Department of Medical Laboratory Science

Davao Doctor’s College

HISTOLOLOGY

Name/s: LOQUIRE, MARY CRISCEBEL KYLA S. Date Performed: JANUARY 20, 2022

Course/Yr. /Sec: BMLS 10-2C Date Submitted: JANUARY 20, 2022

ACTIVITY NO. 1

BASIC MICROSCOPY AND ITS USE IN HISTOLOGY

The term microscope comes from the Ancient Greek word “mikros” and “skopein” which means
small and to look respectively. It is an instrument used to see objects that are too small for the naked
eye. A compound light microscope is used to view minute details of cells and primarily tissues. The term
compound refers to the set of lenses (ocular and objective) used simultaneously to magnify the image.
On the other hand, the term light refers to the necessity to use a light source for better viewing of the
object.

As laboratory scientists, microscope is an indispensable tool more specifically in this laboratory

class. Thus, it is the intent of this activity to emphasize its value and show you how it is efficiently and
effectively operated so it may truly serve its purpose.

OBJECTIVES:

1. Explain the significance of each part of the microscope.

2. Explain how the observation procedure is done correctly.

3. Demonstrate efficiently and effectively the proper way of manipulating the microscope.
MATERIALS:

Microscope

Prepared slides

Colored Pencils

GUIDE QUESTIONS:

1. Illustrate the microscope and NO label its parts. (20 POINTS)

Source: https://microbenotes.com/parts-of-a-microscope/

Student Contributor/s: LOQUIRE, MARY CRISCEBEL KYLA S.

2. List down the parts of the microscope and its function. (15 POINTS)

PARTS FUNCTIONS
1. OCULAR LENS or EYEPIECE  A binocular scope has two ocular lenses.
One for each of the eyes, these lenses
have a 10X magnification power. The
overall magnification of the microscope is
given by multiplying the power of the
ocular lens by the power of the objective
lens.
2. ARM  A support for the scope's top part. It also
acts as a handy handle for transporting
3. MECHANICAL STAGE CONTROLS  A geared mechanism used to accurately
move the slide (which is held in place by
the slide clamp).
4. COARSE ADJUSTMENT KNOB  A fast control that enables speedy
focusing by moving the objective lens or
stage up and down. It is used for
preliminary focusing.
5. FINE ADJUSTMENT KNOB  A slow but precise control used to fine
focus the image when viewing at the
higher magnifications.
6. BASE  The component of your microscope that
is supported by a level and sturdy
surface.
7. OCULAR ADJUSTMENT  An adjustment for differences in the
focusing abilities of your eyes.
8. DIOPTIC ADJUSTMENT  A horizontal repositioning of the oculars.
Adjust your vision so that you only see
one field of view with both eyes open.
9. NOSEPIECE  A circular plate with four objective lenses
that may be turned into different
magnification positions.
10. OBJECTIVE LENS  Four separate lenses that magnify the
image (4X, 10X, 40X and 100X) depending
on the objective in use. The lens is
positioned just above the object being
viewed.
OBJECTIVE POWER OBJECTIVE NAME
 4X SCANNING
 10X LOW POWER
 40X HIGH POWER
 100X OIL IMMERSION
 11. SLIDE CLAMP  A clamp to hold the slide on the stage
12. STAGE  A platform for holding the microscope
slide.
13. CONDENSER  A lens that focuses or directs light onto
the slide.
14. IRIS DIAPHRAGM CONTROL  A lever (or revolving disk) that controls
the amount of light that shines on the
slide. Use just enough light to highlight
and contrast the thing on the slide.
15. FILTER HOLDER  This holder houses a blue filter beneath
the substage condenser.
16. . CONDENSER HEIGHT CONTROL  A knob that adjusts the condenser's
height.
17. . LAMP  The light source
18. LAMP SWITCH  Turns the lamp “on” and “off”.

Source:

https://microbenotes.com/parts-of-a-microscope/

Student Contributor/s: LOQUIRE, MARY CRISCEBEL KYLA.

3. How do you care for the microscope? (10 POINTS)

CARRYING

Carry your microscope using two hands, one on the arm or back slot and the other on the base.

TABLE SETTING

Place the microscope on a level, stable support in a location where it will not be disturbed. Be quickly
knocked off to avoid tripping over the rope, coil it.

CLEANING

For resolution, lenses must be clean. Only lens paper or gauze and cleaning solution should be used.
Never wipe your lenses with your finger, handkerchief, paper towels, or spit. Remove no components
for cleaning; doing so causes dust to enter the microscope.

PUTTING AWAY:

Turn off the light and center the mechanical stage. Place the nosepiece in the lowest scanning (4X)
objective position. Remove the slide off the stage and replace it in its rightful location. Wipe any grease
from the stage and lenses with gauze and lens cleaning. Wrap the cord around your forearm. Carefully
transport the microscope with two hands and install it in the appropriate cabinet.
Source: https://www.technologynetworks.com/tn/how-to-guides/how-to-care-for-your-microscope-
313068

Student Contributor/s: LOQUIRE, MARY CRISCEBEL KYLA