R 15

Code No: A25E1

BVRAJU INSTITUTE OF TECHNOLOGY, NARSAPUR
(UGC - AUTONOMOUS)
B.Tech III Year I Semester Supplementary Examinations, May/June 2019
BIOCHEMICAL ENGINEERING
(Chemical Engineering)
Time: 3 Hours Max Marks: 70
Note: This Question Paper contains two Parts A and B
Part A is compulsory which carries 20 marks. Answer all questions in Part A at one place only.
Part B Consists of 10 questions. Answer any five (one full question from each unit). Each question
carries10 marks and may have a, b, c as sub questions.

PART – A (10x2 = 20 Marks)

1. a) What are Procaryotes? Give two examples?
b) Write a short note on important cell types?
c) Discuss the effect of external mass transfer resistance on enzyme kinetics?
d) Write in brief about utilization and regeneration of cofactors?
e) What are the end products of metabolism?
f) What are the factors that affect the media formulation?
g) Discuss the scaling of mass transfer equipment?
h) How do you determine oxygen transfer rates?
i) What is electrophoresis?
j) Discuss the strategies to recover and purify products?
PART – B (5x10 = 50 Marks)
2. Discuss in detail the kinetics of enzyme catalyzed reaction?
OR
3. Describe DNA and RNA in detail?
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4. Explain physical entrapment for enzyme immobilization?
OR
5. Explain in detail about the different models for cellular growth?
***
6. Explain TCA cycle with neat sketch?
OR
7. Explain the transport processes across the cell membrane?
***
8. Discuss in detail about power requirement of agitated vessels under non aerated and aerated
conditions?
OR
9. With the help of schematic diagram explain the steps involved in the transport of oxygen from a
gas bubble to inside of the cell?
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10. Discuss indetail about the following solid-liquid separation processes
a)Filtration b) Centrifugation
OR
11. Explain in detail about different membrane separation methods?
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