WRITE ON APPLICATION OF AUTORADIOGRAPHY

Localization and detection of molecules with the use of

radioactive radiations and producing a photograph on the
photographic emulsion is known as autoradiography and the film
obtained is called autoradiograph.
Application of autoradiography
1. Auto-radiography is used to determine receptor distribution and localization while
studying neurodegenerative disorders. 2.
2. Application of auto-radiography in electrophoretic transfer of proteins from
polyacrylamide gels to nitro-cellulose sheets during blotting. 3. To study cytogenesis of
the fore-brain.
4. Applications in radio-pharmaceutical research.

5. Applications in radioimmunoelectroosmophoresis to study viruses.

6. In imaging and analysing rock porosity.
7. As a tool for genetic studies.
8. For comparison of complex mixtures of proteins.
9. Applications in microbial ecology. 10. Determining gross absorption and utilization of
foliar applied nutrients etc.
Today, auto-radiography is employed as an important detection tool for the
identification of different target receptors in various tissues to provide us with a better
understanding of molecular pharmacological pathways and also a great tool to help in the
study of the genetic.