St.

Scholastica’s College Tacloban

Maharlika Highway, Brgy. Campetic, Palo Leyte
DEPARTMENT OF MEDICAL LABORATORY SCIENCE
S.Y. 2021 – 2022

Laboratory Manual
in
MLS Course Code No. 8

“If you cannot appreciate how plain and awesome

Prepared by: our daily life is, you are just living foolishly”

Mr. Vic Deniel A. Busi, RMT -Chain User

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BATCH NAME

Name of Student: _____________________________ Group Name: ________________

Year and Section: _____________________________ Contact Number: _____________

 St. Scholastica’s College Tacloban
Maharlika Highway, Brgy. Campetic, Palo Leyte
DEPARTMENT OF MEDICAL LABORATORY SCIENCE
S.Y. 2021 – 2022

Policies/Guidelines inside the Laboratory

1. ATTIRE. Laboratory gowns should always be worn by students inside the laboratory. Nobody is
allowed to perform the exercise without a laboratory gown. “No gown, no lab and no lab mean a
grade of 5 in that particular exercise”.

2. FOOD. Eating inside the laboratory is strictly prohibited even if one is not performing a procedure.

3. TABLETOP DISINFECTION. Disinfect working tables before and after performing a laboratory
procedure to avoid contamination.

4. HANDWASHING. Wash your hands before and after working in the lab with detergent and dry
them with the use of paper towel.

5. Always read the procedure before starting and read instructions carefully. When in doubt, do
not hesitate to ask the help of the laboratory instructor.

6. Always handle all equipment and glasswares with utmost care.

7. Remember that all specimens used in the exercises are potentially infectious, therefore never play
nor tinker with them unnecessarily. Instead, handle these specimens with utmost care.

8. PIPETTING. Transferring solutions or cultures by pipette must always be performed with a

mechanical suction device. Note: Mouth Pipetting must not be done.

9. Always dispose waste materials (applicator sticks, cotton swabs, cotton balls, tissue papers) or any
other objects in their proper containers. Never leave them on the tables or chairs nor throw them on the
floor.

10. DISPOSAL OF USED CULTURE. All specimens, cultures, and other materials that have been
examined in a microbiological laboratory must be made noninfectious before discarding. This is
necessary to protect all those in the laboratory or who may handle or come in contact with the waste
material before its final disposal. You will only be growing bacteria and yeast for Microscopic
analysis of the urine.

11. Keep the laboratory clean by picking up pieces of papers, wrappers, etc. and throw them inside the
waste can. Never leave the room dirty.

12. ACCIDENTAL SPILLS. All accidental spills, whether chemical or biological, notify the
laboratory instructor immediately.

13. If you are injured (burned or cut) notify your instructor immediately.

14. Students who are caught wearing their lab coats outside the laboratory during the period will be
deferred immediately to continue the activity. At the same time, they will receive a failing grade of
5.0 for committing the violation.

15. Students who are caught taking selfies and other nonessential photos during the laboratory period
will be deferred immediately to continue the activity. At the same time, they will receive a grade of
ZERO for that particular activity.
 St. Scholastica’s College Tacloban
Maharlika Highway, Brgy. Campetic, Palo Leyte
DEPARTMENT OF MEDICAL LABORATORY SCIENCE
S.Y. 2021 – 2022

CLINICAL PARASITOLOGY COURSE REQUIREMENTS

Individual Requirements:
1. Laboratory gown with embossed/printed name on it
2. Prescribed laboratory manual
3. Goggles
4. Mask
5. Surgical Gloves
6. Pencil, Pen, and Art Materials
7. Calculator
8. Phlebotomy Kit
9. Alcohol/Hand sanitizer
10.Lecture Notebook

Group Requirements:
1. Rags
2. Tissue Paper
3. Masking/Labeling Tape
4. Black/Red Marker
5. Hand soap (liquid preferably)
6. Dish washing soap (liquid preferably)
7. Salad Cups (5 pcs)
8. Disinfectant spray or liquid (preferably Lysol)
9. Black garbage bag
10. Glass Bottles
11. Sugar (1/4 kilo)
 St. Scholastica’s College Tacloban
Maharlika Highway, Brgy. Campetic, Palo Leyte
DEPARTMENT OF MEDICAL LABORATORY SCIENCE
S.Y. 2021 – 2022

CLINICAL PARASITOLOGY

Laboratory Exercise 1

The Microscope
Name: __________________________________ Date: ___________________

Group Name: ____________________________ Score: __________________

Microscopic examination of feces and other specimen for parasitic analysis is best
performed when the laboratorian is knowledgeable about the types of microscopes available, their
primary characteristics, and the proper use and maintenance of these microscopes.

Bright-field microscopy is the most common type of microscopy performed in the

parasitology laboratory. Other types of microscopies that are useful for examining different minute
elements in the stool/urine/blood are phase contrast, polarizing, dark field, fluorescence, and
interference contrast. The type of microscopy used depends on the specimen type, the refractive
index of the object, and the ability to image unstained living cells. All microscopes are designed
to magnify small objects to such a degree that the details of their structure can be analyzed.

Essentially all types of microscopes contain a lens system, illumination system, and a body
consisting of a base, body tube, and nosepiece. Primary components of the lens system are the
oculars, the objectives, and the coarse- and fine-adjustment knobs. The illumination system
contains the light source, condenser, and field and iris diaphragms. Objects to be examined are
placed on a platform, referred to as the mechanical stage.

Materials:
1. Compound Light Microscope
2. Pen, pencil, and coloring materials

Study guide questions:

1. Draw and label the parts of a compound light microscope.
2. List down and explain the functions of at least 10 components/parts of a microscope:

3. Describe Köhler illumination and the microscope adjustment procedure used to ensure optimal
specimen imaging.

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4. Differentiate the first and secondary lens system of the microscope.

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5. Describe the daily care and preventive maintenance routines for microscopes. How should one
clean the microscope lenses from oil? How about from dust particles?

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6. Compare and contrast the principles and advantages of the following types of microscopies:

Brightfield: ____________________________________________________________________
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Phase-contrast: _________________________________________________________________
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Polarizing: ____________________________________________________________________
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Darkfield: _____________________________________________________________________
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Fluorescence: __________________________________________________________________
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Interference contrast: ____________________________________________________________
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a. Normansky: _____________________________________________________________
b. Hoffman: _______________________________________________________________

7. How do you convert a brightfield microscope to a darkfield microscope? To phase-contrast?

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8. Use operational terms in differentiating an aperture diaphragm from a condenser.

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9. What is refractive index? How does it affect the visibility of microscopic sediments?

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