MOLECULAR IMMUNOLOGY

(VAI-311)

Hussain Mustatab Wahedi, PhD Lecture 15

Department of Healthcare Biotechnology 03-May-2023
ASAB, NUST, Islamabad SP-2023
 History?

• First Cell Culture dates back to early 20th century

• Nerve fiber growth in 1907
• 1940s – 1950s → Industrial scale cell culture
• Polio Vaccines → 1st product using cell culture

2
 What is Cell Culture? Why?

Process to grow animal/plant/insect cells in artificial but

favorable environment.

• Basic Cell Biology/Mechanisms/Cell-Cell or Cell-Matrix interaction

3
 What is Cell Culture? Why?

• Gene therapy for replacing non-functional/defected

genes

4
 What is Cell Culture? Why?

• Efficacy and toxicity testing of new drugs

5
 What is Cell Culture? Why?

• Characterization of Cancer Cells

6
 What is Cell Culture? Why?

• Production of vaccines, mABs, pharmaceutical drugs

7
 What is Cell Culture? Why?

• Production of viruses for use in vaccine development

8
 Sources of Ex-Vivo Culture?
ORGAN CULTURE
❖ Adult organs
❖ Retain differentiation
❖ Growth is in peripheries
❖ Functional Properties
❖ Effects of drugs/hormones
❖ Fresh explant for every exp.
Reproducibility?
PRIMARY EXPLANT
❖ Fragments from organs
❖ Tissue adheres ECM Component
❖ Migration from peripheries → outgrowth
❖ Growth characteristics of Cancer cells
CELL CULTURE
9
 Sources of Cell Culture?

Cell CULTURE

❖ Cells from the ex-plant

❖ Free floating cells
Histotypic Cultures
❖ Enzymatic/Mechanical means
- Appropriate ECM & factors
❖ Monolayer/suspension
- High cell density
(Anchorage dependent/independent)
❖ Reconstruction of 3D Structures 1) Filter-mediated
2) Agarose/agar/spheroids
3) Grown on Hollow fiber

Organotypic Cultures
Cell Line/Cell Strain
- Heterotypic cell interaction
- Co-culture → functionality
10