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MDA dr.Widhi.skax
Calculation results
04/10/2022 13:00:32

Standard Curve 2
Fit type: 4PL
Concentration transformation: Logarithmic
Signal transformation: Linear
Wavelength: 450 nm
Sample group: Group 1

y = 2,14248 + ( (0,110697 - 2,14248) / ( 1 + (x/54,7156)^-0,974891) )

y = a + ( (b - a) / ( 1 + (x/c)^d) )
Where:
a= 2,14248032722783
b= 0,110697316600024
c= 54,7156278602667
d= -0,97489081318914
2

R²: 0,998

Well Sample Conc. [ng/ml] Original signal CV% Fitted signal Residual
Average Std0001 1 000 0,2418 NaN 0,2236 0,01817
A01 Std0001 1 000 0,2418 0,2236 0,01817
Average Std0002 500,0 0,2933 NaN 0,3214 -0,02807
B01 Std0002 500,0 0,2933 0,3214 -0,02807
Average Std0003 250,0 0,4755 NaN 0,4871 -0,01159
C01 Std0003 250,0 0,4755 0,4871 -0,01159
Average Std0004 125,0 0,7900 NaN 0,7382 0,05175
D01 Std0004 125,0 0,7900 0,7382 0,05175
Average Std0005 62,50 1,016 NaN 1,061 -0,04491
E01 Std0005 62,50 1,016 1,061 -0,04491
Average Std0006 31,25 1,413 NaN 1,397 0,01523
F01 Std0006 31,25 1,413 1,397 0,01523
Average Std0007 0 2,142 NaN 2,142 -0,0005803
G01 Std0007 0 2,142 2,142 -0,0005803
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MDA dr.Widhi.skax
Wavelength: 450 nm

Plate 1
Signal
1 2 3 4 5 6 7 8 9 10 11 12
A Std0001 Un0079 Un0080 Un0081 Un0082 Un0083 Un0084 Un0085 Un0086 Un0087 Un0088 Un0089

0,2418 0,1223 0,1814 0,1011 0,1466 0,07430 0,6890 0,4503 0,2304 0,1303 0,4623 0,3252

B Std0002 Un0068 Un0069 Un0070 Un0071 Un0072 Un0073 Un0074 Un0075 Un0076 Un0077 Un0078

0,2933 0,06760 0,05800 0,05780 0,08600 0,09490 0,1142 0,04650 0,05410 0,07090 0,2565 0,06080

C Std0003 Un0057 Un0058 Un0059 Un0060 Un0061 Un0062 Un0063 Un0064 Un0065 Un0066 Un0067

0,4755 0,1549 0,05510 0,1793 0,06420 0,1673 0,4643 0,1632 0,1409 0,2027 0,1796 0,1910

D Std0004 Un0046 Un0047 Un0048 Un0049 Un0050 Un0051 Un0052 Un0053 Un0054 Un0055 Un0056

0,7900 0,1086 0,3196 0,07020 0,05310 0,04310 0,7029 0,09960 0,2056 0,2115 0,1406 0,1037

E Std0005 Un0035 Un0036 Un0037 Un0038 Un0039 Un0040 Un0041 Un0042 Un0043 Un0044 Un0045

1,016 0,1594 0,1619 0,08130 0,4014 0,1980 0,2004 0,3744 0,05880 0,8154 0,07050 0,4545

F Std0006 Un0024 Un0025 Un0026 Un0027 Un0028 Un0029 Un0030 Un0031 Un0032 Un0033 Un0034

1,413 0,3157 0,2289 0,09580 0,1288 0,2561 0,1109 0,7026 0,8237 0,1921 0,05010 0,4971

G Std0007 Un0013 Un0014 Un0015 Un0016 Un0017 Un0018 Un0019 Un0020 Un0021 Un0022 Un0023

2,142 0,3151 0,6780 0,2790 0,05820 0,2872 0,5816 0,4160 0,3750 0,6681 0,5218 0,2080

H Un0001 Un0002 Un0003 Un0004 Un0005 Un0006 Un0007 Un0008 Un0009 Un0010 Un0011 Un0012

0,2923 0,05590 0,2029 0,1118 0,04570 0,05600 0,09760 0,04910 0,1290 0,3362 0,06530 0,1666

Conc. [ng/ml]
1 2 3 4 5 6 7 8 9 10 11 12
A Std0001 Un0079 Un0080 Un0081 Un0082 Un0083 Un0084 Un0085 Un0086 Un0087 Un0088 Un0089

849,8 > Max > Max > Max > Max > Max 140,8 284,2 938,7 > Max 272,2 489,8

B Std0002 Un0068 Un0069 Un0070 Un0071 Un0072 Un0073 Un0074 Un0075 Un0076 Un0077 Un0078

588,1 > Max > Max > Max > Max > Max > Max > Max > Max > Max 756,0 > Max

C Std0003 Un0057 Un0058 Un0059 Un0060 Un0061 Un0062 Un0063 Un0064 Un0065 Un0066 Un0067

260,0 > Max > Max > Max > Max > Max 270,3 > Max > Max > Max > Max > Max

D Std0004 Un0046 Un0047 Un0048 Un0049 Un0050 Un0051 Un0052 Un0053 Un0054 Un0055 Un0056

110,9 > Max 504,8 > Max > Max > Max 136,1 > Max > Max > Max > Max > Max

E Std0005 Un0035 Un0036 Un0037 Un0038 Un0039 Un0040 Un0041 Un0042 Un0043 Un0044 Un0045

68,48 > Max > Max > Max 343,2 > Max > Max 385,3 > Max 104,7 > Max 279,9

F Std0006 Un0024 Un0025 Un0026 Un0027 Un0028 Un0029 Un0030 Un0031 Un0032 Un0033 Un0034

30,23 515,8 951,6 > Max > Max 758,3 > Max 136,2 102,8 > Max > Max 241,8

G Std0007 Un0013 Un0014 Un0015 Un0016 Un0017 Un0018 Un0019 Un0020 Un0021 Un0022 Un0023

0,01267 517,6 144,7 644,5 > Max 611,1 187,0 323,5 384,3 148,4 223,5 > Max

H Un0001 Un0002 Un0003 Un0004 Un0005 Un0006 Un0007 Un0008 Un0009 Un0010 Un0011 Un0012

591,8 > Max > Max > Max > Max > Max > Max > Max > Max 462,4 > Max > Max