= ’ FTA FS AWB Yb (AVA EY VIB) (CES REA EH OAL ff b ® 19844F PART CHE DA COR OR AT REL, Cerny 1c ko Ci AME RTE aS SNK”, AW, Deinhard Sick YB. acidoter- restris (sp.nov.) Eai% SH, 199247 Wisotzkey SILK SMMRRRIE LY Alicyclobacillus B= HSH anh. SETAESF Ha Cd % Alicyclobacillus tA RIA DAES, WENA OI OM EF CAR L, RRM ER RMOFI EMSS (A aci- doterrestris ; Dose =5.35}, EW pH25~55, EF WLEE20~55U). ERB E LCRA DUES TA FAW WEVG RREBA SESS EMMENTWS (BI). RMR AE wkd, =TYt-F-OLF GRATE PEA o TEBMERTHMLDS, ABUSE SUE R EN Ca B28, OTH MEOEETIRMBTE RVD, AMI Desh (MICpH) FRIST Sw, is 1: Fe eH 2 i I< 12 SG St — A (efi Tv B.SC HAE LE IRE E LTR SENSED, S SIME + BIC Lo CHEMOA. acidoterrestris Cd BO HADRONS. CHE TCA, acidoterrestriso 2004-2 EV ESL y DO PEROT OW Ae BBC BIURM TR BAG HR Dibohie, BMROME - REROAS DPECH 972%, PMO 1S OO MMA EEE LCE HOMO RMEOEREAALEZI AY b— Jv BAMHHO Pad 32, HI 2~ 3H SETS, MARMGIC ILA, acidoterrestrisHh 7 > AV RAVARTPCRE = 1) YES WL, Y7A TA VESRRE SE, MET HET SARE COME SNTOS, BE, Hideo MESNHMEN HS, HMC, F ORAM OV THT. Eh, KEY PEL VC BRD & ARERR TS OEY MABE ok. SURE (974 PA) ORR i a a > -4y | weak hoa | Sea ba mL On pena say xe | ypepaen DIV EASE (IESE ALA) AUER YS AGRORD peroxidase suaiacal ——S4— > Tetraguaiacol (184 x Fo, wey” Tetrnmalacal (WAAR) 1 OPP EO a1 BA COMBORK 200345 3 A, FRU Hb Se PRIS “CH RE A Oe a RERMARS NK. RETIYSCH ME WCU, 3~ S HOMIE REBTABEL, A acidoterrestris (AF AATE MES) DPI SEICILA VARY YEE REM NTS, HABER & 1L45T 18~ 20 CERO MORES TRAATE REL, HARICEST FIRB DAE ‘F (A. acidocaldarius (AF AACE RES) ETT, AATABAH) CASHETHS. CREM LT, MUAY YU TAT RAMEE LS HH D, SIEM CH 3 fH) CHETSSOMGMTHS. AUT PUT HIRI OW TISARBUC CHT B. 2 BOHR 200846 4H, MY 7 iC-CRIMMES AATEU-WG Microbiology 21th meetingl=C HAS Bt HK () ORRETORMR, WHICBYT OOHEBSRICRELOR—-AMH ENTS, SEMIS OVC AS EARLE UMN MEDC EBA TH ah Pi L TA d, BATH L YSGH OR HI EHS SOL, Eh, MP ELT BRI ESAATSER (RIN) KOVTOFY REAF KPT SOE, Wh 2 icc 200448 4 A OIFU-WG Microbiology 22th a. EMF CLARECK. 3 NVA YH— CRORE Va-YSGH EAI So EACLE D, ERMC WE (Bloppm) OF T4 FA-MEBE SE SLEMMBE GOK. Eh, LOVTAT AX MERWE YT BIREADVS SLICE TH ICRI GSE) TR JTATI-WERO MM, BRIE? = 9) VERE HN RAW OAATICG BE VacibeT 4 Va-YSGXFab, LTA T AWARE ED AALEMOETE -MDIORAETSeHH BR FPA PAA IRAETABIBSRIRIN y b Caset A= —OmH) Butter 1m F aui0, ant @Peroxidase 20.1 | RRC COPE Va-YSGH3 2 ml FABLE! 7977 LOGEMENT A AAT PAT I-N EE HTAEL ETD. 2 an=-8esm Heh (Vide: $=) > RBM) WER BM S ATECRATS. PHC OVC RY ES snk, AUAFY YEO RIS, MRE TR ROPE LYE YY —EAME ER, © NKELYITATI“WHEIYAMIT ATA SALES, CANORA LTHEDT bF YTAT AMIENS CECLS (Bi). 4 vy MOR C 4-1 Hy KICK SAATEED PTA IVE (VaYSGHh) 27 TAT I—MRWNY y bP SESE Y bABES nie (BRI). 274 7a AME, 9 FE & b UN RRR S DAATIER BAD 2AROWFHCEMIETE BL F (CH DTWS, TMAH {4 ARS 4 TRB 2 ml OVa-YSGH HE MALE LOOM 1 ey bee oThS, a0 2~ 3H CIT 4 FIVE OR MAH TR B71, TART KE LCAMCHOK, TU=—-HROF MRA we PRY IA PALA#1 me Va-VSGHEHE BLE TABIBRD IF 4 FILA eee eee eee Alicgclobaciltus genomic sp. | DSM 6 #35 & OHI 6 He Bhat P< A. acidocaldarius ‘Type strain &tsDSM 6 teas & URMER 6H | BIIZET KMS, WF NO WS MENTS SZ La 5, MERAATRIAS y bE L CHEECH D, POT ABE 722 HEAL ETFS ee (BET) BUS HRROREAE LC, 2ST OPM = — & AV T10 wl VF 1 2004-2 OppmbL:, ++; 20~30ppm, + 5 1OppmbtF Case? Siti S DAATINE RI 100,15 @Buster inl ‘OHO, 2z1 ae (OPeroxidase 20,1 —<+ || >> [aig ] i Jjaaecsome 1) YSN 97 E IN VAVSG 2a SIRaI-REE hom wR 3 AER EE GRIT) 5 DAATIEN HH DERMTSCLISS (FR). 4-2 Fy RICE SRDS OAATIORRHE RA GRO MBE YT VCH BAST 3 ~ SAR ROD Y Tov ERIE) 100014 #, Va-YSGHib 2 mitcIMd, 45 5 ~2485H 4 VHA PRL, CREAT, ABMEAR, peroxidase © MCAD L, 5 PRICBEOM ED HEENET SELVA LEBRLE 95 —Case? Sei JRF HRCI CHIR AST, SEM) LAL RRA AAT. L7>10%u/ml 10041 9 EBeaatueo t SBRRI CES ASEomT rita im aH ‘Rob th) @AAC ATCC270097, @AAC HP2, OA.genomie sp. P2, DA. genomic sp. DSM11983 BR3 An=—HeRKeL 2 Ahesperidum D7? 4 P =~ sie i [7-7] No | wR | te a | 1 | Bsuaawsor | - a A. hesperidum 4 Brg 238s (am SAE ASC, AE 3 PRE HMO AIS &T1ESDNA OMANI ISAT te HAMA M—O > OL OWT IW — THIELE, (PU TABEOBEOHV HRD Bid 2 187) 8A hesperidum 3%, EE MDS te SABO TPR YT ATI~VEEWERTSEEDAT GE Mote (B 7u—7) © A acidoterrestrist HAGE &, AMOI TAT aw ese HLL. OAMIZAATE 0 CHARDRBT B70, BACH Rowe eA EHS. (Soe mRNA) og EF WIURE LT, FLY PAR ISIO.CEU/ ml AATI# HR (TA-27) @28HL, TRIES 2ABLUS HROFY FW EXY BCH KHELEE IS, 2A AAR (CORO MILIO? CFU/ml, Lédto C¥ 7 bCUS10°CFU/BRRE Chon) Titkit (VaYSGH) 24mPRAEIC, SPSS ETH RE CIF) 5 BPP G1 BHO Ze EA ATER, Ei, SRY 7 EAP LR RF. 5 Fy hEAULREORR §-1 an=—4%e BRIKRLEC BY) AATOARV BEES Lik. Alicyclobacillus HENS OC RF L728 HUMOR LCG AL AA, MAARILAATEL SHILA, acidiphilus, A. hesperidum (2 5 #8 Ostrainlc WEIS ERT LOM tt) BE UA. herbariushtay > OF FR MIGMICILA, acidiphilus® A. hes- peridumi' ot (FRA). BLEEK OO 2 HELO TOAD RO MMA EBESAS TWEED Siw, SIT ATI MERE BMRIT ALBERS. 5— 2 AATIESE HRI ATA T Am eee CAAT) © Raa EAE A-hesperidum (Atles) 3 £ UA acidiphibus Uk, 45U, SIEBER CL (MERI | 277 4 7 1 IVA SSOppm) SH4 HRI (AHest AAP) TRIN EA BYRWi (AHes) KBUT, Fy b GRRE) Ani DUR e AHI PM EAE LZ. AAT OME 110 chu/ ARE LL © BB 1 WSR VF aN Yay CHBHETAR. EK, l0efu/ REALE OBB HITS CTH ARH EA HCH oz (HS). AHes (oa3Ve CILIA < 10%cfu/ RRL O RCH TRL EERE OBNRD A. acidorerrestris KBL304 40% 10" of /BRERE a +105 08 10 20 25 30 Av e28H 7 HID OF 7 MY OMRRMIS WILLS S COMRADE seo RT. © SSR Ou/ BURL CULE, 20a BUF CH MO TY tanya vl CMM BICC, ORM A Pay eth LPFK BAA ZAMT ETH of. BES AATENSRMRE ey Rec aAmR AR ANAS 8 DRM EME MOME® A.hesperidum KBL524 13% 10tefu/ BREE 2Abrs 05 0 15 2 Bs AV$a.8— NH) oohy heb OMMRI MAS ols LEE CORAM BWTAR. SE ACA ctu/ BURA LLC A, 10tcfu/ SR BOLT CLS ~ 2A MED 4 7 ey oY ED BLE. CMR RIM , 24 Yaya IES BRED HE Cab o Pe 6 AHos@DSEMLE A > Kame ung e FAN 3B 2004~2 TUL2AE CARMA ETH oe (HG). Litto T, AATIMOYT 4 7 3 Jvi HERS SHA CH, MSRM & 100 01H WU4 YeAN—Y a I AS IT WEA ME TH 272. 6 RADE COIP 1 PINE (Bacillus) E % OR HREMAL COA. acidoterrestrist§ BIIRIEAE & RARRETHONTHSA, PEROMATSH BWDRAREO HAMS S. MAO, TH WISB. subtilis Cd 72. (Bacillus 9 Hiz2tko FIAT A-WERROMBER (3) DOB. subtilis, B. megaterium, P. polymyza®B & UB, licheniformist= 4 7 4 7 2 — HEA MEA BoE DPA LAS. Bacillus OBE OPH ET OT I BacilusD I? 47 3— Ive He incubation a oe hrs | 72 hrs B.subtilis 6 tet [ONT 47 IFO3134 + NI. 48 IFOL3719 ee ONT, 159 - a 181 ATCO6633 | ++ Nr. aT +e | oN. | 379 . +4 382 ~ + 397 aaa NT, 4d - - B. megaterium | 155 + ONT, 156. eet | ONT. 157 + NIT. 158 leas NT, 381 oad NT. 383 B licheniformis | 50 TFO12195 B.polymyxa | 274 B.sphaericus | 303 2 - 54 - . B.coagulans | 350 JCM2257 a - Bucirewlans | 166 f = +++ i 40ppmPL, ++; 20~40ppm, +; 20ppmLUF, ~ SSRI, NT. ; not tested ~o7 —LADY hTS | Ciarens oes 438 | ———> [_158 mi BAR, YSG 450, 5 ERE Peroxidasetl Total 138 SL Cine at aA Ct | Fiz | 7 TABRtisret— Hema (Hee) WlEDS, FIAT I-VBACL REEMA FEF MIRAREDEC SOE OHS. FTA 7 A Vi BacillusOBMHE LT, N=) y#8100ppm # il LEYSGHEI (pH7), YPC HSL BOE TSBIR Me A TAS BP ~BHOt YHaN— YAEL, YTATA —VELENETS. Bou FAP Et RAE PRBS OVC UBM 7 AR Lik, HRMS Tid, FRE ARN.535. (2003 SAB) CBMSNKV, K¥y bMRIR FSNTBRUATY SERED MELE OOTHD. wR AEOMIMCE REBAR & CME RADA AR RES MAb OIE A (7 SYP W—- TER) BAUD, sb T—F YO WF BALI AREA BAD LBA LE &. Fh, RROBMRER L RIM ot MEE os AAT YSG 300, 55983 __ ——— Bonk auS=—(keTAAT ATH enets. PRO MUBASECIS b ARE TH LIRA. Sex it 1) Cerny, G, W. Hennlich, and K, Poralla, Frucht- saftverderb durch Bacilleno : Isolierung und Charakterisierung des Verderbserregers, Z. lebens-Unters, Forsch, 179, 224-227 (1984) 2) PETIIMUBR, SME PARED TPR DS) 1 TAM RE ODM LER, VIR Ry 2 HAH 2O0L5F 1B, 9.26 (2001) 3) Baumgart, J, M. Husemann, and C, Schmidt, Alicyclobacillus acidoterrestris : Vorkommen, Bedeutung und Nachweis in Getrinken und Getrinkegrundstoffen, Plissiges Obst 64, 178-180 (as97) 4) RUBE, RRERACALAT AS HLA. acidoterrestriso HMRMKO RS, RLM, LAS (2002) 5) SAMI, APY YEE Me Rib OA. acidoterrestrisH HRM EORR, RT SHMNOSA1, 9A (2003) YRNY IA RILEY R