Antibody panels for diagnosis, classification and

monitoring of hematological malignancies:

Composition and technical information on reagents

Version 1.10 (9 September 2022)

Published as Appendix in: Van Dongen JJM, Lhermitte L, Böttcher S, Almeida J, van der Velden VHJ,
Flores-Montero J, Rawstron A, Asnafi V, Lécrevisse Q, Lucio P, Mejstrikova E, Szczepański T, Kalina T, de
Tute R, Brüggemann M, Sedek L, Cullen M, Langerak AW, Mendonça A, Macintyre E, Martin-Ayuso M,
Hrusak O, Vidriales MB, Orfao A. EuroFlow antibody panels for standardized n-dimensional flow
cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 2012; 26:
1908-1975.

©EuroFlow
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Updated in Version 1.10 (Updates in blue boxes outlined in red)

• Corrections of small typing errors

• New clone for CD2 (panel 9 T-CLPD page 11)
• Titer change of CD138-BV421 (panel 11 MM-MRD panel page 13)

Disclaimer on EuroFlow reference reagents and equivalent reagents

EuroFlow reference reagents, obtained from multiple different companies, have been tested under specific
conditions for usage at specific concentrations in the EuroFlow antibody combinations as reported in the EuroFlow
documents. The recommended reagents aim to have comparable results in the (inter-laboratory) standardized
EuroFlow staining procedures. However, EuroFlow cannot be responsible for any change in the manufacturing of the
reagents, such as concentration, purity, dye/protein ratio, or any other factor that may affect the performance of
these products both as single reagents and in the combinations in which they are included.

The so-called “equivalent reagents” have been tested as single reagents in comparison with the EuroFlow reference
reagents. Performance of equivalent reagents in each of the individual EuroFlow 8-color combinations or
modifications in the performance characteristics of equivalent reagents (e.g. in different lots) is out of the EuroFlow
control.

©EuroFlow Page 2 of 14
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Panel 1. Composition of ALOT and technical information on reagents

PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

cyCD3 CD45 cyMPO cyCD79a CD34 CD19 CD7 smCD3

Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel µl/test
number
cyCD3 PacB UCHT1 BD Biosciences 558117 ALOT, T-ALL 7
smCD3 APCH7 SK7 BD Biosciences 641415 ALOT, T-ALL 3
CD7 APC 124-1D1 eBioscience 17-0079-42 ALOT, T-ALL, AML 2
ALOT, LST, SST, BCP-ALL, PCD, B-
CD19 PECy7 J3-119 Beckman Coulter IM3628 5
CLPD, MM-MRD, BCP-ALL
CD34 PerCPCy5.5 8G12 BD Biosciences 347222 ALOT, BCP-ALL, AML, BCP-ALL MRD 10
CD45 PacO HI30 Invitrogen MHCD4530 ALOT, LST, SST, BCP-ALL, T-ALL, 5
AML, B-CLPD, T-CLPD, NK-CLPD,
BCP-ALL MRD
cyCD79a PE HM57 Dako R7159 ALOT 5
cyMPO FITC MPO-7 Dako F0714 ALOT 3

©EuroFlow Page 3 of 14
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Panel 2. Composition of LST and technical information on reagents

PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

CD20 CD8 and CD56 and CD19 and
CD45 CD5 smCD3 CD38
and CD4 smIgλ smIgκ TCRγδ

Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel µl/test
number
smCD3 APC SK7 BD Biosciences 345767 LST, SST, B-CLPD 2.5
CD4 PacB RPA-T4 BioLegend 300521 LST, B-CLPD, T-CLPD 0.5
CD5 PerCPCy5.5 L17F12 BD Biosciences 341109 LST, T-ALL, B-CLPD 15
CD8 FITC UCH-T4 Cytognos CYT-SLPC-50 LST, B-CLPD 20 (Part of LST
mixture)
CD19 PECy7 J3-119 Beckman Coulter IM3628 LST, ALOT, SST, BCP-ALL, PCD, B- 5
CLPD, MM-MRD, BCP-ALL MRD
CD20 PacB 2H7 BioLegend 302320 LST, SST, BCP-ALL, B-CLPD, BCP- 1
ALL MRD
CD38 APCH7 HB7 BD Biosciences EU: 656646 LST, SST, BCP-ALL, T-ALL, AML, B- 3
US: 653314 CLPD
CD45 PacO HI30 Invitrogen MHCD4530 LST, ALOT, SST, BCP-ALL, T-ALL, 5
AML, B-CLPD, T-CLPD, NK-CLPD,
BCP-ALL MRD
CD56 PE C5.9 Cytognos CYT-SLPC-50 LST, B-CLPD 20 (Part of LST
mixture)
smIgκ PE polyclonal Cytognos CYT-SLPC-50 LST, B-CLPD 20 (Part of LST
mixture)
smIgλ FITC polyclonal Cytognos CYT-SLPC-50 LST, B-CLPD 20 (Part of LST
mixture)
EU: 655410
TCRγδ PECy7 11F2 BD Biosciences LST, B-CLPD 3
US: 655434

©EuroFlow Page 4 of 14
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Panel 3. Composition of SST and technical information on reagents

PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

CD8 and CD56 and smCD3 and
CD20 CD45 CD4 CD19 CD38
smIgλ smIgκ CD14

Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel µl/test
number
smCD3 APC SK7 BD Biosciences 345767 SST, LST, B-CLPD 2.5
CD4 PerCPCy5.5 SK3 Cytognos CYT-SLPC4-50 SST 20 (Part of SST
mixture)
CD8 FITC UCH-T4 Cytognos CYT-SLPC4-50 SST 20 (Part of SST
mixture)
CD14 APC MφP9 BD Biosciences 345787 SST 5
CD19 PECy7 J3-119 Beckman Coulter IM3628 SST, ALOT, LST, BCP-ALL, PCD, B- 5
CLPD, MM-MRD, BCP-ALL MRD
CD20 PacB 2H7 BioLegend 302320 SST, LST, BCP-ALL, B-CLPD, BCP- 1
ALL MRD
CD38 APCH7 HB7 BD Biosciences EU: 656646 3
SST, LST, BCP-ALL, AML, B-CLPD
US: 653314
CD45 PacO HI30 Invitrogen MHCD4530 SST, ALOT, LST, BCP-ALL, T-ALL, 5
AML, B-CLPD, T-CLPD, NK-CLPD,
BCP-ALL MRD
CD56 PE C5.9 Cytognos CYT-SLPC4-50 SST 20 (Part of SST
mixture)
smIgκ PE polyclonal Cytognos CYT-SLPC4-50 SST 20 (Part of SST
mixture)
smIgλ FITC polyclonal Cytognos CYT-SLPC4-50 SST 20 (Part of SST
mixture)

©EuroFlow Page 5 of 14
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Panel 4. Composition of PCD and technical information on reagents

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCC750

1 CD45 CD138 CD38 CD56 β2micro CD19 cyIgκ cyIgλ

APCH7
2 CD45 CD138 CD38 CD28 CD27 CD19 CD117 CD81

Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel µl/test
number
PCD, ALOT, LST, SST, BCP-ALL, B-
CD19 PECy7 J3-119 Beckman Coulter IM3628 5
CLPD, MM-MRD, BCP-ALL MRD
CD27 PerCPCy5.5 L128 BD Biosciences EU: 656643 PCD
20
US: 655429
CD28 PE L293 BD Biosciences 348047 PCD 20

}
CD38 FITC LD38 Cytognos CYT-38F PCD 3*
CD38 pure LD38 Cytognos CYT-38P1 PCD 2* 5*

CD45 PacB T29/33 Dako PB986 PCD 5

CD56 PE C5.9 Cytognos CYT-56PE PCD, AML, MM-MRD 5
CD81 APCH7 JS-81 BD Biosciences EU: 656647 PCD, BCP-ALL, B-CLPD 5
US: 656154
CD117 APC 104D2 BD Biosciences 333233 PCD, BCP-ALL, MM-MRD 5
CD138 PacO B-A38 Exbio PO-520 PCD 4
β2micro PerCPCy5.5 Tü99 BD Biosciences EU: 656645 PCD
15
US: 655435
cyIgκ APC polyclonal Dako C0222 PCD, MM-MRD 2.5
rabbit serum
cyIgλ APCC750 polyclonal Cytognos CYT-LAC750 PCD, BCP-ALL, MM-MRD 3
* Mixture of fluorochrome-conjugated and –unconjugated antibodies is used to reduce the signal intensity, while retaining saturation
conditions to avoid unpredictable variation in staining patterns.

©EuroFlow Page 6 of 14
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Panel 5. Composition of BCP-ALL and technical information on reagents

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

1 CD20 CD45 CD58 CD66c CD34 CD19 CD10 CD38
APCC750
smIgM and
2 smIgκ CD45 cyIgµ CD33 CD34 CD19 smIgλ
CD117
APCH7
3 CD9 CD45 nuTdT CD13 CD34 CD19 CD22 CD24
CD15 and
4 CD21 CD45 NG2 CD34 CD19 CD123 CD81
CD65

Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel µl/test
number
CD9 PacB MEM-61 Exbio PB-208-T100 BCP-ALL 4
CD10 APC HI10A BD Biosciences 332777 BCP-ALL, BCP-ALL MRD 5
CD13 PE L138 BD Biosciences 347406 BCP-ALL, T-ALL, AML 7
CD15 FITC MMA BD Biosciences 332778 BCP-ALL, AML 10
CD19 PECy7 J3-119 Beckman Coulter IM3628 BCP-ALL, ALOT, LST, SST, PCD, B-CLPD, 5
MM-MRD, BCP-ALL MRD
CD20 PacB 2H7 BioLegend 302320 BCP-ALL, LST, SST, B-CLPD, BCP-ALL MRD 1
CD21 PacB LT21 Exbio PB-306-T100 BCP-ALL 4
CD22 APC S-HCL-1 BD Biosciences 333145 BCP-ALL 5
CD24 APCH7 ML5 BD Biosciences EU: 658331 BCP-ALL 5
CD33 PE P67.6 BD Biosciences 345799 BCP-ALL 5
CD34 PerCPCy5.5 8G12 BD Biosciences 347222 BCP-ALL, ALOT, AML, BCP-ALL MRD 10
CD38 APCH7 HB7 BD Biosciences EU: 656646 BCP-ALL, LST, SST, AML, B-CLPD 3
US: 653314
CD45 PacO HI30 Invitrogen MHCD4530 BCP-ALL, ALOT, LST, SST, T-ALL, AML, T- 5
CLPD, NK-CLPD, BCP-ALL MRD
CD58 FITC 1C3 BD Biosciences 555920 BCP-ALL 7
CD65 FITC 88H7 Beckman Coulter B36299 BCP-ALL 7
CD66c PE KOR-SA3544 Beckman Coulter IM2357U BCP-ALL, BCP-ALL MRD 10
CD81 APCH7 JS-81 BD Biosciences EU: 656647 BCP-ALL, PCD, B-CLPD 5
US: 656154
CD117 APC 104D2 BD Biosciences 333233 BCP-ALL, PCD, MM-MRD 5
CD123 APC AC145 Miltenyi Biotec 130-113-322 BCP-ALL, T-ALL, AML 1
smIgκ PacB A8B5 Exbio PB-504-T100 BCP-ALL 4
smIgλ APCC750 polyclonal Cytognos CYT-LAC750 BCP-ALL, PCD, MM-MRD 3
cyIgµ FITC polyclonal Dako F0058 BCP-ALL 10
rabbit serum
smIgM APC G20-127 BD Biosciences 551062 BCP-ALL, B-CLPD 10
NG2 PE 7.1 Beckman Coulter B92429 BCP-ALL, AML 10
nuTdT FITC HT-6 Dako F7139 BCP-ALL, T-ALL, AML 10

©EuroFlow Page 7 of 14
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Panel 6. Composition of T-ALL and technical information on reagents

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

1 cyCD3 CD45 nuTdT CD99 CD5 CD10 CD1a smCD3

2 cyCD3 CD45 CD2 CD117 CD4 CD8 CD7 smCD3
3 cyCD3 CD45 TCRγδ TCRαβ CD33 CD56 cyTCRβ smCD3
4 cyCD3 CD45 CD44 CD13 HLADR CD45RA CD123 smCD3

Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel µl/test
number
CD1a APC HI149 BD Biosciences 559775 T-ALL 5
CD2 FITC RPA-2.10 BD Biosciences 555326 T-ALL 5
cyCD3 PacB UCHT1 BD Biosciences 558117 T-ALL, ALOT 7
smCD3 APCH7 SK7 BD Biosciences 641415 T-ALL, ALOT 3
CD4 PerCPCy5.5 SK3 BD Biosciences 332772 T-ALL 7
CD5 PerCPCy5.5 L17F12 BD Biosciences 341109 T-ALL, LST, B-CLPD 15
CD7 APC 124-1D1 eBioscience 17-0079-42 T-ALL, ALOT, AML 2
CD8 PECy7 SFCI21Thy2D3 Beckman Coulter 737661 T-ALL 5
CD10 PECy7 HI10A BD Biosciences 341112 T-ALL 5
CD13 PE L138 BD Biosciences 347406 T-ALL, BCP-ALL, AML 7
CD33 PerCPCy5.5 P67.6 BD Biosciences 333146 T-ALL 10
CD44 FITC L178 BD Biosciences 347943 T-ALL 7
CD45 PacO HI30 Invitrogen MHCD4530 T-ALL, ALOT, LST, SST, BCP-ALL, AML, T- 5
CLPD, NK-CLPD, BCP-ALL MRD
CD45RA PECy7 L48 BD Biosciences 337186 T-ALL 5
CD56 PECy7 N901 Beckman Coulter A21692 T-ALL, NK-CLPD 5
CD99 PE Tü12 BD Biosciences 555689 T-ALL 5
CD117 PE 104D2 BD Biosciences 332785 T-ALL 5
CD123 APC AC145 Miltenyi Biotec 130-113-322 T-ALL, BCP-ALL, AML 1
HLA-DR PerCPCy5.5 L243 BD Biosciences 552764 T-ALL, B-CLPD 10
TCRαβ PE IP26A Beckman Coulter A39499 T-ALL 7
cyTCRβ APC 8A3 (βF1) Cytognos CYT-BF1AP T-ALL 3
TCRγδ FITC IMMU510 Beckman Coulter IM1571U T-ALL 10
nuTdT FITC HT-6 Dako F7139 T-ALL, BCP-ALL, AML 10

©EuroFlow Page 8 of 14
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Panel 7. Composition of AML/MDS and technical information on reagents

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7
1 HLADR CD45 CD16 CD13 CD34 CD117 CD11b CD10
2 HLADR CD45 CD35 CD64 CD34 CD117 CD300e CD14
3 HLADR CD45 CD36 CD105 CD34 CD117 CD33 CD71
4 HLADR CD45 nuTdT CD56 CD34 CD117 CD7 CD19
5 HLADR CD45 CD15 NG2 CD34 CD117 CD22 CD38
CD42a and
6 HLADR CD45 CD203c CD34 CD117 CD123 CD4
CD61
7 HLADR CD45 CD41a CD25 CD34 CD117 CD42b CD9
Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel µl/test
number
CD4 APCH7 SK3 BD Biosciences 641398 AML 5
CD7 APC 124-1D1 eBioscience 17-0079-42 AML, ALOT, T-ALL 2
CD9 APCH7 M-L13 BD Biosciences EU: 655409 AML 10
CD10 APCH7 HI10A BD Biosciences EU: 655404 AML
5
US: 655426
CD11b APC D12 BD Biosciences 333143 AML 5
CD13 PE L138 BD Biosciences 347406 AML, BCP-ALL, T-ALL 7
CD14 APCH7 MφP9 BD Biosciences 641394 AML 5
CD15 FITC MMA BD Biosciences 332778 AML, BCP-ALL 10
CD16 FITC CLB Fc gran/1, 5D2 Sanquin M1604 AML 20
CD19 APCH7 SJ25C1 BD Biosciences 641395 AML, NK-CLPD 5
CD22 APC S-HCL-1 BD Biosciences 333145 AML, BCP-ALL 5
CD25 PE 2A3 BD Biosciences 341011 AML, T-CLPD, NK-CLPD 10
CD33 APC P67.6 BD Biosciences 345800 AML 10
CD34 PerCPCy5.5 8G12 BD Biosciences 347222 AML, ALOT, BCP-ALL, BCP-ALL MRD 10
CD35 FITC E11 BD Biosciences 555452 AML 5
CD36 FITC CLB-IVC7 Sanquin M1613 AML 5
CD38 APCH7 HB7 BD Biosciences EU: 656646 AML, LST, SST, BCP-ALL, B-CLPD 3
US: 653314
CD41a FITC HIP8 BD Biosciences EU: 333147 AML 20
US: 340929
CD42a FITC GRP-P Serotec MCA1227F AML 1
CD42b APC HIP1 BD Biosciences 551061 AML 1
CD45 PacO HI30 Invitrogen MHCD4530 AML, ALOT, LST, SST, BCP-ALL, T- 5
ALL, B-CLPD, T-CLPD,NK-CLPD, BCP-
ALL MRD
CD56 PE C5.9 Cytognos CYT-56PE AML, PCD, MM-MRD 5
CD61 FITC RUU-PL7F12 BD Biosciences 347407 AML 4
CD64 PE 10.1 BD Biosciences 644385 AML 20
CD71 APCH7 M-A712 BD Biosciences EU: 655408 AML
2
US: 655431
CD105 PE 266 BD Biosciences 560839 AML 5
CD117 PECy7 104D2D1 Beckman Coulter EU: B49221 AML 5
IM3698
CD123 APC AC145 Miltenyi Biotec 130-113-322 AML, BCP-ALL, T-ALL 1
CD203c PE 97A6 Beckman Coulter B92404 AML 10
CD300e APC UP-H2 Immunostep IREM2A-T100 AML 5
HLADR PacB L243 BioLegend 307624 AML, NK-CLPD 1 (1:5
dilution)
NG2 PE 7.1 Beckman Coulter B92429 AML, BCP-ALL 10
nuTdT FITC HT-6 Dako F7139 AML, BCP-ALL, T-ALL 10

©EuroFlow Page 9 of 14
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Panel 8. Composition of B-CLPD and technical information on reagents

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7
CD20 CD8 and CD56 and CD19 and
1 CD45 CD5 smCD3 CD38
and CD4 smIgλ smIgκ TCRγδ
2 CD20 CD45 CD23 CD10 CD79b CD19 CD200 CD43
3 CD20 CD45 CD31 CD305 CD11c CD19 smIgM CD81
4 CD20 CD45 CD103 CD95 CD22 CD19 CD185 CD49d
5 CD20 CD45 CD62L CD39 HLADR CD19 CD27
Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel µl/test
number
smCD3 APC SK7 BD Biosciences 345767 B-CLPD, SST, LST 2.5
CD4 PacB RPA-T4 BioLegend 300521 B-CLPD, LST, T-CLPD 0.5
CD5 PerCPCy5.5 L17F12 BD Biosciences 341109 B-CLPD, LST, T-ALL 15
CD8 FITC UCH-T4 Cytognos CYT-SLPC-50 B-CLPD, LST 20 (part of LST
mixture)
CD10 PE ALB1 Beckman Coulter A07760 B-CLPD 20
CD11c PerCPCy5.5 B-Ly6 BD Biosciences 658330 B-CLPD 10
CD19 PECy7 J3-119 Beckman Coulter IM3628 B-CLPD, ALOT, LST, SST, BCP-ALL, PCD, 5
MM-MRD, BCP-ALL MRD
CD20 PacB 2H7 BioLegend 302320 B-CLPD, LST, SST, BCP-ALL, BCP-ALL 1
MRD
CD22 PerCPCy5.5 S-HCL-1 BD Biosciences 658329 B-CLPD 25
CD23 FITC MHM6 Dako F7062 B-CLPD 2.5
CD27 APC L128 BD Biosciences 337169 B-CLPD 2.5
CD31 FITC WM59 BD Biosciences 555445 B-CLPD 10
CD38 APCH7 HB7 BD Biosciences EU: 656646 B-CLPD, LST, SST, BCP-ALL, AML 3
US: 653314
CD39 PE TÜ66 BD Biosciences 555464 B-CLPD 10
CD43 APCH7 IG10 BD Biosciences EU: 655407 B-CLPD
2.5
US: 655430
CD45 PacO HI30 Invitrogen MHCD4530 B-CLPD, ALOT, LST, SST, BCP-ALL, T- 5
ALL, AML, T-CLPD, NK-CLPD, BCP-ALL
MRD
CD49d APCH7 9F10 BD Biosciences EU: 658332 B-CLPD 1
CD56 PE C5.9 Cytognos CYT-SLPC-50 B-CLPD, LST 20 (part of LST
mixture)
CD62L FITC SK11 BD Biosciences 347443 B-CLPD 2.5
CD79b PerCPCy5.5 SN8 BD Biosciences 656644 B-CLPD 10
CD81 APCH7 JS-81 BD Biosciences EU: 656647 B-CLPD, PCD, BCP-ALL 5
US: 656154
CD95 PE DX2 BD Biosciences 555674 B-CLPD 20
CD103 FITC Ber-ACT8 BD Biosciences 333155 B-CLPD 2
CD185 APC 51505 R&D Systems FAB190A B-CLPD 10
CD200 APC OX104 eBioscience 17-9200 B-CLPD 1.25
CD305 PE DX26 BD Biosciences 550811 B-CLPD 10
HLA-DR PerCPCy5.5 L243 BD Biosciences 552764 B-CLPD, T-ALL 10
smIgk PE polyclonal Cytognos CYT-SLPC-50 B-CLPD, LST 20 (part of LST
mixture)
smIgλ FITC polyclonal Cytognos CYT-SLPC-50 B-CLPD, LST 20 (part of LST
mixture)
smIgM APC G20-127 BD Biosciences 551062 B-CLPD, BCP-ALL 10
TCRγδ PECy7 11F2 BD Biosciences EU: 655410 B-CLPD, LST
3
US: 655434

©EuroFlow Page 10 of 14
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Panel 9. Composition of T-CLPD and technical information on reagents

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

1 CD4 CD45 CD7 CD26 smCD3 CD2 CD28 CD8
2 CD4 CD45 CD27 CD197 smCD3 CD45RO CD45RA CD8
3 CD4 CD45 CD5 CD25 smCD3 HLADR cyTcL1 CD8
4 CD4 CD45 CD57 CD30 smCD3 CD11c CD8
5 CD4 CD45 cyPerforin cyGranzyme B smCD3 CD16 CD94 CD8
6 CD4 CD45 CD279 smCD3 CD8

Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel µl/test
number
CD2 PECy7 L303.1 BD Biosciences 335821 T-CLPD 2
smCD3 PerCPCy5.5 SK7 BD Biosciences 332771 T-CLPD, NK-CLPD 10
CD4 PacB RPA-T4 BioLegend 300521 T-CLPD, LST, B-CLPD 0.5
CD5 FITC L17F12 BD Biosciences 345781 T-CLPD 10
CD7 FITC 4H9 BD Biosciences 347483 T-CLPD, NK-CLPD 10
CD8 APCH7 SK1 BD Biosciences 641400 T-CLPD 5
CD11c APC S-HCL-3 BD Biosciences 333144 T-CLPD, NK-CLPD 2
CD16 PECy7 3G8 BD Biosciences 557744 T-CLPD 2
CD25 PE 2A3 BD Biosciences 341011 T-CLPD, AML, NK-CLPD 10
CD26 PE L272 BD Biosciences 340423 T-CLPD, NK-CLPD 10
CD27 FITC L128 BD Biosciences 340424 T-CLPD 10
CD28 APC CD28.2 BD Biosciences 559770 T-CLPD 10
CD30 PE BerH8 BD Biosciences 550041 T-CLPD 10
CD45 PacO HI30 Invitrogen MHCD4530 T-CLPD, ALOT, LST, SST, BCP-ALL, T- 5
ALL, AML, NK-CLPD, BCP-ALL MRD
CD45RA APC HI100 BD Biosciences 550855 T-CLPD 10
CD45RO PECy7 UCHL1 BD Biosciences 337168 T-CLPD 2
CD57 FITC HNK-1 BD Biosciences 333169 T-CLPD, NK-CLPD 10
CD94 APC HP-3D9 BD Biosciences 559876 T-CLPD, NK-CLPD 5
CD197 PE 3D12 eBioscience 12-1979 T-CLPD 10
CD279 PE MIH4 BD Biosciences 557946 T-CLPD 20
cyGranzyme B PE GB11 BD Biosciences 561142 T-CLPD, NK-CLPD 5
HLADR PECy7 L243 BD Biosciences 335830 T-CLPD 2.5
cyPerforin FITC δG6 BD Biosciences 556577 T-CLPD, NK-CLPD 10
cyTCL1 APC eBio1-21 eBioscience 17-6699 T-CLPD 2

©EuroFlow Page 11 of 14
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Panel 10. Composition of NK-CLPD and technical information on reagents

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCH7

1 CD2 CD45 CD7 CD26 smCD3 CD56 CD5 CD19
2 CD16 CD45 CD57 CD25 smCD3 CD56 CD11c CD19
3 HLADR CD45 cyPerforin cyGranzyme B smCD3 CD56 CD94 CD19

Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel µl/test
number
CD2 PacB TS1/8 BioLegend 309216 NK-CLPD 1
smCD3 PerCPCy5.5 SK7 BD Biosciences 332771 NK-CLPD, T-CLPD 10
CD5 APC L17F12 BD Biosciences 345783 NK-CLPD 2.5
CD7 FITC 4H9 BD Biosciences 347483 NK-CLPD, T-CLPD 10
CD11c APC S-HCL-3 BD Biosciences 333144 NK-CLPD, T-CLPD 2
CD16 PacB 3G8 BioLegend 302032 NK-CLPD 5
CD19 APCH7 SJ25C1 BD Biosciences 641395 NK-CLPD, AML 5
CD25 PE 2A3 BD Biosciences 341011 NK-CLPD, AML, T-CLPD 10
CD26 PE L272 BD Biosciences 340423 NK-CLPD, T-CLPD 10
CD45 PacO HI30 Invitrogen MHCD4530 NK-CLPD, ALOT, LST, SST, BCP- 5
ALL, T-ALL, AML, T-CLPD, BCP-
ALL MRD
CD56 PECy7 N901 Beckman Coulter A21692 NK-CLPD, T-ALL 5
CD57 FITC HNK-1 BD Biosciences 333169 NK-CLPD, T-CLPD 10
CD94 APC HP-3D9 BD Biosciences 559876 NK-CLPD, T-CLPD 5
cyGranzyme B PE GB11 BD Biosciences 561142 NK-CLPD, T-CLPD 5
HLADR PacB L243 BioLegend 307624 NK-CLPD, AML 1 (1:5 dilution)
cyPerforin FITC δG6 BD Biosciences 556577 NK-CLPD, T-CLPD 10

©EuroFlow Page 12 of 14
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Panel 11. Composition of MM-MRD panel and technical information on reagents*

Tube BV421 BV510 FITC PE PerCPCy5.5 PECy7 APC APCC750

1 CD138 CD27 CD38 CD56 CD45 CD19 CD117 CD81
2 CD138 CD27 CD38 CD56 CD45 CD19 cyIgκ cyIgλ

Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel µl/test
number
Beckmann MM-MRD, ALOT, LST, SST, BCP-
CD19 PECy7 J3-119 IM3628 5
Coulter ALL, PCD, B-CLPD, BCP-ALL MRD
CD27 BV510 O323 BioLegend 302835 MM-MRD 10
CD38 FITC Multi-epitope Cytognos CYT-38F2 MM-MRD 6
CD45 PerCPCy5.5 HI30 BioLegend 304028 MM-MRD 10
CD56 PE C5.9 Cytognos CYT-56PE MM-MRD, PCD, AML 2
CD81 APCC750 M38 Cytognos CYT-81AC750 MM-MRD 6
CD117 APC 104D2 BD Biosciences 333233 MM-MRD, PCD, BCP-ALL 5
CD138 BV421 MI15 BD Biosciences 562935 MM-MRD 3a
cyIgκ APC Polyclonal Dako C0222 MM-MRD, PCD 5
cyIgλ APCC750 Polyclonal Cytognos CYT-LAC750 MM-MRD, PCD, BCP-ALL 3
a
, reassessed after modification on the format of the reagent. Current lot concentration (as per this document effective date) is
0.1µg/µl according to manufacturer’s documentation

* Flores-Montero J, Sanoja L, Paiva B, Puig N, García-Sánchez O, Böttcher S, van der Velden VHJ, Pérez-Morán JJ, Vidriales MB, García-
Sanz R, Jimenez C, González M, Matínez-López J, Corral A, Grigore GE, Fluxá R, Pontes R, Caetano J, Sedek L, del Cañizo MC, Bladé J,
Lahuerta JJ, Aguilar C, Bárez A, García-Mateo A, Labrador J, Leoz P, Aguilera-Sanz C, San Miguel J, Mateos MV, Durie B, van Dongen JJM,
Orfao A, on behalf of the EuroFlow Consortium and the International Myeloma Foundation. Next Generation Flow (NGF) for Highly
Sensitive and Standardized Detection of Minimal Residual Disease in Multiple Myeloma, Leukemia in press.

©EuroFlow Page 13 of 14
Updated: 9 September 2022 EuroFlow antibody panels version 1.10

Panel 12. Composition of BCP-ALL MRD panel and technical information on

reagents*

Tube PacB PacO FITC PE PerCPCy5.5 PECy7 APC APCA750

CD66c +
1 CD20 CD45 CD81 CD34 CD19 CD10 CD38
CD123
CD304 +
2 CD20 CD45 CD81 CD34 CD19 CD10 CD38
CD73

Catalogue
Marker Fluorochrome Clone Source Application in EuroFlow panel µl/test
number
CD10 APC HI10a BD Biosciences 332777 BCP-ALL MRD, BCP-ALL 5
Beckmann BCP-ALL MRD, MM-MRD, ALOT,
CD19 PECy7 J3-119 IM3628 5
Coulter LST, SST, BCP-ALL, PCD, B-CLPD
CD20 PacB 2H7 BioLegend 302320 BCP-ALL MRD, LST, SST, BCP-ALL, 1
B-CLPD
CD34 PerCPCy5.5 8G12 BD Biosciences 347222 BCP-ALL MRD, ALOT, BCP-ALL,
7
AML
CD38 APCA750 LS198-4-3 Coulter A86049 BCP-ALL MRD 1
CD45 PacO HI30 Invitrogen MHCD4530 BCP-ALL MRD, ALOT, LST, SST, 5
BCP-ALL, T-ALL, AML, B-CLPD,
T-CLPD, NK-CLPD
CD66c PE KOR-SA3544 Coulter IM2357U BCP-ALL MRD, BCP-ALL 10
CD73 PE AD-2 BD Pharmingen 550257 BCP-ALL MRD 20
CD81 FITC JS-81 BD Biosciences 551108 BCP-ALL MRD 5
CD123 PE AC145 Miltenyi 130-113-326 BCP-ALL MRD 2
CD304 PE 12C2 BioLegend 354504 BCP-ALL MRD 5

* Theunissen P, Mejstrikova E, Sedek L, van der Sluijs A, Gaipa G, Bartels M, Sobral da Costa E, Kotrová M, Novakova M,
Sonneveld E, Buracchi C, Bonaccorso P, Oliveira E, te Marvelde J, Szczepanski T, Lhermitte L, Hrusak O, Lecrevisse Q,
Grigore GE, Froňkova E, Trka J, Brüggemann M, Orfao A, van Dongen JJM, van der Velden VHJ, on behalf of the EuroFlow
Consortium. Standardized next-generation flow cytometry for highly sensitive MRD measurements in B-cell acute lymphoblastic
leukemia. Blood 2017; 129: 347-357.

©EuroFlow Page 14 of 14