Gene of interest 1

Mice Groups
Animal 1 Control 1
Animal 2 Control 2
Animal 3 Control 3
Animal 4 Disease 1
Animal 5 Disease 2
Animal 6 Disease 3
Animal 7 Clinda 1
Animal 8 Clinda2
Animal 9 Clinda3

Gene of interest 2

Mice Groups
Animal 1 Control 1
Animal 2 Control 2
Animal 3 Control 3
Animal 4 Disease 1
Animal 5 Disease 2
Animal 6 Disease 3
Animal 7 Clinda 1
Animal 8 Clinda2
Animal 9 Clinda3
Mean value of triplicates dct ddct = (Expdct - Con_dct) Average of Control dct
Target 1 (ct) Ref (ct) Target-ref. Exp-Control (avg.)
32.7176593927777 20.326650105 12.391009288
32.7713462123514 19.741754566 13.029591647
32.8137290648425 20.057162866 12.756566199
32.9836467461036 20.069331921 12.914314825 0.188592447073173 12.7257223776569
32.506273977599 20.577177224 11.929096754 -0.79662562383113 12.7257223776569
33.4639672639637 20.03669040377 13.42727686 0.701554482541422 12.7257223776569
30.2635448586637 20.23286772 10.030677138 -2.69504523948028 12.7257223776569
30.2635448586637 20.378297202 9.8852476569 -2.84047472073338 12.7257223776569
32.0571628662043 20.07537451158 11.981788355 -0.743934023029279 12.7257223776569

Mean value of triplicates dct ddct = (Expdct - Con_dct) Average of Control dct
Target 2 Ref Target-ref. Exp-Control (avg.)
31.1837736661416 20.326650105 10.85712356088
30.1901556151367 19.741754566 10.44840104961
30.2159302320111 20.057162866 10.15876736581
33.9041393272051 20.069331921 13.83480740583 3.34671008040213 10.4880973254295
33.3079664276439 20.577177224 12.73078920387 2.24269187844123 10.4880973254295
33.129436137494 20.03669040377 13.09274573373 2.60464840829913 10.4880973254295
31.0283433107752 20.23286772 10.79547559029 0.307378264858682 10.4880973254295
31.7022808724813 20.378297202 11.32398367074 0.835886345311684 10.4880973254295
32.3325815826107 20.07537451158 12.25720707103 1.76910974560453 10.4880973254295
 12.39101
13.02959
2(ddct) 12.75657
Fold change 12.72572

Log2 fold Change

0.8774613914 -0.188592 Disease 1 Disease 2 Disease 3 Clinda 1 Clinda2 Clinda3
1.7370335554 0.796626 -0.1885924 0.796626 -0.701554 2.695045 2.840475 0.743934
0.6149092939 -0.701554
6.475740793 2.695045 Disease Clinda 2.5

Log 2 Fold change (Gene of interest

7.1625570287 2.840475 Avg. -0.0311738 2.093151
2
1.6747363846 0.743934 SD 0.7613944 1.170717
SEM 0.4395912 0.675914 1.5
* I used SEM for Error bars
1
1)

0.5
10.85712 0
10.4484 Disease Clinda
2(ddct) 10.15877 -0.5
Fold change 10.4881 Axis Title

Log2 fold Change

0.098296912981 -3.3467101
0.211291717615 -2.2426919
0.164407907139 -2.6046484
0.808108960336 -0.3073783
0.560238740645 -0.8358863
0.293389725607 -1.7691097
 Clinda

Axis Title