1. Check the pressure:
Check A, B, C & L eluent, if they are enough;
H8 Operation SOP Flow Chart
3-12Mpa.
2. Check the ADC value:
415nm:16,000~18,000;
500nm:8,000~10,000.
Prepare QC sample:
1. Take out QC from refrigerator, wait it
until room temperature.
2. Use pipette inject 100ul distilled
water or de-ionized water into QC
bottle.
3. Wait 30 minutes until it fully re-
dissolve.
4. Aliquot the re-dissolve sample, 10ul in
each EP tube, 1 bottle can aliquot in 10
EP tube.
Doing QC:
1. Use pipette inject 1,500ul L eluent
into EP tube, mixed it well;
2. Input QC information in QC interface;
3. Place the samples inside sample rack,
the sequence is: WB, QC1, WB and QC2.
4. Doing the Auto QC in QC interface.
TIPS:
1. The aliquot sample needs to storage in
-20℃, it can storage for 14 days;
2. QC bottle need to storage at 2~8℃.
Daily Maintenance
1. Check the liquid level of each bottle of
reagent and waste bottle, replace and
empty the waste liquid if necessary.
2. Check remaining number of columns,
filters and replace them if necessary.
Weekly Maintenance
1. Cleaning sample rack and instrument
surface.
2. Maintain the cleanliness of the
warehouse.
3. Cleaning screen.
All of maintenance suggest to wipe with a
soft, disposable towel or fabric.
Monthly Maintenance
1. Clean the Diluting Pool once
Use a cotton swab with 0.5% sodium
hypochlorite to manually clean the
diluting pool
2. Sampling probe cleaning
Clean and maintain the sampling probe
once a month. Open the right board and
wipe the surface with a soft cloth or
sponge dipped with 5‰ sodium
hypochlorite solution.
Note: Check the status of the sampling
probe, and replace it if the sampling is
inaccurate or obviously bent.
: Necessary steps
: Optional steps
1.
2. Check the Waste liquid bucket, if it was
empty;
3. Check the reagent bottle, if any condensate
Check before start up the machine water inside, it need shaking well.
Prepare Calibrator:
1. Take out calibrator from refrigerator, wait
Start manually Start automatically it until room temperature.
2. Use pipette inject 100ul distilled water
or de-ionized water into calibrator bottle.
3. Wait 30 minutes until it fully re-dissolve.
4. Aliquot the re-dissolve sample, 10ul in
each EP tube, 1 bottle can aliquot in 10 EP
tubes.
Confirm machine statement
Doing Calibration:
1. Use pipette inject 1,500ul L eluent into
EP tube, mixed it well;
2. Input calibration information in
calibration interface;
3. Place the samples inside sample rack, the
Doing calibration
sequence is: WB, C1, WB and C2.
4. Doing the calibration in Cal. interface.
TIPS:
1. The aliquot sample needs to storage in -
20℃, it can storage for 14 days;
2. Calibrator bottle need to storage at
Doing daily QC 2~8℃.
Mixed the whole blood sample, place the
blood sample in the sample rack, and click the
analysis interface "Start "to start the test;
Doing sample test
TIPS:
1. Samples taken from the refrigerator need to
stand at room temperature for 30 minutes.
Test after mixing
2. Select the anticoagulated fresh blood
sample of the day, in normal temperature, it
should not exceed 48 hours;
Add more sample inside
3. If testing diluted blood, in first position it
must put a whole blood sample, EP tube hat
needs to be cut off.
Wait until the test is finished, replace the
Result checking
sample rack
Check if the graph is good and no baseline
drift (This is a standard graph):
Maintenance operation
Shutdown the machine