SUPPLEMENTARY MATERIAL

Figure S-1. Adsorption kinetics of DXI-NPs at air/water interface at 32 C (n=3).

15

ECMM
10
ACMM
  (mN·m )
-1

0
10 20 30

-5
 i (mN m -1 )

Figure S-2. Surface pressure increase () as a function of initial lipid pressure due to DXI-
NPs action on ACMM or ECMM at 32 C. Solid lines correspond to the fit of experimental data
by lineal regression. Subphase: Tris-HCl buffer pH 7.4; [DXI-NPs]= 3.6x10-2g ml-1 (n=3).
Figure S-3. -A curves for (left) ACMM and (right) for ECMM at two different [DXI-NPs] at
the subphase (Tris-HCl buffer pH 7.4) (n=3).
 0.001
9.5 µg/mL
Fluorescence difference
23.7 µg/mL
0.000 47.5 µg/mL
350 400 450 500 550 600 95.0 µg/mL
142.5 µg/mL
-0.001 190.0 µg/mL
237.5 µg/mL

-0.002 /nm

0.0010
Fluorescence difference

9.5 g/mL
0.0005 23.7 g/mL
47.5 g/mL
0.0000
350 400 450 500 550 600 95.0 µg/mL
-0.0005 142.5 µg/mL
190.0 µg/mL
-0.0010 237.5 g/mL
/nm
-0.0015

Figure S-4. The normalized ratio (Rnorm) of di-8-ANEPPS fluorescence excitation spectra at
different DXI-NPs concentration (top) ACMM and (bottom) ECMM. The lipid concentration
was 400 µM. The experiments were performed at 32ºC.

Video-1. Deconvoluted video of the effect of GUVs of ACMM composition (red) after
30 min of incubation with DXI-NPs (green) at 32 C.