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Tube Collection Guide
Tube Collection Guide
SERUM
GOLD TOP Thixothropic gel Number of Inversions: 5
+
Silica particles
SERUM CT: 30 MINUTES
Separator gel – inert material that undergoes a temporary change in viscosity during the centrifugation process
ANTICOAGULANTS
LAVENDER TOP Ethylenediamine Number of Inversions: 8
tetraacetic acid MOA: chelates calcium
Optimal anticoagulant conc: 1.5-2.2 mg/mL of blood
WHOLE
✓ Most commonly used anticoagulant for hemotological
BLOOD/PLASMA
testing
Important considerations:
• K2EDTA (most preferred), K3EDTA, LiEDTA, or
Na2EDTA can be used but NEVER USE Na3EDTA
Routine Hematological
studies - CBC, Reticulocyte
Insufficient EDTA Excessive EDTA
Count, modified Westergren
(overfilled tube) (underfilled tube)
of ESR
presence of clots or falsely (↓) HCT and ESR,
microclots degenerative changes in
WBC, false (↑) MCHC and
platelet count