Chapter 1. The Study of Life

Biology for AP® Courses
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JOHN EGGEBRECHT, BROOKLYN TECHNICAL HIGH SCHOOL

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Table of Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Unit 1
Chapter 1: The Study of Life . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.1 The Science of Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.2 Themes and Concepts of Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Chapter 2: The Chemical Foundation of Life . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
2.1 Atoms, Isotopes, Ions, and Molecules: The Building Blocks . . . . . . . . . . . . . . . 42
2.2 Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
2.3 Carbon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Chapter 3: Biological Macromolecules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
3.1 Synthesis of Biological Macromolecules . . . . . . . . . . . . . . . . . . . . . . . . . 88
3.2 Carbohydrates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
3.3 Lipids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
3.4 Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
3.5 Nucleic Acids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Unit 2
Chapter 4: Cell Structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147
4.1 Studying Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
4.2 Prokaryotic Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
4.3 Eukaryotic Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
4.4 The Endomembrane System and Proteins . . . . . . . . . . . . . . . . . . . . . . . . 166
4.5 Cytoskeleton . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173
4.6 Connections between Cells and Cellular Activities . . . . . . . . . . . . . . . . . . . . 178
Chapter 5: Structure and Function of Plasma Membranes . . . . . . . . . . . . . . . . . . . 195
5.1 Components and Structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
5.2 Passive Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 204
5.3 Active Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 212
5.4 Bulk Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 218
Chapter 6: Metabolism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 237
6.1 Energy and Metabolism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 238
6.2 Potential, Kinetic, Free, and Activation Energy . . . . . . . . . . . . . . . . . . . . . . 242
6.3 The Laws of Thermodynamics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 252
6.4 ATP: Adenosine Triphosphate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
6.5 Enzymes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
Chapter 7: Cellular Respiration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 281
7.1 Energy in Living Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282
7.2 Glycolysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286
7.3 Oxidation of Pyruvate and the Citric Acid Cycle . . . . . . . . . . . . . . . . . . . . . . 291
7.4 Oxidative Phosphorylation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 296
7.5 Metabolism without Oxygen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 301
7.6 Connections of Carbohydrate, Protein, and Lipid Metabolic Pathways . . . . . . . . . . 307
7.7 Regulation of Cellular Respiration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 311
Chapter 8: Photosynthesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 329
8.1 Overview of Photosynthesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
8.2 The Light-Dependent Reaction of Photosynthesis . . . . . . . . . . . . . . . . . . . . 336
8.3 Using Light to Make Organic Molecules . . . . . . . . . . . . . . . . . . . . . . . . . . 347
Chapter 9: Cell Communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 365
9.1 Signaling Molecules and Cellular Receptors . . . . . . . . . . . . . . . . . . . . . . . 366
9.2 Propagation of the Signal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 376
9.3 Response to the Signal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 382
9.4 Signaling in Single-Celled Organisms . . . . . . . . . . . . . . . . . . . . . . . . . . . 386
Chapter 10: Cell Reproduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 403
10.1 Cell Division . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 403
10.2 The Cell Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 408
10.3 Control of the Cell Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 419
10.4 Cancer and the Cell Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 426
10.5 Prokaryotic Cell Division . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 430
Unit 3
Chapter 11: Meiosis and Sexual Reproduction . . . . . . . . . . . . . . . . . . . . . . . . . 445
11.1 The Process of Meiosis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 446
11.2 Sexual Reproduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 456
Chapter 12: Mendel's Experiments and Heredity . . . . . . . . . . . . . . . . . . . . . . . . 469
12.1 Mendel’s Experiments and the Laws of Probability . . . . . . . . . . . . . . . . . . . 470
12.2 Characteristics and Traits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 477
12.3 Laws of Inheritance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 494
Chapter 13: Modern Understandings of Inheritance . . . . . . . . . . . . . . . . . . . . . . 517
13.1 Chromosomal Theory and Genetic Linkages . . . . . . . . . . . . . . . . . . . . . . 518
13.2 Chromosomal Basis of Inherited Disorders . . . . . . . . . . . . . . . . . . . . . . . 524
Chapter 14: DNA Structure and Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . 543
14.1 Historical Basis of Modern Understanding . . . . . . . . . . . . . . . . . . . . . . . . 544
14.2 DNA Structure and Sequencing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 549
14.3 Basics of DNA Replication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 559
14.4 DNA Replication in Prokaryotes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 562
14.5 DNA Replication in Eukaryotes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 566
14.6 DNA Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 569
Chapter 15: Genes and Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 593
15.1 The Genetic Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 593
15.2 Prokaryotic Transcription . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 600
15.3 Eukaryotic Transcription . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 604
15.4 RNA Processing in Eukaryotes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 610
15.5 Ribosomes and Protein Synthesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . 615
Chapter 16: Gene Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 635
16.1 Regulation of Gene Expression . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 636
16.2 Prokaryotic Gene Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 639
16.3 Eukaryotic Epigenetic Gene Regulation . . . . . . . . . . . . . . . . . . . . . . . . . 645
16.4 Eukaryotic Transcriptional Gene Regulation . . . . . . . . . . . . . . . . . . . . . . . 649
16.5 Eukaryotic Post-transcriptional Gene Regulation . . . . . . . . . . . . . . . . . . . . 653
16.6 Eukaryotic Translational and Post-translational Gene Regulation . . . . . . . . . . . . 657
16.7 Cancer and Gene Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 659
Chapter 17: Biotechnology and Genomics . . . . . . . . . . . . . . . . . . . . . . . . . . . . 677
17.1 Biotechnology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 678
17.2 Mapping Genomes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 693
17.3 Whole-Genome Sequencing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 697
17.4 Applying Genomics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 701
17.5 Genomics and Proteomics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 704
Unit 4
Chapter 18: Evolution and Origin of Species . . . . . . . . . . . . . . . . . . . . . . . . . . 719
18.1 Understanding Evolution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 720
18.2 Formation of New Species . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 731
18.3 Reconnection and Rates of Speciation . . . . . . . . . . . . . . . . . . . . . . . . . 743
Chapter 19: The Evolution of Populations . . . . . . . . . . . . . . . . . . . . . . . . . . . . 767
19.1 Population Evolution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 768
19.2 Population Genetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 774
19.3 Adaptive Evolution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 783
Chapter 20: Phylogenies and the History of Life . . . . . . . . . . . . . . . . . . . . . . . . 807
20.1 Organizing Life on Earth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 808
20.2 Determining Evolutionary Relationships . . . . . . . . . . . . . . . . . . . . . . . . . 815
20.3 Perspectives on the Phylogenetic Tree . . . . . . . . . . . . . . . . . . . . . . . . . 826
Unit 5
Chapter 21: Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 849
21.1 Viral Evolution, Morphology, and Classification . . . . . . . . . . . . . . . . . . . . . 850
21.2 Virus Infection and Hosts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 857
21.3 Prevention and Treatment of Viral Infections . . . . . . . . . . . . . . . . . . . . . . 866
21.4 Other Acellular Entities: Prions and Viroids . . . . . . . . . . . . . . . . . . . . . . . 871
Chapter 22: Prokaryotes: Bacteria and Archaea . . . . . . . . . . . . . . . . . . . . . . . . . 889
22.1 Prokaryotic Diversity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 890
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22.2 Structure of Prokaryotes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 897
22.3 Prokaryotic Metabolism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 907
22.4 Bacterial Diseases in Humans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 911
22.5 Beneficial Prokaryotes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 919
Unit 6
Chapter 23: Plant Form and Physiology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 945
23.1 The Plant Body . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 946
23.2 Stems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 948
23.3 Roots . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 957
23.4 Leaves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 960
23.5 Transport of Water and Solutes in Plants . . . . . . . . . . . . . . . . . . . . . . . . 969
23.6 Plant Sensory Systems and Responses . . . . . . . . . . . . . . . . . . . . . . . . . 979
Unit 7
Chapter 24: The Animal Body: Basic Form and Function . . . . . . . . . . . . . . . . . . . 1017
24.1 Animal Form and Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1018
24.2 Animal Primary Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1026
24.3 Homeostasis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1037
Chapter 25: Animal Nutrition and the Digestive System . . . . . . . . . . . . . . . . . . . 1057
25.1 Digestive Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1058
25.2 Nutrition and Energy Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1072
25.3 Digestive System Processes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1080
25.4 Digestive System Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1087
Chapter 26: The Nervous System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1107
26.1 Neurons and Glial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1108
26.2 How Neurons Communicate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1116
26.3 The Central Nervous System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1130
26.4 The Peripheral Nervous System . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1137
26.5 Nervous System Disorders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1142
Chapter 27: Sensory Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1165
27.1 Sensory Processes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1166
27.2 Somatosensation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1170
27.3 Taste and Smell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1175
27.4 Hearing and Vestibular Sensation . . . . . . . . . . . . . . . . . . . . . . . . . . . 1180
27.5 Vision . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1187
Chapter 28: The Endocrine System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1209
28.1 Types of Hormones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1209
28.2 How Hormones Work . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1213
28.3 Regulation of Body Processes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1217
28.4 Regulation of Hormone Production . . . . . . . . . . . . . . . . . . . . . . . . . . 1230
28.5 Endocrine Glands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1232
Chapter 29: The Musculoskeletal System . . . . . . . . . . . . . . . . . . . . . . . . . . . 1257
29.1 Types of Skeletal Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1258
29.2 Bone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1270
29.3 Joints and Skeletal Movement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1278
29.4 Muscle Contraction and Locomotion . . . . . . . . . . . . . . . . . . . . . . . . . . 1287
Chapter 30: The Respiratory System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1309
30.1 Systems of Gas Exchange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1309
30.2 Gas Exchange across Respiratory Surfaces . . . . . . . . . . . . . . . . . . . . . 1318
30.3 Breathing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1324
30.4 Transport of Gases in Human Bodily Fluids . . . . . . . . . . . . . . . . . . . . . . 1332
Chapter 31: The Circulatory System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1349
31.1 Overview of the Circulatory System . . . . . . . . . . . . . . . . . . . . . . . . . . 1350
31.2 Components of the Blood . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1354
31.3 Mammalian Heart and Blood Vessels . . . . . . . . . . . . . . . . . . . . . . . . . 1362
31.4 Blood Flow and Blood Pressure Regulation . . . . . . . . . . . . . . . . . . . . . . 1372
Chapter 32: Osmotic Regulation and Excretion . . . . . . . . . . . . . . . . . . . . . . . . 1393
32.1 Osmoregulation and Osmotic Balance . . . . . . . . . . . . . . . . . . . . . . . . . 1394
32.2 The Kidneys and Osmoregulatory Organs . . . . . . . . . . . . . . . . . . . . . . . 1398
32.3 Excretion Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1407
32.4 Nitrogenous Wastes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1410
32.5 Hormonal Control of Osmoregulatory Functions . . . . . . . . . . . . . . . . . . . . 1414
Chapter 33: The Immune System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1437
33.1 Innate Immune Response . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1438
33.2 Adaptive Immune Response . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1446
33.3 Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1461
33.4 Disruptions in the Immune System . . . . . . . . . . . . . . . . . . . . . . . . . . 1467
Chapter 34: Animal Reproduction and Development . . . . . . . . . . . . . . . . . . . . . 1487
34.1 Reproduction Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1488
34.2 Fertilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1494
34.3 Human Reproductive Anatomy and Gametogenesis . . . . . . . . . . . . . . . . . 1496
34.4 Hormonal Control of Human Reproduction . . . . . . . . . . . . . . . . . . . . . . 1504
34.5 Fertilization and Early Embryonic Development . . . . . . . . . . . . . . . . . . . . 1510
34.6 Organogenesis and Vertebrate Formation . . . . . . . . . . . . . . . . . . . . . . . 1515
34.7 Human Pregnancy and Birth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1518
Unit 8
Chapter 35: Ecology and the Biosphere . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1539
35.1 The Scope of Ecology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1540
35.2 Biogeography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1545
35.3 Terrestrial Biomes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1552
35.4 Aquatic Biomes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1560
35.5 Climate and the Effects of Global Climate Change . . . . . . . . . . . . . . . . . . 1568
Chapter 36: Population and Community Ecology . . . . . . . . . . . . . . . . . . . . . . . 1593
36.1 Population Demography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1594
36.2 Life Histories and Natural Selection . . . . . . . . . . . . . . . . . . . . . . . . . . 1600
36.3 Environmental Limits to Population Growth . . . . . . . . . . . . . . . . . . . . . . 1605
36.4 Population Dynamics and Regulation . . . . . . . . . . . . . . . . . . . . . . . . . 1611
36.5 Human Population Growth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1616
36.6 Community Ecology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1622
36.7 Behavioral Biology: Proximate and Ultimate Causes of Behavior . . . . . . . . . . . 1636
Chapter 37: Ecosystems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1683
37.1 Ecology for Ecosystems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1683
37.2 Energy Flow through Ecosystems . . . . . . . . . . . . . . . . . . . . . . . . . . . 1695
37.3 Biogeochemical Cycles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1702
Chapter 38: Conservation Biology and Biodiversity . . . . . . . . . . . . . . . . . . . . . 1727
38.1 The Biodiversity Crisis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1728
38.2 The Importance of Biodiversity to Human Life . . . . . . . . . . . . . . . . . . . . . 1739
38.3 Threats to Biodiversity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1743
38.4 Preserving Biodiversity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1754
Appendix A: The Periodic Table of Elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1773
Appendix B: Geological Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1775
Appendix C: Measurements and the Metric System . . . . . . . . . . . . . . . . . . . . . . . . 1777
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1779

Preface 1
PREFACE
Welcome to Biology for AP® Courses, an OpenStax resource. This textbook was written to increase student access to high-
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About Biology for AP® Courses

Biology for AP® Courses covers the scope and sequence requirements of a typical two-semester Advanced Placement®
biology course. The text provides comprehensive coverage of core biology concepts and foundational research through an
evolutionary lens. Biology for AP® Courses was designed to meet and exceed the requirements of the College Board’s AP®
Biology framework while allowing significant flexibility for instructors. Each section of the book includes an introduction
based on the AP® curriculum as well as rich features that engage students in scientific practice and AP® test preparation. It
also highlights careers and research opportunities in the biological sciences.
Coverage and scope

In developing Biology for AP® Courses, we relied on experts in the goals and approach of the AP® curriculum, carefully
considered the AP® framework design, and listened to the advice of hundreds of high school and college biology instructors.
The result is a book that provides excellent coverage of the AP® framework while addressing the sheer breadth of biology
topics in the modern age. We provide a thorough treatment of biology’s foundational concepts while condensing selected
topics. We also strive to make biology, as a discipline, interesting and accessible to students. In addition to a comprehensive
2 Preface
coverage of core concepts and foundational research, we have incorporated features that draw learners into the discipline in
meaningful ways.
Unit 1: The Chemistry of Life. Our opening unit introduces students to the sciences, including scientific methods
and the fundamental concepts of chemistry and physics that provide a framework within which learners comprehend
biological processes.
Unit 2: The Cell. Students will gain solid understanding of the structures, functions, and processes of the most basic
unit of life: the cell.
Unit 3: Genetics. Our comprehensive genetics unit takes learners from the earliest experiments that revealed the basis
of inheritance through the intricacies of DNA’s structure, replication, and expression, to current applications in the
studies of biotechnology and genomics.
Unit 4: Evolutionary Processes. The core concepts of evolution are discussed in this unit with examples illustrating
evolutionary processes. Additionally, the evolutionary basis of biology reappears throughout the textbook in general
discussion and is also reinforced through special call-out features highlighting specific topics in evolution.
Unit 5: Biological Diversity. The diversity of life is explored through detailed study of all phyla of organisms as well
as discussion of their phylogenetic relationships. The unit begins with viruses and then moves through prokaryotes and
eukaryotes, ending with a discussion of vertebrates and, finally, humans.
Unit 6: Plant Structure and Function. Our plant anatomy and physiology unit thoroughly covers the fundamental
structure and function of plant cells, tissues, and organs. It also covers important plant physiological functions such as
tissue differentiation, material transport, and the roles of plant hormones.
Unit 7: Animal Structure and Function. An introduction to the form and function of the animal body is followed
by detailed chapters on specific body systems and their physiological function. This unit touches on the biology of all
organisms while maintaining an engaging focus on human anatomy and physiology that helps students connect to the
topics.
Unit 8: Ecology. Ecological concepts are broadly covered in this unit, beginning with the small-scale relationships
of population ecology and gradually building to the large-scale processes of ecosystem ecology. Localized, real-world
issues of conservation and biodiversity are presented at numerous points along the way.
AP® Connections
Every section of the textbook — over 200 total — begins with a “Connection for AP® Courses.” Section. Written by
Julianne Zedalis, the College Board’s AP® Biology Curriculum Committee Co-Chair, these valuable overviews provide
meaningful support for students and instructors.
Each Connection highlights the key concepts of the section in the context of the AP® Biology Curriculum Framework
and explains their importance in brief, engaging language.
The explanations build upon the knowledge gained in previous sections, reinforcing the most significant concepts
and alerting students of the foundational basis of upcoming material. This helps students build a more comprehensive
understanding and helps instructors reference prior explanations.
Direct references to the relevant sections of the AP® Curriculum Framework are first explained and then outlined in
table format emphasizing their importance and relating them to the overall design of the course. Students and teachers
using these reference tables can easily see their progression through and coverage of the required curriculum.
Scientific practices
The AP® Biology Science Practices are presented to students through several active learning features.
Science Practice Connections for AP® Courses provide a context and suggested activity linking the concepts with the
relevant science practices. Students are often asked to build representations, undertake brief research, or answer critical
thinking questions.
Science Practice Questions, designed and authored by John Eggebrecht and Julianne Zedalis, present a complex scenario
or data set and ask students a series of multiple-choice and open-ended questions based on a complex scenario or data
set. These robust activities hone students’ scientific thinking skills and prepare them for similar questions on the AP®
Examination.
Pedagogical foundation and features

Biology for AP® Courses® is grounded in a solid scientific base, with features that engage the students in scientific inquiry:

Preface 3
Evolution Connection features highlight the importance and relevance of evolutionary theory to all biological
study. Through discussions like “The Evolution of Metabolic Pathways” and “Algae and Evolutionary Paths to
Photosynthesis,” the student is able to see how evolution pervades all aspects of biology.
Scientific Methods Connection call-outs walk students through actual or thought experiments that elucidate scientific
processes and procedures for a variety of topics. Features include “Determining the Time Spent in Cell Cycle Stages”
and “Testing the Hypothesis of Independent Assortment.”
Career Connection features present information on a variety of careers in the biological sciences. They are meant
to introduce students to professions and day-to-day work related to the current section content. Examples include
microbiologist, ecologist, neurologist, and forensic scientist.
Everyday Connection features tie biological concepts to students’ everyday lives as well as emerging world issues
related to biology. Topics include “Chesapeake Bay” and “Can Snail Venom Be Used as a Pharmacological Pain
Killer?”
Illustrations and animations that engage

Illustrations within the book are designed to help students visualize the concepts of biology using figures with simple,
clear, designs and color schemes that go side-by-side with vivid photos and micrographs. Biology for AP® Courses also
incorporates links to relevant animations and interactive exercises that help bring biology to life.
Visual Connection features identify core figures in each chapter for student study. Questions about key figures,
including clicker questions that can be used in the classroom, engage students’ critical thinking to ensure genuine
understanding.
Link to Learning features direct students to online interactive exercises and animations that add greater context to
core content.
Additional resources
Student and instructor resources
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guide. Instructor resources require a verified instructor account, which you can apply for when you log in or create your
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About the authors

Senior contributing authors
Julianne Zedalis, Bishop’s School, La Jolla, California
Julianne Zedalis has taught AP® Biology for over twenty years. She served on the College Board’s committee to rewrite
and test the revised AP® Curriculum Framework, working with other high school AP® teachers and college faculty as
well as the National Science Foundation. She was later selected to chair the College Board’s Curriculum Development and
4 Preface
Assessment Committee.
John Eggebrecht
Dr. John Eggebrecht, Brooklyn Technical High School (retired), Brooklyn, New York
John Eggebrecht taught AP® Physics and Biology courses for over thirty years. He was instrumental in the development
and revision of various AP® curriculum frameworks over an extended collaboration with the College Board and other
educational organizations. Under his guidance, Brooklyn Tech was repeatedly selected by the College Board as an
exemplary AP® program, and its practices and students outcomes were featured in several publications. In addition to his
writing role, John regularly evaluates course materials and programs for alignment and quality.
Contributing authors
Connie Rye, East Mississippi Community College
Robert Wise, The University of Wisconsin Oshkosh
Vladimir Jurukovski, Suffolk County Community College
Jean DeSaix, The University of North Carolina at Chapel Hill
Jung Choi, Georgia Institute of Technology
Yael Avissar, Rhode Island College
Curriculum Framework for AP® Biology

Big Idea 1: The process of evolution drives the diversity and unity of life.
Enduring understanding 1.A. Change in the genetic makeup of a
Chapter/Key Concepts
population over time is evolution.
5.3, 18.1, 18.2, 19.1, 19.2,
1.A.1. Natural selection is a major mechanism of evolution.
19.3, 21.2, 23.5
1.A.2. Natural selection acts on phenotypic variations in populations. 7.3, 7.6, 18.2, 19.2, 19.3, 36.5
1.A.3. Evolutionary change is also driven by random processes. 19.1, 19.2
1.A.4. Biological evolution is supported by scientific evidence from many 2.1, 5.2, 8.2, 11.1, 14.1, 17.1
disciplines, including mathematics. 18.1, 19.3
Enduring understanding 1.B. Organisms are linked by lines of descent
from common ancestry.
1.B.1. Organisms share many conserved core processes and features that 3.4, 4.3, 4.6, 8.2, 15.3, 13.2,
evolved and are widely distributed among organisms today. 14.1, 15.5, 18.1, 20.1, 20.2
1.B.2. Phylogenetic trees and cladograms are graphical representations
14.4, 20.1, 20.2, 20.3
(models) of evolutionary history that can be tested.
Enduring understanding 1.C. Life continues to evolve within a changing
environment.
1.C.1. Speciation and extinction have occurred throughout the Earth's
14.4, 18.2, 20.1, 38.1
history.
1.C.2. Speciation may occur when two populations become reproductively
18.2, 19.2, 23.5
isolated from each other.
7.3, 7.6, 18.1, 18.3, 19.1, 19.2,
1.C.3. Populations of organisms continue to evolve.
20.1, 20.2, 23.5
Enduring understanding 1.D. The origin of living systems is explained by
natural processes.
1.D.1. There are several hypotheses about the natural origin of life on Earth,
8.2, 18.1, 20.1, 21.1, 20.3
each with supporting scientific evidence.
1.D.2. Scientific evidence from many different disciplines supports models of
8.2, 18.1, 20.2, 28.1
the origin of life.

Preface 5
Big Idea 2: Biological systems utilize free energy and molecular building blocks
to grow, to reproduce and to maintain dynamic homeostasis.
Enduring understanding 2.A. Growth, reproduction and maintenance of
the organization of living systems require free energy and matter.
6.1, 6.2, 6.3, 6.4, 6.7, 7.1, 7.2, 7.3,
2.A.1. All living systems require constant input of free energy 7.4, 7.5, 7.6, 8.2, 23.1, 23.5, 36.3,
37.2
3.2, 4.3, 6.1, 6.4, 6.5, 7.1, 7.2, 7.3,
2.A.2. Organisms capture and store free energy for use in biological
7.4, 7.4, 7.5, 7.6, 8.1, 8.2, 8.3, 9.2,
processes.
22.1, 22.2, 23.1, 23.5, 37.2
2.A.3. Organisms must exchange matter with the environment to grow, 2.1, 2.2, 3.3, 4.2, 4.6, 6.1, 6.8,
reproduce and maintain organization. 22.4, 22.5, 23.5, 25.8, 37.3
Enduring understanding 2.B. Growth, reproduction and dynamic
homeostasis require that cells create and maintain internal environments Chapter/Key Concepts
that are different from their external environments.
2.B.1. Cell membranes are selectively permeable due to their structure. 3.2, 3.3, 5.1, 5.2, 5.3, 5.4, 8.3
2.B.2. Growth and dynamic homeostasis are maintained by the constant
2.3, 3.3, 5.2, 5.3, 5.4
movement of molecules across membranes.
2.B.3. Eukaryotic cells maintain internal membranes that partition the cell
3.3, 4.2, 4.3, 4.4
into specialized regions.
Enduring understanding 2.C. Organisms use feedback mechanisms to
regulate growth and reproduction, and to maintain dynamic homeostasis.
5.2, 5.3, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6,
2.C.1. Organisms use feedback mechanisms to maintain their internal
8.2, 10.1, 11.1, 21.1, 23.5, 24.3,
environments and respond to external environmental changes.
28.3
2.1, 6.4, 7.5, 7.6, 22.5, 23.5, 26.3,
2.C.2. Organisms respond to changes in their external environments.
26.5
Enduring understanding 2.D. Growth and dynamic homeostasis of a
biological system are influenced by changes in the system's environment.
2.D.1. All biological systems from cells and organisms to populations, 2.1, 2.2, 7.1, 7.4, 7.5, 7.6, 8.2,
communities and ecosystems are affected by complex biotic and abiotic 15.2, 15.3, 17.3, 21.1, 22.4, 35.1,
interactions involving exchange of matter and free energy. 37.1
2.D.2. Homeostatic mechanisms reflect both common ancestry and 4.3, 5.2, 6.1, 18.2, 21.1, 25.1, 32.1,
divergence due to adaptation in different environments. 32.3, 34.1
2.D.3. Biological systems are affected by disruptions to their dynamic
3.2, 22.3, 22.5, 23.1, 28.3, 38.2
homeostasis.
2.D.4. Plants and animals have a variety of chemical defenses against
23.6, 33.1, 33.2
infections that affect dynamic homeostasis.
Enduring understanding 2.E. Many biological processes involved in
growth, reproduction and dynamic homeostasis include temporal Chapter/Key Concepts
regulation and coordination.
2.E.1. Timing and coordination of specific events are necessary for the
10.2, 10.3, 14.3, 23.5, 30.1, 32.3,
normal development of an organism, and these events are regulated by a
34.1, 34.6
variety of mechanisms.
6.8, 10.1, 10.2, 15.3, 22.3, 23.2,
2.E.2. Timing and coordination of physiological events are regulated by
24.1, 30.6, 36.1, 36.2, 36.3, 36.4,
multiple mechanisms.
36.5, 43.6, 43.7
6 Preface
Big Idea 2: Biological systems utilize free energy and molecular building blocks
to grow, to reproduce and to maintain dynamic homeostasis.
2.E.3. Timing and coordination of behavior are regulated by various
11.1, 21.2, 23.5, 30.6, 35.2, 45.7
mechanisms and are important in natural selection.
Big Idea 3: Living systems store, retrieve, transmit and respond to information
essential to life processes.
Enduring understanding 3.A. Heritable information provides
for continuity of life.
3.5, 10.3, 13.1, 13.2, 14.1, 14.2, 14.3, 14.5,
3.A.1. DNA, and in some cases RNA, is the primary source of
15.1, 15.2, 15.3, 15.4, 15.5, 16.1, 16.2, 16.3,
heritable information.
17.1, 17.3, 21.1, 21.2, 22.4
3.A.2. In eukaryotes, heritable information is passed to the
next generation via processes that include the cell cycle and 10.1, 10.2, 10.3, 11.1, 11.2, 13.1
mitosis or meiosis plus fertilization.
3.A.3. The chromosomal basis of inheritance provides an
understanding of the pattern of passage (transmission) of 11.2, 12.1, 12.2, 13.1, 14.2, 17.1, 17.4
genes from parent to offspring.
3.A.4. The inheritance pattern of many traits cannot be
4.3, 12.2, 13.1
explained by simple Mendelian genetics.
Enduring understanding 3.B. Expression of genetic
information involves cellular and molecular mechanisms.
3.B.1. Gene regulation results in differential gene expression,
7.3, 7.6, 16.1, 16.2, 16.3, 16.4, 16.5, 17.3
leading to cell specialization.
3.B.2. A variety of intercellular and intracellular signal
9.1, 9.2, 9.3, 15.3, 17.1
transmissions mediate gene expression.
Enduring understanding 3.C. The processing of genetic
information is imperfect and is a source of genetic variation.
3.C.1. Changes in genotype can result in changes in 5.3, 11.2, 13.1, 13.2, 14.6, 15.1, 17.1, 18.1,
phenotype. 19.1, 19.3
3.C.2. Biological systems have multiple processes that 11.2, 13.1, 14.1, 14.6, 15.2, 17.1, 20.3, 21.2,
increase genetic variation. 22.4
3.C.3. Viral replication results in genetic variation, and viral
21.1, 21.2
infection can introduce genetic variation into the hosts.
Enduring understanding 3.D. Cells communicate by
generating, transmitting and receiving chemical signals
3.D.1. Cell communication processes share common features
4.6, 9.1, 9.2, 9.3, 9.4, 10.4, 37.2, 37.3
that reflect a shared evolutionary history.
3.D.2. Cells communicate with each other through direct
contact with other cells or from a distance via chemical 9.1, 9.3
signaling.
3.D.3. Signal transduction pathways link signal reception with
9.1, 9.2
cellular response.
3.D.4. Changes in signal transduction pathways can alter
9.2, 9.3, 9.4
cellular response.

Preface 7
Big Idea 3: Living systems store, retrieve, transmit and respond to information
essential to life processes.
Enduring understanding 3.E. Transmission of information
results in changes within and between biological systems.
3.E.1. Individuals can act on information and communicate it
9.2, 9.4, 21.2, 36.1, 36.2, 36.3, 36.4, 36.5
to others.
3.E.2. Animals have nervous systems that detect external and
internal signals, transmit and integrate information, and 6.1, 35.1, 35.2, 35.3, 35.4
produce responses.
Big Idea 4: Biological systems interact, and these systems and their interactions
possess complex properties.
Enduring understanding 4.A Interactions within biological systems lead
to complex properties.
4.A.1. The subcomponents of biological molecules and their sequence 3.1, 3.2, 3.3, 3.4, 3.5, 5.2, 6.2, 14.1,
determine the properties of that molecule. 14.3, 14.4, 17.1
4.A.2. The structure and function of subcellular components, and their
3.4, 4.3, 4.4, 4.6, 10.3, 15.3
interactions, provide essential cellular processes.
4.A.3. Interactions between external stimuli and regulated gene
16.1, 22.3, 43.6, 43.7
expression result in specialization of cells, tissues and organs.
4.A.4. Organisms exhibit complex properties due to interactions between 15.2, 17.1, 18.1, 22.3, 22.5, 30.5,
their constituent parts. 33.3, 34.3
4.A.5. Communities are composed of populations of organisms that
22.5, 23.5, 45.5, 45.6
interact in complex ways.
3.2, 3.3, 6.2, 6.3, 6.6, 7.5, 7.6, 8.2,
4.A.6. Interactions among living systems and with their environment
10.3, 18.1, 23.1, 22.4, 45.2, 45.6,
result in the movement of matter and energy.
46.2, 47.3
Enduring understanding 4.B Competition and cooperation are
important aspects of biological systems.
4.B.1. Interactions between molecules affect their structure and function. 3.5, 5.2, 6.2, 6.5, 8.3
4.B.2. Cooperative interactions within organisms promote efficiency in
4.3, 7.3, 7.6, 45.6
the use of energy and matter.
4.B.3. Interactions between and within populations influence patterns of
45.4, 45.6
species distribution and abundance.
4.B.4. Distribution of local and global ecosystems changes over time. 22.4, 23.1, 46.1, 47.1, 47.3
Enduring understanding 4.C. Naturally occurring diversity among and
between components within biological systems affects interactions with Chapter/Key Concepts
the environment.
4.C.1. Variation in molecular units provides cells with a wider range of
3.4, 9.2, 10.3, 13.1, 15.5, 42.2, 49.1
functions.
4.C.2. Environmental factors influence the expression of the genotype in
14.2, 19.3, 22.3, 30.4, 43.1
an organism.
4.C.3. The level of variation in a population affects population dynamics. 7.5, 7.6, 19.1, 45.6, 47.1
8 Preface
Big Idea 4: Biological systems interact, and these systems and their interactions
possess complex properties.
4.C.4. The diversity of species within an ecosystem may influence the
45.6, 46.1
stability of the ecosystem.

Chapter 1 | The Study of Life 9
1 | THE STUDY OF LIFE
Figure 1.1 This NASA image is a composite of several satellite-based views of Earth. To make the whole-Earth image,
NASA scientists combine observations of different parts of the planet. (credit: NASA/GSFC/NOAA/USGS)
Chapter Outline
1.1: The Science of Biology
1.2: Themes and Concepts of Biology
Introduction
Viewed from space, Earth offers no clues about the diversity of life it harbors. The first forms of life on Earth are thought
be microorganisms that existed for billions of years in the ocean before plants and animals appeared. The mammals, birds,
and flowers that we see in modern times are mostly “recent” species, originating 130 to 200 million years ago. In fact, only
in the last 200,000 years have humans started looking like we do today.
Organisms evolve in response to each other. One of the best examples is disease causing organisms, which have to adapt
to overcome the defenses of the organisms they infect. One such organism that has evolved to specialize in infection in
humans is Plasmodium, the organism that causes malaria. Biologists use the process of science to learn about the world and
the organisms living in it. For example, people have suspected for quite some time that people with blood type O are less
likely to die from severe malaria. Now, a team of scientists have been able to explain why. By examining data from several
experiments, and by using both inductive and deductive reasoning, the scientists concluded that A and B type blood reacts
with a protein excreted by Plasmodium. This reaction causes severe illness. However, type O blood does not react with
the protein. You can read more (http://openstaxcollege.org/l/32plasmodium) about the response of type A and B blood
groups to infection by Plasmodium.
1.1 | The Science of Biology

In this section, you will explore the following questions:
• What are the characteristics shared by the natural sciences?
• What are the steps of the scientific method?
10 Chapter 1 | The Study of Life
Connection for AP® courses

Biology is the science that studies living organisms and their interactions with one another and with their environment. The
process of science attempts to describe and understand the nature of the universe by rational means. Science has many fields;
those fields related to the physical world, including biology, are considered natural sciences. All of the natural sciences
follow the laws of chemistry and physics. For example, when studying biology, you must remember living organisms obey
the laws of thermodynamics while using free energy and matter from the environment to carry out life processes that are
explored in later chapters, such as metabolism and reproduction.
Two types of logical reasoning are used in science: inductive reasoning and deductive reasoning. Inductive reasoning
uses particular results to produce general scientific principles. Deductive reasoning uses logical thinking to predict results
by applying scientific principles or practices. The scientific method is a step-by-step process that consists of: making
observations, defining a problem, posing hypotheses, testing these hypotheses by designing and conducting investigations,
and drawing conclusions from data and results. Scientists then communicate their results to the scientific community.
Scientific theories are subject to revision as new information is collected.
The content presented in this section supports the Learning Objectives outlined in Big Idea 2 of the AP® Biology
Curriculum Framework. The Learning Objectives merge Essential Knowledge content with one or more of the seven
Science Practices. These objectives provide a transparent foundation for the AP® Biology course, along with inquiry-based
laboratory experiences, instructional activities, and AP® Exam questions.
Biological systems utilize free energy and molecular building blocks to grow, to
Big Idea 2
reproduce, and to maintain dynamic homeostasis.
Enduring
Growth, reproduction and maintenance of living systems require free energy and matter.
Understanding 2.A
Essential
2.A.1 All living systems require constant input of free energy.
Knowledge
6.4 The student can make claims and predictions about natural phenomena based on
Science Practice
scientific theories and models
Learning 2.3 The student is able to predict how changes in free energy availability affect
Objectives organisms, populations and ecosystems.
(a) (b)
Figure 1.2 Formerly called blue-green algae, these (a) cyanobacteria, shown here at 300x magnification under a light
microscope, are some of Earth’s oldest life forms. These (b) stromatolites along the shores of Lake Thetis in Western
Australia are ancient structures formed by the layering of cyanobacteria in shallow waters. (credit a: modification of
work by NASA; credit b: modification of work by Ruth Ellison; scale-bar data from Matt Russell)
What is biology? In simple terms, biology is the study of living organisms and their interactions with one another and their
environments. This is a very broad definition because the scope of biology is vast. Biologists may study anything from
the microscopic or submicroscopic view of a cell to ecosystems and the whole living planet (Figure 1.2). Listening to the
daily news, you will quickly realize how many aspects of biology are discussed every day. For example, recent news topics

include Escherichia coli (Figure 1.3) outbreaks in spinach and Salmonella contamination in peanut butter. On a global
scale, many researchers are committed to finding ways to protect the planet, solve environmental issues, and reduce the
effects of climate change. All of these diverse endeavors are related to different facets of the discipline of biology.
Figure 1.3 Escherichia coli (E. coli) bacteria, seen in this scanning electron micrograph, are normal residents of our
digestive tracts that aid in the absorption of vitamin K and other nutrients. However, virulent strains are sometimes
responsible for disease outbreaks. (credit: Eric Erbe, digital colorization by Christopher Pooley, both of USDA, ARS,
EMU)
The Process of Science

Biology is a science, but what exactly is science? What does the study of biology share with other scientific disciplines?
Science (from the Latin scientia, meaning “knowledge”) can be defined as knowledge that covers general truths or the
operation of general laws, especially when acquired and tested by the scientific method. It becomes clear from this definition
that the application of the scientific method plays a major role in science. The scientific method is a method of research
with defined steps that include experiments and careful observation.
The steps of the scientific method will be examined in detail later, but one of the most important aspects of this method is
the testing of hypotheses by means of repeatable experiments. A hypothesis is a suggested explanation for an event, which
can be tested. Although using the scientific method is inherent to science, it is inadequate in determining what science is.
This is because it is relatively easy to apply the scientific method to disciplines such as physics and chemistry, but when it
comes to disciplines like archaeology, psychology, and geology, the scientific method becomes less applicable as it becomes
more difficult to repeat experiments.
These areas of study are still sciences, however. Consider archaeology—even though one cannot perform repeatable
experiments, hypotheses may still be supported. For instance, an archaeologist can hypothesize that an ancient culture
existed based on finding a piece of pottery. Further hypotheses could be made about various characteristics of this culture,
and these hypotheses may be found to be correct or false through continued support or contradictions from other findings. A
hypothesis may become a verified theory. A theory is a tested and confirmed explanation for observations or phenomena.
Science may be better defined as fields of study that attempt to comprehend the nature of the universe.
Natural Sciences
What would you expect to see in a museum of natural sciences? Frogs? Plants? Dinosaur skeletons? Exhibits about how the
brain functions? A planetarium? Gems and minerals? Or, maybe all of the above? Science includes such diverse fields as
astronomy, biology, computer sciences, geology, logic, physics, chemistry, and mathematics (Figure 1.4). However, those
fields of science related to the physical world and its phenomena and processes are considered natural sciences. Thus, a
museum of natural sciences might contain any of the items listed above.
Figure 1.4 The diversity of scientific fields includes astronomy, biology, computer science, geology, logic, physics,
chemistry, mathematics, and many other fields. (credit: “Image Editor”/Flickr)
There is no complete agreement when it comes to defining what the natural sciences include, however. For some experts,
the natural sciences are astronomy, biology, chemistry, earth science, and physics. Other scholars choose to divide natural
sciences into life sciences, which study living things and include biology, and physical sciences, which study nonliving
matter and include astronomy, geology, physics, and chemistry. Some disciplines such as biophysics and biochemistry build
on both life and physical sciences and are interdisciplinary. Natural sciences are sometimes referred to as “hard science”
because they rely on the use of quantitative data; social sciences that study society and human behavior are more likely to
use qualitative assessments to drive investigations and findings.
Not surprisingly, the natural science of biology has many branches or subdisciplines. Cell biologists study cell structure
and function, while biologists who study anatomy investigate the structure of an entire organism. Those biologists studying
physiology, however, focus on the internal functioning of an organism. Some areas of biology focus on only particular types
of living things. For example, botanists explore plants, while zoologists specialize in animals.
Scientific Reasoning
One thing is common to all forms of science: an ultimate goal “to know.” Curiosity and inquiry are the driving forces for the
development of science. Scientists seek to understand the world and the way it operates. To do this, they use two methods
of logical thinking: inductive reasoning and deductive reasoning.
Inductive reasoning is a form of logical thinking that uses related observations to arrive at a general conclusion. This type
of reasoning is common in descriptive science. A life scientist such as a biologist makes observations and records them.
These data can be qualitative or quantitative, and the raw data can be supplemented with drawings, pictures, photos, or
videos. From many observations, the scientist can infer conclusions (inductions) based on evidence. Inductive reasoning
involves formulating generalizations inferred from careful observation and the analysis of a large amount of data. Brain
studies provide an example. In this type of research, many live brains are observed while people are doing a specific activity,
such as viewing images of food. The part of the brain that “lights up” during this activity is then predicted to be the part
controlling the response to the selected stimulus, in this case, images of food. The “lighting up” of the various areas of the
brain is caused by excess absorption of radioactive sugar derivatives by active areas of the brain. The resultant increase in
radioactivity is observed by a scanner. Then, researchers can stimulate that part of the brain to see if similar responses result.
Deductive reasoning or deduction is the type of logic used in hypothesis-based science. In deductive reason, the pattern
of thinking moves in the opposite direction as compared to inductive reasoning. Deductive reasoning is a form of logical

thinking that uses a general principle or law to forecast specific results. From those general principles, a scientist can
extrapolate and predict the specific results that would be valid as long as the general principles are valid. Studies in climate
change can illustrate this type of reasoning. For example, scientists may predict that if the climate becomes warmer in a
particular region, then the distribution of plants and animals should change. These predictions have been made and tested,
and many such changes have been found, such as the modification of arable areas for agriculture, with change based on
temperature averages.
Both types of logical thinking are related to the two main pathways of scientific study: descriptive science and hypothesis-
based science. Descriptive (or discovery) science, which is usually inductive, aims to observe, explore, and discover, while
hypothesis-based science, which is usually deductive, begins with a specific question or problem and a potential answer or
solution that can be tested. The boundary between these two forms of study is often blurred, and most scientific endeavors
combine both approaches. The fuzzy boundary becomes apparent when thinking about how easily observation can lead to
specific questions. For example, a gentleman in the 1940s observed that the burr seeds that stuck to his clothes and his dog’s
fur had a tiny hook structure. On closer inspection, he discovered that the burrs’ gripping device was more reliable than a
zipper. He eventually developed a company and produced the hook-and-loop fastener often used on lace-less sneakers and
athletic braces. Descriptive science and hypothesis-based science are in continuous dialogue.
The Scientific Method

Biologists study the living world by posing questions about it and seeking science-based responses. This approach is
common to other sciences as well and is often referred to as the scientific method. The scientific method was used even in
ancient times, but it was first documented by England’s Sir Francis Bacon (1561–1626) (Figure 1.5), who set up inductive
methods for scientific inquiry. The scientific method is not exclusively used by biologists but can be applied to almost all
fields of study as a logical, rational problem-solving method.
Figure 1.5 Sir Francis Bacon (1561–1626) is credited with being the first to define the scientific method. (credit: Paul
van Somer)
The scientific process typically starts with an observation (often a problem to be solved) that leads to a question. Let’s think
about a simple problem that starts with an observation and apply the scientific method to solve the problem. One Monday
morning, a student arrives at class and quickly discovers that the classroom is too warm. That is an observation that also
describes a problem: the classroom is too warm. The student then asks a question: “Why is the classroom so warm?”
Proposing a Hypothesis
Recall that a hypothesis is a suggested explanation that can be tested. To solve a problem, several hypotheses may be
proposed. For example, one hypothesis might be, “The classroom is warm because no one turned on the air conditioning.”
But there could be other responses to the question, and therefore other hypotheses may be proposed. A second hypothesis
might be, “The classroom is warm because there is a power failure, and so the air conditioning doesn’t work.”
Once a hypothesis has been selected, the student can make a prediction. A prediction is similar to a hypothesis but it
typically has the format “If . . . then . . . .” For example, the prediction for the first hypothesis might be, “If the student turns
on the air conditioning, then the classroom will no longer be too warm.”
Testing a Hypothesis
A valid hypothesis must be testable. It should also be falsifiable, meaning that it can be disproven by experimental
results. Importantly, science does not claim to “prove” anything because scientific understandings are always subject to
modification with further information. This step—openness to disproving ideas—is what distinguishes sciences from non-
sciences. The presence of the supernatural, for instance, is neither testable nor falsifiable. To test a hypothesis, a researcher
will conduct one or more experiments designed to eliminate one or more of the hypotheses. Each experiment will have one
or more variables and one or more controls. A variable is any part of the experiment that can vary or change during the
experiment. The control group contains every feature of the experimental group except it is not given the manipulation
that is hypothesized about. Therefore, if the results of the experimental group differ from the control group, the difference
must be due to the hypothesized manipulation, rather than some outside factor. Look for the variables and controls in
the examples that follow. To test the first hypothesis, the student would find out if the air conditioning is on. If the air
conditioning is turned on but does not work, there should be another reason, and this hypothesis should be rejected. To test
the second hypothesis, the student could check if the lights in the classroom are functional. If so, there is no power failure
and this hypothesis should be rejected. Each hypothesis should be tested by carrying out appropriate experiments. Be aware
that rejecting one hypothesis does not determine whether or not the other hypotheses can be accepted; it simply eliminates
one hypothesis that is not valid (see this figure). Using the scientific method, the hypotheses that are inconsistent with
experimental data are rejected.
While this “warm classroom” example is based on observational results, other hypotheses and experiments might have
clearer controls. For instance, a student might attend class on Monday and realize she had difficulty concentrating on the
lecture. One observation to explain this occurrence might be, “When I eat breakfast before class, I am better able to pay
attention.” The student could then design an experiment with a control to test this hypothesis.
In hypothesis-based science, specific results are predicted from a general premise. This type of reasoning is called deductive
reasoning: deduction proceeds from the general to the particular. But the reverse of the process is also possible: sometimes,
scientists reach a general conclusion from a number of specific observations. This type of reasoning is called inductive
reasoning, and it proceeds from the particular to the general. Inductive and deductive reasoning are often used in tandem to
advance scientific knowledge (see this figure)
Think About It
Almost all plants use water, carbon dioxide, and energy from the sun to make sugars. Think about what would happen
to plants that don’t have sunlight as an energy source or sufficient water. What would happen to organisms that depend
on those plants for their own survival?
Make a prediction about what would happen to the organisms living in a rain forest if 50% of its trees were destroyed.
How would you test your prediction?

Figure 1.6 The scientific method consists of a series of well-defined steps. If a hypothesis is not supported by
experimental data, a new hypothesis can be proposed.
In the example below, the scientific method is used to solve an everyday problem. Order the scientific method steps
(numbered items) with the process of solving the everyday problem (lettered items). Based on the results of the
experiment, is the hypothesis correct? If it is incorrect, propose some alternative hypotheses.
Scientific
Everyday process
Method
1 Observation A There is something wrong with the electrical outlet.
If something is wrong with the outlet, my coffeemaker also won’t work when
2 Question B
plugged into it.
3 Hypothesis (answer) C My toaster doesn’t toast my bread.
4 Prediction D I plug my coffee maker into the outlet.
5 Experiment E My coffeemaker works.
Scientific
Everyday process
Method
6 Result F What is preventing my toaster from working?
a. The original hypothesis is correct. There is something wrong with the electrical outlet and therefore the
toaster doesn’t work.
b. The original hypothesis is incorrect. Alternative hypothesis includes that toaster wasn’t turned on.
c. The original hypothesis is correct. The coffee maker and the toaster do not work when plugged into the
outlet.
d. The original hypothesis is incorrect. Alternative hypotheses includes that both coffee maker and toaster were
broken.

Figure 1.7 Scientists use two types of reasoning, inductive and deductive reasoning, to advance scientific
knowledge. As is the case in this example, the conclusion from inductive reasoning can often become the premise
for deductive reasoning.
Decide if each of the following is an example of inductive or deductive reasoning.

1. All flying birds and insects have wings. Birds and insects flap their wings as they move through the air. Therefore,
wings enable flight.
2. Insects generally survive mild winters better than harsh ones. Therefore, insect pests will become more
problematic if global temperatures increase.
3. Chromosomes, the carriers of DNA, separate into daughter cells during cell division. Therefore, DNA is the
genetic material.
4. Animals as diverse as insects and wolves all exhibit social behavior. Therefore, social behavior must have an
evolutionary advantage for humans.
a. 1- Inductive, 2- Deductive, 3- Deductive, 4- Inductive
b. 1- Deductive, 2- Inductive, 3- Deductive, 4- Inductive
c. 1- Inductive, 2- Deductive, 3- Inductive, 4- Deductive
d. 1- Inductive, 2-Inductive, 3- Inductive, 4- Deductive
The scientific method may seem too rigid and structured. It is important to keep in mind that, although scientists often
follow this sequence, there is flexibility. Sometimes an experiment leads to conclusions that favor a change in approach;
often, an experiment brings entirely new scientific questions to the puzzle. Many times, science does not operate in a linear
fashion; instead, scientists continually draw inferences and make generalizations, finding patterns as their research proceeds.
Scientific reasoning is more complex than the scientific method alone suggests. Notice, too, that the scientific method can
be applied to solving problems that aren’t necessarily scientific in nature.
Two Types of Science: Basic Science and Applied Science

The scientific community has been debating for the last few decades about the value of different types of science. Is it
valuable to pursue science for the sake of simply gaining knowledge, or does scientific knowledge only have worth if we
can apply it to solving a specific problem or to bettering our lives? This question focuses on the differences between two
types of science: basic science and applied science.
Basic science or “pure” science seeks to expand knowledge regardless of the short-term application of that knowledge.
It is not focused on developing a product or a service of immediate public or commercial value. The immediate goal of
basic science is knowledge for knowledge’s sake, though this does not mean that, in the end, it may not result in a practical
application.
In contrast, applied science or “technology,” aims to use science to solve real-world problems, making it possible, for
example, to improve a crop yield, find a cure for a particular disease, or save animals threatened by a natural disaster (Figure
1.8). In applied science, the problem is usually defined for the researcher.
Figure 1.8 After Hurricane Ike struck the Gulf Coast in 2008, the U.S. Fish and Wildlife Service rescued this brown
pelican. Thanks to applied science, scientists knew how to rehabilitate the bird. (credit: FEMA)
Some individuals may perceive applied science as “useful” and basic science as “useless.” A question these people might
pose to a scientist advocating knowledge acquisition would be, “What for?” A careful look at the history of science,
however, reveals that basic knowledge has resulted in many remarkable applications of great value. Many scientists think
that a basic understanding of science is necessary before an application is developed; therefore, applied science relies on
the results generated through basic science. Other scientists think that it is time to move on from basic science and instead
to find solutions to actual problems. Both approaches are valid. It is true that there are problems that demand immediate
attention; however, few solutions would be found without the help of the wide knowledge foundation generated through
basic science.
One example of how basic and applied science can work together to solve practical problems occurred after the discovery of
DNA structure led to an understanding of the molecular mechanisms governing DNA replication. Strands of DNA, unique
in every human, are found in our cells, where they provide the instructions necessary for life. During DNA replication,
DNA makes new copies of itself, shortly before a cell divides. Understanding the mechanisms of DNA replication enabled
scientists to develop laboratory techniques that are now used to identify genetic diseases. Without basic science, it is
unlikely that applied science would exist.
Another example of the link between basic and applied research is the Human Genome Project, a study in which each
human chromosome was analyzed and mapped to determine the precise sequence of DNA subunits and the exact location
of each gene. (The gene is the basic unit of heredity; an individual’s complete collection of genes is his or her genome.)
Other less complex organisms have also been studied as part of this project in order to gain a better understanding of human
chromosomes. The Human Genome Project (Figure 1.9) relied on basic research carried out with simple organisms and,
later, with the human genome. An important end goal eventually became using the data for applied research, seeking cures
and early diagnoses for genetically related diseases.

Figure 1.9 The Human Genome Project was a 13-year collaborative effort among researchers working in several
different fields of science. The project, which sequenced the entire human genome, was completed in 2003. (credit:
the U.S. Department of Energy Genome Programs (http://genomics.energy.gov))
While research efforts in both basic science and applied science are usually carefully planned, it is important to note that
some discoveries are made by serendipity, that is, by means of a fortunate accident or a lucky surprise. Penicillin was
discovered when biologist Alexander Fleming accidentally left a petri dish of Staphylococcus bacteria open. An unwanted
mold grew on the dish, killing the bacteria. The mold turned out to be Penicillium, and a new antibiotic was discovered.
Even in the highly organized world of science, luck—when combined with an observant, curious mind—can lead to
unexpected breakthroughs.
Reporting Scientific Work

Whether scientific research is basic science or applied science, scientists must share their findings in order for other
researchers to expand and build upon their discoveries. Collaboration with other scientists—when planning, conducting,
and analyzing results—are all important for scientific research. For this reason, important aspects of a scientist’s work are
communicating with peers and disseminating results to peers. Scientists can share results by presenting them at a scientific
meeting or conference, but this approach can reach only the select few who are present. Instead, most scientists present their
results in peer-reviewed manuscripts that are published in scientific journals. Peer-reviewed manuscripts are scientific
papers that are reviewed by a scientist’s colleagues, or peers. These colleagues are qualified individuals, often experts
in the same research area, who judge whether or not the scientist’s work is suitable for publication. The process of peer
review helps to ensure that the research described in a scientific paper or grant proposal is original, significant, logical, and
thorough. Grant proposals, which are requests for research funding, are also subject to peer review. Scientists publish their
work so other scientists can reproduce their experiments under similar or different conditions to expand on the findings. The
experimental results must be consistent with the findings of other scientists.
A scientific paper is very different from creative writing. Although creativity is required to design experiments, there are
fixed guidelines when it comes to presenting scientific results. First, scientific writing must be brief, concise, and accurate.
A scientific paper needs to be succinct but detailed enough to allow peers to reproduce the experiments.
The scientific paper consists of several specific sections—introduction, materials and methods, results, and discussion. This
structure is sometimes called the “IMRaD” format. There are usually acknowledgment and reference sections as well as an
abstract (a concise summary) at the beginning of the paper. There might be additional sections depending on the type of
paper and the journal where it will be published; for example, some review papers require an outline.
The introduction starts with brief, but broad, background information about what is known in the field. A good introduction
also gives the rationale of the work; it justifies the work carried out and also briefly mentions the end of the paper, where
the hypothesis or research question driving the research will be presented. The introduction refers to the published scientific
work of others and therefore requires citations following the style of the journal. Using the work or ideas of others without
proper citation is considered plagiarism.
The materials and methods section includes a complete and accurate description of the substances used, and the method
and techniques used by the researchers to gather data. The description should be thorough enough to allow another
researcher to repeat the experiment and obtain similar results, but it does not have to be verbose. This section will also
include information on how measurements were made and what types of calculations and statistical analyses were used to
examine raw data. Although the materials and methods section gives an accurate description of the experiments, it does not
discuss them.
Some journals require a results section followed by a discussion section, but it is more common to combine both. If the
journal does not allow the combination of both sections, the results section simply narrates the findings without any further
interpretation. The results are presented by means of tables or graphs, but no duplicate information should be presented.
In the discussion section, the researcher will interpret the results, describe how variables may be related, and attempt to
explain the observations. It is indispensable to conduct an extensive literature search to put the results in the context of
previously published scientific research. Therefore, proper citations are included in this section as well.
Finally, the conclusion section summarizes the importance of the experimental findings. While the scientific paper almost
certainly answered one or more scientific questions that were stated, any good research should lead to more questions.
Therefore, a well-done scientific paper leaves doors open for the researcher and others to continue and expand on the
findings.
Review articles do not follow the IMRAD format because they do not present original scientific findings, or primary
literature; instead, they summarize and comment on findings that were published as primary literature and typically include
extensive reference sections.
1.2 | Themes and Concepts of Biology

By the end of this section, you will be able to:
• Identify and describe the properties of life
• Describe the levels of organization among living things
• Recognize and interpret a phylogenetic tree
Connection for AP® Courses

The AP® Biology curriculum is organized around four major themes called the Big Ideas that apply to all levels of
biological organization—from molecules and cells to populations and ecosystems. Each Big Idea identifies key concepts
called Enduring Understandings, and Essential Knowledges, along with supporting examples. Simple descriptions define
the focus of each Big Idea: Big Idea 1, Evolution; Big Idea 2, Energy and Homeostasis; Big Idea 3, Information and
Communication; and Big Idea 4, Systems and Interactions. Evolution explains both the unity and diversity of life, Big Idea
1, and all organisms require energy and molecules to carry out life functions, such as growth and reproduction, Big Idea 2.
Living systems also store, transmit, and respond to information, from DNA sequences to nerve impulses and behaviors, Big
Idea 3. All biological systems interact, and these interactions result in emergent properties and characteristics unique to life,
Big Idea 4.
The redesigned AP® Biology course also emphasizes the investigative practices that students should master. Scientific
inquiry usually uses a series of steps to gain new knowledge. The scientific method begins with an observation and follows
with a hypothesis to explain the observation; then experiments are conducted to test the hypothesis, gather results, and draw
conclusions from data. The AP® program has identified seven major categories of Science Practices, which can be described
by short phrases: using representations and models to communicate information and solve problems; using mathematics
appropriately; engaging in questioning; planning and implementing data collection strategies; analyzing and evaluating
data; justifying scientific explanations; and connecting concepts. A Learning Objective merges content with one or more of
the seven Science Practices.
The information presented and the examples highlighted in this section support concepts and Learning Objectives outlined
in Big Idea 1 of the AP® Biology Curriculum. The Learning Objectives listed in the Curriculum Framework provide a
transparent foundation for the AP® Biology course, an inquiry-based laboratory experience, instructional activities, and
AP® Exam questions. A Learning Objective merges required content with one or more of the seven Science Practices.

Big Idea 1 The process of evolution drives the diversity and unity of life.
Enduring
Understanding Organisms are linked by lines of descent from common ancestry.
1.B
Essential 1.B.1 Organisms share many conserved core processes and features that evolved and are
Knowledge widely distributed among organisms today.
Science
3.1 The student can pose scientific questions.
Practice
Learning 1.14 The student is able to pose scientific questions that correctly identify essential
Objective properties of share, core life processes that provide insights into the history of life on Earth.
Essential 1.B.1 Organisms share many conserved core processes and features that evolved and are
Knowledge widely distributed among organisms today.
Science 5.3 The student can evaluate the evidence provided by data sets in relation to a particular
Practice scientific question.
Learning 1.18 The student is able to evaluate evidence provided by a data set in conjunction with a
Objective phylogenetic tree or simply cladogram to determine evolutionary history and speciation.
Biology is the science that studies life, but what exactly is life? This may sound like a silly question with an obvious
response, but it is not always easy to define life. For example, a branch of biology called virology studies viruses, which
exhibit some of the characteristics of living entities but lack others. It turns out that although viruses can attack living
organisms, cause diseases, and even reproduce, they do not meet the criteria that biologists use to define life. Consequently,
virologists are not biologists, strictly speaking. Similarly, some biologists study the early molecular evolution that gave rise
to life; since the events that preceded life are not biological events, these scientists are also excluded from biology in the
strict sense of the term.
From its earliest beginnings, biology has wrestled with three questions: What are the shared properties that make something
“alive”? And once we know something is alive, how do we find meaningful levels of organization in its structure? And,
finally, when faced with the remarkable diversity of life, how do we organize the different kinds of organisms so that we
can better understand them? As new organisms are discovered every day, biologists continue to seek answers to these and
other questions.
Properties of Life
All living organisms share several key characteristics or functions: order, sensitivity or response to the environment,
reproduction, adaptation, growth and development, regulation, homeostasis, energy processing, and evolution. When
viewed together, these nine characteristics serve to define life.
Order
Figure 1.10 A toad represents a highly organized structure consisting of cells, tissues, organs, and organ systems.
(credit: “Ivengo”/Wikimedia Commons)
Organisms are highly organized, coordinated structures that consist of one or more cells. Even very simple, single-celled
organisms are remarkably complex: inside each cell, atoms make up molecules; these in turn make up cell organelles and
other cellular inclusions. In multicellular organisms (Figure 1.10), similar cells form tissues. Tissues, in turn, collaborate to
create organs (body structures with a distinct function). Organs work together to form organ systems.
Sensitivity or Response to Stimuli
Figure 1.11 The leaves of this sensitive plant (Mimosa pudica) will instantly droop and fold when touched. After a few
minutes, the plant returns to normal. (credit: Alex Lomas)
Organisms respond to diverse stimuli. For example, plants can bend toward a source of light, climb on fences and walls, or
respond to touch (Figure 1.11). Even tiny bacteria can move toward or away from chemicals (a process called chemotaxis)
or light (phototaxis). Movement toward a stimulus is considered a positive response, while movement away from a stimulus
is considered a negative response.
Watch this video (http://openstaxcollege.org/l/movement_plants) to see how plants respond to a stimulus—from

opening to light, to wrapping a tendril around a branch, to capturing prey.
Which example most clearly shows a way that humans can respond directly to a change in the environment?
a. We shiver when we are cold and sweat when we are hot.
b. We walk by putting our front leg forward and pushing off with our back leg.
c. We are able to breath in and out unconsciously.
d. Our hair and fingernails grow at a constant rate over time.
Reproduction
Single-celled organisms reproduce by first duplicating their DNA, and then dividing it equally as the cell prepares to divide
to form two new cells. Multicellular organisms often produce specialized reproductive germline cells that will form new
individuals. When reproduction occurs, genes containing DNA are passed along to an organism’s offspring. These genes
ensure that the offspring will belong to the same species and will have similar characteristics, such as size and shape.
Growth and Development
Organisms grow and develop following specific instructions coded for by their genes. These genes provide instructions that

will direct cellular growth and development, ensuring that a species’ young (Figure 1.12) will grow up to exhibit many of
the same characteristics as its parents.
Figure 1.12 Although no two look alike, these kittens have inherited genes from both parents and share many of the
same characteristics. (credit: Rocky Mountain Feline Rescue)
Regulation
Even the smallest organisms are complex and require multiple regulatory mechanisms to coordinate internal functions,
respond to stimuli, and cope with environmental stresses. Two examples of internal functions regulated in an organism are
nutrient transport and blood flow. Organs (groups of tissues working together) perform specific functions, such as carrying
oxygen throughout the body, removing wastes, delivering nutrients to every cell, and cooling the body.
Homeostasis
Figure 1.13 Polar bears (Ursus maritimus) and other mammals living in ice-covered regions maintain their body
temperature by generating heat and reducing heat loss through thick fur and a dense layer of fat under their skin.
(credit: “longhorndave”/Flickr)
In order to function properly, cells need to have appropriate conditions such as proper temperature, pH, and appropriate
concentration of diverse chemicals. These conditions may, however, change from one moment to the next. Organisms
are able to maintain internal conditions within a narrow range almost constantly, despite environmental changes, through
homeostasis (literally, “steady state”). For example, an organism needs to regulate body temperature through a process
known as thermoregulation. Organisms that live in cold climates, such as the polar bear (Figure 1.13), have body structures
that help them withstand low temperatures and conserve body heat. Structures that aid in this type of insulation include fur,
feathers, blubber, and fat. In hot climates, organisms have methods (such as perspiration in humans or panting in dogs) that
help them to shed excess body heat.
Energy Processing
Figure 1.14 The California condor (Gymnogyps californianus) uses chemical energy derived from food to power flight.
California condors are an endangered species; this bird has a wing tag that helps biologists identify the individual.
(credit: Pacific Southwest Region U.S. Fish and Wildlife Service)
All organisms use a source of energy for their metabolic activities. Some organisms capture energy from the sun and convert
it into chemical energy in food; others use chemical energy in molecules they take in as food (Figure 1.14).
Activity
Select an ecosystem of your choice, such as a tropical rainforest, desert, or coral reef, and create a
representation to show how several organisms found in the ecosystem interact with each other and the
environment. Then, using similarities and differences among the organisms make a hypothesis about their
relatedness.
Consider the levels of organization of the biological world and create a diagram to place these items in order
from the smallest level of organization to the most encompassing: skin cell, planet Earth, elephant, tropical
rainforest, water molecule, liver, wolf pack, and oxygen atom. Justify the reason why you placed the items
in the hierarchy that you did.
Think About It
Homeostasis—the ability to “stay the same”—is a feature shared by all living organisms. You go for a long
walk on a hot day. Describe how homeostasis keeps your body healthy even though you are sweating
profusely. Then describe an example of an adaptation that evolved in a desert plant or animal that allows
them to survive in extreme temperatures.
Levels of Organization of Living Things

Living things are highly organized and structured, following a hierarchy that can be examined on a scale from small to
large. The atom is the smallest and most fundamental unit of matter. It consists of a nucleus surrounded by electrons. Atoms
form molecules. A molecule is a chemical structure consisting of at least two atoms held together by one or more chemical
bonds. Many molecules that are biologically important are macromolecules, large molecules that are typically formed by
polymerization (a polymer is a large molecule that is made by combining smaller units called monomers, which are simpler

than macromolecules). An example of a macromolecule is deoxyribonucleic acid (DNA) (Figure 1.15), which contains the
instructions for the structure and functioning of all living organisms.
Figure 1.15 All molecules, including this DNA molecule, are composed of atoms. (credit: “brian0918”/Wikimedia
Commons)
Watch this video (http://openstaxcollege.org/l/rotating_DNA) that animates the three-dimensional structure of the
DNA molecule shown in this figure.
The word helix means spiral. What does this tell you about the structure of DNA, which is a double helix
macromolecule?
a. The nucleotides of the two strands bond together with spiral bonds.
b. A double-stranded DNA molecule has two spiral stands bound together.
c. DNA is a double helix because it has two spiral strands held together like a spiral staircase.
d. Nucleotides are spiral-shaped molecules that bond together to form DNA.

Some cells contain aggregates of macromolecules surrounded by membranes; these are called organelles. Organelles are
small structures that exist within cells. Examples of organelles include mitochondria and chloroplasts, which carry out
indispensable functions: mitochondria produce energy to power the cell, while chloroplasts enable green plants to utilize
the energy in sunlight to make sugars. All living things are made of cells; the cell itself is the smallest fundamental unit of
structure and function in living organisms. (This requirement is why viruses are not considered living: they are not made of
cells. To make new viruses, they have to invade and hijack the reproductive mechanism of a living cell; only then can they
obtain the materials they need to reproduce.) Some organisms consist of a single cell and others are multicellular. Cells are
classified as prokaryotic or eukaryotic. Prokaryotes are single-celled or colonial organisms that do not have membrane-
bound nuclei; in contrast, the cells of eukaryotes do have membrane-bound organelles and a membrane-bound nucleus.
In larger organisms, cells combine to make tissues, which are groups of similar cells carrying out similar or related
functions. Organs are collections of tissues grouped together performing a common function. Organs are present not only
in animals but also in plants. An organ system is a higher level of organization that consists of functionally related organs.
Mammals have many organ systems. For instance, the circulatory system transports blood through the body and to and from
the lungs; it includes organs such as the heart and blood vessels. Organisms are individual living entities. For example,
each tree in a forest is an organism. Single-celled prokaryotes and single-celled eukaryotes are also considered organisms
and are typically referred to as microorganisms.
All the individuals of a species living within a specific area are collectively called a population. For example, a forest may
include many pine trees. All of these pine trees represent the population of pine trees in this forest. Different populations
may live in the same specific area. For example, the forest with the pine trees includes populations of flowering plants and
also insects and microbial populations. A community is the sum of populations inhabiting a particular area. For instance,
all of the trees, flowers, insects, and other populations in a forest form the forest’s community. The forest itself is an
ecosystem. An ecosystem consists of all the living things in a particular area together with the abiotic, non-living parts
of that environment such as nitrogen in the soil or rain water. At the highest level of organization (see this figure), the
biosphere is the collection of all ecosystems, and it represents the zones of life on earth. It includes land, water, and even
the atmosphere to a certain extent.

Figure 1.16 The biological levels of organization of living things are shown. From a single organelle to the entire
biosphere, living organisms are parts of a highly structured hierarchy. (credit “organelles”: modification of work by
Umberto Salvagnin; credit “cells”: modification of work by Bruce Wetzel, Harry Schaefer/ National Cancer Institute;
credit “tissues”: modification of work by Kilbad; Fama Clamosa; Mikael Häggström; credit “organs”: modification
of work by Mariana Ruiz Villareal; credit “organisms”: modification of work by "Crystal"/Flickr; credit “ecosystems”:
modification of work by US Fish and Wildlife Service Headquarters; credit “biosphere”: modification of work by
NASA)
Which of the following statements is false?

a. Tissues exist within organs which exist within organ systems.
b. Communities exist within populations which exist within ecosystems.
c. Organelles exist within cells which exist within tissues.
d. Communities exist within ecosystems which exist in the biosphere.
The Diversity of Life

The fact that biology, as a science, has such a broad scope has to do with the tremendous diversity of life on earth. The
source of this diversity is evolution, the process of gradual change during which new species arise from older species.
Evolutionary biologists study the evolution of living things in everything from the microscopic world to ecosystems.
The evolution of various life forms on Earth can be summarized in a phylogenetic tree (Figure 1.17). A phylogenetic tree is
a diagram showing the evolutionary relationships among biological species based on similarities and differences in genetic
or physical traits or both. A phylogenetic tree is composed of nodes and branches. The internal nodes represent ancestors
and are points in evolution when, based on scientific evidence, an ancestor is thought to have diverged to form two new
species. The length of each branch is proportional to the time elapsed since the split.
Figure 1.17 This phylogenetic tree was constructed by microbiologist Carl Woese using data obtained from
sequencing ribosomal RNA genes. The tree shows the separation of living organisms into three domains: Bacteria,
Archaea, and Eukarya. Bacteria and Archaea are prokaryotes, single-celled organisms lacking intracellular organelles.
(credit: Eric Gaba; NASA Astrobiology Institute)

Carl Woese and the Phylogenetic Tree

In the past, biologists grouped living organisms into five kingdoms: animals, plants, fungi, protists, and
bacteria. The organizational scheme was based mainly on physical features, as opposed to physiology,
biochemistry, or molecular biology, all of which are used by modern systematics. The pioneering work of
American microbiologist Carl Woese in the early 1970s has shown, however, that life on Earth has evolved
along three lineages, now called domains—Bacteria, Archaea, and Eukarya. The first two are prokaryotic
cells with microbes that lack membrane-enclosed nuclei and organelles. The third domain contains the
eukaryotes and includes unicellular microorganisms together with the four original kingdoms (excluding
bacteria). Woese defined Archaea as a new domain, and this resulted in a new taxonomic tree (see this
figure). Many organisms belonging to the Archaea domain live under extreme conditions and are called
extremophiles. To construct his tree, Woese used genetic relationships rather than similarities based on
morphology (shape).
Woese’s tree was constructed from comparative sequencing of the genes that are universally distributed,
present in every organism, and conserved (meaning that these genes have remained essentially unchanged
throughout evolution). Woese’s approach was revolutionary because comparisons of physical features are
insufficient to differentiate between the prokaryotes that appear fairly similar in spite of their tremendous
biochemical diversity and genetic variability (Figure 1.18). The comparison of homologous DNA and RNA
sequences provided Woese with a sensitive device that revealed the extensive variability of prokaryotes,
and which justified the separation of the prokaryotes into two domains: bacteria and archaea.
Figure 1.18 These images represent different domains. The (a) bacteria in this micrograph belong to Domain
Bacteria, while the (b) extremophiles (not visible) living in this hot vent belong to Domain Archaea. Both the
(c) sunflower and (d) lion are part of Domain Eukarya. (credit a: modification of work by Drew March; credit b:
modification of work by Steve Jurvetson; credit c: modification of work by Michael Arrighi; credit d: modification of
work by Leszek Leszcynski)
In which domain would a fish be classified? Why?

a. Archaea, because fish are multicellular.
b. Eukarya, because fish are multicellular.
c. Archaea, because fish are single-celled.
d. Eukarya because fish are single-celled.

Phylogenetic trees can represent traits that are derived or lost due to evolution. One example is the
absence of legs in some sea mammals. For example, Cetaceans are marine mammals that include toothed
whales, such as dolphins and killer whales, and baleen whales, such as humpback whales. Cetaceans are
descended from even-toed ungulates and share a common ancestry with the hippopotamus, cow, sheep,
camel, and pig.
Figure 1.19
Phylogenetic trees can represent traits that are derived or lost due to evolution. One example is the absence of legs in
some marine mammals. One such group is the Cetaceans, which includes toothed whales, such as dolphins and killer
whales, and baleen whales, such as humpback whales. Cetaceans are descended from even-toed ungulates and share a
common ancestry with the hippopotamus, cows, sheep, camel, and pig. Based on this phylogenetic tree, which of the
following animal is the most closely related to a horse?
a. an armadillo
b. a camel
c. a bat
d. a cat
Branches of Biological Study

The scope of biology is broad and therefore contains many branches and subdisciplines. Biologists may pursue one of
those subdisciplines and work in a more focused field. For instance, molecular biology and biochemistry study biological
processes at the molecular and chemical level, including interactions among molecules such as DNA, RNA, and proteins,
as well as the way they are regulated. Microbiology, the study of microorganisms, is the study of the structure and function
of organisms that cannot be seen with the naked eye. It is quite a broad branch itself, and depending on the subject of study,
there are also microbial physiologists, ecologists, and geneticists, among others.

Forensic science is the application of science to answer questions related to the law. Biologists as well
as chemists and biochemists can be forensic scientists. Forensic scientists provide scientific evidence for
use in courts, and their job involves examining trace materials associated with crimes. Interest in forensic
science has increased in the last few years, possibly because of popular television shows that feature
forensic scientists on the job. Also, the development of molecular techniques and the establishment of DNA
databases have expanded the types of work that forensic scientists can do. Their work involves analyzing
samples such as hair, blood, and other body fluids and also processing DNA (Figure 1.20) found in many
different environments and materials. Forensic scientists also analyze other biological evidence left at crime
scenes, such as insect larvae or pollen grains. Students who want to pursue careers in forensic science will
most likely be required to take chemistry and biology courses as well as some intensive math courses.
Figure 1.20 This forensic scientist works in a DNA extraction room at the U.S. Army Criminal Investigation
Laboratory at Fort Gillem, GA. (credit: United States Army CID Command Public Affairs)
Another field of biological study, neurobiology, studies the biology of the nervous system, and although it is considered
a branch of biology, it is also recognized as an interdisciplinary field of study known as neuroscience. Because of its
interdisciplinary nature, this subdiscipline studies different functions of the nervous system using molecular, cellular,
developmental, medical, and computational approaches.
Figure 1.21 Researchers work on excavating dinosaur fossils at a site in Castellón, Spain. (credit: Mario Modesto)
Paleontology, another branch of biology, uses fossils to study life’s history (Figure 1.21). Zoology and botany are the
study of animals and plants, respectively. Biologists can also specialize as biotechnologists, ecologists, or physiologists, to
name just a few areas. This is just a small sample of the many fields that biologists can pursue.
Biology is the culmination of the achievements of the natural sciences from their inception to today. Excitingly, it is the
cradle of emerging sciences, such as the biology of brain activity, genetic engineering of custom organisms, and the biology
of evolution that uses the laboratory tools of molecular biology to retrace the earliest stages of life on earth. A scan of
news headlines—whether reporting on immunizations, a newly discovered species, sports doping, or a genetically-modified
food—demonstrates the way biology is active in and important to our everyday world.

KEY TERMS
abstract opening section of a scientific paper that summarizes the research and conclusions
applied science form of science that aims to solve real-world problems
atom smallest and most fundamental unit of matter
basic science science that seeks to expand knowledge and understanding regardless of the short-term application of that
knowledge
biochemistry study of the chemistry of biological organisms
biology the study of living organisms and their interactions with one another and their environments
biosphere collection of all the ecosystems on Earth
botany study of plants
cell smallest fundamental unit of structure and function in living things
community set of populations inhabiting a particular area
conclusion section of a scientific paper that summarizes the importance of the experimental findings
control part of an experiment that does not change during the experiment
deductive reasoning form of logical thinking that uses a general inclusive statement to forecast specific results
descriptive science (also, discovery science) form of science that aims to observe, explore, and investigate
discussion section of a scientific paper in which the author interprets experimental results, describes how variables may
be related, and attempts to explain the phenomenon in question
ecosystem all the living things in a particular area together with the abiotic, nonliving parts of that environment
eukaryote organism with cells that have nuclei and membrane-bound organelles
evolution process of gradual change during which new species arise from older species and some species become extinct
falsifiable able to be disproven by experimental results
homeostasis ability of an organism to maintain constant internal conditions
hypothesis suggested explanation for an observation, which can be tested
hypothesis-based science form of science that begins with a specific question and potential testable answers
inductive reasoning form of logical thinking that uses related observations to arrive at a general conclusion
introduction opening section of a scientific paper, which provides background information about what was known in the
field prior to the research reported in the paper
life science field of science, such as biology, that studies living things
macromolecule large molecule, typically formed by the joining of smaller molecules
materials and methods section of a scientific paper that includes a complete description of the substances, methods,
and techniques used by the researchers to gather data
microbiology study of the structure and function of microorganisms
molecular biology study of biological processes and their regulation at the molecular level, including interactions among
molecules such as DNA, RNA, and proteins
molecule chemical structure consisting of at least two atoms held together by one or more chemical bonds
natural science field of science that is related to the physical world and its phenomena and processes
neurobiology study of the biology of the nervous system
organ collection of related tissues grouped together performing a common function
organ system level of organization that consists of functionally related interacting organs
organelle small structures that exist within cells and carry out cellular functions
organism individual living entity
paleontology study of life’s history by means of fossils
peer-reviewed manuscript scientific paper that is reviewed by a scientist’s colleagues who are experts in the field of
study
phylogenetic tree diagram showing the evolutionary relationships among various biological species based on
similarities and differences in genetic or physical traits or both; in essence, a hypothesis concerning evolutionary
connections
physical science field of science, such as geology, astronomy, physics, and chemistry, that studies nonliving matter
plagiarism using other people’s work or ideas without proper citation, creating the false impression that those are the
author’s original ideas
population all of the individuals of a species living within a specific area
prokaryote single-celled organism that lacks organelles and does not have nuclei surrounded by a nuclear membrane
results section of a scientific paper in which the author narrates the experimental findings and presents relevant figures,
pictures, diagrams, graphs, and tables, without any further interpretation
review article paper that summarizes and comments on findings that were published as primary literature
science knowledge that covers general truths or the operation of general laws, especially when acquired and tested by the
scientific method
scientific method method of research with defined steps that include observation, formulation of a hypothesis, testing,
and confirming or falsifying the hypothesis
serendipity fortunate accident or a lucky surprise
theory tested and confirmed explanation for observations or phenomena
tissue group of similar cells carrying out related functions
variable part of an experiment that the experimenter can vary or change
zoology study of animals
CHAPTER SUMMARY
1.1 The Science of Biology
Biology is the science that studies living organisms and their interactions with one another and their environments. Science
attempts to describe and understand the nature of the universe in whole or in part by rational means. Science has many
fields; those fields related to the physical world and its phenomena are considered natural sciences.

Science can be basic or applied. The main goal of basic science is to expand knowledge without any expectation of short-
term practical application of that knowledge. The primary goal of applied research, however, is to solve practical
problems.
Two types of logical reasoning are used in science. Inductive reasoning uses particular results to produce general scientific
principles. Deductive reasoning is a form of logical thinking that predicts results by applying general principles. The
common thread throughout scientific research is the use of the scientific method, a step-based process that consists of
making observations, defining a problem, posing hypotheses, testing these hypotheses, and drawing one or more
conclusions. The testing uses proper controls. Scientists present their results in peer-reviewed scientific papers published
in scientific journals. A scientific research paper consists of several well-defined sections: introduction, materials and
methods, results, and, finally, a concluding discussion. Review papers summarize the research done in a particular field
over a period of time.
1.2 Themes and Concepts of Biology
Biology is the science of life. All living organisms share several key properties such as order, sensitivity or response to
stimuli, reproduction, growth and development, regulation, homeostasis, and energy processing. Living things are highly
organized parts of a hierarchy that includes atoms, molecules, organelles, cells, tissues, organs, and organ systems.
Organisms, in turn, are grouped as populations, communities, ecosystems, and the biosphere. The great diversity of life
today evolved from less-diverse ancestral organisms over billions of years. A diagram called a phylogenetic tree can be
used to show evolutionary relationships among organisms.
Biology is very broad and includes many branches and subdisciplines. Examples include molecular biology, microbiology,
neurobiology, zoology, and botany, among others.
REVIEW QUESTIONS
1. What is a suggested and testable explanation for an a. diseases affecting humans, pollution affecting
event called? species habitat,
calculating surface area of rectangular ground,
a. discovery
functioning of planetary orbitals
b. hypothesis
b. calculating surface area of rectangular ground,
c. scientific method functioning of planetary orbitals,
d. theory formation of metamorphic rocks, galaxy
formation and evolution
2. Which of the following sciences is not considered a
natural science? c. plant responses to external stimuli, functioning
of planetary orbitals,
a. Astronomy formation of metamorphic rocks, galaxy
b. Biology formation and evolution
c. Computer science d. plant responses to external stimuli, study of the
shape and motion of physical objects,
d. Physics
formation of metamorphic rocks, galaxy
3. What is the name for the formal process through which formation and evolution
scientific research is checked for originality, significance,
5. Which of the following is an example of deductive
and quality before being accepted into scientific literature?
reasoning?
a. Most swimming animals use fins; therefore, fins
a. publication
are an adaptation to swimming.
b. public speaking
b. Mitochondria are inherited from the mother;
c. peer review therefore, maternally inherited traits are encoded
d. the scientific method by mitochondrial DNA
4. What are two topics that are likely to be studied by c. Small animals lose more heat than larger
biologists and two areas of scientific study that would fall animals. One would not expect to find wild mice
outside the realm of biology? in the poles.
d. Water conservation is a major requirement to
survive in the desert. Long leaves increase loss
of water by evaporation. Therefore, desert plants
should have smaller leaves.
6. Why are viruses not considered living? a. biosphere, ecosystem, community, population,
organism
a. They are not made of cells.
b. organelle, tissue, biosphere, ecosystem,
b. Viruses do not have genetic material.
population
c. Viruses have DNA and RNA.
c. organism, organ, tissue, organelle, molecule
d. Viruses are obligate parasites and require a host.
d. organism, community, biosphere, molecule,
7. The presence of a membrane-enclosed nucleus is a tissue, organ
characteristic of what?
10. Where in a phylogenetic tree would you expect to find
a. bacteria the organism that had evolved most recently?
b. eukaryotic cells a. at the base
c. all living organisms b. at the nodes
d. prokaryotic cells c. at the branch tips
8. What is a group of individuals of the same species d. within the branches
living in the same area called?
11. What is a characteristic that is not present in all living
a. a community things?
b. an ecosystem a. homeostasis and regulation
c. a family b. metabolism
d. a population c. nucleus containing DNA
9. Which of the following sequences represents the d. reproduction
hierarchy of biological organization from the most
inclusive to the least complex level?
CRITICAL THINKING QUESTIONS

12. Is mathematics a natural science? Explain your a. 1, 2, 3, 4, 5, 6
reasoning. b. 2, 1, 3, 4, 5, 6
a. No, it is not a natural science because it is not c. 2, 1, 5, 4, 6, 3
used in the study of the natural world.
d. 2, 1, 5, 6, 3, 4
b. No, it is not a natural science. Mathematics
focuses on understanding mathematical relations 14. Read the following questions. Does the statement lend
and calculations, which is useful in natural itself to investigation using the scientific method? In other
sciences but which is distinct. words, is the hypothesis falsifiable (can be proven false)?
1. Is macaroni and cheese tastier than broccoli soup?
c. Yes, it is a natural science. Mathematics deals
with verifying the experimental data. 2. Are hummingbirds attracted to the color red?
d. Yes, it is a natural science. It uses chemical and 3. Is the moon made out of green cheese?
physical measurements. 4. Is plagiarism dishonest?
13. Although the scientific method is used by most of the a. Questions 1 and 2 are subjective and cannot be
sciences, it can also be applied to everyday situations. A disproven using scientific method. Questions 3
situation is given below. Using the scientific method try to and 4 can be tested using scientific method.
arrange the given steps in the correct order. Situation:
1. If the car doesn’t start the problem might be in the b. Questions 3 and 4 are subjective and cannot be
battery. disproven using scientific method. Questions 1
and 2 can be tested using scientific method.
2. Car doesn’t start.
c. Questions 1 and 3 are subjective and cannot be
3. After changing the battery. Car starts working. disproven using scientific method. Questions 2
4. The car should start after charging the battery or and 4 can be tested using scientific method.
changing the battery. d. Questions 1 and 4 are subjective and cannot be
5. The car doesn’t start because the battery is dead. disproven using scientific method. Questions 2
and 3 can be tested using scientific method.
6. The car doesn’t start even after charging the battery,
the battery must have stopped working. 15. Consider the levels of organization of the biological

world and place each of these items in order from smallest a. a sequence of DNA
level of organization to most encompassing: skin cell, b. a sequence of rRNA
elephant, water molecule, planet Earth, tropical rainforest,
hydrogen atom, wolf pack, liver. c. a sequence of mRNA.
a. hydrogen atom, water molecule, skin cell, liver, d. a sequence of tRNA.
elephant, wolf pack, tropical rainforest, planet 17. Both astronomy and astrology study the stars. Which
Earth one is considered a natural science? Explain your
b. hydrogen atom, skin cell, water molecule, liver, reasoning.
elephant, wolf pack, tropical rainforest, planet a. Astrology is a natural science as it indirectly
Earth influences human affairs and the natural world.
c. hydrogen atom, skin cell, water molecule, liver, b. Astronomy is a natural science as it deals with
wolf pack, elephant, tropical rainforest, planet observations and prediction of events in the sky,
Earth which is based on the laws of physics.
d. water molecule, hydrogen atom, skin cell, liver, c. Astrology is a natural science as it deals with
elephant, wolf pack, tropical rainforest, planet observations and prediction of events in the sky,
Earth influences human affairs and the natural world.
16. What scientific evidence was used by Carl Woese to d. Astrology is a natural science as it deals with the
determine there should be a separate domain for Archaea? study of asteroids and comets, which is based on
the laws of natural sciences.
TEST PREP FOR AP® COURSES

18. Which of the following structures is conserved in all distantly related to 2?
living organisms and points to a common origin?
a. 1
a. All living organisms have mitochondria that
b. 3
produce energy.
c. 4
b. All living organisms store genetic material in
DNA/RNA. d. 5
c. All living organisms use the energy from 21.
sunlight
d. All living organisms have a nucleus.
19. Which of the following statements is the strongest
argument in favor of two organisms, A and B, being
closely related evolutionarily?
a. A and B look alike.
b. A and B live in the same ecosystem.
c. A and B use the same metabolic pathways.
d. The DNA sequences of A and B are highly In the diagram shown which is the most recent common
homologous. ancestor of 1 and 3?
20. a. A
b. B
c. C
d. D
22. The French scientist Jacques Monod famously said,
“Anything found to be true of E. coli must also be true of
elephants.” How is this statement based on the notion that
living organisms share a common ancestor?
In the phylogenetic tree shown, which organism is most

a. E. coli is a eukaryote and share similarities with line of evidence that has led to this reclassification?
most of the living organisms. a. Archeopteryx is the connecting link between
b. E. coli is a prokaryote. The various metabolic birds and reptiles which shows that birds and
processes and core functions in E. coli share reptiles are related.
homology with higher organisms. b. Birds have scales, having the same origin as that
c. E. coli contains a nucleus and membrane bound of reptiles.
cell organelles that are shared by all the living c. Birds and reptiles have the same circulatory and
organisms. excretory systems and both are egg laying
d. E. coli is a prokaryote and reproduces through animals.
binary fission which is common to most of the d. Birds and reptiles have similar anatomical and
living organisms. morphological features.
23. Birds have been reclassified as reptiles. What is one

Chapter 1. The Study of Life

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Biology for AP® Courses

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1 | THE STUDY OF LIFE

1.1 | The Science of Biology

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The Process of Science

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Two Types of Science: Basic Science and Applied Science

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Reporting Scientific Work

1.2 | Themes and Concepts of Biology

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Levels of Organization of Living Things

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Which of the following statements is false?

The Diversity of Life

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Carl Woese and the Phylogenetic Tree

In which domain would a fish be classified? Why?

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Branches of Biological Study

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applied science form of science that aims to solve real-world problems

atom smallest and most fundamental unit of matter

biochemistry study of the chemistry of biological organisms

biosphere collection of all the ecosystems on Earth

botany study of plants

cell smallest fundamental unit of structure and function in living things

community set of populations inhabiting a particular area

falsifiable able to be disproven by experimental results