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Restriction Enzymes 2023
Restriction Enzymes 2023
ATP is required
Restriction Enzymes
There are already more than 1200 type II enzymes isolated
from prokaryotic organism
They recognize more than 130 different nucleotide sequence
They scan a DNA molecule, stopping only when it recognizes a
specific sequence of nucleotides that are composed of
symetrical, palindromic sequence
Palindromic sequence:
The sequence read forward on one DNA strand is
identical to the sequence read in the opposite direction
on the complementary strand
G A T C C
G
G
C C A G G
Restriction Product
Restriction enzymes