Report for tissue number T-351

Date of collection 27 FEB 2023

Test Date Report
HIV I&II 27 FEB 2023 Negative
HIV PCR 01 MAR 2023 Negative
HCV Antibody 27 FEB 2023 Negative
HBsAg NAT 27 FEB 2023 Negative
Syphilis RPR 27 FEB 2023 Negative
Culture test 14 MAR 2023 Sterile

Release Date 14 MAR 2023 Expiry Date 26 FEB 2024

Dr. Suryakant Hayatnagarkar M.D.(Path)

It is certified that AMT Graft tissue is procured from healthy donors undergoing elective caesarean section and
placental membranes are harvested in sterile atmosphere. Amnion is further processed and sterilized using
antibiotic bath in tissue culture media to clean and remove cells and retain natural properties of basement
membrane. Finally preserved in tissue culture media, antibiotics and glycerol and stored between 0C to -20C.

Every batch of media and the tissues stored are cultured and are sterile.

Procedure for preparing tissue for use

1. The AMT Graft tissue is delivered in double packed plastic vials. Outer surface of outer vial should be
cleansed using Isopropyl alcohol and allowed to dry.
2. Using aseptic precautions outer vial is opened and inner sterile vial is removed. The tissue number
should be checked before removing tissue.
3. Carefully open the inner vial and retrieve the membrane filter with the AMT graft using sterile forceps
and drop in a sterile saline bowl.
4. It should be washed using 3 changes of sterile saline before using.
5. There are 2 surfaces to the AMT graft membrane- Epithelial surface which is shiny seen under
operating microscope and it does not stick to sterile cotton swab stick and matrix surface which is dull
and sticks and curls onto sterile swab stick.
6. Depending on operative requirement surfaces should be oriented as required.
7. Please ensure suitability of received graft for the purpose procured. Do not use when found
defective or not as per your specifications.
 Report for tissue number T-351
Date of collection 27 FEB 2023
Test Date Report
HIV I&II 27 FEB 2023 Negative
HIV PCR 01 MAR 2023 Negative
HCV Antibody 27 FEB 2023 Negative
HBsAg NAT 27 FEB 2023 Negative
Syphilis RPR 27 FEB 2023 Negative
Culture test 14 MAR 2023 Sterile

Release Date 14 MAR 2023

Expiry Date 26 FEB 2024

Dr. Suryakant Hayatnagarkar M.D.(Path)

It is certified that AMT Graft tissue is procured from healthy donors
undergoing elective caesarean section and placental membranes are
harvested in sterile atmosphere. Amnion is further processed and
sterilized using antibiotic bath in tissue culture media to clean and
remove cells and retain natural properties of basement membrane.
Finally preserved in tissue culture media, antibiotics and glycerol and
stored between 0C to -20C.

Every batch of media and the tissues stored are cultured and are sterile.

Procedure for preparing tissue for use

1. The AMT Graft tissue is delivered in double packed plastic vials.
Outer surface of outer vial should be cleansed using Isopropyl
alcohol and allowed to dry.
2. Using aseptic precautions outer vial is opened and inner sterile
vial is removed. The tissue number should be checked before
removing tissue.
3. Carefully open the inner vial and retrieve the membrane filter
with the AMT graft using sterile forceps and drop in a sterile
saline bowl.
4. It should be washed using 3 changes of sterile saline before using.
5. There are 2 surfaces to the AMT graft membrane- Epithelial
surface which is shiny seen under operating microscope and it
does not stick to sterile cotton swab stick and matrix surface
which is dull and sticks and curls onto sterile swab stick.
6. Depending on operative requirement surfaces should be oriented
as required.
7. Please ensure suitability of received graft for the purpose
procured. Do not use when found defective or not as per your
specifications.