SUMMATIVE TEST IN BIOTECHNOLOGY 10. Which of the following statement is TRUE?

NAME: _____________________ SCORE:_______ A. DNA is positively charged

SECTION:___________________ B. Ligase is used to isolate the genetic material.
C. Thermal Cycler cuts fragment of DNA and the
Directions: Read each question carefully. Choose the letter of vector.
the correct answer. D. Gel Electrophoresis separates the DNA Molecule
according to size.
1. What molecule is being used to cut a specific area of
the DNA?
11. What organism is being used to transfer foreign genetic
A. Agarose Gel C. Recombinant Cells
material into a cell?
B. Plasmid D. Restriction enzymes
A. DNA C. Restriction enzymes
B. Plasmid D. Vector
2. In what step does the cut fragment of DNA and the cute
vector are joined together?
A. Insertion of Recombinant DNA into Host 12. Which enzyme is being used to amplify the DNA?
B. Isolation of Recombinant Cells A. Endonuclease C. DNA Ligase
C. Obtaining or culturing the Foreign Gene product B. DNA Helicase D. DNA Polymerase
D. Amplification Using PCR

3. The recombinant DNA is introduced into a recipient host 13. Which among the following does NOT describe the
cell, mostly a bacterial cell, which step is it? principle of Gel Electrophoresis?
A. Insertion of Recombinant DNA into Host A. DNA fragments are separated on the basis of size.
B. Isolation of Recombinant Cells B. The DNA fragments will move towards th
C. Obtaining or culturing the Foreign Gene product negative charge.
D. Amplification Using PCR C. The smallest DNA fragments will move faster
than the larger DNA fragments.
4. What is NOT a vital role of recombinant DNA technology D. DNA fragments are injected into wells and an
nowadays? electric current is applied along with the gel.
A. Improve health conditions by developing new
vaccines and pharmaceuticals. 14. What equipment is used to amplify the DNA?
B. Improve by developing diagnostics kits, monitoring A. Centrifuge Machine C. Pipette
devices and new therapeutic approaches. B. Microscope D. Thermal Cycler
C. Synthesis of synthetic human insulin and
erythropoietin by genetically modified Bacteria 15. Which among the following serves as a starting point
D. Production of new types of experimental mutant for DNA synthesis?
cat for research purposes. A. DNA polymerase C. Restriction enzymes
B. Primer D. Tag polymerase

5. Which among the following is the Blueprint of Life? 16. Which is NOT true about Nuclear cloning?
A. Cell C. Protein A. introduction of the nucleus from a cell into an
B. DNA D. RNA enucleated egg cell
B. can use bacteria to make multiple copy
6. What steps of genetic recombination technology C. somatic cell
happens the separation and D. clone organism has a sign that it is clone
purifying which involves enzymes such as restriction
enzymes? 17. How does human antithrombin produce modified
A. Restriction Enzyme Digestion goats helps human?
B. Isolation of Genetic Material A. It is used to treat a rare blood clotting
C. Amplification Using PCR disorder in humans
D. Ligation of DNA Molecules B. It is used to treat diabetes that is common to
humans.
7. The new hybrid DNA molecules isa also called a C. It helps to reduce the risk in allergy.
recombinant DNA molecule and the technology is referred D. It helps human to cure anemia .
to as ____________________________.
A. Recombinant Engineering Technology 18. Which refers to the combination of two DNA strands that
B. Recombinant Science and Technology are constructed artificially?
C. Recombinant DNA Technology A. Genetic Material C. Recombinant DNA
D. Recombinant RNA Technology B. Recombinant Cells D. Restriction Enzymes

8. The following statements are all TRUE EXCEPT.

A. The DNA is located in the nucleus of the cell. 19. Why do you do you think producing Dolly is an important
B. Recombinant DNA is a combination of two different breakthrough in Science
strands of DNA. and Technology?
C. DNA Ligase is used to amplify a single copy or a A. Dolly was remarkable in being the first mammal
few copies of DNA. to be cloned from an adult cell.
D. In Agarose Gel Electrophoresis the negatively B. Dolly was remarkable in being the first avian to be
charged DNA travels to the positive electrode and cloned from an adult cell.
is separated out based on size. C. Dolly was remarkable in being the first reptilian to be
cloned from an adult cell.
9. Which of the following refers to chemically synthesized D. Dolly was remarkable in being the first mammal to be
oligonucleotides that are complementary to a region of cloned from an young cell.
the DNA?
A. Agarose Gel C. Restriction Enzymes 20. What was the first sign that showed Dolly was cloned?
B. Primers D. Vectors A. Dolly’s black eyes C. Dolly’s white face
B. Dolly’s black face `` D. Dolly’s long arm

21. Who made Dolly the Sheep?

A. Hamilton Smith C. Kary Mullis
B. Hans Spemman D. Ian Wilmut
22. What are the steps of cloning a gene in a bacterial
plasmid?
I. Isolation of vector and gene-source DNA.
II. Insertion of DNA into the vector.
III. Introduction of the cloning vector into cells
IV. Cloning of cells (and foreign genes).
V. Identifying cell clones with the right gene
A. I,II, III, IV & V C. IV, V, I, II, & III
B. V, IV, II, III, & I D. V, IV, III, II, & I
32. Are foods from genetically modified crops nutritionally superior?
A. Yes, they offer some health advantages over foods produced
from conventional crops.
B. Yes, they offer substantial health advantages over foods
produced from conventional crops.
C. No, they are neither better nor worse than foods from
conventional crops.
D. No, they are slightly less healthful than foods from
conventional crops.

23. Which is the final steps of cloning a gene, where 33. Genetic Engineering has been successfully used for producing
sorting through a thousands of bacterial colonies _______________.
with foreign DNA to find those containing gene A. Transgenic mice for testing safety of polio vaccine before
interest? used in humans.
A. Cloning of cells B. Transgenic models for studying new treatments for certain
B. Identifying cells clones with right gene cardiac diseases.
C. Insertion of DNA into vector C. Transgenic cow- Rosie which produces high fat milk for
D. Introduction of the cloning vector into cells making ghee
D. Animals like bulls for farm work as they have super power.

24. When was the first nuclear cloning conceived? 34.Which of the following is not an example of an advantage of
A. 1928 C. 1930 genetic engineering?
B. 1929 D. 1931 A. farmers may need to use less pesticides
B. modified cotton, corn and soybeans help create
“superweeds”
25. Antithrombin (ATryn), an anticoagulant used for the C. can improve nutrition and taste of some foods
prevention of blood clots with a D. may have a greater resistance to spoilage
rare disease. Which animals was genetically modified to
produce FDA-approved human 35. Used to transport desired genes or pieces of DNA from one
antithrombin (ATryn)? organism into a bacterial cell?
A. Cat C. Goat A. Plasmid C. Electrophoresis Gel
B. Frog D. Sheep B. Restriction Enzyme D. Polymerase Chain
Reaction
26. Golden rice is a new type of rice that contains beta
carotene, what vitamin is present 36. When was the Polymerase Chain Reaction discovered?
in Golden rice? A. 1963 C. 1983
A. Vitamin A C. Vitamin C B. 1973 D. 1993
B. Vitamin B D. Vitamin D
37. Genetically modified organisms increase crop yield, what does
27. What process where mice are used to study gene this lead to?
function by purposely turning off specific genes?. A. greater availability to food
A. Apoptosis C. Gene knockout B. death of more crops
B. Cell knockout D. Genetosis C. to malnutrition
D. decrease of supply of foods
28. Which genetic engineering process contains foreign DNA
that has been introduced 38. What are the current benefits of having foods made from
using biotechnology? genetically modified crops?
A. Gene cloning C. GMO A. They allow farmers to greatly increase the number of crops
B. Nuclear cloning D. Transgenic organism produced.
B. They improve convenience for consumers, e.g. by
creating foods with longer shelf lives.
29. Who developed the process of nuclear cloning? C. They improve farm profitability and make some farmer’s jobs
A. Hamilton Smith C. Hindll easier.
B. Hans Spemman D. Kary Mullis D. They improve the nutritional quality of foods.

39. Food crops like soy and corn that have been
30. Which best describes genetic manipulation?
genetically modified for pest and herbicide resistance.
A. A mechanism by which each time the host
What do you call an organism that has been contains
cell reproduces; several copies of a particular
genes from two different organisms?
gene are produced wherein it is possible to
A. Altered genome organism
clone whole species.
B. Mutant organism
B. The insertion of a fragment of DNA carrying
C. Modified Organism
a gene into a cloning vector and
D. Transgenic Organism
subsequent propagation of recombinant
DNA molecules into many copies
40. Biotechnology uses as procedure called genetic
C. The introduction of the nucleus from a cell
engineering, which of the following
into an enucleated egg cell
best describes the use of genetic engineering?
D. The process of inducing changes in
A. alter the genetic makeup of an organism
gene expression and expression of
B. generate hew hybrid strains
novel genes and has proven to be an
C. improve disease resistance
indispensable tool in genetic research.
D. all of the above
31. This was the name given to the first mouse used to
study cancer.
A. Mighty mouse C. Oncomouse
B. Mousegenic D. Supermouse
41. Which of the following is the most challenging issue
facing genome sequencing or organism
modification?
A. the availability and stability of DNA
B. the ethics of using information from genomes at
the individual level
C. the inability to develop fast and accurate
sequencing or modifying techniques
D. all of the above

42. The development of Polymerase Chain Reaction has

been a major breakthrough in scientific world, who
among the following discovered PCR?
A. Hamilton Smith C. Kary Mullis
B. Hindll D. Werner Abner

43. Kenneth is part of the SOCO investigators, he study and

investigate the genomic information from trace evidence
found at crime scenes, what branch of biotechnology
operates in that field?
A. Biotechnology C. Industrial biotechnology
B. Forensics biotechnology D. Medical biotechnology

44. What is a technique commonly used in laboratories to separate

charged molecules like DNA RNA and proteins according to
their size?
A. Gel Electrophoresis
B. Molecular Scissor
C. Polymerase Chain Reaction
D. Selection of Prokaryotic Host

45. Plasmid naturally exist in bacterial cells and they also

occur in some eukaryotes,
which bacterium was first time used in recombinant DNA
technology?
A. E. coli C. S. cerevisiae
B. E. faecalis D. S. typhimurium

46. This is a laboratory technique used to amplify a single

copy or a few copies of a segment of DNA across
several orders of magnitude, generating thousands to
millions of copies of a particular DNA sequence.
A. Agarose Gel Electrophoresis
B. Molecular Scissor
C. Polymerase Chain Reaction
D. Restriction Endonuclease

47. The enzymes that make one incision on each of the

two strands of DNA at specific locations based on the
nucleotide sequence are known as_______.
A.DNA Polymerase C. Primase
B. Helicase D. Restriction Endonuclease

48. The term is defined as the basic physical and

functional unit of heredity.
A. alleles C. genes
B. deoxyribonucleic acid D. ribonucleic acid

49. It is a branch of science that is concerned how

various traits are inherited.
A. biological engineering C. heredity
B. genetics D. pathology

50. Recombinant DNA technology is utilized to alter the

genetic makeup of an organism, what process is it?
A. biological engineering C. genetic engineering
B. biological modification D. genetic modification

51. When did Hindll isolate the first restriction enzyme?

A. 1960 C. 1990
B. 1980 D. 1970