PROCEDURE
MIDSTREAM CLEAN CATCH METHOD:
1. Ask the woman to wash her hands.
2. She then needs to open the commercial
clean-catch urine specimen kit and moisten
the cotton balls with the antiseptic solution
or open the prepared antiseptic wipes.
3. Ask the woman to sit on the commode and
separate her labia with her nondominant
hand.
4. She should then cleanse her perineum,
washing from front to back, using a cotton
ball or wipe for only one stroke and then
discarding it.
5. Caution her to avoid touching the
inside of the container or cap
6. The woman should begin urinating, allowing
the first urine to flow into the toilet. Next,
she should hold the container under the
urine stream until approximately 10 to 20 ml
has been obtained. Once the specimen is
obtained, she can remove the container,
release her hand from her labia, and finish
voiding into the toilet.
7. To finish, she should cap the specimen
container, wash her hands, and bring the
specimen to you. Encourage her to report
whether she felt any pain on urination.
BENEDICT’S TEST
1. Assemble the necessary equipment.
2. Collect a midstream clean catch urine
3. Take 5 ml of Benedict’s solution into the test
tube
4. Add 8-10 drops of urine into the Benedict’s
solution
5. Now fix the test tube holder, bring the test
tube near the Bunsen burner and allow it to
heat for 2 minutes.
6. While it is heating, keep stirring the tube
continuously.
7. Observe the changes
RATIONALE
To prevent contamination of the urine and to
prevent spread of infection.
Prepare the equipment needed. To avoid
consuming the time.
To easily catch the urine sample.
Front-Back practices the principle: cleanest to
dirtiest. To prevent contamination of the urine
sample.
Maintain sterile technique to prevent
contamination which may alter the
result.
This reduces the risk of the sample being
contaminated with bacteria from: your hands
and the skin around the urethra, the tube that
carries urine out of the body. Adequate volume
of the urine sample (approx. 10-20ml) is also
important to further examine the urine. Not
enough urine sample, may result to difficulty of
assessing and examining the sample.
Be sure to put the correct label (name, age,
gender) of the container before sending it to
the laboratory for testing. Further assessment of
the elimination pattern, include presence of pain
on urination which may indicate UTI.
To avoid consuming the time.
Using midstream clean catch method will
prevent harboring bacteria from the
genitals of the patient.
Using the pipette(dropper), take 5ml of the
solution to the test tube. Benedict’s solution is
used to test for the presence of sugar in the
urine.
Urine was used as a type of clinical sample.
Presence of glucose in the urine may indicate
Glycosuria. It typically occurs due to high blood
sugar levels or kidney damage.
Some sugars such as glucose are called
reducing sugars. When reducing sugars are
mixed with Benedicts reagent and heated, a
reduction reaction causes the Benedicts reagent
to change color. The color varies from green to
dark red (brick) or rusty-brown, depending on
the amount of and type of sugar.
Wait until it boils or changes it color to further
see the result.
 If the color upon boiling is changed into green,
then there would be 0.1 to 0.5 percent sugar
in solution.
• If it changes color to yellow, then 0.5 to 1
percent sugar is present.
• If it changes to orange, then it means that 1
to 1.5 percent sugar is present.
• If color changes to red, then 1.5 to 2.0
percent sugar is present.
• And if color changes to brick red, it means
that more than 2 percent sugar is present in
solution.