Professional Documents
Culture Documents
APT - Line CB (E3) : Service Manual
APT - Line CB (E3) : Service Manual
APT.line CB (E3)
CO2 incubators
with FPI-sensor system and display controller MB1
Service Manual
Imprint
Manufacturer:
BINDER GmbH
Address P.O. Box 102
D-78502 Tuttlingen
Phone +49 7462 / 2005 0
Fax +49 7462 / 2005 100
Internet http://www.binder-world.com
E-mail info@binder-world.com
Service Hotline +49 7462 / 2005 555
Service Fax +49 7462 / 2005 93 555
Service E-mail service@binder-world.com
Service Hotline USA +1 866 816 8191
Service Hotline Asia Pacific +603 6204 2855
Table of contents
Table of contents......................................................................................................................... 4
1 Introduction ................................................................................................................... 6
1.1 General guidelines .......................................................................................................... 6
1.2 Syntax ............................................................................................................................. 6
1.3 Structure of the safety instructions................................................................................... 7
4 Maintenance ................................................................................................................ 13
4.1 Safety check acc. to DIN VDE 0701-0702 ..................................................................... 16
5 Troubleshooting .......................................................................................................... 17
5.1 General electrical faults ................................................................................................. 18
5.2 Faults of the heating system .......................................................................................... 21
5.3 Faults of the CO2 system ............................................................................................... 23
1 Introduction
1.1 General guidelines
This service manual describes maintenance, trouble shooting, repair, calibration and adjustment
of the CB series.
Sections only concerning 230 V, 115 V or 100 V units or units with options are indicated.
The figures in this service manual show the service work on the CB150 (230 V). The service work
of other CB units is to perform analogical.
This service manual is aimed at service personnel who should maintain, repair, calibrate or adjust
a CB.
References about the required qualification of the service personnel can be found in chap. 2.1 on
page 9.
The service manual of the CB series will be updated if necessary.
Always use the latest version of the service manual.
In case of doubt about the validity of this service manual please contact the Binder Service Hotline
(see Imprint).
Wiring diagrams are published separately. The required wiring diagrams can be ordered at the
Binder Service Hotline.
This service manual is according to the wiring diagrams for the units:
CB 053 (E3), 230 V, 9040-0069, 56005001.PRO
CB 053 (E3), 100 - 120 V, 9040-0070, 56005501.PRO
CB 150 (E3), 230 V, 9040-0038, 56015004.PRO
CB 150 - UL (E3), 115 V, 9040-0042, 56015505.PRO
CB 150 (E3), 100 V, 9040-0048, 56015502A.PRO
CB 210 (E3), 230 V, 9040-0039, 56025001.PRO
CB 210 - UL (E3), 115 V, 9040-0043, 56025502.PRO
CB 210 (E3), 100 V, 9040-0041, 56025502A.PRO
All information about initial operation, normal operation, cleaning, alarm and error messages
of the CB units with controller MB1 can be found in the relevant “operating manual” delivered with
the CB.
1.2 Syntax
Syntax Meaning
(-1A1) Marking of electrical equipment and electric contacts
<button> Button to be pushed
"text" Displayed text or text to be keyed in
Warning levels
Dangers are indicated, according to the severity and likelihood of their consequences,
by a word to attract attention, with the associated warning color and any appropriate
safety symbol:
DANGER!
Indicates an imminently hazardous situation that, if not avoided, will result in
death or serious (irreversible) injury.
WARNING!
Indicates a potentially hazardous situation which, if not avoided, could result in
death or serious (irreversible) injury.
CAUTION!
Indicates a potentially hazardous situation which, if not avoided, may result in
moderate or minor (reversible) injury.
CAUTION!
Indicates a potentially hazardous situation which, if not avoided, may result in damage of the
product and/or its functions or of a property in its ambiance.
Danger of injury.
Follow all the directions indicated by the safety symbol in order to avoid injury
or death.
Stability hazard
Danger of scalding
Important information
WARNING!
If the CB is maintained or repaired incorrectly, there is a risk of malfunction which
could cause harm to human beings and material damage to the equipment and
samples.
CAUTION!
If the CB is calibrated or adjusted incorrectly, there is a risk of malfunction which could cause
material damage to the samples and false test results.
To be able to carry out the service work the enforcing personnel must be qualified and
familiar with the service and the operation of the CB.
Sufficient qualification is achieved by:
Electro technical training
Technical training by BINDER GmbH
Knowledge of the present service manual
Knowledge of the operating manual of the CB to be serviced
Experience in servicing environmental simulation chambers for constant climates
2.2 Dangers
DANGER!
Danger of electric shock.
Danger to life.
The unit must be switched off and disconnected from the power supply prior to
performing most of the described service tasks.
Take all precautionary measures that a unit which is disconnected from the
power supply will not be inadvertently connected to the power supply.
The unit must be live to perform some of the service tasks.
In this case, a second person must be present who is able to switch off the unit
in case of emergency.
CAUTION!
CAUTION!
Danger of damaging the electronics.
Circuit boards might be damaged by handling malpractices and touching of its
components.
Prior to work at electronic components the technician must take appropriate
protective measures against electrostatic discharge. Wearing ESD shoes and
a grounding bracelet have shown to be useful.
Before opening the triangle instrument box the technician should at least
electrostatically discharge by touching grounded metallic objects.
Prior to work at the electrical equipment check identity of the components with
the aid of the wiring diagram.
The assembly of the electrical equipment may be different from the description
in this service manual.
CAUTION!
Danger of mechanical damages.
Never let mechanical components hang at electric cables. Electric cables are not
appropriate to hold bigger components and will be damaged if you do so.
WARNING
Harmful substance. Danger of poisioning.
Biohazard. Danger of infection.
Due to the use the unit may be contaminated with harmful, infectious or
radioactive substances.
You shall not service a unit with sticking harmful or radioactive substances or
sources of infection.
You shall not service a unit before the form “Declaration of harmlessness” is
completely filled out.
Prior to any service tasks, the operating staff shall free the unit from any
sticking harmful or radioactive substances or sources of infection.
Prior to any service tasks, a person in charge of the operating staff shall fill
out the form “Contamination clearance certificate”.
Incubators series CB are designed for cultivation of mammal cells under typical conditions of
about 37°C (see chap. “Intended use” of the „Operating manual”).
Due to the use by the operating company it might be possible that the unit is contaminated with
harmful, infectious or radioactive substances inside and outside.
For the safety of the service personnel prior to any service tasks the unit shall be freed from
any sticking harmful or radioactive substances or sources of infection by the operating staff.
Prior to any service tasks, a person in charge of the operating staff shall fill out the form
“Contamination clearance certificate”.
For the “Contamination clearance certificate” refer to the operating manual.
4 Maintenance
WARNING
Hazardous voltage
Risk of electric shock
The check for electrical safety shall be done during the recommended
annual maintenance work.
The check for electrical safety shall be done after every repair.
Nobody shall operate the unit if the check for electrical safety fails.
Service personnel shall look for possible causes of the fail and repair them.
All mechanical and electrical parts of the units should be visually checked, tested and
maintained by qualified service personnel (chap. 2.1, page 9) annually.
Inner chamber
Glass door gasket Check the glass door gasket for wear, tight fit and damages
If necessary, replace the glass door gasket (chap. 6.4, page 29)
Glass door, Check the glass door, the door hinges and the latch catch for
latch catch damages
Check the strength of the adhesion between the glass door and the
hinges resp. the handle
Check the adjustment of the glass door
Check the smooth-running of the glass door
If necessary, adjust the glass door and the latch catch
(chap. 6.8, page 33)
If necessary, replace the glass door (chap. 6.5, page 30)
Inner chamber Check the inner chamber for mechanical damages and corrosion
If necessary, contact the BINDER service hotline.
Machine room
Machine room Check the machine room for fouling, mechanical damages and
corrosion
If necessary, clean the machine room from dust and similar
impurities
If necessary, check by what mechanical damages and corrosion
are caused and eliminate these causes
Check the water pan for tightness and deposit.
Electric and electronic
Power plug Check the power plug and the power cord for damages
Power cord If necessary, replace the power cord with the power plug
(chap. 7.7, page 55)
Electric mounting plate Check electric mounting plate for tight fit, fouling, mechanical
damages and corrosion
Electric components of the Check the electric components of the electric mounting plate for tight
electric mounting plate fit, fouling, mechanical damages and corrosion
Check the cables being connected to the electric components of the
electric mounting plate for sufficient fixing and damages
If necessary, replace electric components of the electric mounting
plate (chap. 7, page 48)
Fix loose cables and replace damaged cables
CO2 system
Gas fine filters Replace all gas fine filters
(chap. 7.4, page 51)
Hoses Check all hoses of the CO2 inlet system and the CO2 sensor system
for tight fit, obstructions and leakages.
If necessary, replace a hose
Controller
Controller display (-3N1), Check the function of the buttons and the displays by performing
LEDs different inputs (see „Operating manual”)
If necessary, replace the controller display (chap. 6.12, page 37)
Controller Check the function of the controller by entering different menus
(see „Operating manual”)
If necessary, replace the controller MB1 I/O-board (chap. 7.11,
page 59)
Set point setting Key in several set points for temperature and check if the unit starts
approaching this values (see „Operating manual”)
Execute a troubleshooting, if the unit does not start approaching
the set points (chap. 5, page 17)
Temperature control, Calibrate the temperature control (chap.10.2, page 84)
CO2 control If necessary, adjust the temperature control
If necessary, replace the Pt100 temperature sensor (-3B1)
(chap. 8.8, page 71)
Calibrate the CO2 control (chap.Fehler! Verweisquelle konnte nicht
gefunden werden., page Fehler! Textmarke nicht definiert.)
If necessary, adjust the CO2 control
If necessary, replace the CO2 sensor board (chap. 7.15, page 63)
Safety checks
Check for electrical safety * Check the unit for visible damages and defects.
(acc. to DIN VDE 0701-0702) Check the protective conductor resistance and the insulation
resistance as well as the protective conductor current
(chap. 4.1, page 16).
If one of the checks fails, the unit shall not be operated.
Look for the possible causes and repair it.
Operating manual Check if the operating manual is available (in particular for safety
instruction)
* According to general technical rules, conduct a safety check acc. to DIN VDE 0701-0702 after
every repair or modification of a unit.
Alternatively you can perform the safety check also with similar national standards if they contain
the tests described in chap. 4.1, page 16.
Independent of the standard used for the safety check, the measurement values indicated in
chap. 4.1, page 16 must be reached.
WARNING
Hazardous voltage
Risk of electric shock
The check for electrical safety shall be done during the recommended
annual maintenance work.
The check for electrical safety shall be done after every repair.
Nobody shall operate the unit if the check for electrical safety fails.
Service personnel shall look for possible causes of the fail and repair them.
During the yearly maintenance and following repair we recommend an inspection acc. to
DIN VDE 0701-0702. This inspection comprises the following points:
1. Visual inspection
Check the unit and all electrical connecting lines visible damages and defects.
Replace damaged parts and electrical connecting lines.
2. Measuring the protective conductor resistance
The protective conductor resistance is measured between the grounding contacts
of the power plug and several different, electrically conductive spots of the housing.
The measuring current for measuring the resistance must be at least 0.2 Amp.
The protective conductor resistance must not exceed the value 0.3 Ω.
Possible causes of error: corroded connections, no electrically conductive
measuring spots due to lacquer or dirt.
Cause removal: Clean connections or make the measuring spots metallically bare.
3. Measuring the insulation resistance
The insulation resistance is measured between one conduct and several different,
electrically conductive spots of the housing. All switches must be closed during the
measurement.
The resistance must not fall below the value 0.3 MΩ.
When measuring, make sure that the unit is not dirty or wet. If the measured
resistance value is too low, we recommend drying thoroughly the unit and repeating
the measurement.
4. Measuring the protective conductor current
The protective conductor current is the current that flows through the protective
conductor when the unit is switched on. All switches must be closed during the
measurement.
Set the following set-point value:
Temperature: +60°C / 194°F
The measurement is effected at all possible plugging positions of the power plug.
The protective conductor current must not exceed 3.5 mA. If the current is
exceeded, a possible cause may be bad insulation or short-circuit of a coil.
Coils exist e.g. in fans, power supply units, motors, solenoid valves, and relays.
By temporary electrical uncoupling of the corresponding parts, you can identify
and localize possible sources of error.
5 Troubleshooting
Alarm and error messages as well as basic measures the operator himself
should take to clear them are described in the “CB operating manual”.
Please make sure these measures have been tried before looking further for
any other possible error causes.
Error pattern
No
Faults should be found by replacing components of the unit. Usually that will be the fastest method
of troubleshooting.
The sequence of the columns describing potential causes for errors, and the remedial actions to
clear the described error patterns, is mainly intended to facilitate basic error diagnosis and
clearance.
So it is recommended to work your way from top to bottom of the diagrams.
Notations between brackets and in italics, e.g. (-1S1), behind a component refer to the respective
equipment codes for this component, as specified in the wiring diagram, and in labels on some
parts inside the unit.
Yes
Power plug (-X1) not connected Connect power plug
No
Yes
Power switch (-1S1) set to OFF Set power switch to ON
No
Yes
Facility-side power supply not available Ensure facility-side power supply
No
Plugs not connected to the power Yes Connect plugs to the power switch
switch (-1S1) (chap. 6.11, page 36)
No
No
No
No
Controller cable (-X60) not connected to Yes Connect controller cable (-X60)
the controller display (-3N1)? (chap. 6.12, page 37)
No
Controller cable (-X60) not connected to Yes Connect controller cable (-X60)
the controller MB1 I/O-board (-3N2)? (chap. 7.11, page 59)
No
The alu flex tube for the Permadry™ water Yes Put on the alu flex tube airtight
pan at the bottom of the inner chamber is
(chap. 7.5, page 52)
not put on
No
Fan for the Permadry™ water pan (-4M2) Yes Replace fan for the Permadry™ water
defective pan, (chap. 7.9, page 57)
No
The heating wire (-4E3) inside the door is Yes Check and replace the heating wire
defective. if necessary (chap. 6.15, page 39)
No
CO2 sensor (-2B1) not plugged or fixed Yes Plug or fix the CO2 sensor tightly
tightly. (see fig. 39).
No
DANGER
Hazardous voltage
Risk of electric shock
You shall switch off the unit and disconnect it from the facility-side power
supply if this is instructed in the description of a service task.
You shall disconnect the unit from the facility-side power supply to make
sure that it cannot be switched on again by mistake.
CAUTION
Burn hazard.
Hot surface inside.
You shall allow the unit to cool down below 36° C before servicing.
A B C D E F
When screwing in the door holder lug unnecessarily far (left-hand rotation) the
adjusting screw works loose from the thread of the door holder lug, and the internal
spring may fall into the housing.
3. Align the door holder lug (A) in a way that the closing of the door can seal easy-running in the
openings of the door holder lug and the door closes tightly all around.
4. Test if the door is proper adjusted and closes tight by opening and closing it.
1. Pull the old door gasket (A) from the edges of the interior side (C) of the outer door.
2. For ease of mounting loosen the screws of the interior side a little (chap. 6.14, page 38).
3. Put the new gasket (A) on the upper edge (C) of interior side of the outer door
(see and ).
The joint at which the gasket is cemented should be located at that side where the door
hinges are located.
4. Push the door gasket (A) with its groove (B) on the upper edge of the interior side of the
outer door (C).
5. Pass the door gasket further on the lateral edge and push the groove (B) on the edge.
6. Pull the door gasket downward diagonally until it can be pushed completely on all edges.
7. Test if the door closes tight by opening and closing it (chap. 6.1, page 26).
A 4
C A Door gasket
B Groove
B C
A
1. Hold on to the glass door (E) and unscrew the screws (F) of both door hinges.
2. Replace the glass door and screw the new door tightly at its hinges.
3. Unscrew the screws (D) of the latch catch (C).
4. Remove the latch catch (C) and its spacer (B).
5. Replace the latch catch (C) and screw it tightly.
6. Adjust the glass door (E) and the latch catch (C) (chap. 6.8, page 33).
A
B
C D
E
F
The measuring port in the glass door is provided with spacer studs to
avoid underpressure (pressure difference) in the inner chamber.
Thus, the port also serves as a pressure compensating valve.
The part is used from serial no. 07-23805.
Check for any service or maintenance if the correct part is used in the
glass door (fig. 5).
If necessary replace, the measuring port.
fig. 5: Checking and changing the measuring port at the glass door
C Multi frame
1. If the multi frame (C) is not in straight position, it can be adjusted with the elongated holes
of the hinge (B). The multi frame must be precisely aligned and close smoothly.
2. Check the gasket of the multi frame (C) on the chamber for wear, tight fit and damages.
If necessary, replace the gasket.
3. Check the gaskets (D) of the single glass door (A) for wear, leaks and damage.
If necessary, replace the complete single glass door (A) with glued gasket.
G
F Screws of hinges
G Hinge
C
C Latch catch
A B C D E F G H I
Necessary
Phillips-tip screwdriver size 2
tools:
CAUTION!
Danger of mechanical damages.
Never let mechanical components hang at electric cables. Electric cables are
not appropriate to hold bigger components and will be damaged if you do so.
Please note that demounting the interior side of the outer door is only necessary if,
while demounting the instrument box, the fixing screws have fallen into the door
frame!
5. Pull the instrument box (I, fig. 7) carefully from the outer door.
6. Disconnect the plugs from the power switch (J, fig. 7).
7. Disconnect the controller cable (-X60) (K, fig. 7) from the socket (E, fig.
7) of the
controller MB1 monitor (D, fig. 7).
8. Disconnect the cables from the spring terminals.
9. Remove the instrument box (I, fig. 7).
10. Install the instrument box by following steps 1 to 9 in reverse order.
The plugs of the power switch (J, fig. 7) can be connected to the switch in any
order.
The cables of the spring terminals can be connected to the terminals in any order.
A C
Necessary
tools: Phillips-tip screwdriver size 2
A B C D E
CAUTION!
Danger of mechanical damages.
Never let mechanical components hang at electric cables. Electric cables are
not appropriate to hold bigger components and will be damaged if you do so.
6.15 Testing and replacing the interior side of the outer door
with the heating wire
Spare parts: CB 053 (230 V)
interior side of outer door (order no. 8003-0185)
CB 053-UL (100 V - 120 V)
interior side of outer door (order no. 8003-0187)
CB 150 (230 V)
interior side of outer door (order no. 8003-0060)
CB 150 UL (115 V), CB 150 (100 V)
interior side of outer door (order no. 8003-0077)
CB 210 (230 V)
interior side of outer door (order no. 8003-0163)
CB 210 UL (115 V)
interior side of outer door (order no. 8003-0164)
Shrink hose (UL+CSA) (order no. 6007-0029)
6. Measure the resistance of the heating wire between the connectors (D) by using a multimeter.
CB (230 V)
If Rmeasured = 352 Ω ±10 %, the heating wire is all right.
If Rmeasured ≠ 352 Ω ±10 %, the heating wire is defective, go on with chap. 6.16.
CB UL (100 V - 120 V)
If Rmeasured = 88 Ω ±10 %, the heating wire is all right.
If Rmeasured ≠ 88 Ω ±10 %, the heating wire is defective, go on with chap. 6.16.
Heating wire 230 V /150 W for CB unit 230 V (order no. 5005-0170)
Heating wire 115 V /150 W for CB 053 UL, CB 150 UL (order no. 5005-0171)
Spare part:
Heating wire 115 V /150 W for CB 210 UL (order no. 5005-0173)
Aluminum duct tape 40 mm / 50 m (order no. 6007-0028)
1. Remove the defective heating wire from the interior door and scrape off the old aluminum
duct tape with a hand scraper.
2. Lay the new heating wire as shown in fig. 11.
3. Fix the heating wire with aluminum duct tape.
4. Mount the interior door.
A Interior door
B Heating wire
C Connecting of the heating wire
A B C D
13. Install the new closing device by following steps 1 to 12 in reverse order.
During assembling push rod lever (N) and axle bearing (L) pay attention that the guiding
hooks of the push rod lever are within the guiding nuts of the axle bearing and that the
handle (G) can be turned into door closed position (I) and door opened position (II).
A B C D E F G H I J K L M N O
A B C D E
fig. 14: Replacing the Pt100 temperature sensor of the outer door
A Interior side of the outer door D Cable of the Pt100 temperature sensor
B Aluminum duct tape E Door hinge
C Pt100 temperature sensor (-3B3 )
After replacing the Pt100 temperature sensor adjust the temperature control,
(chap 10.2, page 84).
B
A A Circlip
B Hinge pin
C
C Bearing sleeve
D Outer door
H Ground wire
D
I Connectors for power switch
9. Lift the outer door (D) until it can be removed from the lower door hinge (J).
10. Place the outer door (D) carefully in front of the unit.
11. Unscrew the screws (K) of the hinge support (L).
12. Move the hinge support (L) from the corner to the middle of the door (D).
13. Pull all cables and wires (E, F, G, H, I) through the hinge support (L).
The controller cable (G) should be the last one.
14. Install the outer door by following steps 1 to 13 in reverse order.
15. Adjust the outer door (chap. 6.2, page 26).
A B C L D
J K E F G H I
CAUTION!
Danger of damaging the electronics.
Danger of damages by electrostatic discharge (ESD)
Circuit boards might be damaged by handling malpractices and touching of its
components.
Prior to work at electronic components the technician must take appropriate
protective measures against electrostatic discharge. Wearing ESD shoes and a
grounding bracelet have shown to be useful.
Before opening the triangle instrument box the technician should at least
electrostatically discharge by touching grounded metallic objects.
Prior to work at the electrical equipment check identity of the components with
the aid of the wiring diagram.
The assembly of the electrical equipment may be different from the description
in this service manual.
Necessary
slotted screwdriver
tools:
A Fuse holder
B Miniatur fuse
B
A
E
D
C
B fig. 18: Dismantling the faceplate
A
A Screws of the faceplate D Cover plate
B Faceplate E CO2 hoses from the gas fine filter
C Screws of the cover plate
1. Disconnect the unit from the power supply and dismantling the faceplate (F).
2. Pull the filter upwards out of the support (D).
3. Pull the hoses (C) from the connectors (B)
of the filter (A).
4. Disinfect the inside of the fabric hoses with
disinfectant SOLARSEPT.
5. Wait a few minutes until the fabric hoses
have been dried from the disinfectant.
6. Install the new filter by following steps
1 to 3 in reverse order.
A F
E
A Gas fine filter
B Hose jointer B
C Gas hoses
D Support of the gas fine filter C
E Screws
F Faceplate D
A Faceplate
B Cover plate
Y
/|\ a Z X
W
\|/
V
A T
B
S
C
D
R
E
Q
I
P
J O
K L M N
fig. 21 shows the electric mounting plate of the CB 053 with options.
Please note that the assembly can be different depending on the size,
option and expansion stage.
The shroud (O) is needed to protect the alu flex tube (\|/) from the connections
of the controller MB1 I/O-board. Be sure that the shroud is proberly mounted
when fixing the electric mounting plate.
For more details see the corresponding wiring diagram, the spare part list
for the electric mounting plate and/or the respective electric componet marks.
D A
E
A Terminal block D Cable holder
B Screws of power cord holder E Fixing clip
C Power cord
Necessary
Phillips-tip screwdriver size 2
tools:
B
C
A Screws
B Funnel box B
C Axial fan
binary input 1
binary input 2
binary input 3
binary input 4
93 - 263 V AC
1 2 3 4 5 6 7 8 PE N L1
1 grounding pin
2
AA2 main fan
3
E1 Pt100 input +1 4
interior control- -2 BA2 binary output 2
sensor 3 5
+4
E2 Pt100 input -5 6
in the air jacket BA3 door heating
6 7
12 +11
+13 BA4 heating chamber
E5 Input 0-10 V -14 - 12
15 + 13
reserve 16 BA5 CO2 solenoid valve
- 14
ATTENTION Interface
Display Board 5 6
Please note
the settings of
the jumpers ! Interface RS-422 RS-485 / LON-Bus
Repair work at the electric mounting plate
Repair work at the electric mounting plate
3. Mark all plugs all cables connected to the controller MB1 I/O-board (C) with their positions at
the connectors of the board.
4. Disconnect all plugs and cables from the connectors of the controller MB1 I/O-board.
5. Unscrew the screws (A) which fix the board at the electric mounting plate.
7. Install the new controller MB1 I/O-board by following steps 1 to 6 in reverse order.
8. Adjust the temperature and the CO2 control (chap. 10, page 82).
A Screws
B Cable for MB1 monitor (-X60)
C Controller MB1 I/O-board
6. Remove the manometric switch (B) from the electric mounting plate.
B
D
A
A Connectors C Nut
B manometric switch (-5F1) D CO2 hoses
B
D
A
CAUTION!
Wear protective gloves against cutting injuries during mounting the rear
panel because the sheet metal components are sharp-edged.
B Fan cover
C Rear panel
10 cm A
5 cm
D maintenance plate
A B C D E F
H D
6 cm 4 cm
18 cm
A Connector cables
B Screws
C Temperature fuse
6 cm
E
F G H
A B C D E
11. Unscrew the screws (E) from the sensor support (D).
12. Remove the support (D) from the Pt100 sensor (C).
13. Pull the Pt100 sensor and its connector cable (A) trough the cable bushing (B).
14. Install the new Pt100 temperature sensor by following the steps 1 to 13 in reverse order
without testing the sensor.
15. Be sure all cables and connectors are on the right position.
16. After mounting the plate of the outer chamber insulate the rear panel with rock wool.
17. Overlap and seal the rock wool with aluminum duct tape accurately.
18. Adjust the temperature control (chap. 10.2, page 84).
After replacing the Pt100 temperature sensor adjust the temperature control,
(chap 10.2, page 84).
8.7 Example for the assembly of the gas sensors and nozzles
A
B
C
D
E
F
G
fig. 38: Gas sensors and nozzles H
E1 +1 white
probe in the interior -2 blue
(control sensor) 3 blue
E6 1 white blue
probe safety controller 2 red blue
(safety controller) 3 red
7. Compare the measured resistance values with the values given in Table 4 regarding the
measurement uncertainties of the thermometer and the multimeter.
8. If the measured value divides more than 10 % from the value in Table 4, the Pt 100
temperature sensor seems to be defective and should be replaced.
Table 4: Temperature-resistance table of Pt100
T (°C) 0 1 2 3 4 5 6 7 8 9 10
-10 96.086 96.478 96.870 97.262 97.653 98.045 98.436 98.827 99.218 99.609 100.000
0 100.000 100.391 100.781 101.172 101.562 101.953 102.343 102.733 103.123 103.513 103.902
10 103.902 104.292 104.681 105.071 105.460 105.849 106.238 106.627 107.016 107.404 107.793
20 107.793 108.181 108.570 108.958 109.346 109.734 110.122 110.509 110.897 111.284 111.672
30 111.672 112.059 112.446 112.833 113.220 113.607 113.994 114.380 114.767 115.153 115.539
40 115.539 115.925 116.311 116.697 117.083 117.469 117.854 118.240 118.625 119.010 119.395
50 119.395 119.780 120.165 120.550 120.934 121.319 121.703 122.087 122.471 122.855 123.239
60 123.239 123.623 124.007 124.390 124.774 125.157 125.540 125.923 126.306 126.689 127.072
70 127.072 127.454 127.837 128.219 128.602 128.984 129.366 129.748 130.130 130.511 130.893
80 130.893 131.274 131.656 132.037 132.418 132.799 133.180 133.561 133.941 134.322 134.702
90 134.702 135.083 135.463 135.843 136.223 136.603 136.982 137.362 137.741 138.121 138.500
100 138.500 138.879 139.258 139.637 140.016 140.395 140.773 141.152 141.530 141.908 142.286
9. Remove the plate of the outer chamber (chap. 8.5, page 68).
10. Loosen all fasteners and lacing cords of the Pt100 cable in the machine room.
11. Unscrew the nut (A) from the Pt100 support (D), the nut (A) is located inside the inner
chamber.
F
E G
A Nut of Pt100 support
A
E O2/N2 injection nozzle
G CO2 sensor
B A
C
D Pt100 support
12. Pull the Pt100 temperature sensor (C) and its support (D) out of the rear panel (B) of the inner
chamber.
13. Install the new Pt100 temperature sensor by following steps 1 to 0 in reverse order
without testing the sensor.
14. Overlap and seal the rock wool with aluminum duct tape accurately.
15. Adjust the temperature control (chap 10.2, page 84).
A B H
A Screws 3,9x9,5 Note: With CB 053 units the direction of the screws A is from inside
B Support to outside at the top and at the bottom of the support.
H Fan
7. Unscrew the cap nut (E) and remove the shim washer (D) and the fan impeller (C).
8. Unscrew the self locking nuts (F) and pull out the fan motor (H) from the assembly plate (J).
9. Make sure that the vibration damper are fixed correctly at the assembly plate (J).
10. Mount the fan by following steps 1 to 8 in reverse order.
11. At last seal the edges of the support with aluminum duct tape
C D E
B
L
K
J
I
H
CAUTION!
Danger of cutting injuries by sharp edges
Cutting hazards
Be careful when pulling the fan support (B) out of the assembly plate (J).
You should wear safety gloves.
CB 053, 115V / 230 V units: ribbed heating 115 V / 400 W (order no. 5005-0187)
CB 150, 115V / 230 V units: ribbed heating 115 V / 550 W (order no. 5005-0137)
Spare parts: 100V units: ribbed heating 100 V / 550 W (order no. 5005-0141)
CB 210, 115V / 230 V units: ribbed heating 115 V / 650 W (order no. 5005-0138)
100V units: ribbed heating 100 V / 650 W (order no. 5005-0142)
1. Disconnect the unit from the power supply and remove the rear panel (chap. 8.1, page 64).
2. Mark the connection plugs (B) of the heating elements (C).
3. Pull back the silicon insulation hoses (D) from the connecting plugs (B).
4. Disconnect the connecting plugs (B) from the connectors (A) of the heating elements.
5. Measure the resistance of each heating element as shown in fig. 43 by using a multimeter.
CB 150,100 V units:
If Rmeasured = 18 Ω ±10 %, the heating element is all right.
If Rmeasured ≠ 18 Ω ±10 %, the heating element is defective. → Go on with step 6.
CB 210,100 V units:
If Rmeasured = 15 Ω ±10 %, the heating element is all right.
If Rmeasured ≠ 15 Ω ±10 %, the heating element is defective. → Go on with step 6.
A B C
A Connector
B Connecting plug
C Heating element
D Silicon insulation hose
D C B
F A
fig. 44: Replacing the heating elements
6. Dismantle the plate of the outer chamber (chap. 8.5, page 68).
7. Unscrew all the screws (A) und remove the parts (B, C) from the fan panel (D).
8. Unscrew all screws roundabout the fan panel (D) and remove it.
9. Take off the locking washers (E) from the heating element (F) and remove it.
10. Install the new heating element by following steps 1 to 9 in reverse order.
DANGER
Hazardous voltage, Risk of electric shock
Avoid inaccurate insulation of the heating elements connectors.
After the connecting of the heating elements you shall cover the heating
elements connectors with the silicon insulating hose accurately.
9 Spare parts
Name of spare part Order no. Installation
Repair work at the doors chap. 6 , page 26
Door holder lug 8009-0427 chap. 6.2.2, page 27
Outer door gasket 6005-0017 chap. 6.3 , page 28
Glass door gasket CB 053 6005-0187 chap. 6.4 , page 29
Glass door gasket CB 150 6005-0077 chap. 6.4 , page 29
Glass door gasket CB 210 6005-0080 chap. 6.4 , page 29
Glass door gasket for divided glass door CB 150 6005-0102 chap. 6.7 , page 32
Glass door gasket for divided glass door CB 210 6005-0103 chap. 6.7 , page 32
Glass door gasket for single door for divided glass door CB 150 6005-0201 chap. 6.4 , page 29
Glass door gasket for single door for divided glass door CB 210 6005-0202 chap. 6.4 , page 29
Measuring port 6002-0060 chap. 6.6 , page 31
Glass door complete CB 053 8010-0089 chap. 6.5 , page 30
Glass door complete CB 150 8010-0019 chap. 6.5 , page 30
Glass door complete CB 210 8010-0020 chap. 6.5 , page 30
Divided glass door (Add-on kit) CB 150 8012-0580 chap. 6.7 , page 32
Divided glass door (Add-on kit) CB 210 8012-0581 chap. 6.7 , page 32
Latch catch 6002-0124 chap. 6.5 , page 30
Spacer for latch catch 6002-0148 chap. 6.5 , page 30
Caps 6002-0008 chap. 6.9 , page 34
Controller MB1 display (-3N1) 5016-0060 chap. 6.9 , page 34
Connection cable 10 cores with plug for MB1 monitor - 2.5 m (-X60) 5023-0051 chap. 6.9 , page 34
Switch mat 3-fold 6002-0185 chap. 6.9 , page 34
Switch mat 5-fold 6002-0184 chap. 6.9 , page 34
Filter pane 6002-0160 chap. 6.9 , page 34
Instrument box housing 6002-0180 chap. 6.13, page 37
Power switch (-1S1) 5019-0019 chap. 6.11, page 36
Instrument box complete 8008-0119 chap. 6.10 , page 35
Interior side of the outer door CB 053 (230 V) 8003-0185 chap. 6.15, page 39
Interior side of the outer door CB 053 (100 V - 120 V) 8003-0187 chap. 6.15, page 39
Interior side of the outer door CB 150 (230 V) 8003-0060 chap. 6.15, page 39
Interior side of the outer door CB 150-UL (115 V), CB 150 (100 V) 8003-0077 chap. 6.15, page 39
Interior side of the outer door CB 210 (230 V) 8003-0163 chap. 6.15, page 39
Interior side of the outer door CB 210 UL (115 V) 8003-0164 chap. 6.15, page 39
Shrink hose (UL+CSA) 6007-0029 chap. 6.15, page 39
Heating wire 230 V /150 W CB units 230 V 5005-0170 chap. 6.16, page 40
Heating wire 115 V /150 W CB 053 UL, CB 150 UL 5005-0171 chap. 6.16, page 40
Heating wire 115 V /150 W CB 210 UL (115 V) 5005-0173 chap. 6.16, page 40
Door switch 250 V / 25W (-3S1) 5019-0009 chap. 6.17, page 41
Closing device set CB 053 8009-0498 chap. 6.18, page 42
Closing device set CB 150 8009-0073 chap. 6.18, page 42
Closing device set CB 210 8009-0075 chap. 6.18, page 42
Washer D 5,3 3003-0003 chap. 6.18, page 42
O-ring, black 3004-0002 chap. 6.18, page 42
Temperature sensor 2x Pt100 (-3B1) triple wire, straight 5002-0043 chap. 8.8, page 71
Temperature sensor 2x Pt100 (-3B1) triple wire, straight CB 053 UL 5002-0050 chap. 8.8, page 71
Temperature sensor Pt100 (-3B2) bend off 5002-0022 chap. 8.6, page 69
Temperature sensor Pt100 (-3B2) bend off CB 053 UL 5002-0049 chap. 8.6, page 69
Cable bushing 6005-0109 chap. 8.6, page 69
Radial fan 230 V / 50 Hz (-4M1) CB units 230 V 5013-0021 chap. 8.9, page 73
Radial fan 115 V / 60 Hz (-4M1) CB units UL 5013-0047 chap. 8.9, page 73
Fan repair set for 230 V CB units 230 V 5013-0060 chap. 8.9, page 73
Fan repair set for 115 V CB units UL 5013-0061 chap. 8.9, page 73
Support for fan 4022-0030 chap. 8.9, page 73
Miscellaneous
Shelf , perforated, stainless steel 6004-0080
Water pan CB 053 4022-0260
Water pan CB 150, CB 210 4022-0081
Mini pressure reducer 6009-0070 chap. 10.1.1, page 83
Test gas 100% Nitrogen 6017-0003 chap. 10.1.1, page 83
Test gas 80% Oxygen 6017-0002 chap. 10.1.1, page 83
Test gas 5% Carbon Dioxide 6017-0001 chap. 10.1.1, page 83
Calibration and adjustment case 9501-0007 chap. 10.1.1, page 83
Cleaning kit, pH-neutral detergent, disinfectant spray, lint-free tissues 8012-0503
Aluminum duct tape 40 mm / 50 m 6007-0028
Pressure reducer Fail Safe 8009-0232
Hose set for gas connection 8009-0092
Gas hose transparent 6/12 mm 6008-0050
CO2 / FPI 2 sensor head 5002-0023
CO2 / FPI sensor cable 2,5 m 5023-0155
O2 sensor with controller board 5002-0015
CAUTION!
If the CB is calibrated or adjusted incorrectly, there is a risk of malfunction which could cause
material damage to the samples and false test results.
WARNING
Danger of damages to samples beeing incubated in the unit and
faulty test results
This unit shall only be calibrated and adjusted by trained service personnel
(see chap. 2.1, page 9).
You shall read and understand the chap. 10, "Calibration and adjustment of
the CB” of this service manual before calibrating and adjusting.
Before starting the calibration and adjustment of the CO2 control ensure that
the setting of the altitude of the installation site above sea level is correct
(see chap. 0, page 97).
Failure to follow calibration and adjustment instructions could result in damage
to samples beeing incubated in the unit and faulty test results.
The temperature and CO2 control of the unit shall be calibrated and if necessary
adjusted annually and after the following repair works have been done:
Replacing the controller MB1 I/O-board (-3N2) (chap. 7.11, page 59):
- Adjustment of temperature and CO2 control, (chap 10, page 82)
Replacing the Pt100 temperature sensor (-3B1) (chap 8.8, page 71),
replacing the Pt100 temperature sensor (-3B2) (chap 10, page 68),
replacing the Pt100 temperature sensor (-3B3) (chap 6.19, page 44)
- Adjustment of temperature control, (chap 10.2, page 84)
Replacing the CO2 sensor board (-2N1) (chap. 7.15, page 63)
- Adjustment of CO2 control, (chap 10.3.2, page 87)
For CO2 calibration/adjustment: plastic hose (order no. 6008-0020), BINDER mini pressure
reducer with flow meter (usable for all kind of test gases),
BINDER minican test gas 100% Nitrogen,
BINDER minican test gas 5% Carbon Dioxide.
Note: The BINDER service can also use larger pressure gas bottles with analyzed test
gases and a different pressure reducer and flow meter.
Mini pressure reducer Test gas 100% Test gas 5% Test gas 80%
(order no. 6009-0070) Nitrogen Carbon Dioxide Oxygen
(order no. 6017-0003) (order no. 6017-0001) (order no. 6017-0002)
Entries:
Normal Display Config Configuration 2 System-Code 2802 Service Level
Inp. Val. correct. Analogue input 1
Enter the display value of the controller (e.g. 37,0 °C) to End Value X.
Enter the display value of the reference instrument (e.g. 36,5 °C) to End Value W.
Set State to On
Adjustment of the CO2 controller: Determination and entry of the adjustment values
Starting Situation
The CO2 incubator is equilibrated to the humidity adjustment value (e.g. X = W = 5 vol.-%).
The reference instrument shows a value (e.g. 4.7 vol.-%).
Reading out the actual values
Normal Display Config Configuration 2 System-Code 2802 Service Level
Analogue Inputs read out and note 4. (momentary actual value)
Entries
Normal Display Config Configuration 2 System-Code 2802 Service Level
Inp. Val. correct. Analogue input 4
Enter the display value of the controller (5 vol.-%) to End Value X.
Enter the display value of the reference instrument (e.g. 4.7 vol.-%.) to End Value W.
Set State to On.
Leave the menu with 4 x EXIT.
The corrected value appears on the controller display after 5 seconds.
After exposing the sensor 5 minutes to each test gas, note down the CO2 concentration
given on the MB1 controller. An adjustment of the CO2 controller is only necessary if a
deviation between the CO2 concentration shown on the MB1 controller and the value given
on the test gas bottle exceeds +/-0.2 vol.-% (confidence criteria).
Adjustment of the CO2 controller at 5 vol.-% CO2: entry of the adjustment values
Starting Situation
The sensor has been exposed 5 minutes to the CO 2 test gas (5 vol.-% CO2). Exposure will
be continued during value entry at the controller (adjustment).
After 5 minutes exposing of the sensor to each test gas, enter the CO 2 concentration of the
gas to the MB1 controller according to following description.
Entries
Normal Display Config Configuration 2 System-Code 2802 Service Level
Inp. Val. correct. Analogue input 4
Enter the display value of the controller (e.g. 4.7 vol.-%.) to End Value X.
Enter the CO2 concentration of the CO2 test gas (5.0 vol.-%) to End Value W.
Set State to On
Leave the menu with 4 x EXIT.
The corrected value 5 vol.-% appears on the controller display after 5 seconds. Afterwards,
stop the exposure with test gas.
Entries
Normal Display Config Configuration 2 System-Code 2802 Service Level
Inp. Val. correct. Analogue input 4
Enter the display value of the controller (0 vol.-%) to Start Value X.
Enter the display value of the reference instrument (e.g. 0.3 vol.-%.) to Start Value W.
Leave the menu with 4 x EXIT.
Entries
Normal Display Config Configuration 2 System-Code 2802 Service Level
Inp. Val. correct. Analogue input 4
Enter the display value of the controller (5 vol.-%) to End Value X.
Enter the display value of the reference instrument (e.g. 4.7 vol.-%.) to End Value W.
Set State to On.
Leave the menu with 4 x EXIT.
The corrected value appears on the controller display after 5 seconds.
After exposing the sensor 5 minutes to each test gas, note down the O2 concentration
shown on the MB1 controller. An adjustment of the O2 controller is only necessary if a
deviation between the O2 concentration shown on the MB1 controller and the value given
on the test gas bottle exceeds +/-0.2 vol.-% (confidence criteria).
Starting Situation
The sensor has been exposed 5 minutes to ambient air (20.9 vol.-% O2). Exposure will be
continued during value entry at the controller (adjustment).
After 5 minutes exposing of the sensor, enter the O 2 concentration of the ambient air to the
MB1 controller according to following description.
Entries
Normal Display Config Configuration 2 System-Code 2802 Service Level
Inp. Val. correct. Analogue input 4
Enter the display value of the controller (e.g. 18.0 vol.-%.) to End Value X.
Enter the O2 concentration of the ambient air (20.9 vol.-%) to End Value W.
Set State to On
Leave the menu with 4 x EXIT.
The corrected value 20.9 vol.-% appears on the controller display after 5 seconds.
Afterwards, stop the exposure with test gas.
Proceeding:
In normal display, press the “menu” button to enter
the CB-Menu.
Enter the user code: (factory setting = 00001)
Enter the altitude value in meters [m] into the field
“Enter setpoint”.
Confirm with ENTER and leave the menu with EXIT.