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‫ﻡﺇﻑ ﺳﻲ ﺃﺟﺎﺭ • ﺣﻤﺾ ﺭﻭﺳﻮﻟﻴﻚ‬

‫ﺑﻘﺎﻋﺪﺓﻣﺮﻕ ﺇﻑ ﺳﻲ‬
‫ﺍﻟﺼﻴﻎ‬ ‫ﺍﻻﺳﺘﺨﺪﺍﻡﺍﻟﻤﻘﺼﻮﺩ‬
‫ﺩﻳﻔﻜﻮ™ﻡ ‪FC Agar‬‬ ‫ﻳﺘﻢﺍﺳﺘﺨﺪﺍﻡ ‪ m FC Agar‬ﻭ ‪ m FC Broth Base‬ﻣﻊ ﺣﻤﺾ‬
‫ﺍﻟﺼﻴﻐﺔﺍﻟﺘﻘﺮﻳﺒﻴﺔ * ﻟﻜﻞ ﻟﺘﺮ‬ ‫ﺭﻭﺯﻭﻟﻴﻚﻓﻲ ﺯﺭﺍﻋﺔ ﻭﺗﻌﺪﺍﺩ ﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕ ﺍﻟﺒﺮﺍﺯﻳﺔ ﺑﺎﺳﺘﺨﺪﺍﻡ ﺗﻘﻨﻴﺔ‬
‫ﺯ‬ ‫ﺍﻟﺘﺮﺑﺘﻮﺯ ‪ 10.0................... .................................................‬ﺑﺒﺘﻮﻥ‬
‫ﺍﻟﺘﺮﺷﻴﺢﺍﻟﻐﺸﺎﺉﻲ ﻓﻲ ﺩﺭﺟﺎﺕ ﺣﺮﺍﺭﺓ ﻣﺮﺗﻔﻌﺔ‪.‬‬
‫ﺯ‬ ‫ﺍﻟﺒﺮﻭﺗﻴﻦﺭﻗﻢ ‪ 5.0 ...................... ......................... 3‬ﻣﺴﺘﺨﻠﺺ‬
‫ﺯ‬ ‫ﺍﻟﺨﻤﻴﺮﺓ ‪3.0...................................... ...‬‬
‫ﺯ‬ ‫ﺍﻟﻼﻛﺘﻮﺯ ‪ 12.5... .................................................. ..........‬ﺃﻣﻼﺡ‬ ‫ﺍﻟﻤﻠﺨﺺﻭﺍﻟﺸﺮﺡ‬
‫ﺯ‬ ‫ﺍﻟﺼﻔﺮﺍءﺭﻗﻢ ‪ 1.5 ......................... ................................... 3‬ﻛﻠﻮﺭﻳﺪ‬ ‫ﺟﻴﻠﺪﺭﻳﺘﺶﻭﺁﺧﺮﻭﻥ‪1.‬ﺻﺎﻍ ﻭﺳﻴﻄﺎً ﻟﺘﻌﺪﺍﺩ ﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕ ﺍﻟﺒﺮﺍﺯﻳﺔ )‪(MFC‬‬
‫ﺯ‬ ‫ﺍﻟﺼﻮﺩﻳﻮﻡ ‪5.0................................... ......................‬‬
‫ﺯ‬ ‫ﺁﺟﺎﺭ ‪15.0.......... .................................................. .............‬‬ ‫ﺑﺎﺳﺘﺨﺪﺍﻡﺗﻘﻨﻴﺔ ﻣﺮﺷﺢ ﺍﻟﻐﺸﺎء )‪ (MF‬ﺩﻭﻥ ﺗﺨﺼﻴﺐ ﻣﺴﺒﻖ‪ .‬ﻳﺘﻢ‬
‫ﺯ‬ ‫ﺍﻷﻧﻴﻠﻴﻦﺍﻷﺯﺭﻕ ‪0.1 ............................ .....................................‬‬ ‫ﺗﻤﻴﻴﺰﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕ ﺍﻟﺒﺮﺍﺯﻳﺔ )ﺃﻱ ﺗﻠﻚ ﺍﻟﻤﻮﺟﻮﺩﺓ ﻓﻲ ﺑﺮﺍﺯ ﺍﻟﺤﻴﻮﺍﻧﺎﺕ‬
‫ﺩﻳﻔﻜﻮ™‪m FC Broth Base‬‬ ‫ﺫﻭﺍﺕﺍﻟﺪﻡ ﺍﻟﺤﺎﺭ( ﻋﻦ ﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕ ﻣﻦ ﺍﻟﻤﺼﺎﺩﺭ ﺍﻟﺒﻴﺉﻴﺔ ﻣﻦ ﺧﻼﻝ‬
‫ﻳﺘﻜﻮﻥﻣﻦ ﻧﻔﺲ ﺍﻟﻤﻜﻮﻧﺎﺕ ﺑﺪﻭﻥ ﺍﻻﺟﺎﺭ‪.‬‬ ‫ﻗﺪﺭﺗﻬﺎﻋﻠﻰ ﺍﻟﻨﻤﻮ ﻋﻨﺪ ‪ 0.5 ± 44.5‬ﺩﺭﺟﺔ ﻣﺉﻮﻳﺔ‪2.‬‬

‫ﺩﻳﻔﻜﻮ™ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ‬ ‫ﺗﺤﺪﺩﺍﻟﻌﺪﻳﺪ ﻣﻦ ﺇﺟﺮﺍءﺍﺕ ﺍﻟﺘﺮﺷﻴﺢ ﺍﻟﻐﺸﺎﺉﻲ "ﺍﻟﻄﺮﻕ ﺍﻟﻘﻴﺎﺳﻴﺔ"‬

‫ﺣﻤﺾﺍﻟﻮﺭﺩ ‪ 1 ................ ................................................‬ﺟﻢ ‪ /‬ﻗﺎﺭﻭﺭﺓ‬ ‫ﻭﺳﻴﻂ‪ m FC‬ﻻﺧﺘﺒﺎﺭ ﺍﻟﻤﻴﺎﻩ‪2-4.‬ﺗﺤﺪﺩ ﺟﻤﻌﻴﺔ ﺍﻟﺼﺤﺔ ﺍﻟﻌﺎﻣﺔ ﺍﻷﻣﺮﻳﻜﻴﺔ‬
‫* ﻣﻌﺪﻟﺔ ﻭ ‪ /‬ﺃﻭ ﻣﻜﻤﻠﺔ ﺣﺴﺐ ﺍﻻﻗﺘﻀﺎء ﻟﺘﻠﺒﻴﺔ ﻣﻌﺎﻳﻴﺮ ﺍﻷﺩﺍء‪.‬‬
‫)‪ (APHA‬ﻭﺳﻂ ‪ FC‬ﻭﺣﻀﺎﻧﺔ ﻋﻨﺪ ‪ 0.5 ± 44.5‬ﺩﺭﺟﺔ ﻣﺉﻮﻳﺔ ﻓﻲ‬
‫ﺍﺗﺠﺎﻫﺎﺕﺍﻟﺘﺤﻀﻴﺮ ﻣﻦ ﺍﻟﻤﻨﺘﺞ‬ ‫ﺇﺟﺮﺍءﻣﺮﺷﺢ ﻏﺸﺎء ﺍﻟﻘﻮﻟﻮﻥ ﺍﻟﺒﺮﺍﺯﻱ ‪ ،‬ﻭﺇﺟﺮﺍء ﺍﻟﺤﻀﺎﻧﺔ ﺍﻟﻘﻮﻟﻮﻧﻴﺔ‬
‫ﺍﻟﺒﺮﺍﺯﻳﺔﺍﻟﻤﺘﺄﺧﺮﺓ ﻭﻃﺮﻳﻘﺔ ﺃﺟﺎﺭ ﺛﻨﺎﺉﻴﺔ ﺍﻟﻄﺒﻘﺔ ﻻﺳﺘﻌﺎﺩﺓ ﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕ‬
‫ﺍﻟﻤﺠﻔﻒ‬
‫ﺍﻟﺒﺮﺍﺯﻳﺔﺍﻟﻤﺼﺎﺑﺔ‪2.‬ﺗﺤﺪﺩ ‪AOAC International m FC Agar‬‬
‫ﺩﻳﻔﻜﻮ™ﻡ ‪FC Agar‬‬
‫ﻟﻠﻜﺸﻒﻋﻦ ﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕ ﺍﻟﻜﻠﻴﺔ ﻭﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕ ﺍﻟﺒﺮﺍﺯﻳﺔ ﻓﻲ ﺍﻷﻃﻌﻤﺔ‪3.‬‬
‫‪.1‬ﻋﻠﻖ ‪ 52‬ﺟﻢ ﻣﻦ ﺍﻟﻤﺴﺤﻮﻕ ﻓﻲ ‪ 1‬ﻟﺘﺮ ﻣﻦ ﺍﻟﻤﺎء ﺍﻟﻨﻘﻲ‪ .‬ﺗﺨﻠﻂ ﺟﻴﺪﺍ‪.‬‬

‫‪.2‬ﺳﺨﻦ ﻣﻊ ﺍﻟﺘﻘﻠﻴﺐ ﺍﻟﻤﺘﻜﺮﺭ ﻭﺍﺗﺮﻛﻪ ﻳﻐﻠﻲ ﻟﻤﺪﺓ ﺩﻗﻴﻘﺔ ﻭﺍﺣﺪﺓ‬ ‫ﺗﺤﺪﺩﻭﻛﺎﻟﺔ ﺣﻤﺎﻳﺔ ﺍﻟﺒﻴﺉﺔ ﺍﻷﻣﺮﻳﻜﻴﺔ ﺍﺳﺘﺨﺪﺍﻡ ﻭﺳﻂ ‪ m FC‬ﻓﻲ ﻃﺮﻕ‬
‫ﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕﺍﻟﺒﺮﺍﺯﻳﺔ ﻻﺧﺘﺒﺎﺭ ﺍﻟﻤﻴﺎﻩ ﺑﻄﺮﻳﻘﺔ ‪ MF‬ﺍﻟﻤﺒﺎﺷﺮﺓ ﺃﻭ ﻃﺮﻳﻘﺔ‬
‫ﻟﻴﺬﻭﺏﺍﻟﻤﺴﺤﻮﻕ ﺗﻤﺎﻣﺎً‪.‬‬
‫‪ MF‬ﺍﻟﻤﺘﺄﺧﺮﺓ‪4،5.‬‬
‫‪.3‬ﺃﺿﻒ ‪ 10‬ﻣﻞ ﻣﻦ ﻣﺤﻠﻮﻝ ‪ ٪1‬ﻣﻦ ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ ﻓﻲ ‪ 0.2N‬ﻫﻴﺪﺭﻭﻛﺴﻴﺪ‬
‫ﺍﻟﺼﻮﺩﻳﻮﻡ‪.‬ﺍﺳﺘﻤﺮ ﻓﻲ ﺍﻟﺘﺴﺨﻴﻦ ﻟﻤﺪﺓ ‪ 1‬ﺩﻗﻴﻘﺔ‪ .‬ﻻ ﺗﻐﻠﻖ ﺗﻠﻘﺎﺉﻲ‪.‬‬
‫ﻣﺒﺎﺩﺉﺍﻹﺟﺮﺍء‬
‫‪.4‬ﺇﺫﺍ ﻟﺰﻡ ﺍﻷﻣﺮ ‪ ،‬ﺍﺿﺒﻂ ﺍﻟﺮﻗﻢ ﺍﻟﻬﻴﺪﺭﻭﺟﻴﻨﻲ ‪ 7.4‬ﻣﻊ ‪.1N HCl‬‬
‫‪.‬ﻋﻠﻰﺃﺟﺎﺭ ﻛﻌﺎﻣﻞ ﺗﺮﺳﻴﺦ‪ .‬ﻳﺠﻤﻊ ﻧﻈﺎﻡ ﺍﻟﻤﺆﺷﺮﺍﺕ ﺍﻟﺘﻔﺎﺿﻠﻴﺔ ﺑﻴﻦ‬
‫‪.5‬ﺍﺧﺘﺒﺎﺭ ﻋﻴﻨﺎﺕ ﻣﻦ ﺍﻟﻤﻨﺘﺞ ﺍﻟﻨﻬﺎﺉﻲ ﻟﻸﺩﺍء ﺑﺎﺳﺘﺨﺪﺍﻡ ﺛﻘﺎﻓﺎﺕ ﺗﺤﻜﻢ‬
‫ﺍﻷﺯﺭﻕﺍﻷﻧﻴﻠﻴﻦ ﻭﺣﻤﺾ ﺍﻟﺮﻭﺯﻭﻟﻴﻚ ‪ m FC Agar‬ﻳﺤﺘﻮﻳﺎﻥ ﻋﻠﻰ‬
‫ﻧﻤﻮﺫﺟﻴﺔﻣﺴﺘﻘﺮﺓ‪.‬‬
‫ﺍﻟﺒﺒﺘﻮﻥﻛﻤﺼﺎﺩﺭ ﻟﻠﻜﺮﺑﻮﻥ ﻭﺍﻟﻨﻴﺘﺮﻭﺟﻴﻦ ﻭﺍﻟﻔﻴﺘﺎﻣﻴﻨﺎﺕ ﻭﺍﻟﻤﻌﺎﺩﻥ ‪ ،‬ﻛﻤﺎ‬
‫ﺩﻳﻔﻜﻮ™‪m FC Broth Base‬‬ ‫ﺃﻥﻣﺴﺘﺨﻠﺺ ﺍﻟﺨﻤﻴﺮﺓ ﻳﻮﻓﺮ ﻓﻴﺘﺎﻣﻴﻨﺎﺕ ﺏ ﺍﻟﻤﻌﻘﺪﺓ ﺍﻟﺘﻲ ﺗﺤﻔﺰ ﻧﻤﻮ‬
‫‪.1‬ﻋﻠﻖ ‪ 3.7‬ﺟﻢ ﻣﻦ ﺍﻟﻤﺴﺤﻮﻕ ﻓﻲ ‪ 100‬ﻣﻞ ﻣﻦ ﺍﻟﻤﺎء ﺍﻟﻨﻘﻲ‪.‬‬ ‫ﺍﻟﺒﻜﺘﻴﺮﻳﺎ‪.‬ﺍﻟﻼﻛﺘﻮﺯ ﻫﻮ ﻛﺮﺑﻮﻫﻴﺪﺭﺍﺕ‪ .‬ﺗﻤﻨﻊ ﺃﻣﻼﺡ ﺍﻟﺼﻔﺮﺍء ﺭﻗﻢ ‪ 3‬ﻧﻤﻮ‬
‫‪.2‬ﺃﺿﻒ ‪ 1‬ﻣﻞ ﻣﻦ ﻣﺤﻠﻮﻝ ‪ ٪1‬ﻣﻦ ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ ﻓﻲ ‪ 0.2N‬ﻫﻴﺪﺭﻭﻛﺴﻴﺪ ﺍﻟﺼﻮﺩﻳﻮﻡ‪.‬‬ ‫ﺍﻟﺒﻜﺘﻴﺮﻳﺎﻣﻮﺟﺒﺔ ﺍﻟﺠﺮﺍﻡ‪ .‬ﻳﺤﺘﻮﻱ ‪ m FC Broth Base‬ﻭ ‪m FC Agar‬‬
‫‪.3‬ﺇﺫﺍ ﻟﺰﻡ ﺍﻷﻣﺮ ‪ ،‬ﺍﺿﺒﻂ ﺍﻟﺮﻗﻢ ﺍﻟﻬﻴﺪﺭﻭﺟﻴﻨﻲ ‪ 7.4‬ﻣﻊ ‪.1N HCl‬‬
‫‪.4‬ﺍﻟﺤﺮﺍﺭﺓ ﺣﺘﻰ ﺍﻟﻐﻠﻴﺎﻥ‪ .‬ﻻ ﺗﻐﻠﻖ ﺗﻠﻘﺎﺉﻲ‪.‬‬ ‫ﻣﺴﺘﻌﻤﺮﺍﺕﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕ ﺍﻟﺒﺮﺍﺯﻳﺔ ﺯﺭﻗﺎء‪ .‬ﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕ ﻏﻴﺮ ﺍﻟﺒﺮﺍﺯﻳﺔ ﻭﺍﻟﻜﺎﺉﻨﺎﺕ‬
‫‪.5‬ﺗﺒﺮﺩ ﻗﺒﻞ ﺍﻻﺳﺘﻐﻨﺎء‪.‬‬ ‫ﺍﻟﺤﻴﺔﺍﻷﺧﺮﻯ ﺭﻣﺎﺩﻳﺔ ﺍﻟﻠﻮﻥ ﺇﻟﻰ ﻛﺮﻳﻤﻴﺔ ﺍﻟﻠﻮﻥ‪.‬‬
‫‪.6‬ﺍﺧﺘﺒﺎﺭ ﻋﻴﻨﺎﺕ ﻣﻦ ﺍﻟﻤﻨﺘﺞ ﺍﻟﻨﻬﺎﺉﻲ ﻟﻸﺩﺍء ﺑﺎﺳﺘﺨﺪﺍﻡ ﺛﻘﺎﻓﺎﺕ ﺗﺤﻜﻢ‬
‫ﻧﻤﻮﺫﺟﻴﺔﻣﺴﺘﻘﺮﺓ‪.‬‬
‫ﺩﻳﻔﻜﻮ™ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ‬
‫ﺗﺤﻀﻴﺮﻣﺤﻠﻮﻝ ‪ ، ٪1‬ﺇﺫﺍﺑﺔ ‪ 1‬ﻏﺮﺍﻡ ﻓﻲ ‪ 100‬ﻣﻞ ﻣﻦ ‪ 0.2N‬ﻫﻴﺪﺭﻭﻛﺴﻴﺪ ﺍﻟﺼﻮﺩﻳﻮﻡ‪.‬‬

‫ﺍﻹﺻﺪﺍﺭﺍﻟﺜﺎﻧﻲ ‪Difco ™ & BBL ™ Manual ،‬‬

 ‫ﻣﺮﺍﻗﺒﺔﺟﻮﺩﺓ ﺍﻟﻤﺴﺘﺨﺪﻡ‬
‫ﺍﻻﺳﺘﺠﺎﺑﺔﺍﻟﺜﻘﺎﻓﻴﺔ‬ ‫ﻣﻮﺍﺻﻔﺎﺕﺍﻟﻬﻮﻳﺔ‬
‫ﺩﻳﻔﻜﻮ™‪ m FC Broth Base‬ﺃﻭ ‪m FC Agar‬‬ ‫ﺩﻳﻔﻜﻮ™ﻡ ‪FC Agar‬‬
‫ﺗﺤﻀﻴﺮﺍﻟﻮﺳﻴﻂ ﻟﻜﻞ ﺗﻮﺟﻴﻬﺎﺕ ﺍﻟﺘﺴﻤﻴﺔ ﺑﺎﺳﺘﺨﺪﺍﻡ ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ ‪ .٪1‬ﺑﺎﺳﺘﺨﺪﺍﻡ ﺗﻘﻨﻴﺔ ﺍﻟﺘﺮﺷﻴﺢ‬ ‫ﺑﻴﺞﻣﻊ ﻟﻮﻥ ﺃﺯﺭﻕ ﺧﻔﻴﻒ ﺇﻟﻰ ﺃﺯﺭﻕ ‪ ،‬ﻳﺘﺪﻓﻖ ﺑﺤﺮﻳﺔ ‪،‬‬ ‫ﻣﻈﻬﺮﺟﺎﻑ‪:‬‬
‫ﺍﻟﻐﺸﺎﺉﻲ ‪،‬ﺗﻠﻘﻴﺢ ﺍﻟﺼﻔﺎﺉﺢ ﻭﺍﺣﺘﻀﺎﻧﻬﺎ ﻋﻨﺪ ﺩﺭﺟﺔ ﺣﺮﺍﺭﺓ ‪ 0.5 ± 44.5‬ﺩﺭﺟﺔ ﻣﺉﻮﻳﺔ ﻟﻤﺪﺓ ‪2 ± 24‬‬ ‫ﻣﺘﺠﺎﻧﺲ‪.‬‬
‫ﺳﺎﻋﺔ‪.‬‬ ‫ﻣﺤﻠﻮﻝ‪ ، ٪5.2‬ﻗﺎﺑﻞ ﻟﻠﺬﻭﺑﺎﻥ ﻓﻲ ﺍﻟﻤﺎء ﺍﻟﻨﻘﻲ ﻋﻨﺪ‬ ‫ﺣﻞ‪:‬‬
‫ﻣﺴﺘﻌﻤﺮﺓ‬ ‫ﺗﻠﻘﻴﺢ‬
‫ﺍﻟﻐﻠﻴﺎﻥ‪.‬ﺑﺪﻭﻥ ‪ ٪1‬ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ‪ :‬ﺍﻷﺯﺭﻕ ‪ ،‬ﻗﻠﻴﻼً ﺟﺪﺍً‬
‫ﻟﻮﻥ‬ ‫ﺍﺳﺘﻌﺎﺩﺓ‬ ‫‪CFU‬‬ ‫‪™ATCC‬‬ ‫ﺍﻟﺘﻨﻈﻴﻢ‬ ‫ﺇﻟﻰﺑﺮﺍﻕ ﻗﻠﻴﻼ ً‪ ،‬ﻗﺪ ﻳﻜﻮﻥ ﻟﻪ ﺗﺮﺳﺐ ﻃﻔﻴﻒ‪ .‬ﻣﻊ ‪٪1‬‬
‫ﺣﻤﺾﺭﻭﺯﻭﻟﻴﻚ‪ :‬ﺃﺣﻤﺮ ﺗﻮﺕ ﺑﺮﻱ ‪ ،‬ﺑﺮﺍﻕ ﻗﻠﻴﻼ ً‪ ،‬ﻗﺪ‬
‫‪-‬‬ ‫ﻣﻠﺤﻮﻅ‬ ‫‪3‬‬ ‫‪10×2-310‬‬ ‫‪19433‬‬ ‫ﺍﻟﻤﻜﻮﺭﺍﺕﺍﻟﻤﻌﻮﻳﺔ‬
‫ﻳﻜﻮﻥﻟﻪ ﺗﺮﺳﺒﺎﺕ ﻃﻔﻴﻔﺔ‪.‬‬
‫ﻣﻜﺘﻤﻞ‬ ‫ﺍﻟﺒﺮﺍﺯﻳﺔ‬
‫ﻛﺒﺖ‬ ‫ﺑﺪﻭﻥﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ ‪ - ٪1‬ﺍﻷﺯﺭﻕ ‪ ،‬ﺑﺮﺍﻕ ﻗﻠﻴﻼ ً‪ ،‬ﻗﺪ‬ ‫ﺍﻟﻤﻈﻬﺮﺍﻟﺠﺎﻫﺰ‪:‬‬
‫ﻳﻜﻮﻥﻟﻪ ﺗﺮﺳﺒﺎﺕ ﻃﻔﻴﻔﺔ‪.‬‬
‫ﻩ‬
‫ﻣﻊ‪ ٪1‬ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ ‪ -‬ﺃﺣﻤﺮ ﺍﻟﺘﻮﺕ ﺍﻟﺒﺮﻱ ‪ ،‬ﺑﺮﺍﻕ‬
‫ﻗﻠﻴﻼ ً‪،‬ﻗﺪ ﻳﻜﻮﻥ ﻟﻪ ﺭﻭﺍﺳﺐ ﻃﻔﻴﻔﺔ‪.‬‬
‫ﻩ‬ ‫ﺭﺩﻓﻌﻞ ‪٪5.2‬‬
‫ﺃ‬ ‫ﺍﻟﺮﻗﻢﺍﻟﻬﻴﺪﺭﻭﺟﻴﻨﻲ ‪) 0.2 ± 7.4‬ﺑﺪﻭﻥ ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ ‪(٪1‬‬ ‫ﺍﻟﺤﻞﻋﻨﺪ ‪ 25‬ﺩﺭﺟﺔ ﻣﺉﻮﻳﺔ‪:‬‬

‫ﺩﻳﻔﻜﻮ™‪m FC Broth Base‬‬

‫ﺑﻴﺞﻣﻊ ﻟﻮﻥ ﺃﺯﺭﻕ ﺧﻔﻴﻒ ﺇﻟﻰ ﺃﺯﺭﻕ ‪ ،‬ﻳﺘﺪﻓﻖ ﺑﺤﺮﻳﺔ ‪،‬‬ ‫ﻣﻈﻬﺮﺟﺎﻑ‪:‬‬
‫ﻣﺘﺠﺎﻧﺲ‪.‬‬
‫ﻣﺤﻠﻮﻝ‪ ، ٪3.7‬ﻗﺎﺑﻞ ﻟﻠﺬﻭﺑﺎﻥ ﻓﻲ ﺍﻟﻤﺎء ﺍﻟﻨﻘﻲ ﻋﻨﺪ‬ ‫ﺣﻞ‪:‬‬
‫ﺍﻟﻐﻠﻴﺎﻥ‪.‬ﺍﻟﻤﺤﻠﻮﻝ ﺃﺯﺭﻕ ‪ ،‬ﺑﺮﺍﻕ ﻗﻠﻴﻼ ً‪ ،‬ﻗﺪ ﻳﻜﻮﻥ ﻟﻪ‬
‫ﺭﺍﺳﺐ‪.‬‬
‫ﺑﺪﻭﻥﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ ‪ - ٪1‬ﺍﻷﺯﺭﻕ ‪ ،‬ﺑﺮﺍﻕ ﻗﻠﻴﻼ ً‪ ،‬ﻗﺪ‬ ‫ﺍﻟﻤﻈﻬﺮﺍﻟﺠﺎﻫﺰ‪:‬‬
‫ﻳﻜﻮﻥﻟﻪ ﺗﺮﺳﺒﺎﺕ ﺩﻗﻴﻘﺔ ﺟﺪﺍ‪ً.‬‬
‫ﻣﻊ‪ ٪1‬ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ ‪ -‬ﺃﺣﻤﺮ ﺍﻟﺘﻮﺕ ﺍﻟﺒﺮﻱ ‪ ،‬ﺑﺮﺍﻕ‬
‫ﻗﻠﻴﻼ ً‪،‬ﻗﺪ ﻳﻜﻮﻥ ﻟﻪ ﺗﺮﺳﺐ ﻃﻔﻴﻒ‪.‬‬
‫ﺭﺩﻓﻌﻞ ‪٪3.7‬‬
‫ﺍﻟﺮﻗﻢﺍﻟﻬﻴﺪﺭﻭﺟﻴﻨﻲ ‪) 0.2 ± 7.4‬ﺑﺪﻭﻥ ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ ‪(٪1‬‬ ‫ﺍﻟﺤﻞﻋﻨﺪ ‪ 25‬ﺩﺭﺟﺔ ﻣﺉﻮﻳﺔ‪:‬‬

‫ﺩﻳﻔﻜﻮ™ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ‬
‫ﺑﻨﻲﻣﺤﻤﺮ ﺩﺍﻛﻦ ﻣﻊ ﺟﺰﻳﺉﺎﺕ ﺧﻀﺮﺍء ﻣﻌﺪﻧﻴﺔ ‪،‬‬ ‫ﻣﻈﻬﺮﺟﺎﻑ‪:‬‬
‫ﻣﺴﺤﻮﻕﺑﻠﻮﺭﻱ ﻧﺎﻋﻢ ﻳﺘﺪﻓﻖ ﺑﺤﺮﻳﺔ‪.‬‬
‫ﻣﺤﻠﻮﻝ‪ ، ٪1.0‬ﻗﺎﺑﻞ ﻟﻠﺬﻭﺑﺎﻥ ﻓﻲ ‪ 0.2N‬ﻫﻴﺪﺭﻭﻛﺴﻴﺪ ﺍﻟﺼﻮﺩﻳﻮﻡ‪.‬‬ ‫ﺣﻞ‪:‬‬
‫ﺍﻟﻤﺤﻠﻮﻝﻟﻮﻧﻪ ﺃﺣﻤﺮ ﻏﺎﻣﻖ ‪ ،‬ﻭﺍﺿﺢ ﺇﻟﻰ ﺑﺮﺍﻕ ﺇﻟﻰ ﺣﺪ ﻣﺎ‪.‬‬

‫ﻧﺘﺎﺉﺞﻣﺘﻮﻗﻌﺔ‬
‫ﺳﺘﻜﻮﻥﻣﺴﺘﻌﻤﺮﺍﺕ ﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕ ﺍﻟﺒﺮﺍﺯﻳﺔ ﺑﺪﺭﺟﺎﺕ ﻣﺨﺘﻠﻔﺔ ﻣﻦ ﺍﻟﻠﻮﻥ ﺍﻷﺯﺭﻕ‪.‬‬
‫ﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕﻏﻴﺮ ﺍﻟﺒﺮﺍﺯﻳﺔ ﻟﻮﻧﻬﺎ ﺭﻣﺎﺩﻱ ﺇﻟﻰ ﻛﺮﻳﻤﻲ‪.‬‬
‫ﺣﺪﻭﺩﺍﻹﺟﺮﺍء‬
‫ﻳﻤﻜﻦﻣﻼﺣﻈﺔ ﻋﺪﺩ ﻗﻠﻴﻞ ﻣﻦ ﺍﻟﻤﺴﺘﻌﻤﺮﺍﺕ ﺍﻟﻘﻮﻟﻮﻧﻴﺔ ﻏﻴﺮ ﺍﻟﺒﺮﺍﺯﻳﺔ‬
‫ﻋﻠﻰﻭﺳﺎﺉﻂ ‪ m FC‬ﺑﺴﺒﺐ ﺍﻹﺟﺮﺍء ﺍﻻﻧﺘﻘﺎﺉﻲ ﻟﺪﺭﺟﺔ ﺍﻟﺤﺮﺍﺭﺓ‬
‫ﺍﻟﻤﺮﺗﻔﻌﺔﻭﺇﺿﺎﻓﺔ ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ‪ .‬ﻗﺪ ﻳﻜﻮﻥ ﻣﻦ ﺍﻟﻤﻔﻴﺪ ﺭﻓﻊ ﺩﺭﺟﺔ‬
‫ﺍﻟﺤﺮﺍﺭﺓﺇﻟﻰ ‪ 0.2 ± 45‬ﺩﺭﺟﺔ ﻣﺉﻮﻳﺔ ﻟﻠﺘﺨﻠﺺ ﻣﻨﻬﺎﻛﻠﻴﺒﺴﻴﻼ ﺳﻼﻻﺕ‬
‫ﻣﻦﻣﺠﻤﻮﻋﺔ ﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕ ﺍﻟﺒﺮﺍﺯﻳﺔ‪6.‬‬
‫‪.4‬ﺿﻊ ﺍﻷﻃﺒﺎﻕ ﻓﻲ ﺃﻛﻴﺎﺱ ﺑﻼﺳﺘﻴﻜﻴﺔ ﻭﺍﺣﻀﻨﻬﺎ ‪ ،‬ﻋﻦ ﻃﺮﻳﻖ ﺍﻟﻐﻤﺮ ‪ ،‬ﻓﻲ ﺣﻤﺎﻡ ﻣﺎﺉﻲ‬
‫ﻣﺮﺍﺟﻊ‬
‫‪.1‬ﺟﻴﻠﺪﺭﻳﺘﺶ ‪ ،‬ﻫﻮﻑ ﻭﺑﻴﺴﺖ‪ .1965 .‬ﺝ‪ .‬ﺟﻤﻌﻴﺔ ﺃﻋﻤﺎﻝ ﺍﻟﻤﻴﺎﻩ‪.208 :57.‬‬
‫‪.2‬ﺇﻳﺘﻮﻥ ﻭﺭﺍﻳﺲ ﻭﺑﻴﺮﺩ )ﻣﺤﺮﺭ(‪ .2005 .‬ﺍﻟﻄﺮﻕ ﺍﻟﻘﻴﺎﺳﻴﺔ ﻟﻔﺤﺺ ﺍﻟﻤﻴﺎﻩ ﻭﺍﻟﺼﺮﻑ ﺍﻟﺼﺤﻲ ‪ ،‬ﺍﻟﻄﺒﻌﺔ ‪ ، 21‬ﻋﺒﺮ ﺍﻹﻧﺘﺮﻧﺖ‪.‬‬
‫ﺍﻟﺮﺍﺑﻄﺔﺍﻷﻣﺮﻳﻜﻴﺔ ﻟﻠﺼﺤﺔ ﺍﻟﻌﺎﻣﺔ ‪ ،‬ﻭﺍﺷﻨﻄﻦ ﺍﻟﻌﺎﺻﻤﺔ‬
‫‪.3‬ﻫﻮﺭﻭﻳﺘﺰ )ﻣﺤﺮﺭ(‪ .2007 .‬ﺍﻷﺳﺎﻟﻴﺐ ﺍﻟﺮﺳﻤﻴﺔ ﻟﺘﺤﻠﻴﻞ ‪ AOAC‬ﺍﻟﺪﻭﻟﻴﺔ ‪ ،‬ﺍﻟﻄﺒﻌﺔ ‪ ، 18‬ﻋﻠﻰ ﺍﻹﻧﺘﺮﻧﺖ‪ AOAC .‬ﺍﻟﺪﻭﻟﻴﺔ‪.‬‬
‫ﺟﺎﻳﺜﺮﺳﺒﻴﺮﺝ ‪،‬ﻣﺎﺭﻳﻼﻧﺪ‪.‬‬
‫‪.4‬ﻭﻛﺎﻟﺔ ﺣﻤﺎﻳﺔ ﺍﻟﺒﻴﺉﺔ ﺍﻷﻣﺮﻳﻜﻴﺔ‪ .1992 .‬ﺩﻟﻴﻞ ﺗﺼﺪﻳﻖ ﻣﻌﺎﻣﻞ ﺗﺤﻠﻴﻞ ﻣﻴﺎﻩ ﺍﻟﺸﺮﺏ‪ .EPA-814B-92-002 .‬ﻣﻜﺘﺐ‬
‫ﺍﻟﻤﻴﺎﻩﺍﻟﺠﻮﻓﻴﺔ ﻭﻗﺴﻢ ﺍﻟﺪﻋﻢ ﺍﻟﻔﻨﻲ ‪ ، USEPA ،‬ﺳﻴﻨﺴﻴﻨﺎﺗﻲ ‪ ،‬ﺃﻭﻫﺎﻳﻮ‪.‬‬
‫ﺇﺟﺮﺍء‬
‫ﺩﻳﻔﻜﻮ™ﻡ ‪FC Agar‬‬
‫‪.1‬ﺗﺤﻀﻴﺮ ﻭﺳﻂ ﺃﺟﺎﺭ ﻣﻦ ﺍﻟﻘﺎﻋﺪﺓ ﺍﻟﻤﺠﻔﻔﺔ ﻭﻓﻘﺎً ﻟﺘﻮﺟﻴﻬﺎﺕ‬
‫ﺍﻟﻤﻠﺼﻖﻭﺇﺿﺎﻓﺔ ﻣﺤﻠﻮﻝ ﺣﻤﺾ ﺍﻟﻮﺭﺩ‪.‬‬
‫‪.2‬ﺻﺐ ﺍﻷﺟﺎﺭ ﺍﻟﻤﻨﺼﻬﺮ ‪ ،‬ﺍﻟﻤﺒﺮﺩ ﻣﺴﺒﻘﺎً ﺇﻟﻰ ‪ 50-45‬ﺩﺭﺟﺔ ﻣﺉﻮﻳﺔ ﻓﻲ‬
‫ﺃﻃﺒﺎﻕﺑﻼﺳﺘﻴﻜﻴﺔ ﺧﺎﺻﺔ ﻣﺤﻜﻤﺔ ﺍﻟﻐﻠﻖ ﻭﺍﺗﺮﻛﻬﺎ ﺗﺘﺼﻠﺐ‪.‬‬
‫‪.3‬ﻗﻢ ﺑﻠﻒ ﻣﺮﺷﺢ ﺍﻟﻐﺸﺎء ﺍﻟﻤﺴﺘﺨﺪﻡ ﻟﺠﻤﻊ ﻋﻴﻨﺔ ﺍﻟﻤﺎء ﻋﻠﻰ ﺳﻄﺢ‬
‫ﺍﻷﺟﺎﺭ ‪،‬ﻭﺫﻟﻚ ﻟﺘﺠﻨﺐ ﺗﻜﻮﻥ ﻓﻘﺎﻋﺎﺕ ﺍﻟﻬﻮﺍء ﺑﻴﻦ ﺍﻟﻤﺮﺷﺢ ﻭﺳﻄﺢ‬
‫ﺍﻵﺟﺎﺭ‪.‬‬
‫ﻋﻨﺪﺩﺭﺟﺔ ﺣﺮﺍﺭﺓ ‪ 0.2 ± 44.5‬ﺩﺭﺟﺔ ﻣﺉﻮﻳﺔ ﻟﻤﺪﺓ ‪ 2 ± 24‬ﺳﺎﻋﺔ‪.‬‬
‫ﺩﻳﻔﻜﻮ™ﻣﺮﻕ ﺇﻑ ﺳﻲ‬
‫‪.1‬ﺗﺤﻀﻴﺮ ﻭﺳﻂ ﺍﻟﻤﺮﻕ ﻣﻦ ﺍﻟﻘﺎﻋﺪﺓ ﺍﻟﻤﺠﻔﻔﺔ ﻭﻓﻘﺎً ﻟﺘﻮﺟﻴﻬﺎﺕ‬
‫ﺍﻟﻤﻠﺼﻖﻭﺇﺿﺎﻓﺔ ﻣﺤﻠﻮﻝ ﺣﻤﺾ ﺍﻟﻮﺭﺩ‪.‬‬

‫‪.5‬ﺑﻮﺭﺩﻧﺮ ‪ ،‬ﻭﻳﻨﺘﺮ ﻭﺳﻜﺎﺭﺑﻴﻨﻮ )ﻣﺤﺮﺭ(‪ .1978 .‬ﺍﻟﻄﺮﻕ ﺍﻟﻤﻴﻜﺮﻭﺑﻴﻮﻟﻮﺟﻴﺔ ﻟﺮﺻﺪ ﺍﻟﺒﻴﺉﺔ‪ :‬ﺍﻟﻤﻴﺎﻩ ﻭﺍﻟﻨﻔﺎﻳﺎﺕ‪ .‬ﺍﻟﻤﻨﺸﻮﺭ‬
‫‪ .EPA-600 / 8-78-017‬ﻣﻌﻤﻞ ﺍﻟﺮﺻﺪ ﻭﺍﻟﺪﻋﻢ ﺍﻟﺒﻴﺉﻲ ‪ ،‬ﻣﻜﺘﺐ ﺍﻟﺒﺤﺚ ﻭﺍﻟﺘﻄﻮﻳﺮ ‪ ،‬ﻭﻛﺎﻟﺔ ﺣﻤﺎﻳﺔ ﺍﻟﺒﻴﺉﺔ‬ ‫‪.2‬ﺃﺿﻒ ‪ 2‬ﻣﻞ ﻣﻦ ﺍﻟﻤﺮﻕ ﺍﻟﻤﺒﺮﺩ ﺇﻟﻰ ﺿﻤﺎﺩﺍﺕ ﻣﺎﺻﺔ ﻣﻌﻘﻤﺔ ﻓﻲ‬
‫ﺍﻷﻣﺮﻳﻜﻴﺔ ‪،‬ﺳﻴﻨﺴﻴﻨﺎﺗﻲ ‪ ،‬ﺃﻭﻫﺎﻳﻮ‪.‬‬
‫‪.6‬ﺇﻳﺘﻮﻥ ﻭﻛﻠﻴﺴﻴﺮﻱ ﻭﻏﺮﻳﻨﺒﺮﻍ )ﻣﺤﺮﺭ(‪ .1995 .‬ﺍﻟﻄﺮﻕ ﺍﻟﻘﻴﺎﺳﻴﺔ ﻟﻔﺤﺺ ﺍﻟﻤﻴﺎﻩ ﻭﺍﻟﺼﺮﻑ ﺍﻟﺼﺤﻲ ‪ ،‬ﺍﻟﻄﺒﻌﺔ ‪ .19‬ﺍﻟﺮﺍﺑﻄﺔ‬ ‫ﺃﻃﺒﺎﻕﺑﻼﺳﺘﻴﻜﻴﺔ ﺧﺎﺻﺔ ﻣﺤﻜﻤﺔ ﺍﻟﻐﻠﻖ‪.‬‬
‫ﺍﻷﻣﺮﻳﻜﻴﺔﻟﻠﺼﺤﺔ ﺍﻟﻌﺎﻣﺔ ‪ ،‬ﻭﺍﺷﻨﻄﻦ ﺍﻟﻌﺎﺻﻤﺔ‬
‫‪.3‬ﻗﻢ ﺑﻠﻒ ﻣﺮﺷﺢ ﺍﻟﻐﺸﺎء ﺍﻟﻤﺴﺘﺨﺪﻡ ﻟﺘﺠﻤﻴﻊ ﻋﻴﻨﺔ ﺍﻟﻤﺎء ﻋﻠﻰ‬
‫ﺍﻟﻀﻤﺎﺩﺓﺍﻟﻤﺎﺻﺔ ﺍﻟﻤﺒﻠﻠﺔ ‪ ،‬ﻭﺫﻟﻚ ﻟﺘﺠﻨﺐ ﺗﻜﻮﻥ ﻓﻘﺎﻋﺎﺕ ﺍﻟﻬﻮﺍء‬
‫ﺑﻴﻦﺍﻟﻤﺮﺷﺢ ﻭﺍﻟﻮﺳﺎﺩﺓ‪.‬‬
‫‪.4‬ﺿﻊ ﺍﻷﻃﺒﺎﻕ ﻓﻲ ﺃﻛﻴﺎﺱ ﺑﻼﺳﺘﻴﻜﻴﺔ ﻭﺍﺣﻀﻨﻬﺎ ‪ ،‬ﻋﻦ ﻃﺮﻳﻖ ﺍﻟﻐﻤﺮ ‪ ،‬ﻓﻲ ﺣﻤﺎﻡ ﻣﺎﺉﻲ‬
‫ﻋﻨﺪﺩﺭﺟﺔ ﺣﺮﺍﺭﺓ ‪ 0.2 ± 44.5‬ﺩﺭﺟﺔ ﻣﺉﻮﻳﺔ ﻟﻤﺪﺓ ‪ 2 ± 24‬ﺳﺎﻋﺔ‪.‬‬

‫ﺍﻹﺻﺪﺍﺭﺍﻟﺜﺎﻧﻲ ‪Difco ™ & BBL ™ Manual ،‬‬

 ‫ﺩﻳﻔﻜﻮ™‪m FC Broth Base‬‬ ‫ﺍﻟﺘﻮﻓﺮ‬
‫ﻭﻛﺎﻟﺔﺣﻤﺎﻳﺔ ﺍﻟﺒﻴﺉﺔ ‪SMWW‬‬
‫ﺩﻳﻔﻜﻮ™ﻡ ‪FC Agar‬‬
‫ﻣﺠﻔﻔﺔ‪ 100 -‬ﻍ ﻣﺠﻔﻔﺔ‬ ‫ﻗﻄﺔ‪.‬ﺭﻗﻢ ‪288320‬‬ ‫‪AOAC CCAM EPA SMWW‬‬
‫‪ 500-‬ﻍ‬ ‫‪288330‬‬
‫ﻣﺠﻔﻔﺔ‪ 100 -‬ﻍ ﻣﺠﻔﻔﺔ‬ ‫ﻗﻄﺔ‪.‬ﺭﻗﻢ ‪267710‬‬
‫ﺩﻳﻔﻜﻮ™ﺣﻤﺾ ﺭﻭﺯﻭﻟﻴﻚ‬ ‫‪ 500-‬ﻍ‬ ‫‪267720‬‬
‫ﻗﻄﺔ‪.‬ﺭﻗﻢ ‪ 232281‬ﻗﺎﺭﻭﺭﺓ ‪ 1×6 -‬ﺟﺮﺍﻡ‬

‫ﺍﻹﺻﺪﺍﺭﺍﻟﺜﺎﻧﻲ ‪Difco ™ & BBL ™ Manual ،‬‬