A small drop of blood was placed on a slide and spread evenly behind another slide at a 30-40 degree angle. The second slide was then pushed smoothly and quickly to the left, smearing the blood about half the length of the slide. It is best to make multiple smears with a single blood drop to obtain one with a good cell distribution without an excessive amount of blood hiding the leukocytes.
A small drop of blood was placed on a slide and spread evenly behind another slide at a 30-40 degree angle. The second slide was then pushed smoothly and quickly to the left, smearing the blood about half the length of the slide. It is best to make multiple smears with a single blood drop to obtain one with a good cell distribution without an excessive amount of blood hiding the leukocytes.
A small drop of blood was placed on a slide and spread evenly behind another slide at a 30-40 degree angle. The second slide was then pushed smoothly and quickly to the left, smearing the blood about half the length of the slide. It is best to make multiple smears with a single blood drop to obtain one with a good cell distribution without an excessive amount of blood hiding the leukocytes.
A small drop of blood was placed on a slide and spread evenly behind another slide at a 30-40 degree angle. The second slide was then pushed smoothly and quickly to the left, smearing the blood about half the length of the slide. It is best to make multiple smears with a single blood drop to obtain one with a good cell distribution without an excessive amount of blood hiding the leukocytes.
another slide was brought in contact with the drop and the drop was allowed to bank evenly behind the spreader. The angle between the two slides must be 30-40 degrees. Now, push to the left in a smooth, quick motion. The smear was covered about half the slide.
Notes:
▪ It is important that the quantity of blood is not
excessive; otherwise, the red cells could hide the leukocytes. A gradual transition was made from thick to thin in a smear and a zone was obtained with a good cell distribution.
▪ With a single drop of blood, several smears were
made. In fact, it is sufficient to leave a blood spot on the slide that is about 3 mm in diameter to create a smear. It is useful to perform many smears. In fact, not always they are successful, and with some attempts, it is easier to get one well prepared. To avoid producing clots, each smear was made with fresh blood and straight after having deposited it.
▪ When the blood was deposited by one person and the
smears were formed by another, it is helpful to have assistance for this purpose. With the microscope, the smears were observed to check that some of them are properly made. The red cells did not overlap one another or are too few to spread out.