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Lecture 10 Vibrio, Aeromonas, Campylobacter and Helicobacter
Lecture 10 Vibrio, Aeromonas, Campylobacter and Helicobacter
Lecture 10 Vibrio, Aeromonas, Campylobacter and Helicobacter
V. mimicus, V. V. cholerae, V. Can grow in a media not containing salt (same with Pleisomonas)
parahaemolyticus, P. alginolyticus, V. fluvialis, V.
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Campylobacter and Helicobacter Broth with 20% glycerol and
frozen at −70° C
Most clinically relevant species: Commonly used selective media for
1. C. jejuni subsp. jejuni C. jejuni – Campy-BAP (blood agar
2. C. coli
plate)
3. C. fetus subsp. fetus
o Other media used:
Campylobacter Butzler medium and
Skirrow’s medium
GBS – Guillain-Barré Syndrome (1 in 1,000 diagnosed Culture Media
campylobacter infections) Commonly used media for H.
C. jejuni – most common cause of bacterial gastroenteritis pylori – CAP or Brucella agar with
worldwide 5% Horse red blood cells
o Self-limited (2-6 days) (nonselective medium)
o Some is sexually transmitted disease o Selective media:
o Diarrheal disease begins with mild abdominal pain within Skirrow’s agar
2-10 days after ingestion of the organisms. o Microaerophilic
Fever, chills, nausea and vomiting. environment
Mode of transmission:
o Direct contact with animals and handling infected pets,
such as dogs, cats, and birds
o Indirectly by the consumption of contaminated water and
dairy products and improperly cooked poultry
o Person-person transmission
Helicobacter
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o Stain poorly on gram-
stained smears
Carbol fuchsin
is recommended
as a counter
stain
o “Darting” motility
(corkscrew movement) on
hanging drop preparations
or when visualized under
phase contrast microscopy
o Single polar flagellum
Helicobacter spp. –
morphologically similar with
Campylobacter
o Curved or U-shaped
o Motile with multiple flagella
at one pole
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