Henion Morvant on

Momo cation

|o¥¥-•÷÷
 Amino Acids :
③ Basic
-
not - on - von
3

tap-in y
,

gyyggyp.a-K-ny-m-cw-PKo.rs
④
-

Plan = *
-
Lh Coo
opt-in1
-

- car
on arm I I
,N
-

⑦
-

n
•

1 M¥ Ms Chol , ME -

%,
loan -

monoamine
.

cation
Zwilterion
mono
dilation

1 pI= pkmtpz.FI y
I
9 PM > Pkaz
2PM
PKLPK .
plea , < pity e pKa , Pkazcprt
0 calculate the pi value for following amino acids :

i'
n ,% - on - con
pka=I p±=
✗
1¥
PKa¥ .

tub

Fung Pka=Y Ytz

Y
-
-
won
,
p±=
z

P1 ( cha),
- can pKa=Z_

Xiii ping -

en - urn pka=Y ✗ + 2-
PI=
z
I d
( cha) ,
-
Ñhzpka -_Z
pKa

Ii " Ping PKa=t

coom
¥
-
ch - ✗

PI
thy
=

P
!¥= -0M
pva=Z
a- Draw structures
of alanine at it PH -2
.

Ii , pn=6 1pm =/ °

- -

NK
-

ca - Coon

Luz
11 pm = 2

Fung -

M -

any
↳
Iii pn=6

uzÑ
-

ao
yn
-
-

th
}

in ph -10
Nuz
Y ao
- -
-

↳3
 PH
PHLPI
pH = PI PIL

I 1
1
Anionic form
cationic form zwiltuion -

-
 polypeptide
acids
→ Determination of sequence of amino in

chains
(F) ' "
"

Sanger Sangu reagent

,
① method :

→
,
NO 2

2. 9- Dinino throne benzene

DNFB]
[ 2.4 -

%f¥cÉ É É E- .
.

yn
.

an
.
-

nu
qn
- - -

en
- - -
-

Moz
K ri
R
"

LSMAI.at#n.-,M-ae-ii:-: nn-cn-ii-nu-y-ii---
.

k k' R
"

/ nydntysis
 É

op-TQ.IN
m
.

n
ng En
-
-
-
→ - -

M t
-

" -

'
'
r
r

--
determine N-terminal of
is used to
→
Sanger method
amino acid
.

f
l

4 Hydrolysis of pdytuhtides :

-8 Dipeptide
polypoid chain

Ma
% chair s Ala Val Len Arg Val -
ten , Arg -

pdypehh.de
- -
,
.

Ala
Ars
Arg
-

Val Ley leu -

-

Ma -
Val -

_( Ala - Ual -
Lw -

Arg -1
m
 É 94
E polypeptide chair rat Arg Asp Vol Mis Asp Guy
- - -
-
, , ,
,

His
Arg
-
.

Guy Arp Aep Gly

Val his
v4 his
Argrtrg
-
- -
- - .

+ 44 his Arg -1N

val
Asp
- - -

I Lys ¥5 possible dipeptide

Ala Vai
Gy pyp
- - -

94 Lys
Val
fly
-

Val
-
,
Ala -

,
,

Write the most stable zwiltu ion of glutamic acid .

a-

ping yn
-

w
uh
- own -
-

no ,
-

tha -
cha -

Wy on , -

uz - own
Ño .
Most stable twitter ion
of lysine :

Naming of polypeptide
=

→ Start from N-terminal

amino acids like ) -
lyl )
'

suffix for all

Use Igt
→
as

lent amino aid

Unto suff_
→
Use normal name for

I E- on
É y
-
Nn
-
-

¥ ① ou
-

run -
en -
- nu -

,
'
1 cmon
↳ lhz -
Ph
Alanylphenylulwryfsorine Serine
Phenylalanine
- Ace -
④
Nhz
É -

Ml won
en
yr
- -
- -

'

n on -
cuz Glycyl Valine

th }
③ valyl Glycine
É
:

- -
nu -
uz -
dinoy
Nne
Yn
-

ipr