ASJA Girls’ College, San Fernando

Form 5 CSEC BIOLOGY

List of Laboratory Exercises

Lab
Syllabus SBA Skill(s)
No Lab Exercise Topic
Objective assessed
.
1. To investigate the external features of a dicotyledonous leaf.
To investigate the effect of different Practice
1. PHOTOSYNTHESIS SO 2.3
solutions on potato tissue. ORR
TITLE: OSMOSIS
To investigate the external features of a Practice
SKILL:
2. OBSERVATION, RECORDING, REPORTING (NON-SBA) OSMOSIS SO 1.6
dicotyledonous leaf. D
EXPERIMENT: TO INVESTIGATE THE EFFECT OF DIFFERENT
To investigate the effect of osmosis in onion
3. SOLUTIONS ON POTATO TISSUE. OSMOSIS SO 1.6 M/M
tissue.
To investigate whether chlorophyll is Practice
4.
Criteria Mark Mark PHOTOSYNTHESIS
Awarded SO 2.2
needed for photosynthesis. ORR
Reporting:
To perform confirmatory food tests using Practice
5. clearly stated in CAPITAL LETTERS
Aim 1 FOOD TEST SO 2.6
prepared samples. ORR
Use of Language 1
To investigate effect of hydrogen peroxide
Format of Report 1 Practice
6. on raw and boiled potato and christophene ENZYME ACTION SO 2.9
Logical Method 1 A/I
tissue.
Recording:
To investigate the effect of temperature on
Methodology concise 1 ENZYME ACTION A/I
7. the enzyme amylase and its breakdown of SO 2.9
Table neatly enclosed (layout) 1
starch.
Table title in CAPITAL LETTERS placed 1
To investigate the external features of a fish
above
8. the Table and underlined RESPIRATION SO 3.4 DR
gill.
Observations:
To investigate the rate of water retention TRANSPORT IN
 9. Texture changes accurately 3 SO 4.7 ORR
and uptake in a leafy shoot PLANTS
observed
To identify simulated urine samples by SO 5.4 Practice
10.Textures for cylinders recorded for NUTRITION/DISEASE
observations and testing A/I
two samples (2 marks)
To investigate the response of earthworms Practice
11.Less than 2 textures of cylinders SENSITIVITY SO 7.2
to light and moisture. ORR
recorded (1 mark)
To investigate the germination of seeds (red
12.No textures recorded (0 marks) GROWTH SO 8.3 ORR
bean).
TOTAL 10
To draw a longitudinal section of a hibiscus REPRODUCTION IN Practice
13. SO 9.7
flower. PLANTS D
Practice
14. To investigate variations in heights. GENETICS SO 3.3
ORR
Plan and design an experiment to Practice
15. TRANSPIRATION SO 4.7
investigate if light affects transpiration rates P/D
To investigate the effects of temperature on
16. ENZYMES SO 2.9 P/D
the rate of spoilage of fresh cow's milk
To determine what type of soil will be
Draft
17. suitable for a rain garden. (INVESTIGATIVE SOIL SO 2.3
P/D
REPORT)
To determine what type of soil will be
18. suitable for a rain garden (INVESTIGATIVE SOIL SO 2.3 P/D
REPORT)
To investigate the distribution of species SO 2.1
19. ECOLOGY MM
using the quadrat method of sampling
To determine what type of soil will be
20. suitable for a rain garden (INVESTIGATIVE SOIL SO 2.3 A/I
REPORT)
2. To investigate the external features of a dicotyledonous leaf.

TITLE: PHOTOSYNTHESIS
SKILL: DRAWING (NON-SBA)
EXPERIMENT: TO DRAW THE EXTERNAL STRUCTURE OF A LEAF.

Criteria Mark Mark Awarded

Accuracy of representation 1
Correct labels 1
Title 1
Labels lines pointing to structures with no arrowheads 1
View 1
Clear continuous lines 1
No shading 1
Neatness 1
Proportional parts 1
Correct magnification total 1
TOTAL 10

3. To investigate the effect of osmosis in onion tissue.

TITLE: OSMOSIS
SKILL: MEASUREMENT AND MANIPULATION (SBA)
EXPERIMENT: TO INVESTIGATE OSMOSIS IN ONION TISSUE.

Criteria Mark Mark Awarded

Onion rings almost the same diameter 2
Each ring has the same thickness 2
Each ring completely immersed in solution 2
Original diameter of rings accurately measured (rings 1 and 2) 2
Final diameter of ring accurately measured (rings 1 and 2) 2
4. To investigate whether chlorophyll is needed for photosynthesis.

TITLE: PHOTOSYNTHESIS
SKILL: OBSERVATION, RECORDING, REPORTING (NON-SBA)
EXPERIMENT: TO TEST A LEAF FOR STARCH AND TO INVESTIGATE IF
CHLOROPHYLL IS NECESSARY FOR PHOTOSYNTHESIS.

Criteria Mark Mark Awarded

Aim clearly stated 1
List all apparatus and materials 1
Method with all steps accurately 1
described
Method in point form 1
Method in past tense 1
Results: Drawing of leaf before 1
treatment
Results: Drawing of leaf after treatment 1
Accurate key used for diagram 1
Conclusion 1
Follow the correct lab format 1
TOTAL 10
5. To perform confirmatory food tests using prepared samples.

TITLE: FOOD TEST

SKILL: OBSERVATION, RECORDING, REPORTING (NON-SBA)
EXPERIMENT: TO PERFORM CONFIRMATORY FOOD TESTS USING PREPARED SAMPLES

Criteria Mark Mark Awarded

Aim clearly stated in capital letters 1
Proper format of lab report 1
All methods for each food test listed in logical order in table 1
Appropriate title in capitals stated above table underlined 1
Method recorded in past tense reported speech 1
Observation and Record of:
 the colour change of the sample after the non- reducing sugar test 1
 the colour change of the sample after the starch test 1
 the colour change of the sample after the protein test 1
 the appearance of the sample after the lipid test 1
 the colour change of the sample after the reducing sugar test 1
TOTAL 10

6. To investigate effect of hydrogen peroxide on raw and boiled potato and christophene tissue.

TITLE: ENZYME ACTION

SKILL: ANALYSIS AND INTERPRETATION (NON-SBA)
AIM: TO INVESTIGATE THE EFFECT OF HYDROGEN PEROIXDE ON POTATO AND CHRISTOPHENE.

Criteria Mar Mark Awarded

k
Background information
1. Definition of an enzyme 1
7. To investigate the effect of temperature on the enzyme amylase and its breakdown of starch.

TITLE: ENZYME ACTION

SKILL: ANALYSIS AND INTERPRETATION (SBA)
AIM: TO INVESTIGATE THE EFFECT OF TEMPERATURE ON THE RATE OF ENZYME ACTION.

Criteria Mark Mark Awarded

Background information
1. Definition of an enzyme 1
2. Reaction of amylase on starch 1
Explanation of observations - 15 °C
Little enzyme activity, 1
temperature too low 1
Explanation of observations - Room Temperature
Some enzyme activity; Breakdown of starch 1
Temperature closer to optimum 1
Explanation of observations - 50 °C
Justify the observation on
Relation to temperature 1
Infer maximum activity 1
Limitations
Difficulty in maintaining temperatures 1
Conclusion (any 1)
 As temperatures increases the rate of reaction increases
1
 Identify the optimum temperature form the three (3)
temperatures
TOTAL 10
 8. To investigate the external features of a fish gill.

TITLE: RESPIRATION
SKILL: DRAWING (SBA)
EXPERIMENT: TO INVESTIGATE THE EXTERNAL FEATURES OF A FISH GILL.

Criteria Mark Mark Awarded

Faithfulness of reproduction ( looks like specimen ) 1
Reasonable proportions 1
Correct magnification calculated 1
Neat Labels lines pointing to structures with no arrowheads 1
Title in bold capitals ,underlined 1
Accurate labels with annotations 1
Label lines justified 1
Clean continuous lines 1
No shading or unnecessary details 1
Reasonable size of drawing (more than ½ page) 1
TOTAL 10

9. To investigate the rate of water retention and uptake in a leafy shoot

TITLE: TRANSPORT IN PLANTS

SKILL: OBSERVATION, RECORDING, REPORTING (SBA)
AIM: TO INVESTIGATE THE RATE OF WATER UPTAKE AND RETENTION IN A LEAFY SHOOT.

Criteria Mark Mark Awarded

 10. To identify simulated urine samples by observations and testing

TITLE: NUTRITION/DISEASE
SKILL: Analysis and Interpretation (NON-SBA)
EXPERIMENT: TO IDENTIFY SIMULATED URINE SAMPLES BY OBSERVATION AND TESTING.

Criteria Mark Mark Awarded

Identity of simulated urine samples:
Healthy person – G 1
Diabetic person – H 1
Worker in dry factory – E 1
Dilute urine – D 1
Protein in urine – F 1
Discussion should include justifying identity
 G – no protein, no glucose in the urine 1
 H – glucose in the urine, glucose concentration 1
higher than in the blood, deficiency of insulin
 E – darker urine, sweating occurred, ADH 1
secreted, large quantities of urine produced
 D – lighted coloured urine, less ADH secreted, 1
large quantities of urine produced
 F – Tested positively for proteins, due to 1
rupturing of the glomerulus
TOTAL 10

11. To investigate the response of earthworms to light and moisture.

TITLE: SENSITIVITY
SKILL: OBSERVATION, RECORDING AND REPORTING (NON-SBA)
EXPERIMENT: TO INVESTIGATE THE RESPONSES OF INVERTEBRATES TO DIFFERENT STIMULI.

Criteria Mark Mark Awarded

 12. To investigate the germination of seeds (red bean).

TITLE: GROWTH AND DEVELOPMENT

SKILL: OBSERVATION, RECORDING AND REPORTING (SBA)
EXPERIMENT: TO OBSERVE AND DRAW THE GERMINATION OF A DICOTYLEDONOUS PLANT.

Criteria Mark Mark Awarded

Reporting:
Aim clearly stated in capital letters 1
Proper format of lab report 1
Use of Language 1
Reflection related to everyday life 1
Recording:
Methodology concise and logically stated 1
Appropriate table showing length of shoot over time period 1
Title in CAPITAL LETTERS, placed below ALL drawings, underlined
1
Observation: Accurate Annotations
-Indication of growth of radicle first 1
-Proper annotations of plumule and radicle growing into shoot and 1
root respectively
-Indication of cotyledon deteriorating over time as seedling grows 1
with correct reason
TOTAL 10

13. To draw a longitudinal section of a hibiscus flower.

TITLE: REPRODUCTION
SKILL: DRAWING (NON-SBA)
EXPERIMENT: TO OBSERVE AND DRAW A LONGITUDINAL SECTION OF A HIBISCUS FLOWER.

Criteria Mark Mark Awarded

 14. To investigate variations in heights.

TITLE: GENETICS AND VARIATION

SKILL: OBSERVATION, RECORDING AND REPORTING (NON-SBA)
EXPERIMENT: TO INVESTIGATE THE CONTINUOUS VARIATION ON HEIGHT.

Criteria Mark Mark Awarded

Aim clearly stated in capital letters 1
Correct format of lab in proper sequence 1
Method in past tense 1
Appropriate table with correct columns 1
Title placed above table , underlined, in capital letters 1
Table neatly enclosed 1
Correct axes on histogram ( dependent variable in y axis) 1
Descriptive title of histogram 1
Title of histogram in capital letters at base of graph 1
More than half of page used to make histogram. 1
TOTAL 10

15. Plan and design an experiment to investigate if light affects transpiration rates

TITLE: TRANSPORT
SKILL: PLANNING AND DESIGN (NON-SBA)
EXPERIMENT: TO INVESTIGATE IF LIGHT AFFECTS TRANSPIRATION RATES.
16. To investigate the effects of temperature on the rate of spoilage of fresh cow's milk

TITLE: ENZYMES
SKILL: PLANNING AND DESIGN (SBA)
EXPERIMENT: TO INVESTIGATE THE EFFECT OF TEMPERATURE ON THE RATE OF
SPOILAGE OF COWS MILK.

Criteria Mark Mark Awarded

Hypothesis stated 1
Hypothesis testable 1
Aim testing hypothesis 1
Apparatus and Materials 1
Method – logical sequence of all steps 1
in present tense 1
Constant variables stated 1
Responding variable/observations to be made stated 1
Expected Results and Interpretation 1
Limitations stated 1
TOTAL 10

17. To determine what type of soil will be suitable for a rain garden. (INVESTIGATIVE REPORT) draft
 TITLE: SOILS
SKILL: PLANNING AND DESIGN (DRAFT)
EXPERIMENT: TO INVESTIGATE WHICH SOIL IS MORE APPROPRIATE TO USE IN A RAIN GARDEN.

Criteria Mark Mark Awarded

Hypothesis with one variable and testable 1
Aim related to hypothesis 1
All apparatus and materials listed in (i) detail and 1
(ii) appropriate 1
Method with logical sequence of steps 1
Method in command and future tense 1
Variables held constant listed
(i)volume of water used 1
(ii) mass of soil used 1
Manipulating variable stated 1
Expected results in table (i) reasonable and 1
(ii) linked to method) 1
Limitations stated 1
TOTAL 12
TOTAL 10

18. To determine what type of soil will be suitable for a rain garden (INVESTIGATIVE REPORT)
TITLE: SOILS
SKILL: PLANNING AND DESIGN
EXPERIMENT: TO INVESTIGATE WHICH SOIL IS MORE APPROPRIATE TO USE IN A RAIN GARDEN.

Criteria Mark Mark Awarded

Hypothesis with one variable and testable 1
Aim related to hypothesis 1
All apparatus and materials listed in (i) detail and 1
 19. To investigate the distribution of species using the quadrat method of sampling
TITLE: ECOLOGY
SKILL: MEASUREMENT AND MANIPULATION (SBA)
EXPERIMENT: TO INVESTIGATE THE DISTRIBUTION OF SPECIES BY RANDOM SAMPLING.

Criteria Mark Mark Awarded

Construction of quadrat 1
Correct dimensions of quadrat 1
Appropriate material used to build quadrat 1
(wood, wire or metal rods)
Proper use of quadrat: Random throws within 1
chosen area
Correct identification of different species 1
Detailed and consistent collection of data 1
Accurate counts of each species 1
Correct calculation of
 Species density 1
 Percent species frequency 1
1
 Percent species cover
TOTAL 10
20. To determine what type of soil will be suitable for a rain garden (INVESTIGATIVE REPORT)

TITLE: ECOLOGY
SKILL: ANALYSIS AND INTERPRETATION (AI)
EXPERIMENT: TO INVESTIGATE WHICH SOIL IS MORE APPROPRIATE TO USE IN A RAIN GARDEN.

Criteria Mark Mark Awarded

Method (i) relevant to research problem 1
(ii) in past tense 1
Discussion
 Relevant background knowledge about the TWO soil types 2
used in experiment (Refer to particle sizes and air space,
drainage capacity or water retention capacity).
 Comparison with actual results and expected results. 1
 Making accurate decision on which soil type would be better 1
for a rain garden based on results
 Suggestions of methods or modifications to improve 1
experiment
At least one Limitation stated 1
At least one Precautions stated 1
Reflections expressed (relevance of experiment to real life,
1
knowledge gained from experiment)
Conclusion (i) Stated 1
(ii) Related to aim 1
TOTAL 12
TOTAL 10