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Trends in Food Science & Technology 133 (2023) 231–243

Contents lists available at ScienceDirect

Trends in Food Science & Technology


journal homepage: www.elsevier.com/locate/tifs

The research progress of physical regulation techniques in 3D food printing


Yinqiao Lv, Weiqiao Lv *, Guohua Li, Yuanliang Zhong
College of Engineering, China Agricultural University, Beijing, 100083, China

A R T I C L E I N F O A B S T R A C T

Handling Editor: AR Jambrak Background: With the increasing application of three-dimensional printing, (3D printing) in the modern food
processing industry, research in this area has made great progress. Recently, the application of physical regu­
Keywords: lation techniques in 3D food printing has been of great interest to researchers to improve printing quality.
3D food printing Scope and approach: This review summarizes the various physical methods used in the pre-, in-process, and post-
Energy-carrying electromagnetic field
treatment of 3D food printing. Further, the application of energy-carrying electromagnetic fields, ultrasonic, and
Electrothermal regulation
electric heating in the whole process of 3D food printing is highlighted. Their mechanisms of action and treat­
Printing mode
ment effects on printing substrates with different properties are also described.
Key Findings and Conclusions: Different from pre-printing methods such as expanding the range of printing
substrates and adding functional materials to improve printing characteristics, physical methods such as energy-
carrying electromagnetic fields, electrothermal regulation, and forming modes can affect the final quality of the
printed product. This can be from printing substrate preparation to post-treatment, and expansion to four-
dimensional printing, (4D printing). Successful printing requires a reasonable selection and combination of
various physical regulation techniques. This helps to improve substrate printing characteristics based on different
material properties and desired results to obtain high-quality printed products, according to this review.

1. Introduction food printing more flexible and diverse, thereby enriching the content of
personalized as well as customized food. Currently, there are four main
Three-dimensional printing is a new manufacturing technology that forming modes of 3D food printing that include extrusion based, selec­
uses computer-aided design software and digital processing equipment tive laser sintering, binder jetting, and inkjet. Of the four methods, the
to produce stereoscopic products. This technology is now widely used in extrusion-based printing is the most widely used. According to the
the automotive, aerospace, pharmaceutical, and food industries. In the different states of printing substrates, it can be divided into room tem­
food processing field, much of the relevant research has focused on perature, hot-melt, and hydrogel-forming extrusion.
expanding the range of ingredients for printing and improving the The 3D food printing technology is a cutting-edge processing tech­
substrate characteristics, thereof. Ingredients applied to 3D printing are nique that integrates various disciplines such as food, mechanics, com­
generally a powder, paste, or melted materials at room temperature (Lin puters, and electromagnetics (Q. Jiang, Zhang, & Mujumdar, 2021;
Wang, Zhang, Bhandari, & Yang, 2018). For example, starch (S Than­ Zheng, Zhang, & Liu, 2021). The printing effect is influenced, not only
galakshmi & Arora, 2021), plant fiber (Pant et al., 2021), isolated pro­ by the substrate components and product shape, but also by a combi­
tein (J. Chen et al., 2019), and chocolate (Mantihal, Prakash, Godoi, & nation of physical factors such as printing temperature, forming mode,
Bhandari, 2017) have become the main substrates of 3D food printing, homogenization, and energy-carrying electromagnetic fields. In 3D food
thereby providing a good carrier for stereoscopic reproduction to ach­ printing, according to the substrate properties and the target effect, the
ieve various customized functions. The material performance and appropriate forming mode is selected to obtain the desired stereoscopic
properties of common printing substrates are shown in Table 1. A variety product. Temperature has an important influence on the rheological
of thickeners (Cai, Zhong, Xu, Huang, & Lu, 2022; C. Severini, Derossi, properties, extrusion state, and forming effect of the substrates during
Ricci, Caporizzi, & Fiore, 2018; Vancauwenberghe et al., 2017), nutrient the printing process. Proper temperature control is one of the necessary
supplements (Carla Severini & Derossi, 2016; Zhang et al., 2022), and factors for successful printing. Energy-carrying electromagnetic field
coloring agents (Goyanes et al., 2019; Wen, Kim, & Park, 2022) make 3D treatment can improve the physicochemical and functional properties of

* Corresponding author.
E-mail address: lvweiqiao@cau.edu.cn (W. Lv).

https://doi.org/10.1016/j.tifs.2023.02.004
Received 5 December 2022; Received in revised form 24 January 2023; Accepted 5 February 2023
Available online 7 February 2023
0924-2244/© 2023 Elsevier Ltd. All rights reserved.
Y. Lv et al. Trends in Food Science & Technology 133 (2023) 231–243

food substrates (Li et al., 2021), thereby making targeted extensions to

(Lee, Won, Kim, & Park, 2019; J.


(Bianca C. Maniglia et al., 2020;
3D printing in time and space (Phuhongsung, Zhang, & Bhandari, 2020).

(Fan et al., 2020; Phuhongsung,


Chen, 2021; Y. Liu et al., 2022)
(S. Ma, Schutyser, et al., 2022)
Arora, & Liu, 2022; Villanueva
It is applied to 3D printing pre, in-process, and post-treatment (He,

Zhang, & Devahastin, 2020)


(Prithviraj, Thangalakshmi,

(H.-z. Chen, Zhang, Guo, &


B. C. Maniglia et al., 2019)

(S. Ji, Xu, Li, et al., 2022;


Zhang, & Fang, 2020), and the properties of printing substrates are

Maniglia et al., 2021)


greatly improved by the exposure to different energy-carrying electro­
magnetic fields. Using energy-carrying electromagnetic field regulation

Sun et al., 2015)


techniques to improve the texture and efficiency of printed food has

et al., 2019)
resulted in more innovative research results. This helps to provide a new
Reference

direction for research that is aimed at improving the precision, quality,


and productivity of 3D food printed products. Additionally, physical
regulation methods such as energy-carrying electromagnetic fields can
The pulsed electric fields treatment leads to fractures on granules surface,
The MW treatment of rice flours resulted in substantial changes in dough

The appropriate ultrasound-assisted thawing treatment did not cause high

The protein is gradually unfolded and cross-linked by microwave heating


to form a gel, and the rheological properties and structural strength of the
Gels made from electrothermally treated cassava starch have lower peak

also expand 3D into four-dimensional printing (4D printing), or even


viscoelastic and pasting properties. This contributes to improved bread-

thawing loss. There is no significant differences were observed for flow


starch surface and reduced the amylose content, viscosity and pasting
The radio frequency electromagnetic wave treatment roughened the

higher capacities (Ghazal, Zhang, Mujumdar, & Ghamry, 2022; He,

standardized ink quality and result in desired printing performance


and viscoelastic properties at room temperature among surimi gels

Materials crushed into appropriate size particles can maintain the


Zhang, & Guo, 2020).
Currently, many physical factors are applied to enhance the quality
of 3D food printing. Although specific regulation techniques and treat­
ment conditions have been proposed, the role or even the combined
reduction in peak apparent viscosity, and firmer gels

effect of physical regulation at different stages of printing has not been


systematically elaborated. This review describes the application of
apparent viscosity and increased gel firmness

different physical regulation techniques before, during, and after


printing. It expounds the effects and principles of physical factors on the
printing properties and printing results.
Effect of physical treatments

2. The effect of pre-printing physical regulation on 3D food


temperature of the starch

printing results
making performance

ink are improved

The 3D food printing pretreatment has an important impact on the


printability of the substrate, and the optimization achieved by pre­
treatment can be characterized by printability assessment techniques. In
many studies, functional materials have been added to printing sub­
strates with the aim of improving their printing performance (S. Ji, Xu,
Liu, et al., 2022; Yu, Wang, Li, Wang, & Wang, 2022). In recent years,
electromagnetic wave

physical regulation techniques such as temperature, energy-carrying


electromagnetic fields and ultrasound have also been increasingly
Radio frequency
Electrothermal
Pre-treatment

used in 3D food printing pretreatment.


Comminution
Electric fields
Microwave

Ultrasound

Microwave
technique

treatment

2.1. The printability assessment of physically treated substrates

Printability is a key factor in judging the success of the physical


However, Soybean isolate protein gel exhibits higher viscosity and
major starches. The more uniform and dense gel network results in

excessive moisture and low content of carbohydrates and proteins


viscosity, but the printing performance is not ideal due to the low

treatment and printing process. Printability assessment is a systematic


Cassava starch hydrogel is a relatively “weak” gel. It has a large

material. And its extrudability may be limited due to the higher

Wheat starch exhibits the best printing performance among the

Vegetables and fruits have undesirable printability due to their


viscosity in non-shearing processes, and the viscosity is greatly
Potato starch has modifiable viscosity and good transparency.

issue involving extrudability, dimensional accuracy, and stability of the


Soybean isolate protein gel has good water holding capacity.
Rice flour paste does not behave like an ideal shear thinning

Surimi is a sol with pseudoplasticity, i.e., it presents higher

rheological properties that are not suitable for 3D printing.


However, weak gel strength and too easy extrusion are its

3D printed objects (Kadival, Kour, Meena, & Mitra). Evaluation tech­


Printing characteristics of the material and the effect of physical treatment on it.

niques for physically treated substrates are common. Typically the


reduced during the extrusion and shearing process.

rheological properties of the pretreated samples are measured with a


rheometer to assess the extrudability of the substrate (Ma, Schutyser,
Boom, & Zhang, 2022). The yield stress of the substrate, which is related
to the printing extrusion force, is also measured as a parameter to assess
that give proper rheological properties
Material behavior and characteristics

limitations as a 3D printing material

extrudability. Moreover, the yield stress also reflects the mechanical


strength of the ink. This is because the ink needs to be strong enough to
support the subsequent build-up layers after deposition. Stability is also
significantly influenced by the printer parameters (Hussain, Malakar, &
Arora, 2022). Printing accuracy can usually be determined based on
good print quality.

two-dimensional and 3D structures. Depending on the type of substrate,


the effect of physical pretreatment on the printability is shown in
gel firmness

flow point

Table 1.

2.2. Effect of electrothermal regulated pretreatment on printing


performance
Soy protein
Rice flour
example

Cassava

spinach
powder
Surimi

isolate
Potato

Wheat

In 3D food printing pretreatment, heating substrates such as proteins


starch

starch

starch
Food

and starches can affect their structure and conformation, thereby


changing the printability together with the structural stability of the
vegetables

substrate. Electrothermal regulation is the most common choice for


Substrate

Fruit and

pretreating printing substrates. The effect of pre-printing electrothermal


Protein
Table 1

Starch

regulation on the printing characteristics and performance of the sub­


type

strates is shown in Fig. 1.

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Y. Lv et al. Trends in Food Science & Technology 133 (2023) 231–243

Fig. 1. The effect of pre-printing electrothermal regulation on the printing characteristics and performance (a) egg yolk paste, (b) dough.

The method of heating thermosensitive materials to prepare gels is samples have higher stack height and better structural integrity after
also widely used in the pretreatment of printing substrates. These ma­ baking, compared to the untreated samples (Pulatsu et al., 2021).
terials have a conformation of coils at higher temperatures, but as the Similar conclusions were reached in studies that were using the ther­
amount of heat decreases, they begin to form helical structures and momechanical process to treat wheat dough (Masbernat et al., 2021).
aggregates, thereby forming a gel (He, Zhang, & Fang, 2020). Liu et al.
dissolved carrageenan, xanthan gum, and starch in a certain ratio in
water, prior to heating the mixtures in a water bath at 90 ◦ C for 30 min. 2.3. The effect of energy-carrying electromagnetic field pretreatment on
They finally cooled the mixture to obtain a multi-component gel system printing results
that could be used for 3D printing (Zhenbin Liu, Bhandari, Prakash,
Mantihal, & Zhang, 2019). Egg yolk was heated in a water bath and then Compared with traditional heating methods, microwaves are
cooled, based on the heat-induced gelation property. This aids to expand increasingly being used in the food processing field because of their easy
the application of protein weak gelation system without additives in control and temperature management (Fu et al., 2012; Limei Wang
extrusion-based 3D food printing. The effect of temperature on the et al., 2020). In the pretreatment of food printing, microwave heating
printing characteristics and performance of egg yolk paste is shown in can enhance the textural and water retention properties of the gel (L. Ji,
Fig. 1a. Egg yolk paste that was heated at 72 ◦ C for 12 min was easily Xue, Zhang, Li, & Xue, 2017). The substrate composition can affect its
extruded but could not maintain the printed structure, while the yolk physical properties, therefore, researchers synergistically applied mi­
paste heated at 76 ◦ C for 8 min had good printability and structure crowave treatment and additives to gel inks to improve their printing
retention. Further increasing the heating temperature resulted in diffi­ characteristics. This resulted in significant improvements in printing
cult extrusion of the substrate (L. Xu, Zhang, & Bhandari, 2020). In accuracy. The synergistic effects of microwave and additives in
another study, gels made from cassava starch that had been dry heat pre-treatment on the printing effect are shown in Fig. 2, where Fig. 2a
treated at 130 ◦ C for 4 h also had good 3D printing characteristics and shows the synergistic effect of microwave and Ca2+ on the buckwheat
brought out products with higher resolution (Bianca C. Maniglia et al., starch-pectin gel system. The microwave treatment condition was
2020). Electrothermal treatment is widely used in the preparation of heating at 700w for 30 s. Compared with the control group, the treated
printing gels. Due to the different thermal properties of the substrates, printed products have a more desirable structural size and shape fidelity.
there is a considerable difference in pretreatment conditions for Fig. 2b shows the synergistic effects of microwave and salt on the soy­
starch-based and protein-based or other gels. Pre-experiments may be bean isolate protein-strawberry ink system. Different microwave power
required to determine the proper range of conditions in practical (30,50, and 70 W) were selected to pre-treat the printing ink for 4 min. It
applications. is clear that the product that contains salt ink that has been treated by
For smooth extrusion and good printing effects it is generally the maximum power microwave has the most desirable forming effect
required that the dough have proper rheological properties and struc­ (Fan, Zhang, Liu, & Ye, 2020; C. Guo, Zhang, & Devahastin, 2021b). A
ture retention in 3D food printing (Yang, Zhang, Prakash, & Liu, 2018). microwave was also used in the pretreatment of printing dough. Sun
Pulatsu et al. continuously heated the dough used for 3D printing by et al. pretreated wheat dough using a microwave, and this increased
hotplate at 55 ◦ C for 3 min. The results showed that pre-heating greatly both the surface viscosity and elasticity of the dough, while improving
improved the printability and shape stability of the dough system by the precision of printed products (Y. Sun, Zhang, & Chen, 2020). A
yielding dense networks and affecting conformational changes. The similar conclusion was reached by Villanueva et al. in the microwave
printing performance is shown in Fig. 1b, where the pre-heated dough pretreatment of the rice flour of gluten-free dough (Villanueva, Har­
asym, Munoz, & Ronda, 2019). Whether treated directly or in synergy

233
Y. Lv et al. Trends in Food Science & Technology 133 (2023) 231–243

Fig. 2. Synergistic effect of microwave and additives in pre-treatment on the printing results (a) microwave and Ca2+ , (b) microwave and salt.

with other techniques, microwave pretreatment achieved desirable re­ homogenization treatment to modify okara fiber. The resulting dough
sults in a short period of time. This provides researchers with an containing modified okara fiber exhibited excellent printing perfor­
essential approach to improve the printability of substrates unsuitable mance. The treated okara fiber exhibited better swelling as well as water
for 3D printing. and oil-holding capacities, both of which are extremely important for
In addition to the microwave, other energy-carrying electromagnetic printing inks. Adding modified okara fiber has also been seen to improve
fields have great potential for use in the pretreatment of printing sub­ the water-holding capacity and printing stability of the dough (Y. Liu
strate. Compared with microwaves, radio frequency electromagnetic et al., 2021). The shape accuracy of the 3D printed snacks was also
waves (RF) treatment exhibits better uniformity and controllability due improved by adding ultrasonic pre-treated bran to the dough, mainly as
to lower electromagnetic frequency (Xia, Gou, Zhang, Li, & Jiang, a result of the increased dough’s viscosity (Habus et al., 2021). The
2018). Radio frequency-treated potato starch gels displayed better combined treatment of energy-carrying electromagnetic fields can
printing performance than the untreated ones, thereby resulting in merge the advantages of different methods to obtain more desirable
products with higher stack height, line uniformity, and structural sta­ printing results. Xu et al. used ultrasonic-microwave combined pre­
bility. This is because the RF treatment rearranges the internal structure treatment in 3D printing pretreatment of the wheat starch-papaya sys­
of the potato starch granules, thereby causing a more stable and orga­ tem, and the results showed that in addition to the effect of microwave,
nized internal structure (S. Ma, Schutyser, et al., 2022). The electric ultrasound increased the ratio of bound water and shape retention
field-treated starch hydrogels displayed similar properties. Printed ability, which was beneficial in improving the printing characteristics of
products using pulsed electric field-treated wheat starch gels have the system (Xu, Zhang, & Bhandari, 2020).
smoother surfaces, higher hardness, and lower adhesiveness (Bianca
Chieregato Maniglia et al., 2021). 3. The research progress of physical regulation techniques in 3D
food printing process
2.4. The effect of ultrasonic pretreatment on printing results
During the 3D food printing, according to the path given by the
In the food processing industry, ultrasound, as an emerging tech­ control system, the substrates are discharged from the barrel to accu­
nique with promising applications, is often used in combination with mulate in the designated position and finally solidify into the shape. In
other methods (Singla & Sit, 2021). Combinations of ultrasound and this process, different forming modes has been seen to obtain different
other techniques are also used in 3D food printing pretreatment. Chen printing effects. Using physical regulation methods to change the phase
et al. investigated the effects of ultrasonic-assisted and water immersion change process and rheological properties of the printing substrates is
thawing on gelling as well as 3D printing properties of silver carp surimi. also one of the research directions in recent years, specifically involving
The results showed that a more desirable surimi quality and printing electrothermal regulation, microwave regulation, infrared regulation,
accuracy could be obtained by using ultrasonic-assisted thawing (H.-z. etc.
Chen, Zhang, & Rao, 2021). Liu et al. used ultrasonic and

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Y. Lv et al. Trends in Food Science & Technology 133 (2023) 231–243

3.1. The effect of forming mode on 3D printing results printing results than extrusion-based printing. Selective laser sintering is
mainly used for printing substrates with low melting points. It is able to
Forming modes were selected according to different printing mate­ print complex structures with different layers of material, thereby
rials to achieve different printing effects (Varvara, Szabo, & Vodnar, printing products with variable textures (Godoi, Prakash, & Bhandari,
2021). Among the various forming modes, the extrusion-based printing 2016; Zhenbin Liu, Zhang, Bhandari, & Wang, 2017). Binder jetting
is the most widely used and requires appropriate flow and viscoelasticity allows more dry matter content for fast printing without increasing the
of the substrate. They affect the shape fidelity and mechanical properties printing temperature. This helps to print complex geometries more
of the printed product (Liu, Zhang, & Bhandari, 2018). The method and easily (Holland, Foster, & Tuck, 2019). Inkjet printing uses a
parameters of the extrusion-based printing can also have a large impact low-viscosity substrate, typically used for food surface filling and
on the texture and forming effect of the printed product (Gue­ pattern decoration with a simple printing process (Pallottino et al.,
nard-Lampron, Masson, Leichtnam, & Blumenthal, 2021). Table 2 de­ 2016).
scribes the key printer parameters that affect the printability of 3D food The choice of printing mode and its parameters considerably de­
printing. Le Tohic et al. investigated the effect of the extrusion-based 3D termines the forming effect. Many researchers have paid attention to
printing on the properties of processed cheese. The result showed that hardware and parameter settings, but more research is needed to expand
cheese prepared by extrusion-based printing exhibits lower hardness the application of various printing modes in 3D food printing.
and higher degrees of meltability, compared to untreated cheese (Le
Tohic et al., 2018). Guo et al. compared the flow characteristics and 3.2. The effect of in-process electrothermal regulation on 3D printing
profile of syringe-based and screw-based printed products through results
computational simulations and printing experiments. The results
showed that different extrusion methods have a great impact on the The structural transformation of the starch gels is influenced by the
printing results. Screw-based 3D food printers may not be able to printing temperature. As the temperature increases, the gel network
extrude high-viscosity inks, while syringe-based 3D food printers is more becomes more complete, three-dimensional, and homogeneous, thereby
suitable for actual printing (C.-F. Guo, Zhang, & Bhandari, 2019). Liu improving the mechanical strength of the gel system (Zeng, Chen, Chen,
et al. also found that screw-based printing was not suitable for food & Zheng, 2021). Tian et al. changed the incubation temperature of the
substrates with high viscosity and mechanical strength in substrate by heating coils during printing, thereby resulting in
extrusion-based printing of starch gels. The printed products could not temperature-dependent hybrid gel inks with suitable rheological prop­
obtain the proper mechanical strength to support the subsequent erties for better forming results. The results showed that the gel inks
deposited layers, thereby resulting in compression distortion and low exhibited the best printing characteristics at 45 ◦ C (Tian et al., 2022). In
resolution (Z. B. Liu, Zhang, Bhandari, & Yang, 2018). the 3D printing process of potato starch gel, Liu et al. found that the
Most of the current 3D food printing research uses conventional suitable printing temperature makes the starch gel with smooth extru­
single-nozzle extrusion 3D printing, while dual extrusion printing and sion, good printing accuracy, and high structural strength (Zipeng Liu,
coaxial extrusion printing techniques are gaining attention from re­ Chen, Zheng, Xie, & Chen, 2020). Similar conclusions were reached in
searchers for their ability to achieve special printing effects. Liu et al. the printing of other materials such as oleogels (Kavimughil, Leena,
used dual extrusion printing to produce more complex geometries and Moses, & Anandharamakrishnan, 2022) and soy protein isolate pastes
more attractive multi-material structures by controlling material dis­ (J. Chen et al., 2022). The effect of the electrothermally regulated
tribution (Liu, Zhang, & Yang, 2018). Comparing coaxial extrusion printing temperature during printing on the printing structure and sta­
printing with normal extrusion based printing, the researchers found bility is shown in Fig. 3. Further, Fig. 3a shows the different forming
that coaxial extrusion printing can not only precisely control the gela­ effects of hybrid gelator inks at different printing temperatures (25, 45,
tion of the substrate by embedding functional materials (Vancau­ and 65 ◦ C). When the printing temperature was low, the ink line surface
wenberghe, Verboven, Lammertyn, & Nicolaï, 2018), but also accurately of the one-dimensional (1D) model was rough and showed obvious ag­
control the texture of the printed product (Chao et al., 2022). gregation, and the three-dimensional (3D) model showed obvious col­
Several other forming modes are capable of achieving different lapses. It indicates that the ink is too thin, resulting in poor printing

Table 2
Impact of 3D-printer parameters on printability.
Control 3D Printer/model Food example Effects on printability Reference
parameters

Nozzle Shiyin Co. Ltd. Composite mixtures of Significant differences in the printing accuracy and (Y. M. Shi, Zhang, &
diameter potato starch, whey shape retention between nozzle diameters Bhandari, 2021)
protein and oleogel
Nozzle Shiyin Technology Co., Ltd. Taro pastes Too fast or too slow speed all had negative effect on Huang, Zhang,
movement the accuracy of products. The appropriate printing Bhandari, and Liu
speed speed also takes into account the important factor of (2020)
printing time
Extrusion rate The 3D printing system composed of the following Lemon juice gel A suitable extrusion rate can achieve a smooth line (F. L. Yang, Zhang,
three major parts: (i) feed hopper with auger mixer with uniform diameter. And a reasonable Bhandari, & Liu, 2018)
and conveyor, (ii) an extrusion system, (iii) and a X- combination of extrusion rate, nozzle movement
Y-Z positioning system speed, and nozzle diameter helps improve printing
performance and increase printing efficiency
Printing path 3D printer mod. Delta 2040 (Wasp project, Italy) Batters The defined infill pathway resulted in the distribution Derossi, Caporizzi,
equipped with a screw-extrusion system of pores in 3D printed products Oral, and Severini
(2020)
Height ratio Z Morph multi tool 3D printer with thick paste Rice flour and jaggery It is found to be negatively correlated to layer height. (S. Thangalakshmi,
extruder paste The optimal height ratio and layer height contribute Kumar, & V, 2022)
to a uniform print layer and good structure retention
Post cooling An extrusion-based 3D printer, PORIMY Co. Ltd. Baking dough Fast-cooling is used to enhance the shape retention of (F. Yang, Zhang, Fang,
printed samples. The dough should be fast-cooled to & Liu, 2019)
65 ◦ C for more than 10 min to have desired shapes
and structures

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Y. Lv et al. Trends in Food Science & Technology 133 (2023) 231–243

Fig. 3. The effect of the electrothermally regulated temperature during printing on the printing structure and stability (a) One-dimensional and three-dimensional
printing structure of hybrid gelator inks at different printing temperatures, (b) the forming effect and stability of the soy protein isolate pastes with additives at
different temperatures.

performance. When the printing temperature was too high, the ink lines denatured in a short time under conventional heating methods. The
in the 1D model were interrupted in many places, and the 3D model protein has a high dielectric constant, microwave treatment can change
structure had obvious broken wires that have fallen. This is due to the its activity and structure in a relatively short period of time with the aim
fact that the ink cannot be extruded smoothly from the nozzle, while at of promoting the gelation of the protein substrates (H. Jiang, Liu, &
close to 45 ◦ C, the printed structure had good shape retention (Tian Wang, 2018; Jiao et al., 2019; X. Liu, Feng, et al., 2018). Therefore,
et al., 2022). Fig. 3b shows the forming effect and stability of the soy microwave regulation is ideal for the protein substrates in the printing
protein isolate pastes with the addition of sodium alginate and gelatin at process to achieve uniform heating. Related articles reported that
different temperatures (25, 35, 45 ◦ C). The higher printing temperature transglutaminase,(TGase) can effectively improve the strength and
resulted in better structural retention of the mixed pastes, especially hardness of surimi gels (Dong et al., 2020), Zhao et al. investigated the
when the printed object height exceeded 10 mm (Fig. 3b, printing2), and synergistic effect of microwaves and TGase on the phase transition
no collapse was observed after storage for 6 h. This indicates that the process of surimi from fluid to gelatinized solid. The mechanism of
coordinated effect of electrothermal temperature regulation and opti­ self-gelation of surimi during the 3D printing process was clarified, as
mized printing substrate formulation can bring out 3D printing struc­ shown in Fig. 4. The result showed that microwave heating of surimi
tures with high precision and stability (J. Chen et al., 2022). increased TGase activity. This contributed to the surimi transform
In the 3D food printing process, using electrothermal modulation gradually into a solid gel during the deposition process. When the mi­
also enables the pre- and post-processing to be integrated into the food crowave power was in the range of 40–50 W/g, the extrusion nozzle
printer, thereby reducing printing pre- and post-treatment time. Diañez temperature was close to 40 ◦ C, thereby resulting in an increase in TGase
et al. heated the extrusion nozzle during the printing process to promote activity to promote the conversion of surimi into a solid gel. This was
the in-situ temperature-induced gelification. Compared to preparing the conducive to improving the shape stability of the printed products. The
gel in the substrate pretreatment, this method minimizes the processing printed hollow spheres in Fig. 4 exhibit good shape retention (Zhao
time without additional additives (Diañez et al., 2019). Khodeir et al. et al., 2021). To investigate whether the above method is applicable to
developed a printing nozzle using ohmic heating to pre-bake a cake different gelation mechanisms of different proteins, this team also
batter in continuous flow. This reduced baking time in post-treatment of applied the synergistic effect of microwaves and TGase on soy proteins.
3D printed products (Khodeir et al., 2021). This resulted in self-gelation of soy proteins that are not suitable for
direct 3D printing (X. Yu, Wang, et al., 2022). The synergistic effect of
microwaves and TGase provides a new method to improve the quality of
3.3. The effect of in-process microwave regulation on 3D printing results
protein-based 3D printing. However, when the microwave power is
high, the protein is rapidly cross-linked leading to the printing substrates
In the printing process, the protein substrates cannot be fully

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Y. Lv et al. Trends in Food Science & Technology 133 (2023) 231–243

Fig. 4. Synergistic effect of microwave and Tgase on the self-gelation of surimi during the 3D printing.

cannot be smoothly extruded. This is an issue that needs to be resolved effects to improve structural stability during printing, the on-board
when microwaving protein-based material during printing to improve integration of thermal stabilization solutions can also be used to inte­
their printability. grate possible post-treatment into the printing system. Using infrared
heating to integrate post-printing cooking into a food printer, Hertafeld
3.4. The effect of in-process infrared regulation on 3D printing results et al. have shown the ability to simultaneously extrude and cook
printing substrates with extreme precision. Due to the high resolution of
Typically near-infrared (NIR) 3D printing uses NIR lasers to facilitate infrared spot, it is possible to precisely heat the different printing sub­
the cross-linking and curing of NIR-sensitive inks to form 3D structures strates in the printed product to give rise to new flavor profiles (Herta­
(Zhu, Zhang, Yang, Liu, & Liu, 2020), while in the 3D food printing NIR feld et al., 2019). The integrated IR regulation during printing can
is mainly applied for its thermal effect. Fahmy et al. used starch-based reduce post-processing time. This is a potential way to increase pro­
food as the printing substrate and heated each layer of the 3D struc­ ductivity for printed products with post-processing needs.
ture automatically during printing with the help of a NIR spot heater,
thereby improving the thermal stabilization of printing substrate by
temperature increment and moisture loss. This printing method can
reduce the diffusion and mechanical deformation of the substrate after
extrusion to form a more stable printed structure and prevent structural
collapse (Fahmy et al., 2021). In addition to being able to use thermal

Fig. 5. The effect of post-printing physical regulation on the degree of printed product deformation (a) different mechanisms of deformation caused by microwave
drying, infrared drying and hot air drying, (b) microwave-induced deformation of samples with different salt contents.

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4. The research progress of post-printing physical regulation post-treatment directionally changes the printed samples with 2D
techniques in 3D food printing structure into 3D structure. This microwave-induced spontaneous
deformation provides a basis for 4D food printing (He, Zhang, & Deva­
4.1. Physical regulation of 3D printing shaping effect hastin, 2020). Shi et al. used purple potato puree and oleogel for
dual-nozzle printing, and utilized microwave as an external thermal
Microwave treatment is an essential factor to regulate the shaping stimulus to treat the printed products. Due to the differences in dielectric
effect after 3D printing. Microwaves can induce spontaneous shape properties and flow characteristics, different materials melt at different
changes of 3D printed products. This mainly relies on the rapid dehy­ degrees and rates during the microwave treatment. This provides the
dration of materials. The differences of microwave penetration depth driving force for achieving spontaneous shape change (Y. Shi, Zhang, &
and material dielectric properties lead to uneven shrinkage deformation, Phuhongsung, 2022). Exploiting the difference in material properties
which provides an effective method for 3D food printing to achieve provides a new idea for achieving spontaneous deformation in 4D food
controlled deformation (Teng, Zhang, & Mujumdar, 2021). The effect of printing. A similar control strategy was used in the controlled defor­
post-printing physical regulation on the deformation degree is shown in mation of the double layer structure of 3D printed pumpkin/paper by
Fig. 5. Fig. 5a shows mechanisms of deformation caused by different Fengying Chen et al. The different temperature response characteristics
drying methods. In the microwave drying process, the temperature of of the materials were exploited to create deformation drivers for
the inner layer of the printed product is higher than the outer layer, pumpkin puree dehydration shrinkage (F. Chen, Zhang, Liu, & Bhandari,
thereby causing the moisture migration from the inner layer to the 2021).
outside, which caused the inner layer first shrinkage. However, the One of the advantages of 4D printing is the ability to achieve sus­
shrinkage of internal layer cannot bend the printed products uniformly pended structures that 3D printing cannot do (Teng et al., 2021). The
due to the constraint and the final evaporation of moisture from the special printed structures that can be achieved by microwave treatment
outer layer. Compared with hot air drying and infrared drying, there was in 4D printing are shown in Fig. 6. In Fig. 6a, the substrate printed
no “accumulation bending effect” during microwave drying, the sample directly by 3D printing lacks support during the deposition process,
has a minimum of shrinkage and bending degree (Z. Liu et al., 2021a). thereby causing the collapse of the structure. This makes it impossible to
Fig. 5b shows the difference in microwave-induced deformation of form a suspended structure of petals, while 4D printing can first print a
samples with different salt content. As the salt content increases, the 3D structure with stable support, and then deform the product through
final bending angle after microwave treatment decreases significantly. microwave heating to form a stable suspended structure. In Fig. 6b, a
This is due to the fact that edible salt increases the dielectric properties long time hot air heating cannot make a single petal flower samples
of the printing substrate, which accelerate the heating and drying rate of deformation, but through microwave heating in a short period of time to
the printed product in the microwave treatment. The result is a more achieve a significant “flowering” effect due to the uneven heating
extensive hardening of the printed product surface, which helps to stop characteristics of microwaves, which leads to the connection between
the deformation (He, Zhang, & Devahastin, 2021). This method com­ the petal and stamen swelling to produce deformation drive. However,
bines the microwave treatment and the optimized substrate composition the hot air heating evenly heats the sample surface without producing a
to effectively control the amount of deformation. “flowering” drive (C. Guo, Zhang, Bhandari, & Devahastin, 2022b).
4D food printing is a targeted extension of 3D printing. It uses the Texture is one of the indicators for evaluating printed products.
stimulation of external factors to change the printing performance of the Physical regulation in the post-processing can effectively improve the
printed products (Jaspin, Anbarasan, Dharini, & Mahendran, 2021; Z. texture of the product. When C. Severini et al. baked the printed dough,
Liu et al., 2021b). Controlled deformation of 3D printed products is one they found that the moderate temperature avoided low expansion and
of the main forms of 4D food printing that can enhance the consumer surface crusting. This resulted in a decrease in the firmness of the
experience. He et al. used purple sweet potato purees for printing, fol­ confection (C. Severini, Azzollini, Albenzio, & Derossi, 2018). The
lowed by rapid dehydration of the samples using microwaves to induce texture of meat is affected by the method of reheating. For 3D printed
spontaneous shape change. The result showed that the bending angle of meat products with easy swallowing requirements, microwave heating
printed samples is related to their dehydration rate, which expectedly are able to avoid crusting from convection heating, thereby causing a
increases with increased microwave power. The microwave soft texture of the sample (Dick, Bhandari, & Prakash, 2021). Microwave

Fig. 6. Special structural changes achieved by microwave heating in 4D printing (a) comparison of deformation effects of 3D printing and indirect 4D printing, (b)
comparison of deformation effects induced by hot air and microwave heating.

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heating of materials has a significant puffing effect (Pompe, Briesen, & change is positively correlated with the microcapsule level and heating
Datta, 2020), which can be applied to post-processing of 3D printing. time (Tang, Zhang, et al., 2022). Guo et al. also combined color and
This helps to puff up 3D printed starch-based products into low-fat flavor changing microcapsules with the strategy of directional defor­
products similar to fried foods (Teng, Zhang, & Mujumdar, 2022). mation to realize the synchronous 4D printing process of deformation,
color changing, and aroma changing within 15 s, induced by a house­
4.2. Physical regulation of printed product color hold microwave oven. This provides a new research method for 4D
printing (C. Guo, Zhang, Bhandari, & Devahastin, 2022a).
Attractive colors can enhance the visual performance of food and To achieve color change in 3D food printing post-processing, Chen
become a driving force for purchase. The ideal color given to the et al. also used microwave heating for changing the acid-base environ­
printing products in 3D food printing post-processing is beneficial to ment of the printed product, thereby causing spontaneous color change
improve their sensory experiences. Using physical regulation methods to of pH-sensitive component in the sample. This method achieves post-
achieve spontaneous color change of 3D printed products is also one of printing physical regulation of the product color. As shown in Fig. 7c,
the directions of 4D food printing (Ghazal, Zhang, & Liu, 2019; Navaf the top and bottom are the samples before and after treatment, respec­
et al., 2022). The methods and samples of spontaneous color change tively. Microwave heating caused spontaneous color change of curcu­
using physical regulation techniques in post-treatment are shown in min, which is sensitive to pH, in the samples. The pictures show that the
Fig. 7. sample color changed completely after 1 or 2 min of microwave treat­
Microcapsule, one of the important new complementary technolo­ ment. The yellow dry lines that impair the appearance of the samples
gies in food research, is often used to embed functional ingredients to appeared on the surface after 3 min, thus it can be found that this
improve their storage stability (Bekhit, Sánchez-González, Ben Mes­ method has a high efficiency of color change (C. Chen, Zhang, Guo, &
saoud, & Desobry, 2016; Coelho et al., 2022). Energy-carrying electro­ Chen, 2021).
magnetic fields can be used as a source of stimulation to facilitate the Not all color changes are expected in the post-processing of 3D food
release of pigments from microcapsules to achieve color change (L. printed products. Many printed products are cooked and processed after
Tang, Liu, et al., 2022). In 3D food printing post-processing, microcap­ printing, usually by baking, microwaving, etc (Keerthana, Anukiruthika,
sule is used in combination with physical regulation techniques to Moses, & Anandharamakrishnan, 2020; Pulatsu et al., 2022). Discolor­
improve the color and flavor of printed products. Guo et al. added ation due to inappropriate heat treatment may reduce the sensory
different contents of microcapsules to buckwheat dough containing acceptability of 3D printed products. When S. Thangalakshmi et al.
yellow flesh peach. The pepper red pigment in the capsules was released analyzed the effect of steaming on the color of printed samples, they
during microwave heating due to the destruction of capsule wall found that different processing times may have different effects on the
composing of gelatin and gum Arabic. The experimental results showed color due to the composition of the substrate (S. Thangalakshmi, Arora,
that the color changes of the dough with different levels of microcap­ et al., 2022). Krishnaraj et al. compared microwave reheating with hot
sules exhibited different trends. The color change of printed products air drying and frying. The results showed the microwave-treated sam­
using microwaves stimulated microcapsules is shown in Fig. 7a (C. Guo, ples were the most similar in appearance to the untreated samples and
Zhang, & Devahastin, 2021a). Tang et al. have reached similar conclu­ preserved the color most completely (Krishnaraj, Anukiruthika,
sions. They used microwave-infrared heating as a stimulation source to Choudhary, Moses, & Anandharamakrishnan, 2019). For substrates such
achieve color and flavor changes in a white radish/potato gel product. as rice starch, which are more suitable for frying treatment (Theagar­
The color change effect is shown in Fig. 7b. Further, the degree of color ajan, Nimbkar, Moses, & Anandharamakrishnan, 2021), microwave,

Fig. 7. The methods and samples of spontaneous color change using physical regulation in post-treatment (a) the spontaneous color change of printed products using
microwave stimulated microcapsules, (b) microwave-infrared heating stimulates microcapsules to change the color, (c) microwave heating changes the pH to change
the color of product ingredients. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

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ultrasonic, and electric fields combined with frying can improve the Data availability
frying quality of printed products. They are beneficial in reducing color
variation and making the color more natural (Devi, Zhang, & Mujumdar, No data was used for the research described in the article.
2021; D. Yang et al., 2021).
Acknowledgements

4.3. Physical regulation of moisture in 3D printed products We acknowledge the financial support provided to us by the National
Key R&D Program of China (2022YFF1101200), which has enabled us to
Dehydration is a common post-processing method in food process­ carry out this study. The authors would like to express their gratitude to
ing. It can effectively improve storage conditions and extend shelf life. EditSprings (https://www.editsprings.cn) for the expert linguistic ser­
Timely regulation of moisture in 3D food printing post-processing can vices provided.
improve stabilization of printed products (Demei, Zhang, Phuhongsung,
& Mujumdar, 2022; Yoha, Anukiruthika, Anila, Moses, & Anandhar­ References
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