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Urt o 2

C) SAd (onning VecoA Tetii u

endonudeus DNA

a
Cloning
tchrA
Small SmallPeu 06- DNA

rm
H
Co bs qtabk maintoiao gahism_rd
hich a higa DNA fraqms
b f Uoning PunPoed S
na b DNA takan aUruh
a
ha
of DJanlsm 01pln
of badeniurn
lP
D =By Yating A Ve tor Small foYes
DNA l ŠCstYiiki ym
t
DN A
el

Plasmid A plasmid i a Small_tttacki-

-mosoma DNA mokitul!es_
Haat Phsitaly epanate
ew

Yam Uromostb
DNA ond Can YPutake indPedsd
Thy Smal len doub -Stded Dyt
malutulh in hackeia,-
ar

OCahqugahua pladnils-
C

Contoin Sck ot trantta

Coniugad e
c Prmak S
blu_itfk Durina Lonung
Plasn d
to ahathen Via Scxp

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NonSanggatius Plasmids- n incapablei s

ANIaking Congeon ku
Can ba tYanfeeme

a
Linqqadius Plmc

rm
Fenh B KE Fladics apoblof langugoionian
ult teexpYedionaf Scx p'R

Risae(0 Plasmidks=Cordoin Jes Aat Pui

ha
YaisaU agsidtanbiohu oY pos

Cok-Plasmi orteagu he kaun Prsks

lP
Can

Ddahu Pla ds Eahu dy eahs t

Substcnte
el

Valun flad muos1 mh balterim

Pathoga-
ew

Kestricdon ndapusdeassh
ar

A_Resti ution 3Jm Ytstriction donudeus, oY

n nzm aat ugthaves DNA
C

p n mo Cules Knooh o Ycstrdion

On lass ot
ORotthon
bTo adun do nu leat

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AYi hon enzqm an Comn classi has

Ke
Ido
CAn
diffoust ur S+Yuctun

a
DNA SubtYak
thun h t khr

rm
cut Tccognki

To DNA a YebtYickon enymdnak

nusion
Phos Pho oatKbon C ea trand)
Sus
hux.

ha
0+ DN A doublu

e YetYihon edanuleases
lP
Nahoal OCLLninF Yedtricton do nuclabcA GA

Cadegriud
bad on thuriComposlion andilen2imOtuth
el

XequYrmints natunuof uYtngit Scqulh

Ae Positton ot ub DNA lkavaa sle

and
ew

cav a sikax Yemok fYom

YLLoyntion s quirA botaiiAP Gnd

Sadenol-L mtwonin un ior
ar

Tbr-T Enmd kaus wAin oattt shert cheuh

de stanc fH a YLasihe Sik
C

1brl- Cnm ckavz at sks_a Shot distuu

rom
Comp ux
of &
aid a mo.duficoch on ylk

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Poge

TA V Enma kmodi tidONA g- mlol

AX -únd
DNA

a
DNA InASE

rm
DNA s speuku-
e of Cn2m ga wc fauliatis
Catning
DNA
formaion
SHManda
of
Pos
jcHhn
Phodieton
-
ha
bond Pla Yol in Ypainingsin
SAYad bYcakaincduplaxu NAj
O7ansm
T fiYk DNA Las
lP
thona taiud in1A62 inlenkLkma
achands0n an Hu t2 ab oYtorleš
el

1 Th E-Coli DA o ecodad
ew

2 T T DNA a f¥omhactonioPh Ta
ar

La batrioPhaga thatnfect Escheichia ol

ba va
C

3 Ih nammals Spcuhe tyPe

mammalun l
of

DNA
DNAat
af tn Ribonudto H ho emoVLd u KNA

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OKaialC
munfom
Derlvsd fY a nmo Phit
hemo.tahLr
Stub a

a
m
bactotium n2
hanConveno
Convekeu.
un knP

rm
t Muc
DNA ga

DNA kchno Loj APPLkon

b)tE Combinast
mdiun.
Recombinghon
ha DNA (TpNA) Technaloq
lP
Kecomblnant DNA technol9 oning
toqithchof DNA moltuks rauithdo dittoat
Iccombinsd DNA mocu
sped T
Produua
indenked ino a
hot 0Tonism
el

Jenhu combln.aiond that oUL Val s

udiun aaaituhandia ost
ew

O RecomblaantDNA GDNA) maltul an DNA

abuYador muthods ot enhe materiafrom
ar

mulip Soutes (raing Ssqunceh #hat

0hm u b2
C

Thn a mau ouvevuL and Comp Lax_ kchigus

Juvlyzd
Prinupl
ma maPulaHon
of NA tchnmlog
Kcn ab Sim?le h velves folO

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Poge

nenYaan of DNA
and Shtie of
of DNA
Iskroduuion f nmri ONA i hoE_CelW3
EX Backeia

a
Nulhpbcsakie Cund- LLonasfrr.

rm
TeCombinau Moukes.
ExpTon of toPro clu he cuu
rRduek

ha PlaAmid D»A
lP
Donch DNA-
Rom ion
Retri cisn edonudka
clonudce
el

Pcu
DeaidDNA
ew

Cut Ptashmid DNA

ar

Re combihaxt Dnf
Lrodu i
C

MuAPitation
Shcin of do

ot-en nloca d
qu
dod

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rerptei nginuning _dl

APPLca0n of

Callid aA uhtic moa

Cnentkic Enginuing also
maneulak nsofot
abitt

a
abl agius
PYOkIN oobtn1
0anismL roduc du
trat

rm
0anism tho hal au hubaaphliasi
runutic cninuring techg
EAearnc
_numLYous_ Hsincludiog
bltechnol midiun

0 PYodu chion
of
ha
banana Vacain

ehiA
Reseanhbein dons
bePtPut Succoaf
Canbe Succedfu
lP
oh Potedo cs Sho hat VaccinAA cgnat
Plants hat U maki
inhPotao B
chokra, diarha and hlaktis
el

i Tcatnt PihPass-
cheicenpuing ctiul inFSH
Produckion e huitm inac ction, iA Contoind
ew

hormob ufulo7 tYeahr ate iki*

Vaccinahon nfo
ar

mCa-aaandsm Lsuthss
scde
wwd utodPYocu Vru ankih
d a Vacdn tkSthmulakss human a
C

mmun Vir

+
u Gu HTalh ha bu Succotu
Todt_chroni mPh oy akeia (Cu) and
Pcnar on's da se an).

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echnslo
Cc)H APPLtakiOn
th
Poddhion sf
ndi9he
Cinlng-
(i ronON_ Vaccinu= h PakAs-G C
Inuferon Hoorw Thyudin

a
Intorferon- Into tersn aanivir substu

rm
irta frn ho 0insdtd from t-
intteYeh of macu n YJ IPLtokion_
0zn distov.eadinasi b ackisas
and Jcan ndemann and onbicund to

ha
Sing Subsctu det ontn
onsist>
Sbsta pedetton ui* muktukan lerahA
lP
bctwca 20,00 0 30, 000 d altons
inkenferons h av PoYkeNous htun
and map- af m anA lycofrokis
Lrttnfe YOnd brOaadly lake govhel int tnu
el

bard

Tterfenon lIENJ
ew

Lnttntenon-8 LIFN8]
Tkrfcnon -Y LTENY
ar

Patia B Vaccis-
HPad is
disesm huma bun It Ainan
C

Ayord
cffeuts adsto
Pattid UThAIS and
Hfais viYs ing of Sifanick
42 nm d (ud"da
Ponti Ponh (O IS H "Con"

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Coton ViYa genom (ONA) i

SOTTOunduos Phospho Lpid nveloPa
b

a
Scf V
Pah A B Vaccin R
donot PYo duc la i

rm
fhdvL ancd
Yrattie duu o tu as ons hAVacc
has b wn Sn co 987A0dale
Humas sulln-

ha
LAuni o hormoN WAd
A POdu tel
of Pancreas Thus human
lP
Contwn 54 amino
turo PokPePid dhainb.
T A &kain Consis Ofa k
amino auds etk tkea huld
el

toqtn disulfidi boncl

ew

O Diabke lks
Diahte mlliAuJ
abou atfs
23 of no PoPLukion Tt
a
iolol usca _
ar

darackai b incealsl
blmd Ths qluLa ws-
indutiuek_ mai oCCuod d
C

pyodu
DNA_
telo

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Bris
Itsdudion PR Pomart
dhain a isn-

a
Th PymiYk_Chaln a loborah
Yacton eck_
technigusf ax quantiksA ot a ckedna

rm
DNA obvisul PcR a Llfr amfliRcton
techniqu for SJibusiing muliP idntito_opies
oiliond An DNA intereNA

ha
nulla fPca
T
douba -stYanchd DNA of
ret is duhotund o
Scparale tun indi visuo
lP
SAYahd_ SATOndis han aoadto hbiu
Psimn (TenouYahion)
u SkA cunatuY sh ona Ychoturk a an
Sadeesis ebeoatol dnand \ agcan t JuY k
el

pmulhp frmd of tan.t DNA,

ew

ThaiqusOf PCR
T e ssehl YequmtA tr
PCRaw kdibelous
1A Atanmt DNA (100-35.00o bPingt)
ar

To xinn Sgnioigonucoics of_11-3o nules-

Ads ungh) Hht is_ (omPlamkary to eond tlanang-
ONA
C

3 Fon duoxtihonucleatids dAT dCTP, dhTedT

DNA Pohmro
9SC Le thume Satub&

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Acaki eua af pK
APP
C-PR loitat. dagnalA is

a
Sensit vi of Pc
speatiutand Vonious
cuagnssis of

rm
wtull
disea humau

andr daaanedsof hesited disasl i

diaanohA of

ha
Pc Pienat
emfloal da
inhiked disca
wn Choionte
amnuo untes's,
SnP EYom_
lP
(31 Diaanasof
Scwn Vir
Virl kudol anten LeCenvicek atar
Caudu
badukakdb
PaPiLlomas
PCRi
VYuA d
el

PR S dLtaninak ebyo
ew

Sex of hma
huma a LvA Stackembyoys
fnhd ViAYoViEYo Can b dutamnicd
b
wNng Primub and DNAProhos
speii tor
ar

Sex omo A0mis

C

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