The Effect of Temperature on 7/9 Bacteria Growth in Milk Purpose The purpose of this lab is to determine if temperature has an effect on the growth of bacteria in milk. Hypothesis he ‘MG hypothesis states-that temperature will have an effect on the growth of bacteria in milk. It is believed that the milk placed in a warm place will have more bacteria growth than the milk placed in a refrigerator. Background If the temperature of milk increases and then rapidly decreases bacteria can start to form in it. Also if milk gets to warm it can spoil and curdle towards the bottom of the cup. The curdling, is usually due to the lactose in milk. Procedure Materials: 1) Milk 2) Two eight ounce cups 3) Cling wrap 4) A thermometerProcess Step I: Place two eight ounce cups on a solid, flat surface. 3: Place cling wrap securely over cup. Step 4: Place one of the cups in a refrigerator. Step 5: Place the other cup in a warm place. Step 6: Come back in one day and remove the cling wrap from both cups. Step 7: Record any bacteria growth such as lumps or smells. Step 8: After recording the bacteria growth place thermometer in the cup that has been refrigerated and record the temperature. Step 9: Then place thermometer in cup that has not been refrigerated and record the temperature. Step 10: Place cling wrap back on cups. Step 11: Place refrigerated cup back in refrigerator. Step 12: Place cup that was in a warm place back in a warm place. Step 13: Repeat steps 6-12 for the next seven days until the research is done. Results (11/2 1/28) Quantitative Results: With the refrigerated milk I found there was only a faint smell on the last day otherwise there was no smell. However, within the first day of the experiment with the warm place milk, there was a distinct smell and as the week went on it ‘got worse and it formed some sort of material at the top that started to bubble. Conclusion The original hypothesis stated that temperature would have an effect on the growth of bacteria. It also stated that the cup placed in a warm place would have more bacteria growth than therefrigerated cup of milk. After the experiment was conducted, it ‘was concluded that temperature does, in fact, have an effect on bacteria growth. As the second day of the experiment approached, the warm place milk already had a smell and by the third day Jumps had already formed in the milk. This shows that warmer temperatures will cause more bacteria to grow in glasses of milk. In this activity, it was learned that if milk is not refrigerated, bacteria will grow more rapidly then in refrigerated milk. In the refrigerated milk, there was no smell throughout the experiment, until the last day. However, in the warm place milk, the milk smelled the second day. As the week of experimenting went on, Jumps appeared and began to grow and multiply. By the end of the experiment, there was a floating substance of lumps and bubbles at the top, and a clear liquid underneath. This suggests that non- refrigerated milk increases the growth of bacteria in milk, compared to refrigerated milk. In this lab everything went as originally planned. The procedures were followed correctly and the correct data was able to be collected. However, some unplanned variables could include the time of day the data was collected, if the milk was directly under a light and if the temperature was taken from the refrigerated milk first. The time of day the data was collected could be an unplanned variable because if the refrigerator or air conditioning was on a cooling cycle it could affect the temperature of the milk causing the data to change periodically. If the warm place milk was directly under a light it would be an unplanned variable because it could increase the temperature of the milk. Also if the temperature of the refrigerated milk was taken first it could cool down the tip of the thermometer causing an inaccurate reading of the warm place milk. Some similar experiments could be conducted by changing a variable, One of these could be: “Does bacteria grow faster in low fat milk or fat free milk?” The variable that would have been changed would have been to make one glass of milk low fat and the other fat free. However, in such an experiment the two milkcontainers would need to be kept at the same temperature. Another experiment that could be conducted by changing a variable could be: “Does the size of the container that the milk is in effect the amount of the bacteria that grows in the milk?” Here the variable that would have been changed would have been that there would be two different sized containers for the milk. This lab could relate to a real life situation because it shows that bacteria grows better on non-reftigerated milk products. This, ‘would show that itis better for people’s health to keep their milk products in a refrigerator. If milk is kept outside of a reftigerator, when it is drunk, it could cause the person who drinks it to become very sick. This would happen because all that bacteria that was on the milk would then be transferred into that person’s body. 1 enjoyed this lab very much. One reason I enjoyed it so much is because it was fun to see how lumps would form in the milk. Another reason I enjoyed so much is that it was not just some random experiment, I enjoyed that it could actually be related to something that could happen in every day life.YY rau FEY PISO TOOL 7D} 0, WTS AP W f Ee rae | oy eae(setr [Sow [ser osor [J.or [Josey (Jcov | Joe | ww peleeBeu—— su Ath ae [sec Acts deste dete — Joge | mv enrg EM——— MON NONSE PONTE MONEE HONZE RONAZ a A a ee Halyuaey seeibaq ateeie@ar siosecg <2 = MIN 84} Jo eAnjesodwie, EYL