HIGH-HEAT DECONTAMINATION CO2 INCUBATOR

110 - 120 Voltage

Installation and
Operation Manual
SCO6AD
Previously designated:
3552
SCO6AD
HIGH-HEAT DECONAMINATION CO2 INCUBATOR
Installation and Operation Manual

Part Number (Manual): 4861712

Revision: December 16, 2014

These units are TÜV CUE listed as air jacketed CO2 Incubators for professional, industrial, or
educational use where the preparation or testing of materials is done at approximately atmospheric
pressure and no flammable, volatile, or combustible materials are being heated.

These units have been tested to the following requirements:

CAN/CSA C22.2 No. 61010-1:2012

CAN/CSA C22.2 No. 61010-2-010 + R:2009
UL 61010A-2-010:2002
UL 61010-1:2012
EN 61010-1:2010
EN 61010-2-010:2003

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TABLE OF CONTENTS
INTRODUCTION........................................................................................................................................... 5
General Safety Considerations ................................................................................................................. 5
Engineering Improvements ....................................................................................................................... 6
Contacting Assistance ............................................................................................................................... 6
RECEIVING YOUR INCUBATOR ................................................................................................................ 7
Inspecting the Shipment ............................................................................................................................ 7
Inspection and Orientation ........................................................................................................................ 8
Recording Data Plate Information ............................................................................................................. 9
INSTALLATION .......................................................................................................................................... 12
Ambient Conditions ................................................................................................................................. 12
Power Source .......................................................................................................................................... 12
Environmental Factors ............................................................................................................................ 12
Lifting ....................................................................................................................................................... 13
Leveling ................................................................................................................................................... 13
Connect the CO2 Supply to the Incubator ............................................................................................... 14
Installation Cleaning and Disinfection ..................................................................................................... 15
Deionized and Distilled Water ................................................................................................................. 15
Shelving Installation ................................................................................................................................ 15
CONTROL PANEL OVERVIEW ................................................................................................................. 16

OPERATION ............................................................................................................................................... 19
Theory of Operation ................................................................................................................................ 19
Preparing the Incubator for Use .............................................................................................................. 22
Humidifying the Incubator ....................................................................................................................... 23
Set the Temperature Set Point ................................................................................................................ 24
Calibrate the Temperature display .......................................................................................................... 25
Set the Over Temperature Limit .............................................................................................................. 27
Muting the Audible Temperature Alarm .................................................................................................. 28
Set the CO2 Set Point.............................................................................................................................. 29
Calibrate the CO2 Display ........................................................................................................................ 30
Muting the Audible CO2 Alarm ................................................................................................................ 32
No Gas Supply Alarm (NGS) .................................................................................................................. 32
Decontamination Cycle ........................................................................................................................... 33
Aborting the Decontamination Auto Cycle .............................................................................................. 36
Condensation and the Dew Point ............................................................................................................ 37
Loading the Incubator ............................................................................................................................. 38
Accessory Compatibility .......................................................................................................................... 38
Data Output Capabilities ......................................................................................................................... 38
USER MAINTENANCE ............................................................................................................................... 39
Cleaning and Disinfecting ....................................................................................................................... 39
Minimizing Contamination Exposure ....................................................................................................... 40
HEPA Filter and Gas Lines ..................................................................................................................... 41
Storage of the SCO6AD Incubator .......................................................................................................... 41
Door and Electrical Components ............................................................................................................ 41
UNIT SPECIFICATIONS............................................................................................................................. 42
Weight ..................................................................................................................................................... 42
Dimensions .............................................................................................................................................. 42
Capacity .................................................................................................................................................. 42
CO2 .......................................................................................................................................................... 43

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 Temperature ............................................................................................................................................ 43
Power ...................................................................................................................................................... 43
Conversion Table .................................................................................................................................... 43
PARTS LIST ............................................................................................................................................... 44
Components ............................................................................................................................................ 44
Ordering Parts and Consumables ........................................................................................................... 45
ACCESORIES ............................................................................................................................................ 46

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INTRODUCTION

Thank you for purchasing a Sheldon SCO6AD High-Heat Decontamination CO2 incubator. We
know that in today’s competitive market place, customers have many choices when it comes to
constant temperature equipment. We appreciate you choosing ours. Our continued reputation as a
leading laboratory product manufacturer rests with you. Sheldon Manufacturing, Inc. stands behind
our products and want to let you know we are here if you need us.

These incubators are intended for laboratory, industrial, and educational microbiological
applications. These incubators are not intended for use in hazardous or household locations.

Before you use the SCO6AD read this entire manual carefully to understand how to install, operate,
and maintain the incubator in a safe manner. Keep this manual available for use by all operators.
Ensure that all operators are given appropriate training before the incubator begins service.

GENERAL SAFETY CONSIDERATIONS

Note: Failure to follow the guidelines and instructions in this manual may create a protection
impairment by disabling or interfering with the unit’s safety features. This can result in injury
or death.

Your SCO6AD and its recommended accessories are designed and tested to meet strict safety
requirements. It is designed to connect to a power source using the specific power cord type
shipped with the unit.

For continued safe operation of your incubator, always follow basic safety precautions including:

 Always plug the incubator power cord into an earth grounded electrical outlet that conforms
to national and local electrical codes. If the incubator is not grounded properly, parts such
as knobs and controls can conduct electricity and cause serious injury.

 Avoid damaging the power cord. Do not bend it excessively, step on it, or place heavy
objects on it. A damaged cord can be a shock or fire hazard. Never use a power cord if it is
damaged.

 Always position the unit so that the user can quickly unplug it in the event of an emergency.

 Do not attempt to move the unit while in operation or before the unit has cooled.

 Do not stack SCO6ADs without use of a stacking rack.

 Use only approved accessories. Do not modify system components. Any alterations or
modifications to your incubator can be dangerous and void your warranty.

 Follow all local ordinances in your area regarding the use of this incubator. If you have any
questions about local requirements, please contact the appropriate agencies.

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INTRODUCTION (CONTINUED)

ENGINEERING IMPROVEMENTS
Sheldon Manufacturing continually improves all of its products. As a result, engineering changes
and improvements are made from time to time. Therefore, some changes, modifications, and
improvements may not be covered in this manual. If your unit’s operating characteristics or
appearance differs from those described in this manual, please contact your Shel Lab dealer or
distributor for assistance.

CONTACTING ASSISTANCE
If you are unable to resolve a technical issue with the incubator, please contact Sheldon Technical
Support. Phone hours for Sheldon Technical Support are 6am – 4:30pm Pacific Coast Time (west
coast of the United States, UTC -8). Please have the following information ready when calling or
emailing Technical Support: the model number and the serial number (see page 9).

EMAIL: tech@shellab.com PHONE: 1-800-322-4897 extension 4, or (503) 640-3000 FAX: (503)

640-1366

Sheldon Manufacturing INC.

P.O. Box 627
Cornelius, OR 97113

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RECEIVING YOUR INCUBATOR

Before leaving the factory, SCO6ADs are packaged in high-quality shipping materials to provide
protection from transportation-related damage.

When an incubator leaves our factory, safe delivery becomes the responsibility of the carrier.
Damage sustained during transit is not covered by the manufacturing defect warranty. When you
receive your SCO6ADs inspect it for concealed loss or damage to the interior and exterior. If
you find damage, follow the carrier’s procedure for claiming damage or loss.

INSPECTING THE SHIPMENT

Carefully inspect the shipping carton for damage. Report any damage to the carrier service that
delivered the incubator. If the carton is not damaged, open the carton and remove the contents.
Carefully check all packaging before discarding. Save the shipping carton until you are certain that
the unit and its accessories function properly.

The unit should come with an Installation and Operation Manual, warranty card and a Certificate of
Compliance. Verify that the correct number of shelves, shelf slides, leveling feet, and other
components have been included (see the table below for quantities).

Note: The SCO6AD requires a CO2 gas regulator. A regulator is not included with the SCO6AD.
Please see the Accessories section on page 46 if you wish to order one from Shel Lab.

Included Accessories
1 Anti-Microbial 4 Leveling Feet 1 Power Cord 1 Stainless Steel 3 Stainless Steel
Copper Token Humidity Pan Shelves

6 Stainless Steel 4 Stainless Steel 1 Tubing Kit

Shelf Slides Shelf Standards

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RECEIVING (CONTINUED)

INSPECTION AND ORIENTATION

Side Access Port
with Copper Filter

OTL and Data Main Control Panel

Sample Panel
CO2 Panel Temperature Decontamination Incubation
Panel Cycle Panel Chamber

Interior
Exterior Door
Glass
Gasket
Viewing
Door

Shelf
Standard

Exterior Door
Latch
Exterior
Inner Door Door
Latch

Inner Door
Gasket

Humidity Pan
with Copper
Token

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RECEIVING (CONTINUED)

RECORDING DATA PLATE INFORMATION

Locate the data plate on the right side of the unit, above the copper-filtered access
port. The data plate contains the incubator model number and serial number. Enter
this information below for future reference.
Data Plate
Date Plate Information
Model Number
Serial Number

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GRAPHIC SYMBOLS (CONTINUED)

The incubator is provided with multiple graphic symbols on its exterior and internal surfaces. These
symbols identify hazards, and the functions of the adjustable components, as well as important
notes in the user manual.

Symbol Definition

Indicates that you should consult your service manual for further instructions.
Indique que l'opérateur doit consulter le manuel d'utilisation pour y trouver les instructions
complémentaires.

Indicates: Caution hot surface

Indique: Avertissement symbole de surface chaude

Indicates Temperature
Repère température

Indicates the Over Temperature Limit system

Indique le système de dépassement de temperature

Indicates AC Power
Repère le courant alternatif

Indicates I/ON and O/OFF

I repère de la position MARCHE de l'interrupteur d'alimentation
O repère de la position ARRÊT de l'interrupteur d'alimentation

Indicates protective earth ground

Repère terre électrique

Indicates UP and DOWN respectively

Touches de déplacements respectifs vers le HAUT et le BA

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GRAPHIC SYMBOLS (CONTINUED)

Symbol Definition

Indicates Potential Shock Hazard

Signale danger électrique

WEEE Directive compliant logo

Indicates the unit should be recycled (Not disposed of in land-fill)

Indique l’appareil doit être recyclé (Ne pas jeter dans une décharge)

Indicates CO2 Gas

Indique gaz CO2

Indicates carbon dioxide gas content as a %

Indique le gaz carbonique contenu en %

Indicates USB Port

Indique Port USB

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INSTALLATION

AMBIENT CONDITIONS
The SCO6AD is intended for use indoors at room temperatures between 15C and 30C (59F and
86F), at no greater than an ambient 80% Relative Humidity (at 25C / 77F). Allow a minimum of
4 inches (10cm) between the incubator and walls or partitions, and 2 inches (5cm) of clearance
above the top of the incubator for unobstructed airflow.

Operating the unit outside these conditions may adversely affect the incubator temperature
performance, including its effective operating range, stability, and uniformity.

POWER SOURCE
Always position the unit so that the end-user has access to the power cord and can quickly unplug
it from the wall in the event of an emergency.

When choosing a location for the incubator, check that the voltage and ampere requirements on the
incubator data plate match that of the wall power source. The source must be an earth grounded
outlet. Supplied voltage must not vary more than 10% from the data plate rating. Damage to
the incubator may result if supplied voltage varies more than 10%. Use a separate circuit to
prevent loss of product due to overloading or circuit failure.

The SCO6AD comes provided with a 125 volt 9ft 5 in (2.86m) NEMA 5-15P power cord.

The required fuse for the incubator is 12.5 Amps.

The incubator is intended for a 50/60 Hz application at 12 Amps.

Note: Electrical supply to the incubator must conform to all national and local electrical codes.

ENVIRONMENTAL FACTORS
When selecting a location to install the incubator, consider all environmental conditions that can
affect unit temperature performance. For example:

 Proximity to ovens, autoclaves, and any device that produces significant radiant heat

 Heating and cooling ducts, or other sources of fast-moving air currents

 High-traffic areas

 Direct sunlight

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INSTALLATION (CONTINUED)

LIFTING
The incubator should only be lifted by its bottom surfaces using proper heavy lifting machinery such
as, a forklift or pallet jack. Handles and knobs are inadequate for lifting or stabilization. The unit
should be completely restrained from tipping during lifting. Transporting the unit while lifted is not
recommended and may be hazardous. Remove all moving parts, such as shelves and trays, and
secure the door in the closed position prior to lifting or transporting the unit.

LEVELING
Make sure that the incubator is level and stable. Each incubator ships with four leveling feet. Insert
one leveling foot into each of the four holes in the bottom corners of the incubator. Stand the
incubator upright. Then, adjust the foot at each corner until the incubator stands level and solid
without rocking. To raise a foot, turn it in a counterclockwise direction; to lower a foot, turn it in a
clockwise direction.

Note: To prevent damage to the feet when moving the incubator, turn each of the four leveling feet
completely clockwise.

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INSTALLATION (CONTINUED)

CONNECT THE CO2 SUPPLY TO THE INCUBATOR

Note: Always use medical grade CO2. Use of non-medical grade CO2 risks introducing
contaminants into the chamber, may damage the incubator, and will void the incubator’s
warranty. Failure to use the in-line HEPA filter also voids the warranty.

Always install the in-line HEPA filter (Part Number 2800525) provided with the SCO6AD tubing
kit between the gas source and the incubator. The filter is directional. Make sure that it is installed
correctly for the flow direction (see page 41). Failure to install the filter will decrease the lifespan of
the SCO6AD, and risks allowing contaminants into the incubation chamber.

During normal operations the incubator uses only small quantities of CO2. Precise regulation of the
gas input flow is vital for the incubator performance. Always use a two-stage CO2 pressure
regulator. Some single-stage regulators have 2 gauges. Make certain your regulator is a two-
stage regulator.

Connect the CO2 regulator to a medical grade CO2 cylinder or source.

Set the CO2 cylinder regulator or wall source regulator to 15 - 20 Pounds per Square
Inch (psi). Do not exceed 20 psi.

PSI Megapascals Kilopascals Bar

15 - 20 psi 0.103 - 0.137 Mpa 103.42 - 137.89 Kpa 1.03 - 1.378 bar
Please see the Pressure Units Conversion table on page 43 in the Unit Specifications section for
the formulas for converting psi into other units of pressure measurement.

Attach the black 1/8 OD tubing of the tubing kit to the regulator or wall source.

Attached the clear 5/16 OD to the CO2 In Port on the back of the incubator.

Do not open the regulator the CO2 incubator at this time.

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INSTALLATION (CONTINUED)
INSTALLATION CLEANING AND DISINFECTION
If required by your laboratory protocol, clean and manually disinfect the incubator chamber and
shelving components. Cleaning and disinfecting during installation reduces the risk of
contamination. The chamber and shelving were cleaned at the factory, and a decontamination
cycle was run to verify the cycle functionality. However, Sheldon Manufacturing cannot guarantee
that the incubator was not exposed to contaminants during shipping.

Please see the Cleaning and Disinfecting entry on page 39 in the User Maintenance section for
information on how to clean and disinfect without damaging the incubator or its components. A
decontamination cycle will be run as part of the Incubator setup in the Operation section.

DEIONIZED AND DISTILLED WATER

Do not use deionized water to clean or humidify the incubator. Use of deionized water may
corrode metal surfaces and voids the warranty. Sheldon Manufacturing recommends the use of
distilled water in the resistance range of 50K Ohm/cm to 1M Ohm/cm, or a conductivity range of
20.0 uS/cm to 1.0 uS/cm, for cleaning and humidifying applications.

SHELVING INSTALLATION
Remove all protective wrappings from shelving components prior to installing:

Install the shelf standards.

a. Align the keyhole slot of the standard with the mounting

peg on the side of the chamber wall.

b. Mount the shelf standard.

Install the shelf slides.

a. Insert the shelf slide into the shelf standard using a

rocking motion.

b. The shelf slide will sit level when correctly

installed.

Install the shelves.

a. Slide into position with the bent ends facing

up in the back and down in the front.

Install the humidity pan.

a. Place the copper anti-microbial token in the humidity pan.

b. Secure the token using the clip on the bottom of the pan.

c. Place the pan on the chamber floor.

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CONTROL PANEL OVERVIEW

OTL - Data Panel (Left) and Control Panels

OTL - Data Panel

Over Temperature Limit Thermostat (OTL)
Labeled Set Over Temp Control, the OTL section of the panel is provided with a graduated dial.
The OTL system is an independent backup heating control. When set, it guards against the
chamber temperature rising more than 1°C above the main temperature control set point. Please
see the Over Temperature Limit System description in the Theory of Operations (page 21).

OTL Light
Marked Over Temp, this red pilot light illuminates when the Over Temperature Limit system takes
control of the incubator by depowering the chamber heating elements. Under normal operating
conditions this pilot lamp should never turn on. Activation of the light may signal a significant fault
event.

Jacks and Ports

A USB-style serial port for capturing CO2 and temperature data, and a dry contact jack for
outputting alarm activation instances are located on the OTL – Data Panel. The panel is also
provided with a CO2 sample port. Please see the Data Output Capabilities entry in the Operation
section on page 38.

Main Control Panels

Power Switch
The round, black, main power switch controls all power to the incubator and its systems. “I” is the
on position, and “O” the off.

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CONTROL PANEL OVERVIEW (CONTINUED)

Temperature Control and Display Panel

Labeled Set Temperature °C, this panel comes with a green digital display that shows the air
temperature in the incubation chamber, accurate to 0.1°C. The display can also show the user-
selected temperature set point, as well as display-adjustments during calibrations. The controls
consists of an UP / DOWN arrow pad for inputting a temperature set point, muting active
temperature deviation alarms, and performing calibrations. Red LED alarm indicators marked HIGH
and LOW illuminate whenever temperature deviations of ±1°C or greater from the current set point
are detected. The yellow LED marked Mute illuminates whenever an audible alarm is being muted.
See the Muting the Audible Temperature Alarm entry on page 28 of the Operations section for
more information.

The green LED indicator labeled HEATING ACTIVATED illuminates whenever the incubator
temperature control system is heating the incubation chamber.

CO2 Control and Display Panel

Labeled Set CO2, the display on this panel shows the concentration of CO2 in the incubation
chamber as a percentage of the chamber atmosphere, with a range of 0% to 20%. During the
automatic decontamination cycle this display shows messages alerting the user to which stage of
the cycle the unit is in, as well as a timer countdown while in the two-hour heat soak stage. The
panel can also show the CO2 set point, as well as display-adjustments while in gas calibration
mode.

The UP / DOWN arrow pad on this panel is used to input a CO2 concentration set point for the
incubation chamber, mute active gas deviation alarms, and to enter calibration corrections. Red
LED alarm indicators marked HIGH and LOW illuminate whenever CO 2 deviations of ±1% or
greater from the set point are detected. The yellow LED marked MUTE illuminates whenever an
audible deviation alarm component is being muted. See the Muting the Audible CO2 Alarm entry
on page 32 of the Operations section for more information.

Marked CO2 INJECTING, this green LED indicator illuminates when the incubator is injecting CO2
into the incubation chamber.

Decontamination Cycle Panel

This panel is used to initiate or abort the automated decontamination cycle. Its controls also lock
and unlock the door prior to and after the cycle. Unlocking door after completing or aborting the
cycle restores the system to its normal operations mode. The panel is also provided with LED lights
that indicate what stage of the cycle or cycle-abort the incubator is in.

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CONTROL PANEL OVERVIEW (CONTINUED)

Fuse
Located inside the black cord inlet on the back of the incubator, the fuse protects against
overcurrent conditions. If the fuse blows, the incubator will shut down. For your safety, the cause of
a blown fuse should be determined prior to replacing it. Always de-power and unplug the incubator
prior to accessing the fuse holder.

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OPERATION

THEORY OF OPERATION
The SCO6AD is engineered to provide a constant temperature CO2 incubation environment. The
incubator can obtain a stable, uniform (±0.25°C) temperature in its chamber, from the room
temperature +5°C up to 50°C, when properly calibrated.

The incubator operates with a relative humidity (RH) in the chamber of up to 95%. Operating with
RH levels in excess of 95% will result in condensation on the incubation chamber surfaces. Please
see the Condensation and Dew Point entry on page 37 of the Operations section for information
on managing humidity.

The SCO6AD employs proportional-integral-derivative analytical feedback-loop functions when

measuring and controlling temperature and CO2 levels. The rate of PID-controlled heating is
proportional to the difference between the measured chamber air temperature and the user-
programed temperature set point. The rate of heating slows as the air temperature nears the set
point to prevent overshooting. Likewise, the PID-controlled gas injection length is proportional to the
difference between the measured chamber gas concentration and the CO2 set point, and slows as
the chamber atmosphere nears the set point. During normal operations heating pulses and CO2
injections take place in small bursts to correct for deviations of 0.1°C and 0.1% in gas
concentration. Heating pulses and gas injections will be frequent when first setting up the unit for
use after installation, and when recovering from door openings.

The incubator features inner glass viewing door that allows visual inspection of samples without
compromising the chamber CO2 environment. The SCO6AD is also provided with an automated
high-heat decontamination cycle that does not require the removal of the CO2 sensor or other
electronic components.

Heating
The incubator uses solid-state internal temperature probes, a microprocessor controller, and an air-
circulation pump to monitor and regulate air temperature within the incubation chamber. Heating is
provided by elements located in an insulating air jacket space surrounding the chamber on four
sides and beneath the chamber floor. A fan circulates air within the jacket. Additionally, the exterior
chamber door is self-heating to bolster the thermal uniformity and stability of the chamber, and to
minimize condensation on the glass viewing door.

Leaving the exterior door open for long durations may adversely impact the temperature
performance of the incubation chamber. It may also create condensation on the viewing door. Door
openings should be restricted to the minimum necessary to view or access samples in the
chamber.

The incubator relies on natural heat radiation for cooling. With the doors closed, the lowest
chamber temperature that can be sustained is the ambient room temperature plus the waste heat
generated by the unit’s internal electrical and mechanical operations (the room temperature +5C).

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OPERATION (CONTINUED)

The SCO6AD requires periodic calibrations to verify that it is accurately measuring and displaying
the chamber air temperature. If a measurement error is detected during a temperature calibration
procedure (see page 25), the temperature display can be calibrated to the actual temperature by
placing the display in its calibration mode. Temperature calibrations require a reference
temperature sensor accurate to at least 0.1°C.

CO2 Atmosphere
The SCO6AD controls the percentage of CO2 in the incubation chamber atmosphere by operating
an internal electric gas pump connected to the gas input line. The motor pulses CO2 into the
chamber air stream when deviations from the user-programed CO2 set point are detected. The
incubator monitors the chamber CO2 concentration with an infrared sensor in a recirculation box
above the main chamber. The sensor operates on the principle that a specific frequency of infrared
light is absorbed by CO2. The more CO2 present in the air stream from the chamber, the more
infrared is absorbed. The infrared sensor is only sensitive to CO2, so the measurement accuracy
(±0.1%) is consistent, regardless of the presence of other gasses. The incubator can maintain a
CO2 concentration level of 0 – 20% in the chamber, and has a recovery time of less than 5 minutes
to restore the concentration level after the inner chamber door has been closed.

The SCO6AD requires periodic calibrations to verify that it is accurately measuring and displaying
the CO2 level in the chamber. If a measurement error is detected during a CO2 calibration
procedure (see page 30), the CO2 display can be calibrated to the actual concentration in the
chamber by placing the display in its calibration mode. Gas calibrations require a gas-analyzer
reference sensor capable of measuring CO2 concentrations to at least 0.1% accuracy.

Alarms
The SCO6AD is provided with high and low alarms for temperature and CO2 deviations. The
alarms have visual indicators and audible components. The temperature high and low alarm
thresholds are deviations of 1°C or greater from the set point. The CO2 deviation alarm trip points
are deviations of 1% or greater from the gas concentration set point. There is a 15 minute delay
for the sounding of the low deviation audible alarms to prevent activations when the chamber
doors are opened for brief periods. If the low CO2 deviation indicator is lit for 20 minutes, a visual
No Gas Supply alarm will activate and show in the CO2 display as “ngS”.

All audible deviation alarms can be muted, but the visual indicators will remain illuminated for the
duration of a deviation. See the Muting the Audible Temperature Alarm entry on page 28 and the
Muting the Audible CO2 Alarm entry on page 32.

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OPERATION (CONTINUED)

Humidification
Humidification is provided by filling the humidifying pan included with the unit. The pan is then
placed on the heated chamber floor. Evaporation driven in part by heating raises the relative
humidity percentage (RH%) of the chamber. A copper anti-microbial token helps to retard the
growth of microbiological organisms in the pan.

Decontamination Cycle
The SCO6AD comes with a user-initiated eight-hour high heat cycle designed to kill microbiological
organisms. This automated cycle consists of three stages: A 1.5 hour ramp up from room or
incubation temperature to 180°C; a 2 hour soak at 180°C; and a 4.5 hour cool down. Prior to
launching a decontamination cycle the humidity pan should be drained and its copper antimicrobial
token removed to prevent discoloration of the pan.

Physical and Data Access

A copper-filtered access port on the right side of the unit allows sensor and monitoring probes such
as, thermocouples and humidity meter solid state probes, to be inserted and left in the chamber
without compromising the CO2 atmosphere. An atmosphere sample port for independently verifying
the CO2 concentration in the chamber is provided on the Over Temperature Limit System control
panel. This panel also has a contact jack port that activates whenever a temperature or CO2 alarm
is triggered. A USB-style serial port on the same panel is provided for digital capture or monitoring
of temperature and CO2 levels.

The Over Temperature Limit System (OTL)

When set, the OTL system prevents runaway heating in the event of a main control failure by
depowering the heating element whenever the temperature in the incubator chamber exceeds the
OTL setting. Typically the OTL is set 1°C above the temperature display’s set point. Because of its
nature as a cutoff system and its lack of PID analytics, the OTL cannot deliver the same degree of
temperature stability and measurement precision as the digital display and controls. The OTL
System should only be used as a means of heating regulation for the incubation chamber until a
failed controller board can be repaired or replaced.

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OPERATION (CONTINUED)
PREPARING THE INCUBATOR FOR USE
Note: The preparation procedure requires approximately 24 hours to complete. This includes at
least 8 hours for the incubator temperature, humidity, and CO2 levels to stabilize prior to
temperature and gas calibrations, and 8 hours to run a decontamination auto cycle.

Perform the following steps and procedures to prepare the SCO6AD for use each time it is installed
in a new workspace environment:

If you have not done so already, clean and disinfect the chamber and shelving, if required
by your laboratory protocol. Cleaning and manually disinfecting the chamber, shelving
components, and the humidity pan and token will reduce the risk of microbiological
contamination.

a. Always turn off and unplug the SCO6AD when cleaning and manually
disinfecting the unit.

b. Note: An auto decontamination cycle will be run in step 8f.

Verify that the side access port stopper is in place on the outside of the unit.

a. Or, a clean and disinfected thermocouple probe for performing the initial
temperature calibration may be inserted through the access port at this time.

b. See step 1 of the Temperature Calibration procedure on page 25 for the

correct placement of a thermocouple probe.

Verify that the workspace power supply and incubator requirements listed on its data plate
match.

Plug the power cord into the earth grounded electrical outlet.

Prepare the humidity pan for use.

a. Verify that the copper anti-microbial token is secured in the pan.

b. Fill the pan with distilled water. Do not use deionized water.

c. The pan must be filled and in the incubation chamber at this time to
prepare the unit for an accurate temperature calibration.

Open the CO2 gas regulator so it supplies 15 - 20 psi to the incubator. It can take up to 20
seconds for CO2 to reach the gas sensor in the SCO6AD after the regulator is opened and
the incubator is powered up.

Place the Power switch in the ON position.

Note: Powering up a cold SCO6AD with no CO2 present in the chamber may trip the temperature
or CO2 deviation alarms during the Set Up process. To mute the alarms, please see Muting
the Audible Temperature Alarm entry on page 28 or the Muting the Audible CO2 Alarm
entry on page 32.

Procedure continued on next page

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OPERATION (CONTINUED)

Preparation Procedure Continued

Complete the following procedures in the Operation section:

a. Set the Temperature Set Point page 24

b. Calibrate the Temperature Display page 25

c. Set the Over Temperature Limit page 27

d. Set the CO2 Set Point page 29

e. Calibrate the CO2 Display page 30

f. Run a decontamination cycle page 33

End of Preparation Procedure

HUMIDIFYING THE INCUBATOR

Filling the humidity tray with distilled water and placing it on the heated incubator floor will raise the
humidity level of the chamber to approximately 90 – 95% relative humidity.

 Always use the antimicrobial copper token when humidifying the incubator to slow the
growth of microbiological populations in the tray.

 Regularly clean and disinfect tray.

 Refill as needed, and change the water in the tray at least once per week.

 Use of chemical disinfectants added to the tray may alter the surface tension of the water.
This may significantly reduce the rate of evaporation and impact the humidity level of the
incubator chamber.

 Never use deionized water.

 Ensure that the unit is humidified when performing temperature adjustments and
calibrations.

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OPERATION (CONTINUED)
SET THE TEMPERATURE SET POINT
The unit comes from the factory with a set point of 37°C. Perform the steps below to change the set
point to the operational temperature you will be using for your incubation application. This also
prepares the unit for temperature calibration. The example below assumes the incubator will be run
at 39°C during normal use.

Turn the Over Temperature Limit control dial clockwise to the maximum
position indicated by the largest dot.
a. This prevents the Over Temperature Limit system from
interfering with the Set the Temperature Set Point and
Temperature Calibration procedures.

Press either the Up or Down key one time on the Set Temperature
panel to activate the temperature set point mode.
a. The temperature display will briefly flash the letters “SP” to
indicate a Set Point is about to be displayed.
b. The Set Temperature digital display will then show the
flashing, adjustable temperature set point.

Use the Up or the Down arrow key to adjust the flashing set point
to the temperature of your incubation application.
a. If neither key is pressed within 5 seconds the displayed set
point will be saved.

Wait 5 seconds after entering the new set point.

a. The display will stop flashing. The new set point is now saved
in the microprocessor controller.
b. The incubator will now automatically heat or passively cool to
match the new set point.
c. The display will revert to showing the current chamber air
temperature.

Leave the Over Temperature Limit control at the maximum setting if you
will be performing a temperature calibration.

End of Procedure

24 | P a g e
 OPERATION (CONTINUED)
CALIBRATE THE TEMPERATURE DISPLAY
The SCO6AD was calibrated at the factory to 37°C. The incubator should be calibrated as required
by regulatory compliance and your laboratory protocol, or whenever the unit is installed in a new
working environment. Calibrate the incubator at the temperature set point of your incubation. Use
a certified temperature-sensing reference device regularly calibrated to 0.1°C by an independent
party. For best results use a digital reference device with a remote sensing probe such as, a
thermocouple wire probe. A remote probe offers significant time savings by eliminating a 1 hour
wait for the chamber temperature to re-stabilize each time the door is opened to check the
reference device reading. Alcohol thermometers are not suitable for performing calibrations.

Allow the incubator to stand for at least 8 hours undisturbed running at its operational set
point to stabilize prior to calibrating. Stabilized means there are no temperature fluctuations of
±0.1°C or greater for at least 1 hour. The unit must be humidified and supplied with CO2 during
this period. Failure to allow 8 hours will result in an inaccurate temperature calibration. Sheldon
Manufacturing recommends allowing the unit to stabilize overnight for best results.

If you have not already done so, place the temperature sensor of the
reference device inside the incubation chamber as close as possible to the Use the access port
chamber geometric center. Check that the sensor is not in direct contact stopper to block
with the shelving. intrusions of air and
prevent chilling of the
Note: A thermocouple sensor probe sleeve may be taped to the shelving, as long incubator’s atmosphere
as the exposed copper end is 2 inches (5cm) above the shelf. An exposed sensor after introducing a remote
probe in direct contact with the shelving may experience heat sinking, which can probe through the port.
result in an inaccurate temperature reading.

Allow the chamber temperature to re-stabilize each time the chamber door
exterior is opened and closed.
a. The reference device temperature measurement should not
change for at least one (1) hour in order for the chamber to be
considered re-stabilized.

Compare the reference device and the incubator display temperature

readings. If the readings are the same, or the difference between the two
(2) falls within the acceptable range of your laboratory protocol, the
incubator is calibrated for temperature. The Temperature Calibration
procedure is complete.

If there is a difference between the two readings, and that offset falls
outside the acceptable range your laboratory protocol, place the incubator
into calibration mode. See the next step.
a. Allow the temperature to re-stabilize if the exterior door has
been opened.

25 | P a g e
 OPERATION (CONTINUED)

Temperature Calibration (Continued)

Place the display in temperature calibration mode.

a. Press and hold both the UP and DOWN arrow buttons
simultaneously.
b. The Temperature Display will show the letters “CO”, then
begin flashing the current temperature value.
c. Release the arrow buttons.
Display with Adjusted Value
d. Use the Up and Down arrows individually to adjust the current
temperature value until it matches the reference device
temperature reading. This will correct the measurement error.
e. If an arrow key is not pressed for five seconds, the
Temperature Display will cease flashing, and store the last
displayed value as the new current chamber temperature
value.

After correcting for the error, wait five (5) seconds.

a. The incubator Temperature Display will cease flashing and
store the correction.
b. The incubator will now begin heating or allow itself to cool in
order to reach the set point with the corrected display value.

Allow the incubator sit for 1 hour undisturbed to stabilize after it

has achieved the temperature set point. Failure to wait until the
unit is fully stabilized will result in an inaccurate reading. See next
step.

Compare the reference device reading with the incubator temperature

display.
a. If the reference device and the incubator temperature display
readings are the same or the difference falls within the range
of your laboratory protocol, the incubator is now calibrated
for temperature.


If the two readings are not the same or fall outside your laboratory
protocol, repeat steps 5 – 8.

Procedure continued on next page



26 | P a g e
OPERATION (CONTINUED)
Temperature Calibration Continued
If the temperature readings of the incubator and the reference device still fall outside your
laboratory protocol after three calibration attempts, contact Sheldon Technical Support
for assistance.

Note: At least three attempts may be required to sucessfully calibrate a unit that is more than 2°C
out of calibration.

End of Procedure

SET THE OVER TEMPERATURE LIMIT

The incubator must be operating at the user-selected set point temperature, and must be stable for
at least one hour prior to setting the OTL. Perform the following steps to set up the Over
Temperature Limit system for use:

Example

If you have not done so already, turn the Set Over Temperature Limit
control dial clockwise to the maximum position.

Turn the Over Temperature Limit control dial counterclockwise until the
red Over Temp Limit Activated light illuminates.

Slowly turn the dial clockwise until the Over Temperature Limit
Activated light turns off. Stop turning the control.

Leave the OTL dial set slightly above the activation point. The Over
Temperature Limit is now set approximately 1˚C above the current
chamber air temperature.

End of Procedure

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OPERATION (CONTINUED)

MUTING THE AUDIBLE TEMPERATURE ALARM

An audible and visual high or low deviation alarm will activate if the incubation chamber
temperature deviates by 1°C above or below the temperature set point. The low deviation audible
alarm has a delay of fifteen minutes. This prevents the low alarm from sounding whenever the
doors are opened, causing a temporary drop in temperature.

To mute an active high or low deviation alarm, press and hold either the Up or
Down arrow on the Temperature Control panel, until the amber Mute LED
illuminates and the audible alarm shuts off.

The audible alarm component will remain muted for the duration of the current
temperature deviation. The visual alarm indicator will remain illuminated.

Any new deviation of ±1°C or greater will reactivate the audible alarm.

28 | P a g e
 OPERATION (CONTINUED)

SET THE CO2 SET POINT

After the temperature has been calibrated and the Over Temperature Limit set, enter the CO2 set
point (concentration percentage) of your incubation application. The incubator comes from the
factory with a set point of 5%.

The gas supply must deliver 15 - 20 psi to the throughout this procedure

Note: The visual example below represents adjusting the CO2 set point from 4 to 5%.

Press either the Up or Down arrow button on the CO2 panel.

a. The display will flash the letters “SP” for set point.
b. A flashing, adjustable CO2 set point will appear in the display.

Set point adjusted to 5%.

Adjust the flashing set point using the arrow buttons.
a. If the buttons are not pushed for five (5) seconds, the display
will revert to showing the current chamber CO2 level.
b. The last set point value in the display will be saved as the new
CO2 set point.

After selecting a CO2 set point, wait five (5) seconds. Controller injecting CO2 to
a. The display will cease flashing. achieve the new set point.
b. The display will revert to showing the current concentration
percentage in the chamber.
c. The new set point is now saved in the microprocessor
controller.
d. The incubator will now begin adjusting to reach the set point.

Note: The CO2 display can be set to off when entering a new set point. Hold the
down arrow until the display reads “OFF”. The incubator will cease injecting CO2,
and the gas deviation alarms will not activate until a new set point is entered.

End of Procedure

29 | P a g e
OPERATION (CONTINUED)

CALIBRATE THE CO2 DISPLAY

Note: The incubator must be stabilized at its calibrated operational temperature and CO2 set points
before the CO2 display can be accurately calibrated. The incubator must supplied with a feed
of 15 – 20 psi of CO2 throughout this procedure.

Always use a CO2 reference sensor regularly calibrated to a concentration accuracy of 0.1%. For
best results use a digital gas analyzer with a sample collection tube.

The incubator should be calibrated at the concentration level it will be set to during your incubation
application.

Wait until the unit has been stabilized at its CO2 set point for one hour with no changes in
concentration on the CO2 display panel before starting this procedure. Failure to wait until the unit is
stabilized will prevent an accurate calibration from being carried out.

Connect a digital CO2 analyzer sample tube to the sample port, located on
the OTL control panel.

Measure the current CO2 percentage (%) inside the chamber with the
reference sensor.
a. Compare the reference reading with the percentage (%)
shown on the incubator CO2 display panel.
b. If the readings are the same, or fall within the acceptable
range of your laboratory protocol the incubator is now
calibrated for CO2. The Calibrate CO2 Display procedure is
complete. 

If there is a difference between the two (2) readings, and that offset falls
outside the acceptable range of your laboratory protocol, place the CO2
display into calibration mode. See the next step.

Procedure continued on next page

30 | P a g e
 OPERATION (CONTINUED)

Calibrate the CO2 Display (Continued)

Place the display into calibration mode by pressing and holding both the
CO2 control Up and Down arrow buttons simultaneously.
a. The letters “CO” will appear, followed by current display value,
which will flash continually.
b. Release the arrow buttons.
c. Use the Up and Down arrows individually to adjust the
flashing value so that it matches the reading of the reference
CO2 analyzer.
d. If an arrow button is not pressed for 5 seconds, the display will
cease flashing and store the last shown value as the new
chamber concentration. To re-enter calibration mode press
and hold both the up and down arrow buttons.

After correcting for the measurement error, wait five seconds.

a. The CO2 display will cease flashing and store the correction.
b. The incubator will now begin injecting CO2 or allowing the
concentration in the chamber to decay to reach the set point
with the corrected display value.

Allow the incubator sit for one (1) hour undisturbed to stabilize after it has
achieved the CO2 set point with the corrected display value.
Failure to wait until the chamber atmosphere is fully stabilized will
result in an inaccurate reading during the next step.

Compare the reference device reading with the incubator CO2 display.
a. If the reference device and the incubator display readings are
the same, or the difference falls within the acceptable range of
your laboratory protocol, the incubator is now calibrated for
CO2.

If the two readings are not the same or fall outside your laboratory protocol
range, repeat steps 5 – 8.
a. Repeat for a total of three attempts.

Procedure continued on next page

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OPERATION (CONTINUED)

CO2 Calibration Continued

If the gas CO2 concentration readings of the incubator and the reference device still fall
outside your laboratory protocol after three calibration attempts, contact Sheldon
Technical Support for assistance.

Note: Three attempts may be required to sucessfully calibrate a unit that is more than 2% out of
calibration.

End of Procedure

MUTING THE AUDIBLE CO2 ALARM

Visual high or low deviation indicator alarms will activate if the incubator CO2 level deviates 1%
above or below the CO2 set point. An audible alarm will sound immediately for a high deviation.
The low deviation audible alarm will sound after the visual low indicator alarm has been
continually illuminated for fifteen (15) minutes. This delay prevents the alarm from sounding
whenever a door opening creates a short-lived drop in gas concentration.

To mute an alarm, press and hold the CO2 Up or the Down arrow button for one (1)
second.

The alarm will stay muted for the duration of the current temperature deviation, but the
yellow Muted indicator will remain illuminated.

Another deviation of 1% will reactivate the audible alarm.

NO GAS SUPPLY ALARM (NGS)

If the Low Gas deviation indicator is active for longer than twenty (20) minutes, a
second alarm will activate. The letters “ngS” will appear in the CO2 display to
indicate No Gas Supply. The alarm will remain active even if the incubator is turned
off and turned back on. The NGS Alarm will remain on until CO2 is restored to the
chamber. It may take several minutes of CO2 inflow to establish a concentration
percentage (%) high enough to deactivate the alarm.

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OPERATION (CONTINUED)

DECONTAMINATION CYCLE
Note: The infrared CO2 sensor is protected from exposure to high temperatures during the
decontamination cycle. It does not need to be removed at any point during normal operations,
including decontamination.
Note: The incubator will automatically cease injecting CO2 for the duration of the cycle.

An instructional video for how to launch the decontamination auto cycle in the SCO6AD
(previously designated as the 3552) can be found on the Shel Lab website at:
http://www.shellab.com/store/virtual-tour-3552-co2.html.

The incubator was run through a decontamination cycle at the factory. However, Sheldon
Manufacturing cannot guarantee that unit has not been exposed to contaminants en route.

Remove all samples and equipment.

Leave all shelving items inside the chamber, if allowed by your laboratory protocol.

Empty the humidity tray of all water.

a. Failure to empty the tray will extend the ramp up heating and cool down stages
of the decontamination cycle. This may adversely impact the cycle’s efficacy.

Remove the copper antimicrobial token from the humidity pan.

a. Leaving the token in the pan during a decontamination cycle will result in
oxidation of the token, and possible discoloration or staining of the humidity
pan.

b. Manually disinfect the token using disinfectants and cleaning methods

appropriate to your laboratory protocol.

Close both the internal and external incubator chamber doors.

a. The decontamination cycle will not initiate while the doors are open.

Procedure continued on next page

33 | P a g e
 OPERATION (CONTINUED)

Decontamination Cycle (Continued)

Lock the chamber doors.

a. Press and hold the Operation Selector Switch on the
Decontamination Cycle display sub-panel until the amber
Decon Cycle indicator begins flashing.
b. The doors will lock with an audible mechanical noise.
c. The doors must be locked before the decontamination cycle
can be initiated.

Launch the decontamination cycle.

a. Press and hold the Decontamination Cycle Switch until the
amber Decon Cycle and red Decon Activated indicators flash
alternatingly.
b. The green Normal Operation indicator will extinguish.

Ramp up stage.
a. The cycle is now active.
b. The Decon Cycle and Decon Activated light will continue to
flash alternatingly while the incubator ramps up to 180°C.
c. The CO2 display will show the letters “dEC” to indicate that the
decontamination cycle is running.
d. Under normal conditions the ramp up will take 1.5 hours (90
minutes). However, the presence of additional mass such as,
water in the humidity pan, may extend the ramp up period as
there is more material that must be brought to temperature.

Soak stage.
a. When the incubator chamber air temperature has reached
180°C, the Decon Cycle and Decon Activated lights will
remain on continuously.
b. The CO2 display will show a countdown, starting at 120
minutes.
c. The 2 hour soak period is required to kill most microbiological
organisms.

Procedure continued on next page

34 | P a g e
 OPERATION (CONTINUED)

Decontamination Cycle (Continued)

Cool down stage.

a. When the CO2 display reaches 0, the display will show the
letters “CdN”. This indicates that the cycle has entered the
cool down stage.
b. The amber Decon Cycle and green Normal Operation
indicators will flash alternatingly throughout the cool down.

The cool down period will end the chamber air temperature reaches 50°C.
a. The CO2 display will show the letters “PAS” when the cycle
has concluded.

Restore the incubator to normal operations mode by unlocking the door.

a. Press and hold the Operation Selector switch until the
amber Decon Cycle light extinguishes, and the green Normal
Operation indicator remains on continuously.
b. The CO2 display will return to showing the CO2 concentration
in the chamber.
c. The door will unlock, accompanied by an audible mechanical
sound.

End of procedure

35 | P a g e
OPERATION (CONTINUED)
ABORTING THE DECONTAMINATION AUTO CYCLE
To abort the decontamination cycle perform the following steps:

Press and hold both the Decontamination Cycle Switch and the Normal
Operation Switch for approximately 4 seconds.
a. The CO2 display will show the letters “Ab” to indicate that
SCO6AD has successfully aborted the cycle.
b. The Decon Activated indicator light will extinguish, though the
Decon Cycle light will remain illuminated.

The CO2 display will then show the letters “Cdn”.

a. This indicates that the incubator is cooling down.
b. The amber Decontamination Cycle and green Normal Operation
indicators will flash alternatingly throughout the cool down stage.
c. The door will remain locked during the cool down stage. This
is a safety feature to safeguard against injuries caused by
exposure to hot chamber surfaces and shelving.

The cool down period will end the temperature reaches 50°C.

a. The CO2 display will show the letters “PAS” when the abort
has concluded.

Unlock the door and return the SCO6AD to normal operations.

a. Press and hold the Operation Selector Switch.
b. The CO2 display will return to showing the concentration level
in the chamber.
c. The door will unlock, accompanied by an audible mechanical
sound.
d. The amber Decon Cycle indicator will deactivate. The green
Normal Operation light will illuminate.

End of procedure

36 | P a g e
OPERATION (CONTINUED)

CONDENSATION AND THE DEW POINT

Relative humidity inside the incubator chamber should never be allowed to exceed 95%.
Exceeding this thresholds will likely result in condensation, possible leaks around the incubator,
and may cause corrosion damage if allowed to continue for any significant length of time

Condensation appears wherever the humidity level in the incubator chamber reaches the dew
point. The dew point is the level of humidity at which the air cannot hold more water vapor. The
warmer the air, the more water vapor it can hold.

As the level of humidity rises in an incubation chamber, condensation will first appear on surfaces
that are cooler than the air temperature. Near the dew point, condensation will form on any item or
exposed surface that is even slightly cooler than the air. When the dew point is reached,
condensation forms on nearly all exposed surfaces.

Managing condensation primarily depends on either lowering the humidity level or increasing the air
temperature in the incubator chamber.

Note: Rising or falling air pressure from weather will adjust the dew point up and down in small
increments. If the relative humidity in the incubation chamber is already near the dew point,
barometric fluctuations may push it across the dew point threshold.
Note: Thin air at higher altitudes holds less humidity than the denser air found at or near sea level.

If condensation is forming in the incubator chamber, check the following:

 Is the access port stopper properly seated in the port on the outside of the unit? The
SCO6AD depends on a controlled atmospheric leak rate through its copper-filtered access
port to help prevent condensation. Stopping up the port by placing the stopper in the port
inside the chamber will interfere with this leak rate.

 Are the interior and external chamber doors closing and latching properly? Are the door
gaskets leaking? Check each gasket for damage, wear, or signs of brittleness or dryness.
Replace gaskets if needed.

 Are frequent or lengthy chamber door openings causing significant temperature

disruptions? If so, reduce the number of openings. Utilize the glass viewing door when
conducting visual inspections of samples.

 Is the incubator exposed to an external flow of cold air such as, an air-conditioning vent or
a door to a cooler hallway or adjacent room? Block or divert the air, or move the incubator.

 Does the ambient humidity in the room exceed the stated operating range of 80% relative
humidity? If so, lower the room humidity.

 Are there are too many open containers of evaporating sample media in the chamber? If
so, reduce the number of sample containers.

 Remove the humidity pan or other open containers of water.

37 | P a g e
OPERATION (CONTINUED)

LOADING THE INCUBATOR

Place items on the shelves inside the incubation chamber as evenly spaced as possible. Good
spacing allows for maximum air circulation and a high degree of temperature uniformity. Leave 1
inch (2.5cm) between sample containers and the chamber walls.

ACCESSORY COMPATIBILITY
Make sure that any accessory equipment used inside the incubation chamber can safely and
effectively operate within your selected temperature range, RH%, and CO 2 level. Some equipment
types such as, stirrers or shakers, may generate heat sufficient to disrupt the thermal uniformity and
stability of the chamber.

DATA OUTPUT CAPABILITIES

The SCO6AD generates data outputs describing CO2 percentages and temperature levels.
These outputs are transmitted through a USB-style serial port located on the OTL – Data
Control panel, on the front left of the incubator. A software driver and data logging package
for the port can be downloaded from the Shel Lab website. The driver software is required for use
of the port. To download the software, visit the product page of the SCO6AD incubator and click
on the large USB bar icon titled “USB Software for CO2 Incubators” located approximately halfway
down the page.

http://shellab.com/product/sco6ad-high-heat-decontamination-co2-incubator-5-9-cu-ft/

A dry contact jack port for communicating alarm activation instances can be connected to a building
management system. The alarm port is located next to the USB-style port. The jack port accepts
standard audio jacks available from most electronics retailers. These are also known as ¼ inch 2-
pole audio connectors or phono jacks. A jack is not included with incubator.

38 | P a g e
USER MAINTENANCE
Warning: Prior to any maintenance or cleaning of this unit, disconnect the power cord from the power
supply.
Avertissement: Avant d'effectuer toute maintenance ou entretien de cet appareil, débrancher le
cordon secteur de la source d'alimentation.

CLEANING AND DISINFECTING

If a hazardous material or substance has spilled in the incubator, immediately initiate your site’s
Hazardous Material Spill Containment protocol. Contact your local Site Safety Officer and follow
instructions per the site policy and procedures.

The incubator chamber should be cleaned and disinfected prior to first use. Periodic cleaning and
disinfection are required to prevent microbiological contamination.

Do not use spray on cleaners or disinfectants. These can leak through openings and coat electrical
components. Do not use cleaners or disinfectants that contain solvents capable of harming paint
coatings or stainless steel surfaces. Do not use chlorine-based bleaches or abrasives; these
will damage the chamber liner.

Warning: Never clean the unit with alcohol or flammable cleaners.

Avertissement: Ne jamais nettoyer l'appareil à l'alcool ou avec des nettoyants inflammables.

Cleaning
Remove all non-attached chamber components and accessories (shelves, racks, and any
additional items), if present.

Clean the chamber interior with a mild soap and water solution, including all corners.

Take special care when cleaning chamber temperature probes heads located at the rear of
the chamber on the left side.

Clean all removable accessories and components. The humidity pan should be cleaned
frequently.

Clean and disinfect any attached sample tubing and replace if discoloring is present.

Rinse the chamber surfaces and shelving with distilled water and wipe dry with a soft cloth.
Do not use deionized water. See the Distilled and Deionized Water paragraph in the
Installation section.

39 | P a g e
USER MAINTENANCE (CONTINUED)

Disinfecting
Disinfect the incubation chamber on a regular basis. For maximum effectiveness disinfection
procedures are typically performed after cleaning. Perform the following steps to manually disinfect
the incubator:

Turn the unit off. Open all doors and carryout your laboratory, clinical, or production space
disinfection protocol.

Disinfect the incubation chamber using commercially available disinfectants that are non-
corrosive, non-abrasive, and suitable for use on stainless steel surfaces. If disinfecting
external surfaces use disinfectants that will not damage painted metal or plastic. Contact
your local Site Safety Officer for detailed information on the disinfectants compatible with
your cultivation or culturing applications.

If possible, remove all interior accessories (shelving and other non-attached items) from the
chamber when disinfecting.

Disinfect all surfaces in the chamber, making sure thoroughly disinfect the corners.
Exercise care to avoid damaging the temperature sensor probes.

Gas concentrations from evaporating disinfecting agents can inhibit growth or metabolic
symptoms in microbiological sample populations. Make sure that chlorines, amphyls,
quaternary ammonias, or any other overtly volatile disinfecting agents have been rinsed or
otherwise removed from the chamber surfaces, prior to placing samples in the chamber.

MINIMIZING CONTAMINATION EXPOSURE

The following are suggestions for minimizing exposure of the incubator chamber to potential
contaminants.

 Maintain a high air quality in the laboratory workspaces around the incubator.

 Avoid placing the incubator near sources of air movement such as, doors, air vents, or high
traffic routes in the workspace.

 Minimize the number of times the incubator chamber doors are opened during
normal operations.

 Placing the unit on a rolling caster platform will facilitate cleaning of the floor
around the unit. If possible, move floor seated units out of the space prior to
cleaning the floor there. See the Accessories section on page 46 to order a
castor platform from Sheldon Manufacturing.

40 | P a g e
USER MAINTENANCE (CONTINUED)

HEPA FILTER AND GAS LINES “IN”

Sheldon Manufacturing recommends replacing the external in-line gas HEPA filter once
per year, or more often when the filter is noticeably discolored. The filter is directional,
and must be installed facing in the correct direction. The word “IN” is stamped on the rim
of the filter assembly on the side that faces toward the gas source.

Gas lines should be replaced when cracking, brittleness, permanent kinking, or other signs of
damage are present. Please see the Parts List on 44.

STORAGE OF THE SCO6AD INCUBATOR

Perform the following steps if the incubator will be out of use for more than 24 hours to prevent
microbiological contamination such as, fungus or mold. Disinfect the unit before drying if the
chamber and shelving have been exposed to pathogenic microorganisms, or if required by your
laboratory protocol.

Depower the incubator.

Empty and dry the humidity pan.

Use a soft cloth to dry the chamber surfaces.

The incubator will not need to be recalibrated for temperature and CO 2 if it is returned to
operation in the same workspace environment it was last calibrated in — unless changes
have been made in the ambient conditions.

DOOR AND ELECTRICAL COMPONENTS

Inspect the door latch, trim, catch, and gasket for signs of deterioration every six months. Failure to
maintain the integrity of the door components shortens the lifespan of the incubator.

Electrical components do not require maintenance. If the SCO6AD fails to operate as specified,
please contact your Shel Lab dealer or distributor or Shel Lab Technical Support for assistance.

41 | P a g e
UNIT SPECIFICATIONS
The SCO6AD High Heat Auto-Decontamination Incubator is a 100–120 voltage unit. Please refer to
your incubator’s data plate for individual electrical specifications.

Technical data specified applies to units with standard equipment at an ambient temperature of
25°C and a voltage fluctuation of ±10%. The temperatures specified are determined in accordance
to factory standard following DIN 12880 respecting the recommended wall clearances of 10% of the
height, width, and depth of the inner chamber. All indications are average values, typical for units
produced in the series. We reserve the right to alter technical specifications at all times.

WEIGHT
Shipping Net Weight
285 lbs. / 129 kg 237 lbs. / 100 kg

DIMENSIONS
By inches

Exterior W × D × H Interior W × D × H
28.5 x 30.8 x 39.5 inches 20.2 x 20 x 25.5 inches

By centimeters

Exterior W × D × H Interior W × D × H
72.4 x 78.2 x 100.4 cm 51.4 x 50.8 x 64.7 cm

Access Port

Diameter
1.5 inches (3.8 cm)

CAPACITY
Cubic Feet Cubic Liters
5.9 167

42 | P a g e
UNIT SPECIFICATIONS (CONTINUED)

CO2
Range Accuracy Recovery Time
0 – 20% ± 0.1% Less than 5 minutes

TEMPERATURE
Range Uniformity Stability
Ambient +5ºC to 50ºC ± 0.25ºC at 37ºC ± 0.1C @ 37C

POWER
Model AC Voltage Amperage Frequency
SCO6AD 110 – 120 12.0 50/60 Hz

CONVERSION TABLE

43 | P a g e
PARTS LIST
COMPONENTS
Description Parts Number Description Parts Number

Power Cord 125 volt 9ft 5 in

Access Port Stopper
(2.86m) NEMA 5-15P

Brass Tubing Fitting (Gas

Tubing Kit Component) Shelf Slide

Door Gasket Inner Shelf Standard

Door Gasket Exterior (Sold

Stainless Steel Shelf
by the foot. Requires 9 feet)

5121777

HEPA Filter for CO2 Gas Tubing Black 1/8 OD

Line (Tubing Kit Component) (Gas Tubing Kit Component)

Tubing Clear 5/16 OD

Humidity Pan
(Gas Tubing Kit Component)

Humidity Pan Copper

Gas Tubing Kit Complete
Antimicrobial Token

Leveling Feet

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PARTS (CONTINUED)

ORDERING PARTS AND CONSUMABLES

If you have the Part Number for an item, you may order it directly from Sheldon Manufacturing by
calling 1-800-322-4897 extension 3. If you are not certain that you have the correct Part Number, or
if you need that specific item, please contact Sheldon Technical Support for help at 1-800-322-4897
extension 4 or (503) 640-3000. Please have the model number and serial number of the
incubator ready, as Tech Support will need this information to match your unit with its correct part.

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ACCESORIES

Shel Lab offers the following accessories for the SCO6AD

Caster Platform
A rolling platform for the SCO6AD. Allows for greater ease of transportation
and cleaning of floor space.

Part Number 9000574

CO2 Cylinder Regulator

Part Number 7150509

Compressed Gas Cylinder Switcher, Automatic

Allows two gas cylinders to be connected to one gas port, and automatically switches
from the first to the second cylinder when the first is empty.

Part Number 2002-B

Copper Shelf Assembly

Three antimicrobial copper shelves. Includes six copper shelf slides.

Part Number: 9750582 complete assembly described above.

PN 5820504 Individual Shelf PN 5820505 Individual Slide

Stacking Stand
A stacking stand for two SCO6AD incubators

Part Number 9000575

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