I would like to express my special thanks of

gratitude to my Chemistry teacher Ms. Shilpa

Bhatia for her able guidance and support in
completing my project.
I would also like to extend my gratitudeto our
respected Principal Sir Mr. Jagdish Sharma
and Vice-Principal Ma’am Ms. Rachna Arora
for providing me with all the facilities that
were required to complete this Chemistry
project.

Name: Ritika
Class: XII
Sec: D
This is to certify that the content of the
Project is the bonafide work of Ritika of
class XII and is submitted to me for partial
fulfilment of requirement of CBSE.
The original research work was carried out
by her under my supervision in the academic
year 2022-23. Based on the declaration made
by her, I recommend this project for
evaluation.

Shilpa Bhatia
PGT chemistry
Sr. No Content
1. Aim
2. Introduction
3 Requirement
4. General Procedure
5. Experiment
(i) Aim
(ii) Material Required
(iii) Procedure
(iv) Observations
(v) Result
6. Conclusion
7. Bibliography
8. Science Quotation
To determine the quantity of casein protein
present in milk.
Milk is a complete diet as it contains proteins,
carbohydrates, fats and minerals.

As casein it exists in the milk, it is a salt of

calcium.
It is relatively hydrophobic, making it poorly
soluble in water. While it is also insoluble in
neutral salt solutions, it is readily dispersible in
dilute alkali and in salt solutions such as sodium
and sodium acetate. It is found in milk as a
suspension of particles called casein micelles.
➢ Laptop
➢ Wi-Fi Connection
➢ Stationery Material
➢ Paper, pen, stick file
➢I undertook the extensive research work to
determine the quantity of casein in
different samples of milk thoroughly.

➢ During this process, I have examined

different research papers published online
as well as in different books.

➢ The photographs of the experiment

conducted in chemistry lab are attached
herewith for reference.

➢I shared my knowledge and experience

after conducting experiment related to my
investigatory project.
 AIM:
To determine the quantity of casein in
different samples of milk.
➢ Funnel
➢ Funnel stand
➢ Glass rod
➢ Filter paper
➢ Weight box
➢ Test tubes
➢ Mortar and pestle
➢ Different samples of milk
➢ Saturated ammonium sulphate solution
➢ 10% acetic acid solution
➢ Wash the beaker (250 mL) with the
distilled water and dry it.
➢ Take 20 mL of buffalo’s milk in 250
mL beaker and find it’s weight.
➢ Warm the contents of the beaker to
40-45 degree celsius on a low flame.
➢ Now add 10% acetic acid solution
drop wise with stirring.
➢ Leave the beaker undisturbed for 15
minutes so that casein will get
precipitated easily.
➢ Filter the above solution and transfer
the precipitate in another beaker.
➢ Find the weight of dry precipitate.
➢ Repeat the whole experiment with
cow’s milk, buffalo’s milk and amul
milk.
Volume of milk taken in each case = 20 mL
Weight of milk taken = W1 g
Weight of casein isolated = W2 g

Percentage of casein = W2 ×100

W1

S.No Type Volume Weight % of

of Milk of Milk of Casein
Casein
1. Buffalo 20 mL 2.73 g 4.2%
2. Cow 20 mL 1.64 g 3%
3. Amul 20 mL 2.88 g 3.18%
Warming the contents of
beaker

Stirring the contents with

glass rod
Filtering the precipitate

Repeating the experiments

with other samples of milk
Different samples of milk contain different
percentage of casein. Highest percentage of
casein is present in Buffalo’s milk.
According to above brief analysis of casein in
milk, we are able to conclude that:
➢ Amount of casein in buffalo’s milk is 4.2 %
➢ Amount of casein in cows milk is 3%
➢ Amount of casein milk in amul milk is
3.18%

So according to our analysis we can finally

conclude that buffalo’s milk is the best milk or
the beneficial milk for the human beings.
➢ https://www.icbse.com/projects/chemistry-project-to-
study-the-quantity-of-caesin-in-milk-jmn

➢ https://www.scribd.com/document/399241943/Study-
of-Quantity-of-Casein-Present-in-Different-Samples-of-
Milk-Chemistry-Investigatory-Project-Class-XII-CBSE-
SANJIB

➢ https://www.biosciencenotes.com/casein/

➢ https://www.seminarsonly.com/Engineering-
Projects/Chemistry/Determine-the-Quantity-of-Casein-
in-Milk.php

➢ https://thedairydish.com/cottage-cheese-casein-
benefits/
Chemistry is the
study of matter ,
But I prefer to
see it as the
study of change.
-Walter White