Autoboosting Calibre

Liquid Nitrogen Container

Operation manual
Model:
YDZ-5
YDZ-15
YDZ-30
YDZ-50
YDZ-100
Certificate of Quality YDZ-150
YDZ-175
YDZ-200
checker: YDZ-300
YDZ-500

Manufacturer: Qingdao Haier Biomedical Co., Ltd.

Address: Haier Industrial Park, Economic Technology Development
Zone, Qingdao P.R. China
Web:www.haiermedical.com
Revision Date:07/2019
Version:1st,2019

•Before using, please read the manual thoroughly.

•Our company reserves the right to the interpretation of the manual.
•For the product appearance, please make the object as the standard.
•After reading the maual, please keep it along with the invoice properly.
•Product technology or software is subject to upgrade without notice.
Content

Product Introduction ........................................................... 1

Characteristics and Functions ............................................ 4
Product Use and Others ..................................................... 5
Faults and Troubleshooting ................................................ 9
Test and Calculation of Product Daily Evaporation Rate ... 10
Product Parameters........................................................... 11

13
 Product Introduction

Model YDZ-150 YDZ-175 YDA-200 YDZ-300 YDZ-500

Overview
The series products of autoboosting calibre liquid nitrogen container manufactured by Haier are
Geometric volume L 165 185 220 330 550 widely used in sectors of animal husbandry, medical care, scientific research and industries, providing
good loading tools for storage, transport and supplementary of liquid nitrogen.
Effective volume L 150 175 200 300 500
Haier is a specialized enterprise in manufacturing autoboosting calibre liquid nitrogen container.
External diameter mm 706 706 758 857 1008 The production of products is in strict accordance with GB 16774-1997 Autoboosting Calibre Liquid
Nitrogen Container.
Height mm 1188 1298 1265 1459 1576
Methods for Compiling Product Models
Bare weight kg 102 120 130 202 255
Extracted from GB 16774-1997 Autoboosting Calibre Liquid Nitrogen Container:
Calibre mm 40 40 40 40 40 Y D Z - □ W

Daily evaporation
rate of % 1.3 1.2 1.2 1.1 1.1
static liquid nitrogen
The first letter of Chinese pinyin “Woshi
(horizontal)”, no note for the vertical
Standard working
MPA 0.05 0.05 0.05 0.05 0.05
pressure Effective volume of container (L)
Maximum working The first letter of Chinese pinyin “Zi
MPA 0.09 0.09 0.09 0.09 0.09
pressure (Autoboosting)”

Volume of liquid The first letter of Chinese pinyin “Dan

L/min ≥6 ≥6 ≥8 ≥8 ≥10 (nitrogen)”
transfusion
The first letter of Chinese pinyin “Ye
Opening pressure
(liquid)”
of MPA 0.099 0.099 0.099 0.099 0.099
primary safety valve Example 1: YDZ-5 indicates the vertical autoboosting calibre liquid nitrogen container
Opening pressure with the effective volume of 5 L.
of Example 2: YDZ-200W indicates the horizontal autoboosting calibre liquid nitrogen
MPA 0.15 0.15 0.15 0.15 0.15
secondary safety
container with the effective volume of 200 L.
valve

Opening pressure
Product Structure Diagram and Principle of Autoboosting
MPA - - 0.47 0.47 0.47
of rupture disk
1.Product Structure
Indicating range of The container consists of enclosure, inner tank, transport trolley, drainpipe, valves of all types,
MPA 0~0.25 0~0.25 0~0.25 0~0.25 0~0.25
pressure gage pressure gauge, vacuum seal joint, etc. (see Fig. 1 and Fig. 2):

12 1
 Product Parameters

Plug screw Model YDZ-5 YDZ-15 YDZ-30 YDZ-50 YDZ-100

Geometric volume L 5.5 16.5 32 55 110

Valve Effective volume L 5 15 30 50 100

Valve cup External diameter mm 329 404 454 506 606

Height mm 510 750 879 991 1185

Neck tube

Outer upper Bare weight kg 15 23 32 54 75

end plate
Drain pipe Calibre mm 40 40 40 40 40
Inner tank
Daily evaporation rate of
% 3 2.5 2.5 2 1.3
static liquid nitrogen
Ascending pipe
Standard working
MPA 0.05 0.05 0.05 0.05 0.05
Outer lower end plate
pressure

Foot ring Maximum working

MPA 0.09 0.09 0.09 0.09 0.09
pressure
Fig. 1 Product Structure Diagram
Volume of liquid
L/min ≥2 ≥2 ≥3 ≥3 ≥4
transfusion

Opening pressure of
MPA 0.099 0.099 0.099 0.099 0.099
primary safety valve

Opening pressure of
MPA 0.15 0.15 0.15 0.15 0.15
secondary safety valve

Opening pressure of
MPA - - - - -
Exhaust valve Drain valve rupture disk

Indicating range of
MPA 0~0.25 0~0.25 0~0.25 0~0.25 0~0.25
pressure gage

Safety valve
Pressure gauge
Pressure
increasing
valve

Fig. 2 Valve Diagram

2 11
Test and Calculation of Product Daily Evaporation Rate
1. Adopt weighing method for measuring daily evaporation rate of the product. 2. Principle of autoboosting:
2. The test shall be conducted with ambient temperature as 20℃ ±3℃ and under ordinary pressure. When liquid nitrogen is loaded in the inner tank, exhaust valve, drain valve and pressure increasing
Meanwhile, the test shall not be conducted in sites with direct ventilation. valve are closed and the plug screw at the filler box for the liquid nitrogen is tightened.

3. The test shall be conducted with fullness rate of liquid nitrogen in the container no less than 50% In case of no leakage for the above, part of the liquid nitrogen entering into the pipe will be gasified
and static precooling duration as 48 h. The test lasts for 3 d. through heat absorption due to the heat transmission made by the enclosure to the ascending pipe.
When opening the pressure increasing valve, the gasified nitrogen will immediately enter into the
4. Maximum capacity of the weighing apparatus used by weighing method shall be no more than upper space of the liquid level in the inner tank through valve; meanwhile, the liquid nitrogen in the
4 times higher than total mass of the product when filled with liquid nitrogen, the accuracy shall be inner tank of the container will be gasified through heat absorption as they continuously enter into the
superior or equal to 1/3000. ascending pipe. Since the volume of gasified nitrogen is over 600 times than that of liquid nitrogen,
large amounts of nitrogen gas obtained by a small volume of liquid nitrogen will continuously enter
5. Daily evaporation rate of the product α is calculated as follows: into the inner tank through the opened pressure increasing valve. As the amount of entering nitrogen
gas increases, the nitrogen gas aggregated at the upper space of the liquid level in the inner tank
begins to generate pressure on the inner tank wall and the liquid level gradually. When the value
- of the pressure gauge reaches 0.02 MPa, open the drain valve, the liquid nitrogen will be drained
through the drainpipe.
Wherein:
α— daily evaporation rate, %;
qm—Average daily evaporation capacity calculated based on total mass of liquid nitrogen lost in 3 d,
kg;
m—total mass of the product after injected with liquid, kg;
me — bare weight of the product, kg.
Note: Test and calculation of daily evaporation rate is in accordance with GB16774-1997. If the test
is not conducted with specified ambient temperature and under specified atmospheric pressure,
or minimum division value of the measuring instrument varies from standard requirements, the
calculated value and actual evaporation rate of the product may differ.

10 3
Characteristics and Functions Faults and Troubleshooting
1.Components and Parts
Key characteristics of products are as follows: Handles of pressure
There are moisture
• Convenient for filling liquid nitrogen: just screw off the plug screw at the filler box for the liquid increasing valve, exhaust
1 or ice particles in the
nitrogen, the liquid nitrogen can be filled by the drain outlet or the pipeline of fractionator of liquid valve, drain valve are too
valve.
nitrogen machine. tight to operate
• The pressurization method that the container exerts pressure to the inner tank by gasification of a
small volume of liquid nitrogen in it is easy to operate with instant outcome; meanwhile, the purity of
liquid nitrogen is guaranteed. Air leakage in connection
parts of drain valve,
• The high intensity of container makes it applicable to short distance transport. 2
exhaust valve and valve
• The draining pressure of container is extremely low and the container is mounted with pressure seat.
gauge, safety valve and vacuum seal joint. Therefore, the safety and reliability for use of container
are high.
Though the pressure
• Enclosure, inner tank and main components and parts are all manufactured by austenitic stainless
increasing valve is
steel, for which the vacuum and low temperature performance are good.
3 closed, pressure in the
2.Primary functions of each components and parts are as follows: container increases
slowly.
• Inner tank: used for loading liquid nitrogen.
• Plug screw: seal at the filler box for the liquid nitrogen, connected by screw thread and equipped
Long period or
with low temperature resistant PTFE seal ring as to prevent leakage of nitrogen gas, which resulted
4 impossibility of pressure
in pressure reduction.
increasing
• Exhaust valve: deflate and reduce pressure as per need. When screwing off the plug screw at the
filler box for the liquid nitrogen, the exhaust valve must be opened before deflation and pressure Though there is internal
relief. The valve shall be opened when the container is not in use. pressure, it drains slowly
5 Melt ice and remove water.
or the liquid can not be
• Pressure increasing valve: when it is opened, the pressure in inner tank will rise. It shall be tightly
drained.
closed when it is not in use.
• Pressure gauge: used for indicating the pressure magnitude of inner tank. Internal pressure of the
container has reached
• Safety valve: control the pressure of inner tank under the maximum working pressure; once 6 more than 0.09MPa, the
the pressure of inner tank exceeds the maximum working pressure, the safety valve will open primary safety valve still
automatically to deflate and release pressure. does not open.
• Vacuum seal joint: the aspirating hole of the vacuum space in the manufacturing of container. It
has the effect of vacuum pumping; and once the leakage of inner tank generates inner pressure in
After the safety valve
the vacuum space, it has the effect of pressure relief protection of inner tank and enclosure.
is opened, internal
pressure of the container
decreases to less than
7 surface of the safety
the setting pressure, the
spring can not restore
and the valve continue
jetting gas.
4 9
If it happens during the process of using,
heat the valve with blow dryer as a
temporary measure, and then open the
valve to remove moisture or ice particles
after liquid nitrogen in the container is
evacuated completely.
There is air leakage
in connection parts of
valve rod and valve Replace PTFE seal rings or screwed
body. The washer or sealing materials.
sealing materials are
damaged.
There is air leakage in
1. Reclose the valve;
the pressure increasing
2.. Replace PTFE seal rings or screwed
valve, or the valve is
sealing materials.
not closed tightly.
1.The ascending pipe
1. Melt ice and remove water;
or pressure increasing
2.Add water with soap, check questionable
valve freezes;
part of air leakage and remove leakage.
2.Air leakage.
1.The drainpipe or
drain valve freezes;
2. No liquid nitrogen in
the container.
1. The safety valve 1. Melt ice and remove water;
freezes; 2. Clean up foreign matters on the sealing
2. Setting pressure surface;
of the safety valve is 3. Send to the manufacturer to readjust
shifted. the setting pressure.
1. The safety valve
freezes;
2.There are foreign 1.Melt ice and remove water;
matters on the sealing 2.Clean up foreign matters on the sealing
surface;
valve; 3. Send to the manufacturer to readjust
3. Setting pressure the setting pressure.
of the safety valve is
shifted.

a) Exclude the residual liquid nitrogen in the container with pneumatic injection. The method is:
tighten the plug screw at the filler box for the liquid nitrogen, close the pressure increasing valve and
open the drain valve. Pressurize from the exhaust valve to the container with nitrogen gas or other
dry gas (it is forbidden to use oxygen or other combustible gas) until the residual liquid nitrogen in
the container is completely drained through the drain valve. If block in drain valve is found, remove
the drain valve and observe to check if there are ice particles blocking the corner of the valve seat
screw hole and the drain valve body. If there is any block, remove the ice particles with hard steel
wire or melt ice with blow dryer or hot blast drying machine (temperature is not larger than 40℃ )
before drainage.
b) In case of completely draining the residual liquid nitrogen in the container, close the drain valve
and remove the exterior pipe of the pressure increasing valve (dotted portion in Fig. 5). Continuously
inject hot wind with pressure of roughly 0.05 MPa under normal temperature or temperature not
larger than 40 ℃ from the drain valve for several hours. When no water drops gas ejected from A
increases gradually, it indicates that the ascending pipe is unblocked and the ice melting and water
removing work is done.
Note: due to different humidity in various places, use frequencies of containers and injection
methods of liquid nitrogen, the interval time of ice melting measures shall be taken by users
appropriately.
•Measuring Method of Liquid Nitrogen Volume in Inner Tank
a) Estimation Method with Deeper Scale. When used, insert the deeper scale into the container
until the deeper scale contacts with the bottom of the inner tank. Stand for 15 seconds before take it
out. The deeper scale is exposed to the air. Since the soaking part of the deeper scale in the liquid
nitrogen will form a layer of white frost when exposed to the air, the reserve of liquid nitrogen may
be roughly estimated through the dividing line of the deeper scale.
b) Weighing Method. Measure the quality of container loading liquid nitrogen with weighing
apparatus and deduct the empty weight of the container set in Table 1 of this Manual. The quality of
liquid nitrogen in the container will be calculated. And then the volume (density of liquid nitrogen is
about 0.825 kg/L) of liquid nitrogen will be correspondingly calculated. The precision of this method
is higher than that of estimation method with deeper scale.
8 5
Product Use and Others
1.Product Use Instructions
• Before the use of product, user shall unpack and inspect the product appearance quality and
check whether the accessories are complete or not. If the appearance is found to be defective
or the accessories are not complete, please send the information back to our company within 5
workdays upon receiving the product. Retailers and dealers shall perform in accordance with the
relevant terms speculated in the retailing and dealing agreements.
• The product is a high vacuum low temperature container and we suggest user assigning specially
assigned person for operation. Furthermore, this manual shall be read carefully and operation shall
be done in accordance with this manual.
• Since the temperature of liquid nitrogen is extremely low (-195.8 ), please put on the leather
glove during operation. Do not wear boots as to prevent cold injury to foot by liquid nitrogen falling
into the boots.
• Direct sunlight shall be avoided for the container and the container shall be stored in shady and
ventilated place. During operation, open the doors and windows of the room for ventilation; avoid
suffocation due to indoor hypoxia.
• The vacuum seal joint had been sealed well before the product left the factory and had been
covered with the vacuum seal joint cap for protection. User is strictly prohibited to remove the
vacuum seal joint cap.
• The container is mounted with safety valve. The setting pressure of the safety valve had been
adjusted well before the product left the factory. The safety valve was lead sealed and user must
not remove it. Our company refuses the warranty once it is removed.
• In order to ensure that the pressure gauge indicates the correct and accurate pressure magnitude,
take periodical examination on its precision; meanwhile, please avoid colliding the dial plate to
prevent distortion resulting in error of indication.
• Pressure increasing valve and exhaust valve can control the pressure magnitude inside the
container while drain valve can control the draining flow of liquid nitrogen. When used, don’t overly
tighten the handle to avoid damaging the valve; meanwhile, please note the rotating direction of the
valve handle: counterclockwise is on while clockwise is off. Be sure to close the pressure increasing
valve and open the exhaust valve when the container is not in use.
• When the pressure gauge indicates that there is inner pressure in inner tank, the plug screw at
the filler box for the liquid nitrogen must not be screwed off. The exhaust valve shall be opened for
pressure relief of inner tank at first.
• The plug screw at the filler box for the liquid nitrogen is the sealing part for pressure increasing.
After drainage, the screw thread is easy to be frozen and is difficult to twist. In such a case,
unfreeze it by natural re-warming or warming up through a blow dryer before remove it.
• Foreign matters are not allowed to enter into the plug screw. For missing of plug screw, please
contact our company for purchasing. It cannot be replaced by other parts.
• For the use of metal hose for drainage, if the bending radius is too small, it will be damaged.
• Forbid welding on the container surface.
• Since the product involves several professional skills, it must be sent back to our company for
inspection and repair in case of failure.
3.Operation of Product • Container Handling
• Injection of Liquid Nitrogen a) Indoor movement. The container is equipped with 4 high intensity casters and can be moved
Firstly, open the exhaust valve and close the pressure increasing valve and the drain valve to indoor lightly and easily. As the product is high vacuum, move it as slowly as possible to avoid shock
make the value of pressure gauge decrease to zero; then remove the plug screw at the filler box or collision; otherwise, the enclosure may be sunken and performance may degrade, resulting in
for the liquid nitrogen with a wrench. Inject liquid nitrogen into the container by pipe; or inject liquid internal structure damage. Therefore, pay special attention to be careful when moving container.
nitrogen into the container with a liquid nitrogen machine. Note: when injecting liquid nitrogen into b) Hoisting. The product is high vacuum container. Before hoisting, harness the steel wire rope to
the container, for safety, don’t inject completely and a certain gas phase space must be retained for the ring; when hoisting, must not pull violently and lay down heavily and aslope hoisting.
roughly 10% of the geometric volume of the container. See Table 1 for the On/Off status of each valve during operation of product:
• Pressure Increasing Table 1 On/Off status of Valves
When draining liquid nitrogen, the pressure in the inner tank needs to be increased. When
increasing pressure, exhaust valve and drain valve shall be closed. Open the pressure increasing Name of Valve
valve and observe that the pressure indication value will increase gradually. Operation
Drain Valve Exhaust Valve Pressure Increasing Valve
The maximum working pressure gauge value is 0.09 MPa; however, in order to reduce the loss of
liquid nitrogen as possible, it is enough to increase to 0.05 MPa and this is relatively economic. Injection of liquid nitrogen Off On Off

When pressure increases to 0.02 MPa, it can drain. It is not necessary to open the pressure Pressuring increasing Off Off On
increasing valve if only a small amount of liquid nitrogen is needed; open the pressure increasing
Drainage On Off On
valve for continuous drainage.
After opening the pressure increasing valve, several places at the bottom of the enclosure may Pressure relief Off On Off
be frosted. It is not a failure but a normal phenomenon of container operation. When the container Static storage of container Off On Off
is completely filled with liquid nitrogen (gas phase space of 10% of the geometric volume of the
container is still retained), the required time for pressure increasing to 0.05 MPa is about 20 Medium long distance transport Off On Off
seconds; when the reserve of liquid nitrogen is 3/4, the required time for pressure increasing to 0.05
MPa is about 1 minute; when the reserve of liquid nitrogen is 1/4, the required time for pressure No storage of liquid nitrogen Off Off Off
increasing to 0.05 MPa is about 8 minutes. As the liquid nitrogen in the container decreases, the
4. Others
required time for pressure increasing will increase.
•Technical Operation of Ice Melting and Unfreezing
• Drainage
During the long-term use process, the inner tank may accumulate damp air gradually and the
Screw the metal hose (accompanying accessory) in the drain valve joint, close the exhaust valve
moisture in the damp air may form micro ice particles under low temperature. Such micro ice
and open the pressure increasing valve. When the pressure of inner tank increases to 0.05 MPa as
particles attach to the pipe wall and block the ascending pipe and the drainpipe. Following adverse
indicated in the pressure gauge, open the drain valve and the liquid nitrogen will be drained; when
consequences may occur if no corresponding ice melting measures to be taken at this time:
the pressure gauge value indicates 0.05 MPa, drainage is relatively economic; if drain when the
pressure gauge of the inner tank indicates a value larger than 0.05 MPa, the drainage volume of a) Pressure increases very slowly or doesn’t increase at all;
liquid nitrogen will correspondingly increase. b) In spite of the inner pressure, drainage is slow or no liquid nitrogen is drained at all. In order to
• Pressure Relief prolong the interval time of ice melting, following matters must be noted:
After end of drainage of liquid nitrogen, the pressure increasing valve and the drain valve shall be a.Shorten the liquid injection time as possible during the liquid injection process to reduce the
closed; then open the exhaust valve to release the pressure inside the container. accumulated volume of damp air into the container;
• Static Storage of Container b.In case of no storage of liquid in the container, all the valves must be closed to eliminate damp
air into the container.
In order to avoid damp air entering into the container, forming frozen particles and blocking the
pressure increasing pipe, the plug screw at the filler box for the liquid nitrogen, pressure increasing Once the ascending pipe is blocked by ice particles and cannot increase pressure, following ice
valve and drain valve can be closed and open the exhaust valve. melting measures can be taken:

6 7