ALKALINE PHOSPHATASE (ALP)

Continuous-spectrophotometric
DEA BUFFER
The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument: BS-480
Reagent preparation
Reagent 1: Use the Reagent A.
Reagent 2: Use the Reagent B.

Basics
Test information Reagent Volume Sample volume
No * R1 200 Standard 5
Test information Alp DEA R2 50 Increase
Full name Alp DEA R3 0 Decreased
St.No 2 R4 0

Reaction parámetres Result Setup

Reac Type Kinetic Direction Increase Decimal 0 Slope 1
Pri.Wave 412 Blank time Unit UI/L Inter 0
Sec.Wave 700 Reac.Time 57-76

Judgment Criteria
Absorbance 0 - 30000 Lin.Range 8-900 Prozone
Incre.Test Lin.Limit Q1 Q2 Q3 Q4
Decre.Test Subs.Limit PC Abs

Reference
Gender Sample Type Low limit High Limit
Male Serum 0 270
Female Serum 0 240

Calibration
Calibration Calibrators
Rule Two point linear Calibrators Lot.On Std Conc Expire Position
Replicate 3 Dest. Water 0
K S1 (*)
Judgement Criteria
Sensitivity
Factor Diff
SD
(*) entered
Blank Abs by user
Error Limit
Corr. Coeff.

Vers.03/21