Sheikh Khalifa Specialty Hospital - Ras Al Khaimah

Pathology Laboratory
Cytopathology Department Flowchart

Pre examination process Examination process Post examination process

Arrange all speciment in serial No for NGY LBC No

Specimen arrive order and Perform sample
grossing. Non Gyn Centrifuge sample No for GYN
LBC and wash with 2022
Cytolyst Perform QC Cytology
review of slides
QC Log

Mention sample description on

Package open test request form
Preparation of LBC
Gyn slides using Thinprep
2000 or 5000 yes

Print out slides and type sample Review slides by the pathologist
NO Specimen description and cytology orders
Raise Test in
evaluation for in HIS and LDM
acceptability.
question (TIQ)
and resolve. yes 2022
Additional order Additonal
check order file
YES Gynecological
Gyn
Perform Special Stain or IHC and
sample or Non
Gynecological Special stain or Yes submit slides to pathologist for
Specimen Receive in sample IHC request by review
2022 HIS and LDM and No Pathologist
TIQ LOG Lab connect
BOOK accessioned Non Gyn 10 mins fixation in
95% alcohol
Yes
Cell block
Cell block reqeusted NO

No
Mix with 5% agar solution PAP staining Report release
Non Gyn
LBC
LBC or
Convetnional
smear

Centrifuge and discard alcohol

Conventional smear

Perform Cytospin or smear

cytology
95% alcohol fixation for cell
block sample No for NGY Conventional

ILPA_901_4 Version 1.0 Page 1 of 1 Effective Date: 26-Nov-2017

 Sheikh Khalifa Specialty Hospital - Ras Al Khaimah
Pathology Laboratory
Histopathology Department Flowchart

Pre examination process Examination process Post examination process

Specimen arrive
Print the biopsy cassettes. After processing, verify number Fail
of cassettes and record in
tracking log 2022
Histopathology
QC file
Package open Arrange all specimen and Perform QC
cassetts in serial order. review of slides
2022 Perform embedding
TIQ LOG
BOOK

No Specimen
evaluation for
Perform the grossing
Raise Test in Trimm tissue till exposure of Pass
acceptability. tissue.
question (TIQ)
and resolve.
Tissue need
Yes decalcification
Yes Print the slide
Review slides by the pathologist
2022
Specimen Receive No Additional
in HIS and order file
accessioned Representative tissue kept in No Deeper or Recut
labelled cassettes. requested by
Yes pathologist
No
Match the slide No
Tissue kept for label with tissue Special stain,
decalcification embedded block IHC, request by
Pathologist
MG requested Yes
Verfiy tissue is
No by pathologist
proper fixed in
formalin
Yes

Yes Yes
Check the tissue Yes
No for decalcified

Sectioning of the tissue on

Fill cassette tracking 3 to 5 micron
Yes
form.

Kept tissue for more

decalcification Tissue cassettes kept for Kept slides for drying
more fixation
Perform Special Stain or IHC and
submit slides to pathologist for
review
Put rack in tissue processor
Select overnight programme

Perform H & E staining and

coverslipping

Next day check the

tissue again for
fixation

Start tissue processor Report release in the

Put the gross tamplate in the HIS HIS
Yes

Submit slides to the pathologist

ILPA_901_4 Version 1.0 Page 1 of 1 Effective Date: 26-Nov-2017

 Sheikh Khalifa Specialty Hospital - Ras Al Khaimah
Pathology Laboratory
Histopathology Department Flowchart

Pre examination process Examination process Post examination process

Specimen arrive
Print the biopsy cassettes. After processing, verify number Fail
of cassettes and record in
tracking log 2022
Histopathology
QC file
Package open Arrange all specimen and Perform QC
cassetts in serial order. review of slides
2022 Perform embedding
TIQ LOG
BOOK

Specimen Perform the grossing

No evaluation for Trimm tissue till exposure of Pass
Raise Test in acceptability.
question (TIQ) tissue.
and resolve.
Tissue need
decalcification
Yes Yes Print the slide
Review slides by the pathologist
2022
Specimen Receive No Additional
in LDM and order file
accessioned Representative tissue kept in Deeper or Recut
labelled cassettes. requested by
Yes pathologist
No
Match the slide No No
label with tissue Special stain,
Tissue kept for embedded block IHC, request by
Pathologist Yes
decalcification MG requested
Verfiy tissue is
by pathologist
proper fixed in
No No
formalin
Yes

Yes Yes
Check the tissue
No for decalcified

Yes Sectioning of the tissue on

Fill cassette tracking 3 to 5 micron
form.

Kept tissue for more

decalcification Tissue cassettes kept for Kept slides for drying
more fixation
Perform Special Stain or IHC and
submit slides to pathologist for
review
Put rack in tissue processor
Select overnight programme

Perform H & E staining and

coverslipping

Next day check the

tissue again for
fixation

Start tissue processor Report release in the

Put the gross tamplate in the HIS LDM
Yes

Submit slides to the pathologist

ILPA_901_4 Version 1.0 Page 1 of 1 Effective Date: 26-Nov-2017

 Sheikh Khalifa Specialty Hospital - Ras Al Khaimah
Pathology Laboratory
Histopathology Department Flowchart

Pre examination process Examination process Post examination process

Specimen arrive
Print the biopsy cassettes. After processing, verify number Fail
of cassettes and record in
tracking log 2022
Histopathology
QC file
Package open Arrange all specimen and Perform QC
cassetts in serial order. review of slides
2022 Perform embedding
TIQ LOG
BOOK

NO Specimen Perform the grossing

evaluation for Trimm tissue till exposure of Pass
Raise Test in acceptability.
question (TIQ) tissue.
and resolve.
Tissue need
decalcification
Yes Yes Print the slide
Review slides by the pathologist
2022
Specimen batch No Additional
Receive in lab order file
connect Representative tissue kept in Deeper or Recut
labelled cassettes. requested by
Yes pathologist

Match the slide No No

Specimen Recived in label with tissue Special stain,
LDM and accessioned embedded block IHC, request by
Pathologist Yes
MG requested
Verfiy tissue is
by pathologist
proper fixed in
No
formalin
Tissue kept for
decalcification Yes
Yes Yes

Sectioning of the tissue on

Fill cassette tracking 3 to 5 micron
Check the tissue form.
for decalcified
No

Yes

Tissue cassettes kept for Kept slides for drying

more fixation
Perform Special Stain or IHC and
submit slides to pathologist for
Kept tissue for more review
Put rack in tissue processor
decalcification Select overnight programme

Perform H & E staining and

coverslipping

Next day check the

tissue again for
fixation

Start tissue processor Report release in the

Put the gross tamplate in the HIS HIS
Yes

Submit slides to the pathologist

ILPA_901_4 Version 1.0 Page 1 of 1 Effective Date: 26-Nov-2017