AGRITECH, Vol. 36, No.

1, Februari 2016

POTENSI PROBIOTIK INDIGENUS Lactobacillus plantarum Dad 13 PADA YOGURT

DENGAN SUPLEMENTASI EKSTRAK UBI JALAR UNGU UNTUK PENURUN DIARE
DAN RADIKAL BEBAS
3RWHQWLDO3URELRWLFLactobacillus plantarum,QGLJHQRXV'DGRQ<RJKXUWZLWK3XUSOH6ZHHW3RWDWR([WUDFW
6XSSOHPHQWDWLRQWR5HGXFLQJ'LDUUKHDDQG)UHH5DGLFDOV

Agustina Intan Niken Tari, Catur Budi Handayani, Sudarmi

3URJUDP6WXGL7HNQRORJL+DVLO3HUWDQLDQ8QLYHUVLWDV9HWHUDQ%DQJXQ1XVDQWDUD
-O/HWMHQ6+XPDUGDQL1R-RPERU6XNRKDUMR
Email : intanniken@gmail.com

ABSTRAK

3HQHOLWLDQ EHUWXMXDQ PHPSHODMDUL HIHNWLYLWDV VWUDLQ SURELRWLN LQGLJHQXV WHUSLOLK Lactobacillus plantarum Dad 13)
SDGD \RJXUW GHQJDQ VXSOHPHQWDVL HNVWUDN XEL MDODU XQJX VHEDJDL SHQXUXQ GLDUH GDQ UDGLNDO EHEDV SDGD WLNXV SXWLK
DOELQR 1RUZD\ UDWV Rattus novergicus) galur Sprague dawley. Penelitian dirancang menggunakan rancangan acak
OHQJNDSIDNWRULDOGHQJDQSHUODNXDQ\RJXUWHNVWUDNXELMDODUXQJXWDQSDSURELRWLN3\RJXUWHNVWUDNXELMDODUXQJX
GHQJDQSURELRWLN3WHUKDGDSNHORPSRNWLNXVSprague dawleyMDQWDQ\DQJGLEHULSHUODNXDQWDQSDHQWHURSDWKRJHQLN
Escherichia coli (3(&$7&&(PDXSXQGHQJDQHQWHURSDWKRJHQLNEscherichia coli(3(&$7&&
(3HPEHULDQSURELRWLNGLODNXNDQGHQJDQPHWRGHVRQGHSDGDKDULNHVDPSDLGHQJDQGRVLVPOJ%%
WLNXVSHUFREDDQDWDXUDWDUDWD9&)8PO6HGDQJNDQSHUODNXDQ(3(&$7&&GLODNXNDQGHQJDQPHWRGHVRQGH
VHEDQ\DN6&)8POSDGDKDULNHVDPSDLNH3DUDPHWHU\DQJGLDPDWLPHOLSXWLNDGDUDLUIHVHVNDGDUDLUVHNXP
NDGDUPDORQDOGHKLGH0'$GDUDKGDQKDWL+DVLOSHQHOLWLDQPHQXQMXNNDQEDKZD7HUGDSDWLQWHUDNVLSHUODNXDQ
DQWDUD SHPEHULDQ (3(&$7&&  GDQ SURELRWLN LQGLJHQRXV SDGD \RJXUW HNVWUDN XEL MDODU XQJX WHUKDGDS NDGDU
DLUIHVHVNDGDUDLUVHNXPNDGDU0'$GDUDKGDQNDGDU0'$KDWLWLNXVXML.XOWXULactobacillus plantarum Dad
\DQJGLWDPEDKNDQSDGD\RJXUWHNVWUDNXELMDODUXQJXPDPSXPHPEHULHIHNNHVHKDWDQVHEDJDLSHQXUXQGLDUHGDQ
radikal bebas.

Kata kunci3HQFHJDKGLDUHSHQXUXQUDGLDNDOEHEDV\RJXUWHNVWUDNXELMDODUXQJXEDNWHULSURELRWLN

ABSTRACT

7KHSXUSRVHRIWKLVVWXG\ZDVVWXG\LQJWKHHIIHFWLYHQHVVRIVHOHFWHGLQGLJHQRXVSURELRWLFVWUDLQVLactobacillus plantarum

'DGLQ\RJKXUWZLWKSXUSOHVZHHWSRWDWRH[WUDFWVXSOHPHQWDWLRQDVUHGXFLQJGLDUUKHDDQGIUHHUDGLFDOVRQZKLWH
UDWVDOELQR1RUZD\UDWVRattus novergicus) Sprague dawleyVWUDLQ7KHVWXG\ZDVGHVLJQHGXVLQJIDFWRULDOFRPSOHWHO\
UDQGRPL]HGGHVLJQZLWKWUHDWPHQWRISXUSOHVZHHWSRWDWRH[WUDFW\RJXUWZLWKRXWSURELRWLFV3SXUSOHVZHHWSRWDWR
H[WUDFW \RJXUW ZLWK SURELRWLF 3 WR  JURXSV RI PDOH Sprague dawley UDWV ZHUH WUHDWHG ZLWKRXW (QWHURSDWKRJHQLN
Escherichia coli(3(&$7&&(DQGZLWK(QWHURSDWKRJHQLNEscherichia coli(3(&$7&&(
3URELRWLFWUHDWPHQWZDVFRQGXFWHGXVLQJWKHVRQGHDWGD\st to 21stDWDGRVHRIPOJZHLJKWRUDYHUDJH9&)8
PO:KLOHWKHWUHDWPHQWRI(3(&$7&&ZDVFRQGXFWHGXVLQJWKHVRQGHDWGRVHRI6&)8PORQGD\thWRth.
7KHREVHUYHGSDUDPHWHUVLQFOXGHIHFDOZDWHUFRQWHQWZDWHUFRQWHQWRIFHFXPPDORQDOGHKLGHOHYHOV0'$EORRGDQG
liver.7KHUHVXOWVVKRZHGWKDW7KHUHZDVLQWHUDFWLRQEHWZHHQWKHWUHDWPHQWRILQGLJHQRXVSURELRWLF\RJXUWSXUSOH
VZHHWSRWDWRH[WUDFWDQG(3(&$7&&RQZDWHUFRQWHQWRIIDHFDOZDWHUFRQWHQWRIFHFXP0'$OHYHOVEORRG
DQGOLYHU&XOWXUHRILactobacillus plantarum'DGZDVDEOHWRSURYLGHKHDOWKHIIHFWVDVUHGXFLQJRIGLDUUKHDDQG
free radicals.

Keywords5HGXFLQJRIGLDUUKHDIUHHUDGLFDOVSXUSOHVZHHWSRWDWRH[WUDFW\RJXUWSURELRWLFEDFWHULD

7
 AGRITECH, Vol. 36, No. 1, Februari 2016

PENDAHULUAN \RJKXUW GDSDW PHQLQJNDWNDQ DNWLYLWDV DQWLRNVLGDQ VHKLQJJD

EHUSRWHQVLPHQJXUDQJLNDGDUOLSLGGDODPGDUDK
3URELRWLN DGDODK PLNURRUJDQLVPH KLGXS \DQJ VDDW 3HQLQJNDWDQ NKDVLDW XEL VHKLQJJD GDSDW GLPDQIDDWNDQ
GLNRQVXPVL GHQJDQ MXPODK \DQJ FXNXS WHWDS KLGXS VDPSDL VHFDUD PDNVLPDO VHEDJDL GLHW DOWHUQDWLI SHUOX GLODNXNDQ
VDOXUDQ SHQFHUQDDQ VHUWD PHPEHULNDQ PDQIDDW NHVHKDWDQ NDUHQD PDV\DUDNDW PDVLK PHPDQGDQJ XEL MDODU VHEDJDL
bagi tubuh melalui keseimbangan mikrobiota )$2  PDNDQDQNHODVEDZDK6DODKVDWXEHQWXNLQRYDVLSHQJRODKDQ
0HQXUXW 3DUYH] GNN  EDNWHUL SURELRWLN EHUPDQIDDW PDNDQDQDOWHUQDWLIGDULXELMDODUXQJXDGDODK\RJXUW
PHQLQJNDWNDQVLVWHPNHNHEDODQWXEXKGDQPHPSXQ\DLHIHN
NHVHKDWDQ VHSHUWL PHQJXUDQJL NHMDGLDQ lactose intolerance,
PHQFHJDK KLSHUWHQVL GDQ VHEDJDL SHQFHJDK GDQ PHPSXQ\DL
HIHN WHUDSHXWLN PHODZDQ GLDUH 3URELRWLN MXJD GLODSRUNDQ
PDPSX PHQJDWDVL GLDUH \DQJ GLVHEDENDQ ROHK E.coli, baik
7DUL GNN  PHODSRUNDQ EDKZD \RJXUW GHQJDQ
SHQDPEDKDQ HNVWUDN XEL MDODU XQJX PHQJJXQDNDQ NXOWXU
NRPHUVLDO GDQ SURELRWLN LQGLJHQXV \DLWX Streptococcus
thermophillus )1&& , Lactobacillus bulgaricus
)1&& dan Lactobacillus plantarum Dad 13 dengan
enterotoksigenik E.coli (7(& 2HWD\R  PDXSXQ SHUEDQGLQJDQ  PHUXSDNDQ \RJXUW WHUEDLN GLDQWDUD
enterohemorargi E. coli(+(&0HGHOOLQ3HQDGDQ*ULI¿WKV SHUODNXDQSHUODNXDQ SHQJJXQDDQ NXOWXU NRPHUVLDO
(IHNWHUVHEXWPXQFXOMLNDMXPODKEDNWHULKLGXSVDPSDL GDQ SURELRWLN LQGLJHQXV ODLQQ\D NXOWXU Streptococcus
GL VDOXUDQ SHQFHUQDDQ OHELK GDUL 6 &)8J DWDX 6&)8 thermophillus )1&&  Lactobacillus bulgaricus
PO 2\HWD\R  3HQHOLWLDQ WHUGDKXOX PHPEXNWLNDQ
bahwa secara in vitro bakteri galur Lactobacillus dan
%L¿GREDFWHULD GDSDW PHQJKDPEDW SHQHPSHODQ GDQ LQYDVL
EDNWHUL HQWHURSDWKRJHQ SHQ\HEDE GLDUH %RXUOLRX[ GNN
)1&& GDQ SURELRWLN Lactobacillus plantarum Mut
 GHQJDQ SHUEDQGLQJDQ  GDQ NXOWXU Streptococcus
thermophillus )1&& , Lactobacillus bulgaricus
)1&& GDQ SURELRWLN Lactobacillus acidophilus 613
 3HUV\DUDWDQ MXPODK VHO KLGXS SURELRWLN GDODP VXVX
 GHQJDQ SHUEDQGLQJDQ  <RJXUW WHUVHEXW PHPLOLNL
fermentasi minimal 107&)8J&RGH[\DQJ GLKDUDSNDQ
VLIDW¿VLNS+ YLVNRVLWDV F3ZDUQDNURPDWLN
GDSDW PHQJDQWLVLSDVL SHQXUXQDQ MXPODK VHO VHODPD PHOHZDWL
 VLIDW NLPLD NDGDU DVDP WHUWLWUDVL  NDGDU
OLQJNXQJDQHNVWUHPGLSHQFHUQDDQ6KDKdkk.%HEHUDSD
DLU    NDGDU DEX  NDGDU JXOD UHGXNVL
SHQHOLWLMXJDPHODSRUNDQEDKZDPHQJNRQVXPVLEDNWHULDVDP
NDGDUSURWHLQWHUODUXWNDGDUOHPDN
ODNWDW %$/ JRORQJDQ Lactobacillus PDPSX PHQLQJNDWNDQ
GDQ NDGDU DQWRVLDQLQ  VLIDW RUJDQROHSWLN GHQJDQ
VLVWHPLPXQVHOXOHUGDQKXPRUDO*DFNRZVNDGNN
VNRUNHQDPSDNDQUDVDGDQDURPDGDULVNDOD
%HEHUDSD isolat lokal EDNWHUL DVDP ODNWDW \DQJ WHODK
PDNVLPDO9LDELOLWDV%$/VHODPDGXDPLQJJXSHQ\LPSDQDQ
EHUKDVLOGLLVRODVLGDQPHPSXQ\DLVLIDWSURELRWLNDQWDUDODLQ
109 &)8PO SDGD VXKX o& GDQ DNWLYLWDV SHQFHJDK GLDUH
Lactobacillus plantarum0XW\DQJGLLVRODVLGDULPDNDQDQ
GHQJDQ NHPDPSXDQ PHQXUXQNDQ HQWHURSDWRJHQLF E.coli
IHUPHQWDVLNHWHODJDWRW5DKD\XGNNLactobacillus
$7&&  VDPSDL  ORJ cycle selama dua minggu
plantarum'DG\DQJGLLVRODVLGDULVXVXIHUPHQWDVLNHUEDX
SHQ\LPSDQDQSDGDVXKXo&VHFDUDin vitro.
GDGLK1JDWLUDKGNNVHUWDLactobacillus acidophilus
.HPDPSXDQNXOWXUNRPHUVLDOGDQSURELRWLNLQGLJHQXV
613 \DQJ GLVRODVL GDUL IHVHV ED\L \DQJ PHQJNRQVXPVL
\DLWX Streptococcus thermophillus )1&&, Lactobacillus
DLU VXVX LEX $6, 3XUZDQGDQL GDQ 5DKD\X  8QWXN
PHPSHUWDKDQNDQ KRPHRVWDWLV NRORQL PLNURÀRUD VDOXUDQ
SHQFHUQDDQ GLSHUOXNDQ DGDQ\D QXWULHQ \DQJ PDVXN NH
GDODP XVXV .RORQLVDVL ROHK SURELRWLN XQWXN PHPEHQWXN
PLNURHNRVLVWHP \DQJ QRUPDO GDSDW GLPDQLSXODVL PHODOXL
SHQJDWXUDQ GLHW \DQJ PHQJDQGXQJ SUHELRWLN SURELRWLN DWDX
NRPELQDVL NHGXDQ\D \DQJ GLVHEXW VLQELRWLN 5DVWDOO GDQ
bulgaricus )1&& dan Lactobacillus plantarum Dad
 GHQJDQ SHUEDQGLQJDQ  SDGD \RJXUW GHQJDQ
SHQDPEDKDQ HNVWUDN XEL MDODU XQJX VHEDJDL SHQXUXQ GLDUH
dan radikal bebas belum diketahui. 8QWXN LWX PDND SDGD
SHQHOLWLDQ LQL GLODNXNDQ VHFDUD in vivo menggunakan tikus
SHUFREDDQ +LSRWHVLV GDUL SHQHOLWLDQ LQL DGDODK EDKZD %$/
*LEVRQ Lactobacillus plantarum Dad 13 \DQJ GLVXSOHPHQWDVLNDQ
3DUD DKOL VDDW LQL VHGDQJ JHQFDU PHODQFDUNDQ NRQVHS SDGD\RJXUWGHQJDQSHQDPEDKDQHNVWUDNXELMDODUXQJXGDSDW
“food as medicine.” .RQVHSWHUVHEXWPHUXSDNDQVHEXDKFDUD PHQXUXQNDQ GLDUH \DQJ GLVHEDENDQ ROHK Enteropathogenic
XQWXN PHQJRSWLPDONDQ SDQJDQ IXQJVLRQDO XQWXN PHPEDQWX Escheirchia coli (3(& $7&&  GDQ UDGLNDO EHEDV
PHQJDWDVL SHQ\DNLW 6DODK VDWX EDKDQ SDQJDQ IXQJVLRQDO \DQJGLLQGLNDVLNDQGHQJDQPHQXUXQQ\Dkadar malonaldehida
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PHQJDQGXQJ DQWLRNVLGDQ VHSHUWL DVDP SKHQRODW DQWRVLDQLQ $GDSXQ WXMXDQ SHQHOLWLDQ LQL DGDODK PHPSHODMDUL
GDQ  WRNRIHURO \DQJ GDSDW PHQFHJDK WLPEXOQ\D EHEHUDSD HIHNWLYLWDV VWUDLQ SURELRWLN LQGLJHQXV WHUSLOLK Lactobacillus
SHQ\DNLW :RROIH  +DVLO SHQHOLWLDQ 5HWQDWL  plantarum DadSDGD\RJXUWGHQJDQVXSOHPHQWDVLHNVWUDN
PHQXQMXNNDQ EDKZD SURVHV IHUPHQWDVL SDGD SHPEXatan XELMDODUXQJXVHEDJDLSHQXUXQGLDUHGDQUDGLNDOEHEDVSDGD

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 AGRITECH, Vol. 36, No. 1, Februari 2016

WLNXV SXWLK DOELQR 1RUZD\ UDWV Rattus novergicus) galur Pembuatan Yogurt dengan Suplementasi Ekstrak Ubi
Sprague dawley. Jalar Ungu
6XVX VHJDU  PO VXVX VNLP  EY GDQ HNVWUDN
METODE PENELITIAN XEL MDODU XQJX  YY GLSDVWHXULVDVL SDGD VXKX o&
VHODPD  PHQLW NHPXGLDQ GLGLQJLQNDQ VDPSDL VXKX 
Tikus Sprague Dawley Jantan
Pakan standar o& 6HODQMXWQ\D GLLQRNXODVL PHQJJXQDNDQ Streptococcus
thermophillus dan Lactobacillus bulgaricus serta bakteri
$GDSWDVL minggu
SURELRWLN indigenus Lactobacillus plantarum Dad 13 dengan
Pakan VHVXDLSHUODNXDQ
selama 21 hari, SHUEDQGLQJDQ\DQJGLODNXNDQVHFDUDDVHSWLVSDGD
VXKX o& VHEDQ\DN   YY NHPXGLDQ GLNRFRN
<(873 3 <(8'3 3 <(873 3 <(8'3 3 hingga homogen.6XVXGDQHNVWUDNXELMDODUXQJX\DQJWHODK
diinokulasi dengan starter dimasukkan ke dalam botol-botol
7DQSD3HPEHULDQ(3(& ( 'HQJDQ3HPEHULDQ(3(&( VWHULOGDQGLLQNXEDVLSDGDVXKXo&VHODPDMDPKLQJJD
Pembedahan
 hari ke 21 GLKDVLONDQ\RJXUWHNVWUDNXELMDODUXQJX

Analisis kadar air feses dan sekum Pengelolaan Hewan Coba

Analisis kadar MDA dalam darah dan hati
Gambar 1. Uji in vivoEDNWHULSURELRWLNSDGD\RJXUWHNVWUDNXELMDODU
XQJX GDQ HIHNWLYLWDVQ\D VHEDJDL SHQFHJDK GLDUH GDQ
SHQXUXQUDGLNDOEHEDV
Keterangan:
Penelitian ini dilakukan dengan rancangan acak
NHORPSRN OHQJNDS SROD )DNWRULDO PHQJJXQDNDQ KHZDQ
FRED EHUXSD WLNXV MDQWDQ Sprague Dawley dengan umur 2
EXODQKDVLOSHQJHPEDQJELDNDQGDUL%3205,GHQJDQERERW
EDGDQJ.DQGDQJ\DQJGLJXQDNDQDGDODKNDQGDQJ
<(8733 <RJXUW GHQJDQ HNVWUDN XEL MDODU XQJX WDQSD SHPEHULDQ LQGLYLGX \DQJ EHUXNXUDQ  [  [  6XKX UXDQJDQ
WDPEDKDQSURELRWLNNRQWURO GLDWXU SDGD o& 0XFKWDGL  5DQVXP GLEHULNDQ
<(8'33 <RJXUW GHQJDQ HNVWUDN XEL MDODU XQJX GDQ SHPEHULDQ VHEDQ\DN  J SHU HNRU SHU KDUL VHWLDS SXNXO 
WDPEDKDQSURELRWLN
(  7DQSDSHUODNXDQHQWHURSDWKRJHQLFEscherichia coli(3(& :,%$LU PLQXP GLEHULNDQ VHFDUD ad libitum 6HWLDS  KDUL
$7&& GLODNXNDQSHQLPEDQJDQERERWEDGDQGDQSHQFXFLDQNDQGDQJ
(  'HQJDQ SHUODNXDQ HQWHURSDWKRJHQLF Escherichia coli .RPSRVLVL UDQVXP EDVDO GLVXVXQ EHUGDVDUNDQ VWDQGDUW
(3(&$7&&
$,1American Institute of Nutrition\DQJWHUGLULGDUL
Pembuatan Ekstrak Ubi Jalar Ungu WHSXQJMDJXQJNDVHLQVXNURVDPLQ\DN
NHGHODL  VHOXORVH  PLQHUDO  YLWDPLQ 
3HPEXDWDQVDULXELMDODUXQJXPHQJDFXSDGD7DUL /VLVWLQ  NROLQELWDUWUDW  GDQ 7%+4 Tert-
\DLWX XEL MDODU GLSRWRQJ NHFLO XNXUDQ  [  FP NHPXGLDQ
dimasukkan ke dalam juicer. Produk didiamkan 30 menit
VDPSDLMDPSDGDVXKXo&XQWXNPHQJHQGDSNDQSDWLQ\D
NHPXGLDQGLDPELO¿OWUDWQ\D)LOWUDWLQLPHUXSDNDQHNVWUDNXEL
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POPHGLXP056EURWKVWHULOVHEDQ\DNWDEXQJNHPXGLDQ
masing-masing tabung diinokulasi dengan biakan tegak
Lactobacillus bulgaricus )1&&  Streptococcus
thermophilus )1&&  Lactobacillus plantarum Dad
13. .HVHPXD LVRODW GLLQNXEDVL SDGD VXKX o& VHODPD
 MDP 3HPEXDWDQ NXOWXU VWDUWHU GLODNXNDQ GHQJDQ FDUD
PHPLSHW PDVLQJPDVLQJ  PO ELDNDQ WHUVHEXW NHPXGLDQ
butylhydroquinon
Perlakuan terhadap Hewan Percobaan dan Pengambilan
Sampel
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SHQHOLWLDQ VHPXD WLNXV GLEHUL UDQVXP VWDQGDU 3DGD PLQJJX
SHUWDPD SHQHOLWLDQ VHPXD WLNXV SHUFREDDQ GLEHUL SDNDQ
VWDQGDUWVHEDJDLDGDSWDVL3HPEHULDQ\RJXUWHNVWUDNXELMDODU
XQJX GHQJDQ SURELRLN PDXSXQ WDQSD SURELRWLN GLODNXNDQ
VHODPDPLQJJXSHQXK\DLWXSDGDKDULNHVDPSDLKDULNH
 VHWHODK PDVD DGDSWDVL VHFDUD RUDO PHQJJXQDNDQ VRQGH
GDQGLEDUHQJLSHPEHULDQSDNDQVWDQGDUW3RSXODVL%$/SDGD
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PO VHWLDS HNRU WLNXV ,QIHNVL (3(&$7&&  GLODNXNDQ
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menginokulasikan ke dalam 5 ml susu skim steril, dan SHUKDULVHODPDKDULKDULNHVDPSDLNHVHFDUDRUDO
PHQJLQNXEDVLQ\DSDGDVXKXo&VHODPDMDPDWDXSDGD menggunakan sonde. Pembedahan tikus untuk mengetahui
suhu 36o&VHODPDMDP NDGDU DLU VHNXP NDGDU 0'$ 0DORQDOGHKHKLGH GDUDK GDQ
KDWLWLNXVGLODNXNDQSDGDKDULNH

9
 AGRITECH, Vol. 36, No. 1, Februari 2016

Pengamatan terhadap Terjadinya Diare pada Tikus 70

Terinfeksi Enteropathogenic Escherichia Coli (EPEC) 60 E1P0 : E1P1 :
ATCC 35218 63,3229 62,7257

.DGDUDLU
50
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PHQJXNXU NDGDU DLU IHVHV \DQJ GLNROHNVL SDGD KDUL NH 
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DLU IHVHV GDQ VHNXP PHQJLNXWL SURVHGXU DQDOLVLV NDGDU DLU 20 
PHQXUXW$2$& E0P0
10 :17,6857
Pemeriksaan Kadar MDA 0
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dilakukan dengan metode Thiobarbituric acid substances
.HWHUDQJDQ(R WDQSDE.coli( GHQJDQE.coli
7%$56GLODNXNDQGL/DERUDWRULXP*L]L3$83DQJDQGDQ
3R WDQSDSURELRWLN3 GHQJDQ3URELRWLN
*L]L8*0<RJ\DNDUWD3HPHULNVDDQ0'$PHQJLNXWLPHWRGH
&DSH\URQ GNN  1LODL 7%$56 GLQ\DWDNDQ GDODP
*DPEDU3HQJDUXK LQWHUDNVL SHQDPEDKDQ SURELRWLN SDGD \RJXUW
PPROO6HGDQJNDQQLODL7%$56KDWLGLQ\DWDNDQGDODPQPRO
GDQSDSDUDQHQWHURSDWKRJHQLNEscherichia coli(3(&
0'$JMDULQJDQKDWL $7&&SDGDWLNXVXMLWHUKDGDSNDGDUDLUIHVHV
Analisis Statistik
*DPEDU  MXJD PHPSHUOLKDWNDQ EDKZD DGDQ\D
'DWD \DQJ GLSHUROHK SDGD SHQHOLWLDQ GLDQDOLVLV SHUODNXDQ SDSDUDQ (3(& $7&&  PHQ\HEDENDQ
menggunakan uji RAL Faktorial–Anova -LND SHUODNXDQ WHUMDGLQ\DSHQLQJNDWDQNDGDUDLUIHVHVEDLNSDGDNHORPSRN
PHQXQMXNNDQSHQJDUXK\DQJQ\DWDPDNDGLODQMXWNDQGHQJDQ WLNXV (,3 PDXSXQ (3 GHQJDQ UDWDUDWD NDGDU DLU GLDWDV
uji Duncan. \DQJPHQDQGDNDQNHMDGLDQGLDUH.HORPSRNWLNXVXML
(3 ZDODXSXQ PHQJDODPL NHMDGLDQ GLDUH QDPXQ NDGDU
HASIL DAN PEMBAHASAN DLU VHGLNLW OHELK UHQGDK GDULSDGD NHORPSRN WLNXV XML (3
+DO LQL GLPXQJNLQNDQ NDUHQD DGDQ\D SURELRWLN SDGD \RJXUW
Kadar Air Feses Hewan Sesudah 1 Minggu Perlakuan HNVWUDNXELXQJXPDPSXPHODNXNDQPHNDQLVPHSHUOLQGXQJDQ
dengan EPEC ATCC 35218 WHUKDGDSEDNWHUL(3(&$7&&0HQXUXW&ROODGRGNN
+DVLO DQDOLVLV 6366 PHQJJXQDNDQ $QRYD  PHNDQLVPH SHUOLQGXQJDQ EDNWHUL SURELRWLN WHUKDGDS
PHPSHUOLKDWNDQ EDKZD DGD LQWHUDNVL Q\DWD S DQWDUD
SHUODNXDQ HQWHURSDWKRJHQLF E.coli DWDX (3(&$7&& 
GDQSHPEHULDQ\RJXUWGHQJDQSURELRWLNLQGLJHQRXVWHUKDGDS
NDGDU DLU IHVHV SDGD WLNXV XML ,QWHUDNVL DQWDU SHUODNXDQ
WHUKDGDSNDGDUDLUIHVHVGDSDWGLOLKDWSDGD*DPEDU
*DPEDUPHQXQMXNNDQIHQRPHQDEDKZDWDQSDSDSDUDQ
EDNWHUL SDWKRJHQ DQWDUD ODLQ PHODOXL NRPSHWLVL SHQHPSHODQ
SDGD VLVL LNDWDQ GDQ QXWULHQW PHPRGXODVL VLVWHP LPXQ GDQ
VHNUHVLVHQ\DZDDQWLPLNURELD3DGD*DPEDUWHUOLKDWEDKZD
VHOLVLK NDGDU DLU IHVHV NHORPSRN (3 GHQJDQ (3 VDQJDW
NHFLOKDOLQLGLPXQJNLQNDQNDUHQDPHNDQLVPHSHUOLQGXQJDQ
SURELRWLN\DQJWHUNDQGXQJSDGD\RJXUWHNVWUDNXELMDODUXQJX
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