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Research Plan
Research Plan
MOSQUITO
RESEARCHER PROPONENTS:
JOYELLE N. GALLARDO
JOSHUA N. LANTINGAN
JAYMAR QUITOR
PROJECT QUESTION:
Is Oregano leaves and African Talisay leave extracts effective as an alternative mosquito
repellent in helping us avoid and lower the case of Dengue?
INTRODUCTION
Dengue is one of the most alarming diseases that if faced globally and nationally.
Dengue is a mosquito-borne illness that occurs in tropical and subtropical areas of the world.
It has remained an endemic disease ever since the first epidemic in Southeast Asia was
documented in Manila 1954. The viral illness dengue, which is spread by mosquitoes, has
recently spread quickly throughout all WHO areas. Female mosquitoes, primarily of the
species Aedes aegypti and, to a lesser extent, Ae, carry the dengue virus. Albopictus. These
mosquitoes can transmit the Zika, yellow fever, and chikunguya viruses. The risk of dengue
varies locally depending on local climate, socioeconomic, and environmental factors, which
Dengue is endemic in more than 100 countries in Asia, the Pacific, the Americas,
Africa, and the Caribbean. Each year, up to 400 Million people get infected with dengue
globally. Up to 100 Million gets infected severely, and up to 40,00 people die from the said
disease. According to the World Health Organization (WHO), there were 4.2 million reported
cases of dengue in the Americas in 2019, with 1,489 deaths. In the Western Pacific region,
there were 1.2 million reported cases of dengue in 2019, with 1,254 deaths. Southeast Asia
has the highest burden of dengue in the world, with an estimated 67 million cases per year. In
our country, an increase of 191% from the 67,357 cases reported during the same period in
2021 to 196,728 cases and 642 deaths between 1 January and 5 November 2022(Department
of Health, 2022).
Currently, there is no treatment for dengue. But a vaccine was made that was
supposed to help prevent dengue from spreading. Dengvaxia was the name of the vaccine and
it was given to people ages 6 to 45 who had previously got the dengue virus (EUROPEAN
MEDICINES AGENCY, 2022). It was created by Sanofi Pasteur inc., a division of the
French pharmaceutical. They created the vaccine using recombinant DNA technology and
substituted homologous sequences from the four dengue virus serotypes with numerous
genetic regions found in the genome of a yellow fever vaccine virus (Centers for Disease
Control and Prevention). Dengvaxia was introduced in the Philippines, in April 2016. In
November 2017, more than 830,000 kids had at least one dose of the vaccine (Dengvaxia is
administered as three injections, with six-month intervals). In February 2018, the vaccination
had become a national scandal. Rumors says that Dengvaxia was linked to the deaths of
children. Many parents of children who died blamed the vaccine. Most of the deaths were
caused by internal bleeding in the heart, lungs, and brain, which are symptoms of
for dengue when in 2018, Sanofi Pasteur admitted that dengvaxia may increase the risk of
severe dengue in those who has no dengue infection before they were vaccinated
(Inquirer.net, 2019). Dengvaxia vaccine will only allow people who had been exposed to
dengue virus before to take it, but why make a prevention to dengue when it can only be
A considerable amount of research of the plant Oregano and African Talisay will
show us it’s efficacy on preventing the spread of dengue cases here in our country and
globally. Oregano is a plant that contains thymol, carvacrol, 1,8-cineole and p-cymene
substance (Science Direct, 2016), while African Talisay has tannins, saponins, Flavonoids,
and Terpenoids which is toxic for insects (Farrah, 2019). Different kinds of test will also be
conducted to show the efficacy of Oregano and African Talisay Leaves as mosquito repellent.
Test will also help us prove its safeness to people who already experienced dengue and to
people who did not experience the said disease before using it.
Hypothesis
HO: There is no significant effect on using Mosquito Repellent Spray made from Oregano
H1: There is a significant effect on using Mosquito Repellent Spray made from
Procedure
a. Pour half a cup of distilled water with 10pcs.Oregano and 1/8kilo of African
Talisay leaves into a sauce pan, then boil, to extract the leaves’ sap.
b. Chop the remaining oregano and African Talisay leaves to be put on the spray
c. If the water with oregano leaves and African Talisay leaves have boiled,
transfer the water into the spray bottles and add the chopped leaves into it.
d. Using the boiled leaves, extract the oil of the leaves and put it on the spray
e. Mix thoroughly the extracted oil of the leaves with the boiled distilled water.
5. Spray the finish product and observe the mosquito after spraying the repellent
6. Test the finish product to the laboratory before using it.
Materials
1kl of nails
(measurement of net)
Mosquito wrigglers
Data List
Risk Assessment
pesticide on human skin may lead to irritation and dermatitis (Environ Med, 2001). Making
sure that this pesticide should only be use on mosquitoes and not for other animals is a must.
Upon conducting this study, we will strictly follow a proper and safe way to perform this
Bibliography