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Culture and Culture Media Lab
Culture and Culture Media Lab
- These media allow the visualization of metabolic differences - Indicator: Neutral red - Differentiate hemolytic pattern
between groups of bacteria of streptococci
- Example: MAC, BAP, eosin methylene blue (EMB) and Hektoen
Enteric Agar (HEA)
- These media are incorporated with antibiotics, dyes or Other selective media:
chemicals to inhibit the growth of other organisms.
- Gentamicin blood agar: Streptococcus
- Example: HEA, MAC, Xylose lysine desoxycholate (XLD) agar,
- Bacitracin chocolate agar: Haemophilus
Bismuth sulfate agar (BSA), Mannitol Salt Agar (MSA) and
- Blood agar plate with ampicillin: Aeromonas
Thayer-Martin Agar (TMA)
- Phenylethyl alcohol: Gram positive bacteria
- Colistin-Nalidixic acid (CNA) agar: Gram positive bacteria
D. ACCORDING TO USE
D. ACCORDING TO USE
5. Selective Media
5. Selective Media MEDIA DESCRIPTION
Inhibitory substances Hektoen Enteric Agar (HEA) Bile salt and dyes: Inhibit indigenous microbiota of LGIT;
used for recovery of fecal bacteria
pH indicator: Bromthymol blue
Gram Positive Bacteria Crystal/Gentian violet, MacConkey Agar (MAC) Bile salts and crystal violet: inhibit gram-positive bacteria;
used for recovery of fecal bacteria
basic/carbol fuchsin and bile salt
Xylose Lysine Desoxycholate Agar (XLD) Xylose, lysine, sucrose, 0.25% sodium desoxycholate
Gram Negative Bacteria Potassium tellurite and sodium and sodium thiosulfate; for fecal bacteria
azide Differentiate: Shigella and Salmonella
For Swarming Bacteria Alcohol and chloral hydrate Mannitol Salt Agar (MSA) Support growth of Staphylococcus aureus